25-羟维生素D_3水平对ITP患儿外周血Th17/Treg相关细胞因子表达的影响
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摘要
目的探讨血清25-羟维生素D_3水平对特发性血小板减少性紫癜(ITP)患儿外周血中Th17/Treg相关细胞因子的表达水平影响。方法收集九江学院附属医院及九江市妇女儿童医院ITP患儿32例,测定血清25-羟维生素D_3浓度,依据25-羟维生素D_3浓度分为缺乏组(低于20ng/mL),不足组(20～30ng/mL)及正常组。同时以30名健康儿童为对照组,分别检测各组血清IL-17、IL-21、IL-10及TGF-β的表达水平。结果 ITP患儿组与正常组的年龄(t=1.989,P=0.682)、性别(χ~2=1.013,P=0.859)无统计学意义。ITP患儿中维生素D_3缺乏组和不足组的IL-10及TGF-β表达水平明显低于正常组和健康对照组(P<0.05),而IL-17及IL-21水平则显著高于ITP患儿的维生素D_3正常组和健康对照组(P<0.05)。结论血清25-羟维生素D_3缺乏或减少与IL-17、IL-21、IL-10、TGF-β的表达水平密切相关,并对ITP患儿外周血Th17/Treg相关细胞因子表达起重要的调节作用。
Objective To investigate the effect of serum25-hydroxyvitamin D_3 level on the expression of Th17/Treg-related cytokines in peripheral blood of children with idiopathic thrombocytopenic purpura(ITP).Methods 32 children with ITP were collected from the affiliated Hospital of Jiujiang University and Jiujiang Women and Children Hospital.The serum concentrations of 25-hydroxyvitamin D_3 were measured and divided into deficiency group(lower than 20 ng/mL)according to the concentration of 25-hydroxyvitamin D_3.Deficiency group(between 20-30 ng/mL)and normal group.At the same time,30 healthy children were used as control,and serum I was detected in each group.The expression levels of L-17,IL-21,IL-10 and TGF-βwere found.Results The age and sex of ITP group and normal group(t=1.989,P=0.682),(χ~2=1.013,respectively),the age of children and normal group(t=1.989,P=0.682),sex(χ~2=1.013),The expression levels of IL-10 and TGF-βin vitamin D_3 deficiency group and deficiency group were significantly lower than those in normal group and healthy control group.The levels of IL-17 and IL-21 in ITP children were significantly higher than those in normal vitamin D_3 group and healthy control group(P<0.05).Conclusion The deficiency or decrease of serum25-hydroxyvitamin D_3 is closely related to the expression of IL-17,IL-21,IL-10,TGF-β,and plays an important role in regulating the expression of Th17/Treg related cytokines in peripheral blood of children with ITP.
Objective To investigate the effect of serum25-hydroxyvitamin D_3 level on the expression of Th17/Treg-related cytokines in peripheral blood of children with idiopathic thrombocytopenic purpura(ITP).Methods 32 children with ITP were collected from the affiliated Hospital of Jiujiang University and Jiujiang Women and Children Hospital.The serum concentrations of 25-hydroxyvitamin D_3 were measured and divided into deficiency group(lower than 20 ng/mL)according to the concentration of 25-hydroxyvitamin D_3.Deficiency group(between 20-30 ng/mL)and normal group.At the same time,30 healthy children were used as control,and serum I was detected in each group.The expression levels of L-17,IL-21,IL-10 and TGF-βwere found.Results The age and sex of ITP group and normal group(t=1.989,P=0.682),(χ~2=1.013,respectively),the age of children and normal group(t=1.989,P=0.682),sex(χ~2=1.013),The expression levels of IL-10 and TGF-βin vitamin D_3 deficiency group and deficiency group were significantly lower than those in normal group and healthy control group.The levels of IL-17 and IL-21 in ITP children were significantly higher than those in normal vitamin D_3 group and healthy control group(P<0.05).Conclusion The deficiency or decrease of serum25-hydroxyvitamin D_3 is closely related to the expression of IL-17,IL-21,IL-10,TGF-β,and plays an important role in regulating the expression of Th17/Treg related cytokines in peripheral blood of children with ITP.
引文
[1]王君霞.儿童特发性血小板减少性紫癜诊治进展[J].西北国防医学杂志,2016,37(8):538-541.
[2]Berendsen AA,Lieshout LE,Eg VDH,et al.Conventional foods,followed by dietary supplements and fortified foods,are the key sources of vitamin D,vitamin B6,and selenium intake in Dutch participants of the NU-AGE study[J].Nutrition Research,2016,36(10):1171-1181.
[3]白峰岩,李戈,王刚,等.特发性血小板减少性紫癜患儿白介素与细胞免疫的变化研究[J].中国现代医学杂志,2016,26(13):75-78.
[4]张之南.血液病诊断与疗效标准[M].第3版.北京:科学出版社,2008:172-175.
[5]George JN.The importance of clinical judgment for the diagnosis of thrombotic thrombocytopenic purpura[J].Transfusion,2017,57(11):2558-2561.
[6]王颖超,刘满菊,朱桂英,等.Th17/Treg细胞比例失衡在儿童原发性免疫性血小板减少症中的意义[J].中国当代儿科杂志,2016,18(3):238-242.
[7]Liu X,Hu H,Fan H,et al.The role of STAT3 and AhR in the differentiation of CD4+T cells into Th17 and Treg cells[J].Medicine,2017,96(17):e6615-e6618.
[8]Mahabady S,Tjokro N,Aharonian S,et al.The in vivo Th17 and Treg immune responses to Aggregatibacter actinomycetemcomitans[J].Molecular Oral Microbiology,2017,32(6):490-499.
[9]Yu S,Liu C,Li L,et al.Inactivation of Notch signaling reverses the Th17/Treg imbalance in cells from patients with immune thrombocytopenia[J].Lab Invest.2015,95(2):157-167.
[10]冯媛,耿玲玲,李小青,等.MicroRNA-15a在儿童原发性免疫性血小板减少症中的表达及其意义[J].中国实验血液学杂志,2017,25(6):1772-1775.
[11]Wang S,Guan Y,Wang Y,et al.Reduced PTEN involved in primary immune thrombocytopenia via contributing to B cell hyper-responsiveness[J].Molecular Immunology,2018,93:144-151.
[12]Ryu JG,Lee J,Kim EK,et al.Treatment of IL-21R-Fc control autoimmune arthritis via suppression of STAT3 signal pathway mediated regulation of the Th17/Treg balance and plasma Bcells[J].Immunol Lett,2015,163(2):143-150.
[13]Liu X,Hu H,Fan H,et al.The role of STAT3 and AhR in the differentiation of CD4+T cells into Th17 and Treg cells[J].Medicine,2017,96(17):e6615-e6619.
[14]Gautron AS,Dominguez-Villar M,De MM,et al.Enhanced suppressor function of TIM-3+FoxP3+regulatory T cells[J].Eur J of Im,2015,44(9):2703-2711.
[15]Talaat RM,Elmaghraby AM,Barakat SS,et al.Alterations in immune cell subsets and their cytokine secretion profile in childhood idiopathic thrombocytopenic purpura(ITP)[J].Clin Exp Im,2014,176(2):291-300.
[16]顾磊,徐庆,曹晖.1,25-二羟基维生素D3对Th17细胞调控的研究进展[J].实用医学杂志,2015,31(24):4141-4142.
[17]温鸿雁,罗静,张晓英,等.1,25-二羟维生素D3对初诊类风湿关节炎患者外周血单个核细胞分泌Th17相关细胞因子的影响[J].中华内科杂志,2015,54(4):317-321.
[18]Zhang H,Wu H,Liu L,et al.1,25-dihydroxyvitamin D3regulates the development of chronic colitis by modulating both Thelper(Th)1 and Th17 activation[J].Apmis,2015,123(6):490-501.
[19]Fawaz L,Mrad MF,Kazan JM,et al.Comparative effect of25(OH)D3and 1,25(OH)2D3on Th17 cell differentiation[J].Clinical Im,2016,166(1):59-71.
[20]Zhou Q,Qin S,Zhang J,et al.1,25(OH)2D3induces regulatory T cell differentiation by influencing the VDR/PLC-γ1/TGF-β1/pathway.[J].Mol Im,2017,91:156-164.
[2]Berendsen AA,Lieshout LE,Eg VDH,et al.Conventional foods,followed by dietary supplements and fortified foods,are the key sources of vitamin D,vitamin B6,and selenium intake in Dutch participants of the NU-AGE study[J].Nutrition Research,2016,36(10):1171-1181.
[3]白峰岩,李戈,王刚,等.特发性血小板减少性紫癜患儿白介素与细胞免疫的变化研究[J].中国现代医学杂志,2016,26(13):75-78.
[4]张之南.血液病诊断与疗效标准[M].第3版.北京:科学出版社,2008:172-175.
[5]George JN.The importance of clinical judgment for the diagnosis of thrombotic thrombocytopenic purpura[J].Transfusion,2017,57(11):2558-2561.
[6]王颖超,刘满菊,朱桂英,等.Th17/Treg细胞比例失衡在儿童原发性免疫性血小板减少症中的意义[J].中国当代儿科杂志,2016,18(3):238-242.
[7]Liu X,Hu H,Fan H,et al.The role of STAT3 and AhR in the differentiation of CD4+T cells into Th17 and Treg cells[J].Medicine,2017,96(17):e6615-e6618.
[8]Mahabady S,Tjokro N,Aharonian S,et al.The in vivo Th17 and Treg immune responses to Aggregatibacter actinomycetemcomitans[J].Molecular Oral Microbiology,2017,32(6):490-499.
[9]Yu S,Liu C,Li L,et al.Inactivation of Notch signaling reverses the Th17/Treg imbalance in cells from patients with immune thrombocytopenia[J].Lab Invest.2015,95(2):157-167.
[10]冯媛,耿玲玲,李小青,等.MicroRNA-15a在儿童原发性免疫性血小板减少症中的表达及其意义[J].中国实验血液学杂志,2017,25(6):1772-1775.
[11]Wang S,Guan Y,Wang Y,et al.Reduced PTEN involved in primary immune thrombocytopenia via contributing to B cell hyper-responsiveness[J].Molecular Immunology,2018,93:144-151.
[12]Ryu JG,Lee J,Kim EK,et al.Treatment of IL-21R-Fc control autoimmune arthritis via suppression of STAT3 signal pathway mediated regulation of the Th17/Treg balance and plasma Bcells[J].Immunol Lett,2015,163(2):143-150.
[13]Liu X,Hu H,Fan H,et al.The role of STAT3 and AhR in the differentiation of CD4+T cells into Th17 and Treg cells[J].Medicine,2017,96(17):e6615-e6619.
[14]Gautron AS,Dominguez-Villar M,De MM,et al.Enhanced suppressor function of TIM-3+FoxP3+regulatory T cells[J].Eur J of Im,2015,44(9):2703-2711.
[15]Talaat RM,Elmaghraby AM,Barakat SS,et al.Alterations in immune cell subsets and their cytokine secretion profile in childhood idiopathic thrombocytopenic purpura(ITP)[J].Clin Exp Im,2014,176(2):291-300.
[16]顾磊,徐庆,曹晖.1,25-二羟基维生素D3对Th17细胞调控的研究进展[J].实用医学杂志,2015,31(24):4141-4142.
[17]温鸿雁,罗静,张晓英,等.1,25-二羟维生素D3对初诊类风湿关节炎患者外周血单个核细胞分泌Th17相关细胞因子的影响[J].中华内科杂志,2015,54(4):317-321.
[18]Zhang H,Wu H,Liu L,et al.1,25-dihydroxyvitamin D3regulates the development of chronic colitis by modulating both Thelper(Th)1 and Th17 activation[J].Apmis,2015,123(6):490-501.
[19]Fawaz L,Mrad MF,Kazan JM,et al.Comparative effect of25(OH)D3and 1,25(OH)2D3on Th17 cell differentiation[J].Clinical Im,2016,166(1):59-71.
[20]Zhou Q,Qin S,Zhang J,et al.1,25(OH)2D3induces regulatory T cell differentiation by influencing the VDR/PLC-γ1/TGF-β1/pathway.[J].Mol Im,2017,91:156-164.