香水莲花提取物对东莨菪碱诱导记忆障碍小鼠学习记忆能力的影响
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摘要
探究香水莲花提取物(Nymphaea hybrid extract,NHE)对东莨菪碱诱导记忆障碍小鼠的学习记忆能力的影响。采用腹腔注射东莨菪碱建立记忆障碍模型,Morris水迷宫实验测定小鼠空间学习和记忆能力。水迷宫实验结束后,断头处死小鼠,进行生化指标的测定。结果表明,与模型组小鼠相比,NHE干预后,小鼠的逃避潜伏期明显缩短(P <0. 01),目标象限停留时间百分比和穿越平台次数增加(P <0. 05或P <0. 01),小鼠海马和皮质区的SOD和GSH-PX活力显著升高(P <0. 01或P <0. 05),MDA含量极显著降低(P <0. 01),ACh E活性显著降低(P <0. 01),ACh含量增加(P <0. 01或P <0. 05)。同时,免疫印迹结果表明,NHE能够改善东莨菪碱引起小鼠海马和皮质中ERK、CREB磷酸化水平和BDNF蛋白表达的减少。综上,香水莲花提取物可以提高东莨菪碱诱导的记忆障碍小鼠的学习记忆能力,具体机制涉及缓解大脑的氧化应激损伤,平衡胆碱能系统,激活ERK-CREB-BDNF信号通路。
This research explored the effect of Nymphaea hybrid extract(NHE) on the learning and memory ability of scopolamine-induced memory impairment mice. The memory impairment model was established by intraperitoneal injection of scopolamine,and the spatial learning and memory ability of mice was determined by Morris water maze test. After the water maze experiment was completed,the mice were killed by decapitation and biochemical indicators were measured. The results showed that compared with the model group,after NHE intervention,the escape latency of the mice was significantly shortened(P <0. 01),the target quadrant residence time percentage and the number of crossing platforms were increased(P < 0. 05 or P <0. 01),the activity of SOD and GSH-PX in hippocampus and cortex of mice was significantly increased(P < 0. 01 or P < 0.05),the content of MDA was significantly decreased(P < 0. 01),and the activity of ACh E was significantly decreased(P <0. 01),ACh content was increased(P < 0. 01 or P < 0. 05). At the same time,the results of immunoblotting showed that NHE could ameliorate the decrease of the phosphorylated ERK and CREB,as well as BDNF protein expression in hippocampus and cortex of mice induced by scopolamine. In summary,NHE could enhance the learning and memory ability of scopolamine-induced memory impairment mice. The specific mechanism involved alleviating oxidative stress damage in the brain,balancing the cholinergic system,and activating the ERK-CREB-BDNF signaling pathway.
This research explored the effect of Nymphaea hybrid extract(NHE) on the learning and memory ability of scopolamine-induced memory impairment mice. The memory impairment model was established by intraperitoneal injection of scopolamine,and the spatial learning and memory ability of mice was determined by Morris water maze test. After the water maze experiment was completed,the mice were killed by decapitation and biochemical indicators were measured. The results showed that compared with the model group,after NHE intervention,the escape latency of the mice was significantly shortened(P <0. 01),the target quadrant residence time percentage and the number of crossing platforms were increased(P < 0. 05 or P <0. 01),the activity of SOD and GSH-PX in hippocampus and cortex of mice was significantly increased(P < 0. 01 or P < 0.05),the content of MDA was significantly decreased(P < 0. 01),and the activity of ACh E was significantly decreased(P <0. 01),ACh content was increased(P < 0. 01 or P < 0. 05). At the same time,the results of immunoblotting showed that NHE could ameliorate the decrease of the phosphorylated ERK and CREB,as well as BDNF protein expression in hippocampus and cortex of mice induced by scopolamine. In summary,NHE could enhance the learning and memory ability of scopolamine-induced memory impairment mice. The specific mechanism involved alleviating oxidative stress damage in the brain,balancing the cholinergic system,and activating the ERK-CREB-BDNF signaling pathway.
引文
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21 Zhang R,Xia ZM,Sun XY,et al.Role of ERK/CREB signal pathway in promotion of BDNF expression in Aβintoxicated SH-SY5Y cells by smilagenin[J].J Shanghai Jiao Tong Univ:Med Sci(上海交通大学学报:医学版),2016,36:642-647.
2 Budni J,Bellettini-Santos T,Mina F,et al.The involvement of BDNF,NGF and GDNF in aging and Alzheimer’s disease[J].Aging Dis,2015,6:331-341.
3 Min YU,Dong WL,Son HJ,et al.Phlorotannin-rich fraction from Ishige foliacea,brown seaweed prevents the scopolamine-induced memory impairment via regulation of ERK-CREB-BDNF pathway[J].J Fun Foods,2018,40:110-116.
4 Jin JH,Zhang WM.Acute and chronic toxicity of Nymphaea hybrida[J].Chin Wild Plant Res(中国野生植物资源),2015,34(1):5-9.
5 Shen JF,Che L,Hu XM,et al.Comparison of antioxidant activity from Nymphaea Hybrid of different color[J].J Chin Ins Food Sci Tech(中国食品学报),2013,13:214-218.
6 Jiang HF,Shi BJ,Zhao BT,et al.Separation and preparation of naringenin from Nymphaea hybrid by high-speed countercurrent chromatography[J].Chin Wild Plant Res(中国野生植物资源),2012,31(6):40-43.
7 Dong LQ.Study on the Regulation of lipid metabolism by Nymphaea hybrid extracts[D].Hangzhou:Zhejiang University(浙江大学),2017.
8 Zhang N,Hu Z,Zhang Z,et al.Protective role of naringenin against Aβ25-35-caused damage via ER and PI3K/Akt-Mediated pathways[J].Cell Mol Neurobiol,2017,1:1-9.
9 Youn K,Lee S,Jeong WS,et al.Protective role of corilagin on Aβ25-35-induced neurotoxicity:Suppression of NF-κB signaling pathway[J].J Med Food,2016,19:901-911.
10 Li XX,Wu XL,Jia SL,et al.Effect of chitosan oligosaccharide on learning and memory functions and serum antioxidant status in a rat model of Amyloid-β1-42-induced Alzheimer’s disease[J].Food Sci(食品科学),2017,38:220-225.
11 Wu XL,Li XX,Jia SL,et al.Protective effect and mechanism of fisetin on Aβ42-induced rat model of Alzheimer’s disease[J].Nat Prod Res Dev(天然产物研究与开发),2016,28:2011-2016.
12 Peng YR,Zhong FX,Yang B,et al.Effect of royal jelly on learning and memory performance of aged rats[J].Food Sci(食品科学),2011,32:269-272.
13 Ishola IO,Tota S,Adeyemi OO,et al.Protective effect of cnestis ferruginea and its active constituent on scopolamineinduced memory impairment in mice:a behavioral and biochemical study[J].Pharm Biol,2013,51:825-835.
14 Li J,Zhong ZL,Chu JR,et al.Effects of montelukast on learning memory and cholinergic system in model mice induced by scopolamine[J].Chin J Clin Pharm Thera(中国临床药理学与治疗学),2014,19:512-516.
15 Haider S,Tabassum S,Perveen T.Scopolamine-induced greater alterations in neurochemical profile and increased oxidative stress demonstrated a better model of dementia:A comparative study[J].Brain Res Bull,2016,127:234-247.
16 Cheignon C,Tomas M,Bonnefontrousselot D,et al.Oxidative stress and the amyloid beta peptide in Alzheimer’s disease[J].Redox Biol,2018,14:450-464.
17 Huang WJ,Zhang X,Chen WW.Role of oxidative stress in Alzheimer’s disease[J].Biomed Rep,2016,4:519-522.
18 Qin XY,Cao C,Cawley NX,et al.Decreased peripheral brain-derived neurotrophic factor levels in Alzheimer’s disease:a meta-analysis study(N=7277)[J].Mol Psychiatry,2017,22:312-320.
19 Tan L,Yang H,Pang W,et al.Investigation on the Role of BDNF in the benefits of Blueberry Extracts for the improvement of learning and memory in Alzheimer’s disease mouse model[J].J Alzheimers Dis,2017,56:629-640.
20 Li Y,Xu J,Xu P,et al.Xanthoceras sorbifolia extracts ameliorate dendritic spine deficiency and cognitive decline via upregulation of BDNF expression in a rat model of Alzheimer’s disease[J].Neurosci Lett,2016,629:208-214.
21 Zhang R,Xia ZM,Sun XY,et al.Role of ERK/CREB signal pathway in promotion of BDNF expression in Aβintoxicated SH-SY5Y cells by smilagenin[J].J Shanghai Jiao Tong Univ:Med Sci(上海交通大学学报:医学版),2016,36:642-647.