两株嗜冷碳酸钙矿化菌对大理石表面修复效果研究
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摘要
细菌诱导的矿化沉积是一种有效修复和保护石质文物的方法。为探究生物矿化法应用于寒冷地区石质文物表面的修复效果,本文在黄龙高寒钙华沉积区分离到2株具高产碳酸酐酶(CA)活性的嗜冷型细菌菌株,并在大理石试件表面进行诱导矿化沉积。采用p H计、SEM、XRD等仪器,通过对矿化沉积过程中p H值、试件表面矿化沉积变化量、试件表面矿化沉积物的晶型晶貌、试件表面吸水性与透气性、耐酸度等的测定来表征嗜冷型碳酸酐酶产生菌在大理石试件表面矿化沉积的修复效果。结果表明,无论是存活菌还是灭活菌都在大理石试件表面生成了致密的碳酸钙矿化层,均为方解石晶型,形貌受菌体调控,活菌生成的矿化层更为均一和致密,长期作用比短期作用效果更明显,具有矿化层的试件在吸水性、透气性和耐酸度上都体现出更具防腐蚀的能力。其结果可为石质文物的修复提供一定的理论依据。
The mineralization induced by bacteria is an effective method for the restoration of stone relics.To explore the repair effect of biomineralization in low temperature region,2 psychrotrophic strains with high activity of carbonic anhydrase( CA) were isolated from cold water of Huanglong travertine deposition area. Calcium carbonat mineralization was induced by the 2 strains on the marble surface. The solution p H value, surface mineral deposition, surface morphology, surface water absorption, surface air permeability and surface acid resistance of marble during and after the mineralization process were analyzed by means of p H measure,SEM,XRD and other testing methods. The results were listed as following. Under the treatment with living bacteria or dead bacteria,the marble surface was covered with a dense calcified layer of calcite and the morphology was regulated by the bacteria. The living bacterial cells generated more homogenous and dense mineralized layer than dead cells. The remediation effect of long-term was more obvious than that of short-term. Compared with the blank specimen,the specimen with a mineralization layer show higher ability of corrosion prevention on water imbibitions, gas permeability and acid stability. The results would provide a theoretical basis for the restoration of stone relics under low temperature.
The mineralization induced by bacteria is an effective method for the restoration of stone relics.To explore the repair effect of biomineralization in low temperature region,2 psychrotrophic strains with high activity of carbonic anhydrase( CA) were isolated from cold water of Huanglong travertine deposition area. Calcium carbonat mineralization was induced by the 2 strains on the marble surface. The solution p H value, surface mineral deposition, surface morphology, surface water absorption, surface air permeability and surface acid resistance of marble during and after the mineralization process were analyzed by means of p H measure,SEM,XRD and other testing methods. The results were listed as following. Under the treatment with living bacteria or dead bacteria,the marble surface was covered with a dense calcified layer of calcite and the morphology was regulated by the bacteria. The living bacterial cells generated more homogenous and dense mineralized layer than dead cells. The remediation effect of long-term was more obvious than that of short-term. Compared with the blank specimen,the specimen with a mineralization layer show higher ability of corrosion prevention on water imbibitions, gas permeability and acid stability. The results would provide a theoretical basis for the restoration of stone relics under low temperature.
引文
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[13]Perito B,Biagiotti L,Daly S,et al.Bacterial genes involved in calcite crystal precipitation[D].Mcifferri O,Tisno P,Mastromei G.of Microbes and art-the role of microbial communities in the degradation and protect ion of cultural heritage[C].New York:Kluwer Academ icPlenum Publisher,2000:219-230.
[14]张文静,李琼芳,张存凯,等.嗜冷型产碳酸酐酶菌对碳酸钙沉积的影响[J].环境科学与技术.2016,39(3):1-11.
[15]Zhang W J,Li Q F,He X,et al.Psychrophilic carbonic anhydrase producing bacteria for screening and optimizing the enzyme production[J].Advanced Materials Research,2015,1092-1093:617-620.
[16]GB/T 9966.3-2001,天然饰面石材试验方法(第3部分):体积密度、真密度、真气孔率、吸水率试验方法[S].
[2]李沛豪,屈文俊,徐德强,等.大理石历史建筑遗产的细菌修复加固[J].华南理工大学学报:自然科学版,2009,37(9):36-41.
[3]李沛豪,屈文俊.生物修复加固石质文物研究进展[J].材料导报,2008,22(2):73-77.
[4]Rodriguez-Naarro C,Rodriguez-Gallego M,Ben Chekroun K,et al.Conservation of ornamental stone by myxococcus xanthus-induced carbonate biomineralization[J].Appl Envir Microbiol,2003,69(4):2182-219.
[5]孙延中,陈青.微生物技术文物保护中的应用研究述略[J].文物保护与考古科学,2008,20(3):68-72.
[6]Gaylarde C,Ribas Silva M,Warscheid Th.Microbial impacton buildingmaterials:an overview[J].Materials and Structures,2003,36(3):342-352.
[7]Rachael D Wakefield,Melanie S Jones.An introduction to stone colonizing micro-organisms and biodeterioration of building stone[J].Quarterly Journal of Engineering Geology and Hydrogeology,1998,31(4):301-313.
[8]Warscheid Th,Braams J.Biodeterioration of stone:a review[J].International Biodeterioration and Biodegradation,2000,46(4):343-368.
[9]Fernandes P.Applied microbiology and biotechnology in the conservation of stone cultural heritage materials[J].Applied Microbiology and Biotechnology,2006,73(2):291-296.
[10]Shen J Y,Cheng X H.Laboratory investigation on restoration of Chinese ancient masonry buildings using microbial carbonate precipitation[C]//In 1st Bio Geo Civil Engineering Conference.Delft,2008.
[11]Li W,Chen W S,Zhou P P,et al.Influence of initial calcium ion concentration on the precipitation and crystal morphology of calcium carbonate induced by bacterial carbonic anhydrase[J].Chemical Engineering Journal,2013,218:65-72.
[12]Dayana Arias,Luis A.Cisternas,Mariella Rivas.Biomineralization of calcium and magnesium crystals from seawater by halotolerant bacteria isolated from Atacama Salar(Chile)[J].Desalination,2017,405:1-9.
[13]Perito B,Biagiotti L,Daly S,et al.Bacterial genes involved in calcite crystal precipitation[D].Mcifferri O,Tisno P,Mastromei G.of Microbes and art-the role of microbial communities in the degradation and protect ion of cultural heritage[C].New York:Kluwer Academ icPlenum Publisher,2000:219-230.
[14]张文静,李琼芳,张存凯,等.嗜冷型产碳酸酐酶菌对碳酸钙沉积的影响[J].环境科学与技术.2016,39(3):1-11.
[15]Zhang W J,Li Q F,He X,et al.Psychrophilic carbonic anhydrase producing bacteria for screening and optimizing the enzyme production[J].Advanced Materials Research,2015,1092-1093:617-620.
[16]GB/T 9966.3-2001,天然饰面石材试验方法(第3部分):体积密度、真密度、真气孔率、吸水率试验方法[S].
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