P(St-GMA)-IDA-Cu(Ⅱ)微球的制备及其吸附性能
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摘要
采用分散聚合法制备聚P(St-GMA)微球,通过开环反应将亚氨基二乙酸(iminodiacetic acid,IDA)接枝在微球的表面,然后与金属离子的螯合,制备了一种P(St-GMA)-IDA-Cu(Ⅱ)微球吸附剂。采用扫描电镜(SEM)、红外光谱(IR)和电导率仪等对其进行了表征。探讨了P(St-GMA)-IDA-Cu(Ⅱ)微球对牛血清蛋白(bovine serum albumin,BSA)的吸附性能,考察了pH值和吸附时间等对BSA的吸附量的影响,研究了其吸附动力学和吸附热力学特性。结果表明,pH=6时,吸附量最大;在298K时,P(St-GMA)-IDA-Cu(Ⅱ)微球的最大吸附量为37.66mg/g,吸附符合Langmuir方程;动力学研究结果表明,准二级动力学方程能较好拟合动力学实验结果。
P(Styrene-Glycidyl methacrylate)(P(St-GMA))microspheres were synthesized adopting dispersion copolymerization,and iminodiacetic acid(IDA)was then grafted on the surface of P(St-GMA)microspheres via the ring-opening reaction,to form P(St-GMA)-IDA microspheres.New P(St-GMA)-IDA-Cu(Ⅱ)microspheres were prepared by coordinating Cu(Ⅱ)on P(St-GMA)-IDA microspheres.The adsorbent was characterized by scanning electron microscope(SEM),Fourier transform infrared spectrometer(FT-IR),and conductivity meter.The adsorption performances of bovine serum albumin(BSA)on P(St-GMA)-IDA-Cu(Ⅱ)microspheres was discussed in detail,including pH and contact time.The thermodynamic and kinetic parameters in adsorption process were studied to discuss the mechanism of adsorption.The experimental results showed that the highest adsorption capacity of P(St-GMA)-IDA-Cu(Ⅱ)microspheres was around pH=6.The highest adsorption capacity was 37.66 mg/g at 298 Kand the equilibrium adsorption data fitted well to Langmuir.The kinetic experimental data were correlated with second-order kinetic model.
P(Styrene-Glycidyl methacrylate)(P(St-GMA))microspheres were synthesized adopting dispersion copolymerization,and iminodiacetic acid(IDA)was then grafted on the surface of P(St-GMA)microspheres via the ring-opening reaction,to form P(St-GMA)-IDA microspheres.New P(St-GMA)-IDA-Cu(Ⅱ)microspheres were prepared by coordinating Cu(Ⅱ)on P(St-GMA)-IDA microspheres.The adsorbent was characterized by scanning electron microscope(SEM),Fourier transform infrared spectrometer(FT-IR),and conductivity meter.The adsorption performances of bovine serum albumin(BSA)on P(St-GMA)-IDA-Cu(Ⅱ)microspheres was discussed in detail,including pH and contact time.The thermodynamic and kinetic parameters in adsorption process were studied to discuss the mechanism of adsorption.The experimental results showed that the highest adsorption capacity of P(St-GMA)-IDA-Cu(Ⅱ)microspheres was around pH=6.The highest adsorption capacity was 37.66 mg/g at 298 Kand the equilibrium adsorption data fitted well to Langmuir.The kinetic experimental data were correlated with second-order kinetic model.
引文
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[16]Yan H,Li H J,Yang H,et al.Removal of various cationic dyes from aqueous solutions using a kind of fully biodegradable magnetic composite microsphere[J].Chemical Engineering Journal,2013,223(5):402-411.
[17]Wang Z L,Yue T L,Yuan Y H,et al.Kinetics of adsorption of bovine serum albumin on magnetic carboxymethyl chitosan nanoparticles[J].International Journal of Biological Macromolecules,2013,58(7):57-65.
[18]Cheng Yue,Xiao Zhiguo,Yu Hongwei,et al.Preparation and adsorption properties of amino modified mesoporous microsphere Fe3O4@SiO2@mSiO2 magnetic adsorbent[J].Journal of Functional Materials,2017,48(12):12135-12141(in Chinese).成岳,肖治国,余宏伟,等.氨基化修饰介孔Fe3O4@SiO2@mSiO2磁性吸附剂的制备及吸附性能的研究[J].功能材料,2017,48(12):12135-12141.
[19]Ke F,Jiang J,Li Y Z,et al.Highly selective removal of Hg2+and Pb2+by thiol-functionalized Fe3O4@metal-organic framework core-shell magnetic microspheres[J].Applied Surface Science,2017,413(8):266-274.
[2]Chai M,Ye Y,Chen V.Separation and concentration of milk proteins with a submerged membrane vibrational system[J].Journal of Membrane Science,2017,524(2):305-314.
[3]Zhang Y M,Wang W T,Ma X D,et al.Polydopamine assisted fabrication of titanium oxide nanoparticles modified column for proteins separation by capillary electrochromatography[J].Analytical Biochemistry,2016,512(11):103-109.
[4]Cao Miao,Li Zhonghong,Zhu Wei.Adsorption of bovine serum albumin by reactive red 120modified magnetic chitosan microspheres[J].Food Science,2013,34(21):64-68(in Chinese).曹淼,李忠宏,朱伟.活性红120改性磁性壳聚糖微球对牛血清白蛋白的吸附性能[J].食品科学,2013,34(21):64-68.
[5]Ding Libin,Wang Yifeng.The preparation and characterization of protein imprinting polymer of chitosan and metal ion coordination compound[J].Polymer Materials Science and Engineering,2010,26(02):125-127(in Chinese).丁利斌,王艺峰.壳聚糖金属离子配合物蛋白质印迹聚合物的制备与表征[J].高分子材料科学与工程,2010,26(02):125-127.
[6]Kara A,Osman B,Yavuz H,et al.Immobilization ofα-amylase on Cu2+chelated poly(ethylene glycol dimethacrylate nvinylimidazole)matrix via adsorption[J].Reactive and Functional Polymers,2005,62(5):61-68.
[7]Chen J X,Zhao C,Huang H H,et al.Highly crosslinked poly(ethyleneglycol dimethacrylate)-based microspheres via solvothermal precipitation polymerization in alcohol water system[J].Polymer,2015,83(1):214-222.
[8]Liu X Y,Zheng S W,Hong R Y,et al.Preparation of magnetic poly(styrene-co-acrylic acid)microspheres with adsorption of protein[J].Colloids and Surfaces A:Physicochemical and Engineering Aspects.2014,443(2):425-431.DOI:10.1016/j.colsurfa.2013.11.025.
[9]Tian Q,Yu D M,Zhu K M,et al.Multi-hollow polymer microspheres with enclosed surfaces and compartmentalized voids prepared by seeded swelling polymerization method[J].Journal of Colloid and Interface Science,2016,473(7):44-51.DOI:10.1016/j.jcis.2016.03.063.
[10]Chen J X,Zhao C,Huang H H,et al.Highly crosslinked poly(ethyleneglycol dimethacrylate)-based microspheres via solvothermal precipitation polymerization in alcohol water system[J].Polymer,2015,83(1):214-222.DOI:10.1016/j.polymer.2015.12.028.
[11]Ye Qing,Wang Shibing,Ye Wenyu.Preparation of novel immobilized metal ion affinity adsorbent for protein adsorption[J].Journal of Henan University(Natural Science),2007,37(2):152-155(in Chinese).叶青,王守斌,叶文玉.新型固定金属离子亲和吸附剂的制备及对蛋白质的吸附研究[J].河南大学学报(自然科学版),2007,37(2):152-155.
[12]Shi Ruobing,Zhang Zhichao,Chen Lei,et al.Magnetic silica microspheres as a novel support for immobilized metal affinity purification of proteins/peptides[J].Chinese Journal of Analytical Chemistry,2007,35(5):628-632(in Chinese).石若冰,张志超,陈磊,等.基于磁性微球的固定化金属亲和载体及其在蛋白质/多肽纯化中的应用[J].分析化学,2007,35(5):628-632.
[13]Wang K,Qiu G M,Cao H Y,et al.Removal of chromium(Ⅵ)from aqueous solutions using Fe3O4 magnetic polymer microspheres functionalized with amino groups[J].Materials,2015,8:8378-8391.
[14]Hwang C W,Kwak N S,Hwang T S.Preparation of poly(GMA-co-PEGDA)microbeads modified withiminodiacetic acid and their indium adsorption properties[J].Chemical Engineering Journal,2013,226:79-86.
[15]Li Rong,Chen Guoliang,Zhao Wenming.Immobilized metal ion affinity chromatography—methods,principles,characteristics and applications for protein separation[J].Chemistry Bulletin,2005,5:352-360(in Chinese).李蓉,陈国亮,赵文明.固定金属离子亲和色谱—蛋白质分离的方法、原理、特性和应用[J].化学通报,2005,5:352-360.
[16]Yan H,Li H J,Yang H,et al.Removal of various cationic dyes from aqueous solutions using a kind of fully biodegradable magnetic composite microsphere[J].Chemical Engineering Journal,2013,223(5):402-411.
[17]Wang Z L,Yue T L,Yuan Y H,et al.Kinetics of adsorption of bovine serum albumin on magnetic carboxymethyl chitosan nanoparticles[J].International Journal of Biological Macromolecules,2013,58(7):57-65.
[18]Cheng Yue,Xiao Zhiguo,Yu Hongwei,et al.Preparation and adsorption properties of amino modified mesoporous microsphere Fe3O4@SiO2@mSiO2 magnetic adsorbent[J].Journal of Functional Materials,2017,48(12):12135-12141(in Chinese).成岳,肖治国,余宏伟,等.氨基化修饰介孔Fe3O4@SiO2@mSiO2磁性吸附剂的制备及吸附性能的研究[J].功能材料,2017,48(12):12135-12141.
[19]Ke F,Jiang J,Li Y Z,et al.Highly selective removal of Hg2+and Pb2+by thiol-functionalized Fe3O4@metal-organic framework core-shell magnetic microspheres[J].Applied Surface Science,2017,413(8):266-274.