尖孢镰刀菌PO-Yi对蔬菜土壤中邻苯二甲酸酯的降解作用（英文）
|
摘要
试验模拟低中高3个污染水平、6种PAEs(DMP、DEP、DIBP、DBP、BBP和DEHP)的污染,利用课题组前期筛选出的高效降解真菌,尖孢镰刀菌PO-Yi (Fusarium oxysporum),通过盆栽试验进一步研究其对蔬菜(辣椒、茄子)土壤中PAEs的降解作用。结果表明, PO-Yi可以不同程度地促进蔬菜(辣椒和茄子)土壤中PAEs的降解,其中绝对降解率最高的为DEP,在20 mg/kg的PAEs污染水平时,达39.5%,比10 mg/kg的PAEs提高了14.2%,对辣椒土壤中的降解效果要比茄子土壤中的降解效果好;尖孢镰刀菌PO-Yi菌对3种污染程度不同的辣椒土壤和茄子土壤都有良好的生物修复表现,30d内能将总量60 mg/kg PAEs降解76.8%和63.1%;菌株PO-Yi、土著微生物和不同蔬菜作物(辣椒与茄子)在PAEs复合污染的盆栽土壤中表现出对土壤PAEs降解的协同效应。
In this research, soil samples were first polluted with 6 PAEs(i.e. DMP,DEP, DIBP, DBP, DEHP, and BBP) at 3 different concentration gradients(0, 10, and20 mg/kg) and then treated with a highly-efficient degradation fungus, Fusarium oxysporum(PO-Yi), to investigate the biodegradation of PAEs in pepper and eggplant soil. The findings revealed that PO-Yi can accelerate the degradation of PAEs in vegetable soils including pepper and eggplant soil to varying degrees. The highest absolute degradation rate(up to 39.5%) was observed in DEP-treated soils at the high pollution level(20 mg/kg), which was 14.2% higher than that at the middle pollution level(10 mg/kg). The degradation effect in the pepper soil was superior to that in the eggplant soil. Vegetable soil, either pepper soil or eggplant soil, which was polluted by various PAEs at different pollution gradients and then treated with PO-Yi fungus presented good bioremediation results. In the pepper and eggplant soil, 76.8%and 63.1% of the PAEs with a total volume of 60 mg/kg were degraded within 30 d respectively. PO-Yi, indigenous microorganisms, and the vegetables, i.e. pepper and eggplant had good synergistic effects on the degradation of compound PAEs in PAEpolluted pot soil.
In this research, soil samples were first polluted with 6 PAEs(i.e. DMP,DEP, DIBP, DBP, DEHP, and BBP) at 3 different concentration gradients(0, 10, and20 mg/kg) and then treated with a highly-efficient degradation fungus, Fusarium oxysporum(PO-Yi), to investigate the biodegradation of PAEs in pepper and eggplant soil. The findings revealed that PO-Yi can accelerate the degradation of PAEs in vegetable soils including pepper and eggplant soil to varying degrees. The highest absolute degradation rate(up to 39.5%) was observed in DEP-treated soils at the high pollution level(20 mg/kg), which was 14.2% higher than that at the middle pollution level(10 mg/kg). The degradation effect in the pepper soil was superior to that in the eggplant soil. Vegetable soil, either pepper soil or eggplant soil, which was polluted by various PAEs at different pollution gradients and then treated with PO-Yi fungus presented good bioremediation results. In the pepper and eggplant soil, 76.8%and 63.1% of the PAEs with a total volume of 60 mg/kg were degraded within 30 d respectively. PO-Yi, indigenous microorganisms, and the vegetables, i.e. pepper and eggplant had good synergistic effects on the degradation of compound PAEs in PAEpolluted pot soil.
引文
[1]LING B(凌波).Effects of environmental endocrine disruptors on human health(环境内分泌干扰物的健康影响).Chinese Journal of Public Health(中国公共卫生),2002,18(2):37-239.
[2]PROK?PKOVáG,HOLADOVáK,POUSTKA J,et al.Development of a solid-phase microextraction method for the determination of phthalic acid esters in water.Analytica Chimica Acta,2002,457(2):211-223.
[3]BLOUNT B C,MILGRAM K E,SILVA M J,et al.Quantitative detection of eight phthalate metabolites in human urine using HPLC-APCI-MS/MS.Analytical Chemistry,2000,72(17):4127-4134.
[4]ZHANG J(张建),SHI Y J(石义静),CUI Y(崔寅),et al.Phthalate esters degradation and effects on enzyme activity in soil(土壤中邻苯二甲酸酯类物质的降解及其对土壤酶活性的影响).Environmental science(环境科学),2010,31(12):3056-3061.
[5]HUANG C,ZHU J,CHEN L,et al.Structural changes and plasticizer migration of starch-based food packaging material contacting with milk during microwave heating.Food Control,2014,36(1):55-62.
[6]BOMFIM M V J,ZAMITH H P S,ABRANTES S M P.Migration ofε-caprolactam residues in packaging intended for contact with fatty foods.Food Control,2011,22(5):681-684.
[7]FASANO E,BONO-BLAY F,CIRILLO T,et al.Migration of phthalates,alkylphenols,bisphenol A and di(2-ethylhexyl)adipate from food packaging.Food Control,2012,27(1):132-138.
[8]AFSHARI A,GUNNARSEN L,CLAUSEN P A,et al.Emission of phthalates from PVC and other materials.Indoor Air,2004,14(2):120-128.
[9]FROMME H,LAHRZ T,PILOTY M,et al.Occurrence of phthalates and musk fragrances in indoor air and dust from apartments and kindergartens in Berlin(Germany).Indoor Air,2004,14(3):188-195.
[10]FUJII M,SHINOHARA N,LIM A,et al.A study on emission of phthalate esters from plastic materials using a passive flux sampler.Atmospheric Environment,2003,37(39-40):5495-5504.
[11]HIGUCHI T T,PALMER J S,GRAY L E Jr,et al.Effects of dibutyl phthalate in male rabbits following in utero,adolescent,or postpubertal exposure.Toxicological Sciences,2003,72(2):301-313.
[12]HERREROó,PLANELLóR,MORCILLO G.The plasticizer benzyl butyl phthalate(BBP)alters the ecdysone hormone pathway,the cellular response to stress,the energy metabolism,and several detoxication mechanisms in Chironomus riparius larvae.Chemosphere,2015,128:266-277.
[13]WANG J L,ZHAO X,WU W Z.Biodegradation of phthalic acid esters(PAEs)in soil bioaugmented with acclimated activated sludge.Process Biochemistry,2004,39(12):1837-1841.
[14]MACKINTOSH C E,MALDONADO J A,IKONOMOU M G,et al.Sorption of phthalate esters and PCBs in a marine ecosystem.Environmental Science&Technology,2006,40(11):3481-3488.
[15]WANG J L,YE YC,WU W Z.Comparison of Di-n-methyl Phthalate Biodegradation by Free and Immobilized Microbial Cells.Biomedical and Environmental Sciences,2003,16(2):126-132.
[16]QIN H(秦华),LIN X G(林先贵),YIN R(尹睿),et al.Effects of Arbuscular mycorrhizal fungi and two-strain bacteria on mung bean growth and DEHP degradation in soil(丛枝菌根真菌和两株细菌对土壤中DEHP降解及绿豆生长的影响).Environmental science(环境科学学报),2006,26(10):1651-1657.
[17]GUO Y(郭杨),HAN R(韩蕊),CAI X D(蔡信德),et al.Effects of phthalate esters compound pollution on soil microbial ecology(邻苯二甲酸酯复合污染对土壤微生态的影响).Research of environmental science(环境科学研究),2010,23(11):1410-1414.
[18]QIN H(秦华),LIN X G(林先贵),CHEN R R(陈瑞蕊),et al.Effects of DEHP on dehydrogenase activity and microbial functional diversity in soil(DEHP对土壤脱氢酶活性及微生物功能多样性的影响).Acta pedologica sinica(土壤学报),2005,42(5):829-834.
[19]LIANG W,DENG J Q,ZHAN F C,et al.Effects of constructed wetland system on the removal of dibutyl phthalate(DBP).Microbiological Research,2009,164(2):206-211.
[20]XU X R,LI H B,GU J D.Biodegradation of an endocrinedisrupting chemical di-n-butyl phthalate ester by Pseudomonas fluorescens B-1.International Biodeterioration&Biodegradation,2005,55(1):9-15.
[21]HAN R(韩蕊),WANG D Y(王冬莹),RUI Y(芮洋),et al.Screen and property analysis of a fungus capable of degrading phthalate esters(一株降解邻苯二甲酸酯真菌的筛选及其降解特性研究).Environmental science(环境科学学报),2013,33(11):2941-2946.
[2]PROK?PKOVáG,HOLADOVáK,POUSTKA J,et al.Development of a solid-phase microextraction method for the determination of phthalic acid esters in water.Analytica Chimica Acta,2002,457(2):211-223.
[3]BLOUNT B C,MILGRAM K E,SILVA M J,et al.Quantitative detection of eight phthalate metabolites in human urine using HPLC-APCI-MS/MS.Analytical Chemistry,2000,72(17):4127-4134.
[4]ZHANG J(张建),SHI Y J(石义静),CUI Y(崔寅),et al.Phthalate esters degradation and effects on enzyme activity in soil(土壤中邻苯二甲酸酯类物质的降解及其对土壤酶活性的影响).Environmental science(环境科学),2010,31(12):3056-3061.
[5]HUANG C,ZHU J,CHEN L,et al.Structural changes and plasticizer migration of starch-based food packaging material contacting with milk during microwave heating.Food Control,2014,36(1):55-62.
[6]BOMFIM M V J,ZAMITH H P S,ABRANTES S M P.Migration ofε-caprolactam residues in packaging intended for contact with fatty foods.Food Control,2011,22(5):681-684.
[7]FASANO E,BONO-BLAY F,CIRILLO T,et al.Migration of phthalates,alkylphenols,bisphenol A and di(2-ethylhexyl)adipate from food packaging.Food Control,2012,27(1):132-138.
[8]AFSHARI A,GUNNARSEN L,CLAUSEN P A,et al.Emission of phthalates from PVC and other materials.Indoor Air,2004,14(2):120-128.
[9]FROMME H,LAHRZ T,PILOTY M,et al.Occurrence of phthalates and musk fragrances in indoor air and dust from apartments and kindergartens in Berlin(Germany).Indoor Air,2004,14(3):188-195.
[10]FUJII M,SHINOHARA N,LIM A,et al.A study on emission of phthalate esters from plastic materials using a passive flux sampler.Atmospheric Environment,2003,37(39-40):5495-5504.
[11]HIGUCHI T T,PALMER J S,GRAY L E Jr,et al.Effects of dibutyl phthalate in male rabbits following in utero,adolescent,or postpubertal exposure.Toxicological Sciences,2003,72(2):301-313.
[12]HERREROó,PLANELLóR,MORCILLO G.The plasticizer benzyl butyl phthalate(BBP)alters the ecdysone hormone pathway,the cellular response to stress,the energy metabolism,and several detoxication mechanisms in Chironomus riparius larvae.Chemosphere,2015,128:266-277.
[13]WANG J L,ZHAO X,WU W Z.Biodegradation of phthalic acid esters(PAEs)in soil bioaugmented with acclimated activated sludge.Process Biochemistry,2004,39(12):1837-1841.
[14]MACKINTOSH C E,MALDONADO J A,IKONOMOU M G,et al.Sorption of phthalate esters and PCBs in a marine ecosystem.Environmental Science&Technology,2006,40(11):3481-3488.
[15]WANG J L,YE YC,WU W Z.Comparison of Di-n-methyl Phthalate Biodegradation by Free and Immobilized Microbial Cells.Biomedical and Environmental Sciences,2003,16(2):126-132.
[16]QIN H(秦华),LIN X G(林先贵),YIN R(尹睿),et al.Effects of Arbuscular mycorrhizal fungi and two-strain bacteria on mung bean growth and DEHP degradation in soil(丛枝菌根真菌和两株细菌对土壤中DEHP降解及绿豆生长的影响).Environmental science(环境科学学报),2006,26(10):1651-1657.
[17]GUO Y(郭杨),HAN R(韩蕊),CAI X D(蔡信德),et al.Effects of phthalate esters compound pollution on soil microbial ecology(邻苯二甲酸酯复合污染对土壤微生态的影响).Research of environmental science(环境科学研究),2010,23(11):1410-1414.
[18]QIN H(秦华),LIN X G(林先贵),CHEN R R(陈瑞蕊),et al.Effects of DEHP on dehydrogenase activity and microbial functional diversity in soil(DEHP对土壤脱氢酶活性及微生物功能多样性的影响).Acta pedologica sinica(土壤学报),2005,42(5):829-834.
[19]LIANG W,DENG J Q,ZHAN F C,et al.Effects of constructed wetland system on the removal of dibutyl phthalate(DBP).Microbiological Research,2009,164(2):206-211.
[20]XU X R,LI H B,GU J D.Biodegradation of an endocrinedisrupting chemical di-n-butyl phthalate ester by Pseudomonas fluorescens B-1.International Biodeterioration&Biodegradation,2005,55(1):9-15.
[21]HAN R(韩蕊),WANG D Y(王冬莹),RUI Y(芮洋),et al.Screen and property analysis of a fungus capable of degrading phthalate esters(一株降解邻苯二甲酸酯真菌的筛选及其降解特性研究).Environmental science(环境科学学报),2013,33(11):2941-2946.