基于巯基-烯点击反应的PEG改性角蛋白膜的制备及其性能研究
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摘要
通过巯基-烯点击反应将亲水性聚合物聚乙二醇单甲醚甲基丙烯酸甲酯(MPEGMA)接枝到角蛋白大分子上,制备了PEG改性角蛋白,研究了其接枝反应条件及PEG改性角蛋白膜的性能。研究结果显示,MPEGMA成功接枝到角蛋白分子上,其较优反应条件为70℃、1h、引发剂浓度为5.5g/L、m(角蛋白)∶m(MPEGMA)=100:150,此时接枝率可以达到128%。经过PEG改性后的角蛋白溶液黏度显著变大,成膜性能得到提高,角蛋白膜的断裂强力随着接枝率的提高逐渐降低而断裂伸长率提高,细胞增殖实验显示,改性后的角蛋白膜材料对细胞无毒性,是良好的生物材料。
PEG-modified keratin was prepared by grafting the hydrophilic polymer polyethylene glycol monomethyl ether methyl methacrylate(MPEGMA)onto the keratin by the reaction of thiol-ene click chemistry.The graft reaction conditions and the properties of PEG-modified keratin film were studied in detail.The results showed that MPEGMA could be successfully grafted onto keratin molecules by thiol-ene click chemistry.The optimum reaction conditions were as follows:70℃,1 h,initiator concentration 5.5 g/L,keratin/MPEGMA=100 mg/150 mg,then GP can reach 128%.After PEG modification,the viscosity of keratin solution increased significantly and its film-forming property was improved.The breaking strength of film decreased with the increase of GP and the elongation at break increased.Cell proliferation bioassay showed that the film was non-toxic to the cells and could be used as a good biological material.
PEG-modified keratin was prepared by grafting the hydrophilic polymer polyethylene glycol monomethyl ether methyl methacrylate(MPEGMA)onto the keratin by the reaction of thiol-ene click chemistry.The graft reaction conditions and the properties of PEG-modified keratin film were studied in detail.The results showed that MPEGMA could be successfully grafted onto keratin molecules by thiol-ene click chemistry.The optimum reaction conditions were as follows:70℃,1 h,initiator concentration 5.5 g/L,keratin/MPEGMA=100 mg/150 mg,then GP can reach 128%.After PEG modification,the viscosity of keratin solution increased significantly and its film-forming property was improved.The breaking strength of film decreased with the increase of GP and the elongation at break increased.Cell proliferation bioassay showed that the film was non-toxic to the cells and could be used as a good biological material.
引文
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[18]陈钧辉,李俊.生物化学实验[M].北京:科学出版社,2014:58-60.
[2]Yamauchi K,Hojo H,Yamamoto Y,et al.Enhanced cell adhesion on RGDS-carrying keratin film[J].Materials Science&Engineering C,2003,23(4):467-472.
[3]Rouse J G,Dyke M E V.A review of keratin-based biomaterials for biomedical applications[J].Materials,2010,3(2):999-1014.
[4]陆声,杨海伟,李其训,等.人发角蛋白人工腱材料体外生物容性的实验研究[J].中国医师杂志,2005,7(6):759-761.
[5]陈英华,董为人,陈清元,等.复合生物敷料人发角蛋白-胶原海绵-聚甲基丙烯酸羟乙酯对大鼠烧伤创面的治疗作用[J].中国组织工程研究,2009,13(8):1432-1437.
[6]尹东,原林,赵卫东,等.人发角蛋白复合聚乳酸棒的力学强度测试及体内降解观察[J].南方医科大学学报,2003,23(5):442-444.
[7]Kakkar P,Verma S,Manjubala I,et al.Development of keratin-chitosan-gelatin composite scaffold for soft tissue engineering[J].Materials Science&Engineering C,2014,45:343-347.
[8]Silva R,Singh R,Sarker B,et al.Hybrid hydrogels based on keratin and alginate for tissue engineering[J].Journal of Materials Chemistry B,2014,2(33):5441-5451.
[9]张猛,马博谋,何春菊.离子液体法制备再生纤维素/角蛋白共混膜的研究[J].东华大学学报(自然科学版),2013,39(1):13-15.
[10]Vasconcelos A,Freddi G,Cavaco-Paulo A.Biodegradable materials based on silk fibroin and keratin[J].Biomacromolecules,2008,9(4):1299-1305.
[11]Sílvia Maria Martelli,Jo2o Borges Laurindo.Chicken feather keratin films plasticized with polyethylene glycol[J].International Journal of Polymeric Materials&Polymeric Biomaterials,2012,61(1):17-29.
[12]Wattie B,Dumont M J,Lefsrud M.Synthesis and properties of feather keratin-based superabsorbent hydrogels[J].Waste&Biomass Valorization,2018,9(3):391-400.
[13]袁久刚,王平,孙桂岩.羊毛角蛋白接枝聚丙烯酸-丙烯酰胺吸水树脂的制备[J].环境工程学报,2013,7(9):3695-3700.
[14]Martínezhernndez A L,Velascosantos C,Icaza M D,et al.Grafting of methyl methacrylate onto natural keratin[J].e-Polymers,2013,3(1):209-219.
[15]Shyam M G,Xiong Xiaobing,Lu Changhai,et al.Application of click chemistry in the preparation of poly(ethylene oxide)-block-poly(ε-caprolactone)with hydrolyzable cross-links in the micellar core[J].Macromolecules,2011,39(44):2058-2066.
[16]Luo K,Yang J,KopeˇckovP,et al.Biodegradable multiblock poly[N-(2-hydroxypropyl)methacrylamide]via reversible addition-fragmentation chain transfer polymerization and click chemistry[J].Macromolecules,2011,44(8):2481-2488.
[17]Li Q,Zhu L,Liu R,et al.Biological stimuli responsive drug carriers based on keratin for triggerable drug delivery[J].Journal of Materials Chemistry,2012,22(37):19964-19973.
[18]陈钧辉,李俊.生物化学实验[M].北京:科学出版社,2014:58-60.
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