清宫寿桃丸对记忆障碍小鼠学习记忆的影响
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摘要
目的观察清宫寿桃丸对利血平、亚硝酸钠、乙醇诱导的记忆障碍小鼠学习记忆的影响。方法 ICR小鼠随机分为正常组、模型组、清宫寿桃丸(低、中、高剂量)组、金纳多组,每组14只。正常组和模型组给予等量的蒸馏水,清宫寿桃丸低、中、高剂量组分别按0.9、1.8、3.6 g/kg给药,金纳多组按0.12 g/kg给药,给药15天。干预14天后,获得性记忆障碍模型、巩固性记忆障碍模型、再现性记忆障碍模型分别使用利血平、亚硝酸钠及乙醇造模。干预15天后采用跳台实验及避暗实验评估学习记忆能力。结果与正常组比较,三种模型小鼠错误次数均明显增多,潜伏期均明显缩短(P<0.05);与模型组比较,清宫寿桃丸不同剂量均可减少三种模型小鼠的错误次数(P<0.05),其中高剂量还可延长获得性记忆障碍小鼠的潜伏期(P<0.05);另外金纳多可减少巩固性记忆障碍小鼠的错误次数(P<0.05)。结论清宫寿桃丸可改善获得性记忆障碍、巩固性记忆障碍和再现性记忆障碍,在改善记忆障碍方面的应用范围较广。
Objective To observe the effect of Qinggong Shoutao Pill on the dysmnesia models induced by reserpine, sodium nitrite and ethyl alcohol. Methods ICR mice were randomly divided into normal group, model group, Qinggong Shoutao Pill group(low, medium and high dose), and ginaton group, with 14 mice in each group. The normal and model groups were given equal amounts of distilled water, the low, medium and high dose of Qinggong Shoutao Pill groups were respectively given at the dose of 0.9 g/kg, 1.8 g/kg and 3.6 g/kg, and ginaton group was given at the dose of 0.12 g/kg. After drug treatment for 14 days, the dysmnesia models of memory acquisition, consolidation and reappearance were established by reserpine, sodium nitrite and ethyl alcohol respectively. The memory results were estimated by using Step-down Test and Dark-avoiding Test after 15-days-intervention. Results Compared with the normal group, the number of errors in three dysmnesia models was significantly increased, and the latent time was significantly shortened(P<0.05). Compared with the model groups, different dose of Qinggong Shoutao Pill could significantly reduce the number of errors(P<0.05), and the high dose of Qinggong Shoutao Pill could prolong the latent time in mice with acquired dysmnesia(P<0.05). In addition, ginaton could reduce the number of errors in mice with consolidated dysmnesia(P<0.05). Conclusion Qinggong Shoutao Pill could improve the memory acquirement, consolidation and reappearance, and has a more widely application in the treatment of dysmnesia.
Objective To observe the effect of Qinggong Shoutao Pill on the dysmnesia models induced by reserpine, sodium nitrite and ethyl alcohol. Methods ICR mice were randomly divided into normal group, model group, Qinggong Shoutao Pill group(low, medium and high dose), and ginaton group, with 14 mice in each group. The normal and model groups were given equal amounts of distilled water, the low, medium and high dose of Qinggong Shoutao Pill groups were respectively given at the dose of 0.9 g/kg, 1.8 g/kg and 3.6 g/kg, and ginaton group was given at the dose of 0.12 g/kg. After drug treatment for 14 days, the dysmnesia models of memory acquisition, consolidation and reappearance were established by reserpine, sodium nitrite and ethyl alcohol respectively. The memory results were estimated by using Step-down Test and Dark-avoiding Test after 15-days-intervention. Results Compared with the normal group, the number of errors in three dysmnesia models was significantly increased, and the latent time was significantly shortened(P<0.05). Compared with the model groups, different dose of Qinggong Shoutao Pill could significantly reduce the number of errors(P<0.05), and the high dose of Qinggong Shoutao Pill could prolong the latent time in mice with acquired dysmnesia(P<0.05). In addition, ginaton could reduce the number of errors in mice with consolidated dysmnesia(P<0.05). Conclusion Qinggong Shoutao Pill could improve the memory acquirement, consolidation and reappearance, and has a more widely application in the treatment of dysmnesia.
引文
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[2] 武力勇, 唐毅, 魏翠柏, 等. 防老年痴呆从中青年开始[N]. 健康报, 2017-9-2(4).
[3] 侯雪芹, 张磊, 林雅萍, 等.4种阿尔茨海默病动物模型行为学改变及凋亡机制的比较[J]. 中国老年学杂志, 2015, 35(12): 3185-3187.
[4] 韩玉梁, 贾建军, 吴卫平. 阿尔茨海默病的情景记忆障碍研究进展[J]. 中华老年心脑血管病杂志, 2017, 19(5): 555-558.
[5] Wilson RS, Boyle PA, Yu L, et al. Temporal course and pathologic basis of unawareness of memory loss in dementia[J]. Neurology, 2015, 85(11): 984-991.
[6] 罗燕, 陈真. 学习记忆障碍动物模型及行为学检测指标的评析[J]. 安徽医药, 2018, 22(2): 204-206.
[7] 徐立, 丛伟红, 魏翠娥, 等. 维脑康对小鼠记忆障碍的影响[J]. 中药药理与临床 2007, 23(6): 60-62.
[8] 李仪奎主编. 中药药理实验方法学[M]. 上海: 上海科学技术出版社, 1991: 171-172.
[9] 陈可冀, 周文泉, 李春生, 等. 清宫寿桃丸延缓衰老的临床及实验研究[J]. 中医杂志, 1985, 26(7): 25-28.
[10] 李春生. 清宫寿桃丸和御制平安丹的研究[N]. 中国中医药报, 2013-5-24(4).
[11] 吴红彦, 李海龙, 王虎平, 等. 大剂量当归对东莨菪碱致痴呆小鼠模型学习记忆及AchE、ChAT活性的影响[J]. 时珍国医国药, 2013, 24(3): 552-554.
[12] 李曦, 张丽宏, 王晓晓, 等. 当归化学成分及药理作用研究进展[J]. 中药材, 2013, 36(6): 1023-1028.
[13] 陈萍, 王培培, 焦泽沼, 等. 益智仁的化学成分及药理活性研究进展[J]. 现代药物与临床, 2013, 28(4): 617-623.
[14] 吴巧敏, 赵艺初, 韩艺凡, 等. 生酸枣仁、炒酸枣仁、酸枣果肉对PCPA失眠模型大鼠学习记忆能力影响的对比研究[J]. 中医药导报, 2016, 22(5): 72-75.
[15] 沈怡婷, 周颖, 陈霞, 等. 基于数据挖掘的中药改善学习记忆能力组方规律[J]. 中国老年学, 2017, 37(18): 4616-4619.
[16] EI Haj M, Antoine P, Nandrino JL, et al. Autobiographical memory decline in Alzheimer′s disease, a theoretical and clinical overview[J]. Ageing Res Rev, 2015, 23(Pt B): 183-192.