多肉植物姬玉露组织培养体系的建立
|
摘要
姬玉露是玉露最为常见的品种之一。本试验以姬玉露新生肉质叶为外植体,探索最佳外植体消毒方式,并研究不同激素配比对诱导姬玉露愈伤组织及其分化的影响,建立姬玉露组织培养快速繁殖体系。结果表明:以2%的次氯酸钠为消毒剂最佳消毒时间为9 min;诱导姬玉露愈伤组织的最佳培养基配方为MS+6-BA (1.0 mg/L)+2,4-D (0.3 mg/L);诱导姬玉露愈伤组织分化的最佳培养基配方为MS+6-BA (1.0 mg/L)+NAA (0.1 mg/L);诱导姬玉露生根的最佳培养基为1/2MS+IBA (0.6 mg/L),生根率能达到76.3%。再生植株移栽最佳的基质为草炭土:蛭石:珍珠岩=3:2:1,移栽成活率达100%。本试验通过组织培养的方法,解决姬玉露繁殖率低、生长慢、品质差等问题,从而为工厂化育苗提供技术支持。
Haworthia cooperi var. truncate is a one of the most common variety of Haworthia cooperi. With the leaf of Haworthia cooperi var. truncate, to find the optimal disinfection time of explants, and study the influence of the different ratio of hormones on the callus induction and regeneration, and a high frequency in vitro regeneration system was established. The results showed that: with the 2% Na ClO and disinfecting 9 min has the best effect.The optimal medium for inducing the callus of Haworthia cooperi var. truncate is MS+6-BA(1.0 mg/L)+2,4-D(0.3 mg/L);The optimum medium of adventitious bud introduction is MS+6-BA(1.0 mg/L)+NAA(0.1 mg/L). The optimal medium for inducing the rooting of Haworthia cooperi var. truncate is 1/2 MS+IBA(0.6 mg/L), and the rooting rate is up to 76.3%. The best substratum for regenerated plant transplantation is peat: vermiculite:perlite =3:2:1, and the graft survival rate reached 100%. This experiment solved the problems of low reproduction rate,slow growth and poor quality of Haworthia cooperi var. truncate, so as to provide technical support for factory seed breeding.
Haworthia cooperi var. truncate is a one of the most common variety of Haworthia cooperi. With the leaf of Haworthia cooperi var. truncate, to find the optimal disinfection time of explants, and study the influence of the different ratio of hormones on the callus induction and regeneration, and a high frequency in vitro regeneration system was established. The results showed that: with the 2% Na ClO and disinfecting 9 min has the best effect.The optimal medium for inducing the callus of Haworthia cooperi var. truncate is MS+6-BA(1.0 mg/L)+2,4-D(0.3 mg/L);The optimum medium of adventitious bud introduction is MS+6-BA(1.0 mg/L)+NAA(0.1 mg/L). The optimal medium for inducing the rooting of Haworthia cooperi var. truncate is 1/2 MS+IBA(0.6 mg/L), and the rooting rate is up to 76.3%. The best substratum for regenerated plant transplantation is peat: vermiculite:perlite =3:2:1, and the graft survival rate reached 100%. This experiment solved the problems of low reproduction rate,slow growth and poor quality of Haworthia cooperi var. truncate, so as to provide technical support for factory seed breeding.
引文
Bai Y.E.,Peng P.,Duan G.Z.,Ming J.,Cheng A.J.,and Niu X.Y.,2017,Tissue culture and regenerated of the different ex-plants of Aster novibelgii,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),15(5):1887-1893(白玉娥,彭鹏,段国珍,明健,陈嫒婕,牛新月,2017,荷兰菊不同外植体组织培养快繁体系,分子植物育种,15(5):1887-1893)
Liu F.,Tang Y.H.,Yuan Y.M.,Guo Q.Q.,Shen F.,and Chen J.R.,2016,Tissue culture of the succulent plant Sedum clavatum,Zhiwu Xuebao(Chinese Bulletin of Botany),51(2):251-256(刘芳,唐映红,袁有美,郭清泉,沈帆,陈建荣,2016,多肉植物劳尔的组织培养,植物学报,51(2):251-256)
She Q.H.,Jiang Y.Y.,Yang C.,Gou L.Q.,Zhu B.Y.,and Zhang L.,2018,Research progress on tissue culture and cultivation of plants from Hemsleya Cogn.,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),37(3):1370-1375(佘启惠,姜媛媛,杨程,苟丽琼,朱柏雨,张利,2018,雪胆属植物的组织培养与栽培研究进展,基因组学与应用生物学,37(3):1370-1375)
Tuo Y.,and Bai Y.E.,2018,Callus induction and plant regeneration by shoot tip of Ribes odoratum Wendl,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),16(7):2255-2259(托亚,白玉娥,2018,香茶藨子茎尖愈伤组织诱导及植株再生,分子植物育种,16(7):2255-2259)
Wang Q.,Zuo Z.Y.,Song X.T.,Zhang Y.,Zhang H.P.,Yin X.S.,An X.Y.,Yang X.,and Sun T.,2008,Tissue culture and rapid propagation of Haworthia emelyae var.major(G.G.Smith)M.B.Bayer,Zhiwu Shengli Xuebao(Plant Physiology Journal),44(1):123-124(王泉,左志宇,宋晓涛,张耀,张昊鹏,尹晓爽,安晓云,杨雪,孙涛,2008,百合科多肉植物美吉寿的组织培养与快速繁殖,植物生理学报,44(1):123-124)
Wang T.,2018,Study on rapid propagation technology system with tissue culture of Haworthia cooperi var.truncate,Thesis for M.S.,Gansu Agricultural University,Supervisor:Ma Y.J.,pp.31-56(王婷,2018,姬玉露组织培养快繁技术体系研究,硕士学位论文,甘肃农业大学,导师:马彦军,pp.31-56)
Wang Y.,Xu G.,Wang Y.T.,Mou H.J.,LüY.P.,Chen Z.,and Chen J.P.,2013,Study on the tissue culture and germplasm innovation of Gasteria armstrongi verieg.,Zhejiang Nongye Xuebao(Acta Agriculturae Zhejiangensis),25(3):488-491(王燕,徐刚,汪一婷,牟豪杰,吕永平,陈志,陈剑平,2013,卧牛锦的组织培养及其种质创新初探,浙江农业学报,25(3):488-491)
Wang Z.S.,Wang G.D.,and Wang Y.,2014,Study on in vitro culture and rapid propagation in Haworthia emelyae var.emelyae V.poelln'Miracle',Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),33(6):1329-1335(王紫珊,王广东,王雁,2014,多肉植物白银寿‘奇迹’的离体培养与快速繁殖,基因组学与应用生物学,33(6):1329-1335)
Wu Z.J.,Huang X.J.,Wang B.,Peng T.,and Ma W.Y.,2015,Tissue culture and rapid propagation of Ophthalmophyllum friedrichiae,Zhiwu Shengli Xuebao(Plant Physiology Journal),51(11):2013-2016(吴正景,黄雪娇,王柏,彭涛,马文瑶,2015,风铃玉的组织培养与快速繁殖,植物生理学报,51(11):2013-2016)
Zhang H.P.,Song X.T.,Zhang Y.,Yu M.R.,Tian L.,Li H.Y.,Zuo Z.Y.,An X.Y.,Yang X.,and Sun T.,2008,Tissue culture and rapid propagation of Haworthia emelyae var.emelyae V.poelln,Zhiwu Shengli Xuebao(Plant Physiology Journal),44(5):951-952(张昊鹏,宋晓涛,张耀,于明冉,田露,李昊阳,左志宇,安晓云,杨雪,孙涛,2008,白银寿的组织培养与快速繁殖,植物生理学报,44(5):951-952)
Zuo Z.Y.,Li J.X.,An X.Y.,Yin X.S.,and Yang X.,2007,Tissue culture and rapid propagation of Haworthia bayeri J.D.Venter&S.A.Hammer,Zhiwu Shengli Xuebao(Plant Physiology Journal),43(2):311-312(左志宇,李建希,安晓云,尹晓爽,2007,杨雪克里克特寿的组织培养与快速繁殖,植物生理学报,43(2):311-312)
Liu F.,Tang Y.H.,Yuan Y.M.,Guo Q.Q.,Shen F.,and Chen J.R.,2016,Tissue culture of the succulent plant Sedum clavatum,Zhiwu Xuebao(Chinese Bulletin of Botany),51(2):251-256(刘芳,唐映红,袁有美,郭清泉,沈帆,陈建荣,2016,多肉植物劳尔的组织培养,植物学报,51(2):251-256)
She Q.H.,Jiang Y.Y.,Yang C.,Gou L.Q.,Zhu B.Y.,and Zhang L.,2018,Research progress on tissue culture and cultivation of plants from Hemsleya Cogn.,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),37(3):1370-1375(佘启惠,姜媛媛,杨程,苟丽琼,朱柏雨,张利,2018,雪胆属植物的组织培养与栽培研究进展,基因组学与应用生物学,37(3):1370-1375)
Tuo Y.,and Bai Y.E.,2018,Callus induction and plant regeneration by shoot tip of Ribes odoratum Wendl,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),16(7):2255-2259(托亚,白玉娥,2018,香茶藨子茎尖愈伤组织诱导及植株再生,分子植物育种,16(7):2255-2259)
Wang Q.,Zuo Z.Y.,Song X.T.,Zhang Y.,Zhang H.P.,Yin X.S.,An X.Y.,Yang X.,and Sun T.,2008,Tissue culture and rapid propagation of Haworthia emelyae var.major(G.G.Smith)M.B.Bayer,Zhiwu Shengli Xuebao(Plant Physiology Journal),44(1):123-124(王泉,左志宇,宋晓涛,张耀,张昊鹏,尹晓爽,安晓云,杨雪,孙涛,2008,百合科多肉植物美吉寿的组织培养与快速繁殖,植物生理学报,44(1):123-124)
Wang T.,2018,Study on rapid propagation technology system with tissue culture of Haworthia cooperi var.truncate,Thesis for M.S.,Gansu Agricultural University,Supervisor:Ma Y.J.,pp.31-56(王婷,2018,姬玉露组织培养快繁技术体系研究,硕士学位论文,甘肃农业大学,导师:马彦军,pp.31-56)
Wang Y.,Xu G.,Wang Y.T.,Mou H.J.,LüY.P.,Chen Z.,and Chen J.P.,2013,Study on the tissue culture and germplasm innovation of Gasteria armstrongi verieg.,Zhejiang Nongye Xuebao(Acta Agriculturae Zhejiangensis),25(3):488-491(王燕,徐刚,汪一婷,牟豪杰,吕永平,陈志,陈剑平,2013,卧牛锦的组织培养及其种质创新初探,浙江农业学报,25(3):488-491)
Wang Z.S.,Wang G.D.,and Wang Y.,2014,Study on in vitro culture and rapid propagation in Haworthia emelyae var.emelyae V.poelln'Miracle',Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),33(6):1329-1335(王紫珊,王广东,王雁,2014,多肉植物白银寿‘奇迹’的离体培养与快速繁殖,基因组学与应用生物学,33(6):1329-1335)
Wu Z.J.,Huang X.J.,Wang B.,Peng T.,and Ma W.Y.,2015,Tissue culture and rapid propagation of Ophthalmophyllum friedrichiae,Zhiwu Shengli Xuebao(Plant Physiology Journal),51(11):2013-2016(吴正景,黄雪娇,王柏,彭涛,马文瑶,2015,风铃玉的组织培养与快速繁殖,植物生理学报,51(11):2013-2016)
Zhang H.P.,Song X.T.,Zhang Y.,Yu M.R.,Tian L.,Li H.Y.,Zuo Z.Y.,An X.Y.,Yang X.,and Sun T.,2008,Tissue culture and rapid propagation of Haworthia emelyae var.emelyae V.poelln,Zhiwu Shengli Xuebao(Plant Physiology Journal),44(5):951-952(张昊鹏,宋晓涛,张耀,于明冉,田露,李昊阳,左志宇,安晓云,杨雪,孙涛,2008,白银寿的组织培养与快速繁殖,植物生理学报,44(5):951-952)
Zuo Z.Y.,Li J.X.,An X.Y.,Yin X.S.,and Yang X.,2007,Tissue culture and rapid propagation of Haworthia bayeri J.D.Venter&S.A.Hammer,Zhiwu Shengli Xuebao(Plant Physiology Journal),43(2):311-312(左志宇,李建希,安晓云,尹晓爽,2007,杨雪克里克特寿的组织培养与快速繁殖,植物生理学报,43(2):311-312)