施氮量对沙地羊草叶片非结构碳氮的影响
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摘要
在人工建植的沙地羊草草地设不同水平N肥(0、100、200、300、400 kg/hm~2)处理,分别用N0、N1、N2、N3、N4表示,研究不同N肥水平对沙地羊草叶片非结构碳氮的影响。结果表明:施氮可增加羊草叶片可溶性糖含量;随氮肥施入水平的增加,沙地羊草上部叶片的淀粉含量呈降低的变化趋势,第2茬沙地羊草下部叶片的淀粉含量呈先增加后降低的趋势;施N可显著增加沙地羊草叶片的可溶性蛋白含量(P<0.05),亦可增加第1茬羊草叶片的游离氨基酸含量;沙地羊草叶片C/N均随施氮的增加呈先降低后增加的变化趋势,上部叶片在N1水平下的C/N最小,试验得出N1(100 kg/hm~2)水平为该地区最佳施氮量。
Different levels of N fertilizer were applied to the sown Leymus chinensis pasture(0,N0;100,N1;200,N2;300,N3 and 400 kg/ha,N4) to study the effect of nitrogen application on unstructured carbon and nitrogen in leaf of Leymus chinensis in sandy land.The result showed that the nitrogen application increased the soluble sugar content.With the increase of nitrogen application level,the starch content in the upper leaf decreased,and it increased first and then decreased in the lower leaf in the second cut.Nitrogen application significantly increased the content of soluble protein(P<0.05),and the content of free amino acids in the first cut.The leaf C/N showed a trend of decreasing first and then increasing with the increase of nitrogen application level.The leaf C/N was the lowest under N1 treatment in upper leaf.It could be concluded that N1 treatment was the best.
Different levels of N fertilizer were applied to the sown Leymus chinensis pasture(0,N0;100,N1;200,N2;300,N3 and 400 kg/ha,N4) to study the effect of nitrogen application on unstructured carbon and nitrogen in leaf of Leymus chinensis in sandy land.The result showed that the nitrogen application increased the soluble sugar content.With the increase of nitrogen application level,the starch content in the upper leaf decreased,and it increased first and then decreased in the lower leaf in the second cut.Nitrogen application significantly increased the content of soluble protein(P<0.05),and the content of free amino acids in the first cut.The leaf C/N showed a trend of decreasing first and then increasing with the increase of nitrogen application level.The leaf C/N was the lowest under N1 treatment in upper leaf.It could be concluded that N1 treatment was the best.
引文
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[2] 刘兴元,龙瑞军,尚占环.草地生态系统服务功能及其价值评估方法研究[J].草业学报,2011,20(1):167-174.
[3] 李政海,王炜,刘钟龄.退化草原围封恢复过程中草场质量动态的研究[J].内蒙古大学学报(自然科学版),1995,26(3):334-327
[4] 梁燕,韩国栋,周禾,等.羊草草原退化程度判定的植物群落学指标[J].草地学报,2006,14(4):343-348.
[5] 王仁忠.放牧影响下羊草草地主要植物种群生态位宽度与生态位重叠的研究[J].植物生态学报,1997,21(4),304-311.
[6] 王玉辉,何兴元,周广胜.放牧强度对羊草草原的影响[J].草地学报,2002,10(1):45-49.
[7] 陈默君,贾慎修.中国饲料植物[M].北京:中国农业出版,2002:194-198.
[8] 李建东.我国的羊草草原[J].吉林师范大学学报(自然科学版),1979(3):145-151.
[9] 郭本兆.中国植物志[M].北京:科学出版社,1987.
[10] 陈佐忠,汪诗平.中国典型草原生态系统[M].北京:中国科学技术出版社,2000.
[11] 祝廷成.羊草生物生态学[M].长春:吉林科学技术出版社,2004.
[12] WANG Y,ZHOU G,WANG Y.Modeling responses of the meadow steppe dominated by Leymus chinensis to climate change[J].Climatic Change,2007,82:437-452.
[13] K?RNER C.Carbon limitation in trees[J].Journal of Ecology,2003,91(1):4-17.
[14] LI M H,XIAO W F,WANG S G,et al.Mobile carbohydrates in Himalayan treeline trees I.Evidence for carbon gain limitation but not for growth limitation[J].Tree Physiology,2008,28(8):1287-1296.
[15] YIN J J,GUO D L,HE S Y,et al.Non-structural carbohydrates,N,and P allocation patterns of two temperate tree species in a semi-arid region of Inner Mongolia[J].Acta Scientiarum Naturalium Universitatis Pekinensis,2009,45(3):519-527.
[16] 李淑文,文宏达,薛宝民,等.小麦高效吸收利用氮素的生理生化特性研究进展[J].麦类作物学报,2003,23(4):131-135.
[17] 路文静,张树华,郭程瑾,等.不同氮素利用效率小麦品种的氮效率相关生理参数研究[J].植物营养与肥料学报,2009,15(5):985-991.
[18] 郝再彬,苍品,徐仲.生理学实验[M].哈尔滨:哈尔滨工业大学出版社,2004.
[19] 高俊凤.植物生理实验技术[M].广州:世界图书出版社,2000.
[20] 邹琦.植物生理学实验指导[M].北京:中国农业出版社,2000.
[21] 李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[22] 朱爱民,张玉霞,王显国,等.秋末刈割处理对沙地苜蓿冬季根颈非结构碳氮的影响[J].草业学报,2018,27(1):86-96.
[23] 吕中显,赵铭钦,赵进恒,等.烤烟打顶后不同部位烟叶碳氮代谢关键酶活性的动态变化及相关分析[J].江西农业大学学报,2010,32(4):700-704.
[24] MAKINO A,MAE T,OHIRA K.Changes in Photosynthetic capacity in rice leaves from emergence through senescence.Analysis from ribulose-1,5-bisPhosPhata carboxylase and leaf conductance[J].Plant and Cell Physiology,1984,25(3):511-521.