丝状真菌液体深层发酵菌丝体形态控制研究进展
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摘要
丝状真菌广泛用于工业发酵生产中,其菌丝体形态与发酵液粘度、目的产物产量有着密切关系,是发酵过程控制的关键因素之一。文章综述了丝状真菌液体深层发酵中菌丝体的形态特征及其分析与表征方法、菌丝球形成机理及菌丝体形态控制对发酵影响的研究进展,指出量化描述丝状真菌的生长是精确控制发酵过程的基础。
Filamentous fungi are widely used in industrial fermentation.Its mycelial morphology is closely combined with objective product yield and fermented liquid viscosity,and it is one of the key factors in the fermentation process control.In this paper,the research progress was reviewed on the mycelia of filamentous fungi in the submerged fermentation,including its morphological characteristics,the analysis and characterization methods,the mechanism of mycelial pellet formation,and the influence of mycelial morphology control in fermentation.Therefore,the author indicated that quantitative description the growth of filamentous fungi was the foundation of precision control on fermentation process.
Filamentous fungi are widely used in industrial fermentation.Its mycelial morphology is closely combined with objective product yield and fermented liquid viscosity,and it is one of the key factors in the fermentation process control.In this paper,the research progress was reviewed on the mycelia of filamentous fungi in the submerged fermentation,including its morphological characteristics,the analysis and characterization methods,the mechanism of mycelial pellet formation,and the influence of mycelial morphology control in fermentation.Therefore,the author indicated that quantitative description the growth of filamentous fungi was the foundation of precision control on fermentation process.
引文
[1]MEYER V,WU Bo,RAM ARTHUR F J.Aspergillus as a multipurpose cell factory:current status and perpectives[J].Biotechnol Lett,2011,33(3):469-476.
[2]MYER V.Genetic engineering of filamentous fungi-progress,obstacles and future trends[J].Biotechnol Adv,2008,26:177-185.
[3]WUCHERPFENNIG T,KIEP K A,DRIOUCH H,et al.Chapter 4-morphology and rheology in filamentous cultivations[J].Advances in Applied Microbiology,2010,72:89-136.
[4]OLSVIK E,KRISTIANSEN B.Rheology of filamentous fermentations[J].Biotechnology Advances,1994,12(1):1-39.
[5]CALVO A M,WILSON R A,BOK J W,et al.Relationship between secondary metabolism and fungal development[J].American Society for Microbilogy,2002,66(3):447-459.
[6]PAPAGIANNI M,JOSHI N,MOO-YOUNG M.Comparative studies on extracellular protease secretion and glucoamylase production by free and immobilized Aspergillus niger culture[J].Journal of Industrial Microbiology&Biotechnolgy,2002,29(5):259-263.
[7]RINAS U,EL-ENSHASY H,EMMLER M,et al.Modelbasedperdiction of substrate conversion and protein synthesis andexcretion in recombinant iopellets[J].Chem.Eng.Sci.,2005,60:2 729-2 739.
[8]WANG L P,RIDGWAY D,GU T Y,et al.Bioprecessing strategies to improve heterologous protein production in filamentous fungal fermentations[J].Biotechnol Adv,2005,23(2):115-129.
[9]METWLLY M.Glucoamylase production in continuous culture of Aspergillus niger with special emphasis on growth parameters[J].Microbilogy&Biotechnolgy,1998,14(1):113-118.
[10]RIMS U,EL-ENSHASY H,EMMLER M,et al.Model-based perdiction of substrate conversion and protein synthesis and excretion in recombinant iopellets[J].Chem.Eng.Sci.,2005,60(10):2 729-2 739.
[11]BARRY D J,CHAN C,WILLIAMS G A.Morphological quantification of filamentous fungal development using membrane immobilization and automatic image analysis.Journal of Industrial Microbiology&Biotechnology,2009,36:787.
[12]DU J X,CAO N J,GONG C S,et al.Production of L-Lactic acid by phizopus oryzae in a bubble column ferm entor[J].Appl Biochem Biotechnol,1998,70(72):323-329.
[13]VILLENA G K,GUTIRREZ-CORREA M.Morphological patterns of Aspetgillus niger biofilms and pellets related to lignocellulolytic enzyme productivities[J].Applied Microbiology,2007,45(3):231-237.
[14]LU Fei,PING Kang-kang,WEN Ling,et al.Enhancing gluconic acid production by controlling the morphology of Aspergillus niger in submerged fermentation[J].Process Biochemistry,2015,50(9):1 342-1 348.
[15]LIU Yan,LIAO Wei,CHEN Shu-lin.Study of pellet formation of filamentous fungi Rhizopus oryzae using a multiple logistic regression model[J].Biotechnology and Bioengineering,2008,99(1):117-128.
[16]BRAAKSMA Machtelt,SMILDE Age K,VAN-DER-WERF M J,et al.The effect of environmental conditions on extracellular protease activity in controlled fermentations of Aspergillusniger[J].Microbilogy,2009,155(10):3 430-3 439.
[17]DOMINGUES F C,QUEIROZ J A,CABRAL J M S,et al.The influence of culture conditions on mucelial structure and cellulose production by Trichoderma reesi Rut C-30[J].Enzyme and Microbial Technology,2000,26(5/6):394-401.
[18]ADAMS H L,THOMAS C R.The use of image analysis for morphological measurements on filamentous microorganisms[J].Biotechnology and Bioengineering,1988,32(5):707-712.
[19]RILEY G L,TUCKER K G,PAUL G C,et al.Effect of biomass concentration and mycelial morphology on fermentation broth rheology[J].Biotechnol Bioeng,2000,68(2):160-172.
[20]BRAUN S,VECHT-LIFSHIT Z S E.Mycelial morphology and metabolite production[J].TIBTECH,1991,9(1):63-68.
[21]WARGENAU A,FLEINER André,BOLTEN C J,et al.On the origin of the electrostatic surface potential of Aspergillus niger spores in acidic environments[J].Research in Microbiology,2011,162(10):1 011-1 017.
[22]METZ B,KOSSEN N W F.The growth of molds in the form of pellets:A literature review[J].Biotechnol and Bioeng,1997,110(6):781-799.
[23]LIN P J,GRIMM L H,WULKOW M,et al.Population balance modeling of the conidial aggregation of Aspergillus niger[J].Biotechnology and Bioengineering,2008,99(2):341-350.
[24]LI Z J,SHUKLA V,FORDYCE A P,et al.Fungal morphology and fragmentation behavior in a fed-batch Aspergillus oryzae fermentation at the production scale[J].Biotechnol Bioeng,2000,70(3):300-312.
[25]BARRY D J WILLIAMS G A.Microscopic characterization of filamentous microbes:towards fully automated morphological quantification through image analysis[J].J.Microsc.,2011,244(1):1-20.
[26]唐文俊,夏建业,储炬,等.黑曲霉发酵过程中菌体形态的分析方法建立及应用[J].生物工程学报,2015(2):291-299.
[27]REICHL U,KING R,GILLES E D.Characterization of pellet morphology during submerged growth of Streptomyces tendae by image analysis[J].Biotechnol.and Bioeng.,1992,39(2):164-170.
[28]TRESKATIS S K,ORGELDINGER V,WOLF H,et al.Morphological characterization of filamentous microorganisms in submerged cultures by on-line digital image analysis and pattern recognition[J].Biotechnol.and Bioeng.,1997,53(2):191-201.
[29]YU L,CHAO Y,WENSEL P,et al.Hydrodynamic and kinetic study of cellulase production by Trichoderma reesei with pellet morphology[J].Biotechnol Bioeng,2012,109(7):1 755-1 768.
[30]FENG Kuo-ching,ROU Tzyy-min,LIU Bing-lan,et al.Effect of fungal pellet size on the high yield production of destruxin B by Metarhizium anisopliae[J].Enzyme and Microbial Technology,2004,34(1):22-25.
[31]ANURADHA K,NAGAPADMA P,VENKATESHWAR S,et al.Effect of physical factors on pellet morphology of Aspergillus awamori MTCC 9166and polygalacturonase production[J].Biocatalysis and Agricultural Biotechnology,2014,3(4):271-274.
[32]LIN P J,SCHOLZ Alexandra,KRULL Rainer.Effect of volumetric power input by aeration and agitation on pellet morphology and product formation of Aspergillus niger[J].Biochemical Engineering Journal,2010,49(2):213-220.
[33]RODRGUEZ PORCEL E M,CASAS LPEZ J L,SNCHEZ PERE J A,et al.Effects of pellet morphology on broth rheology in fermentations of Aspergillus terreus[J].Biochemical Engineering Journal,2005,26(2/3):139-144.
[34]CASAS LPEZ J L,SNCHEZ PERE J A,FERNNDEZ SEVILLA J M,et al.Pellet morphology,culture rheology and lovastatin production in cultures of Aspergillus terreus[J].Journal of Biotechnology,2005,116(1):139-144.
[35]赵龙,蒋雪薇,盛灿梅,等.玉米芯载体固定化米根霉发酵L-乳酸工艺的研究[J].食品与机械,2012,28(3):226-229,239.
[36]FENG Kuo-ching,ROU Tzyy-min,LIU Bing-lan,et al.Effect of fungal pellet size on the high yield production of destruxin B by Metarhizium anisopliae[J].Enzyme and Microbial Technology,2004,34(1):22-25.
[37]VILLENA G K,GUTIRREZ-CORREA M.Morphological patterns of Aspetgillus niger biofilms and pellets related to lignocellulolytic enzyme productivities[J].Applied Microbiology,2007,45(3):231-237.
[38]赵龙,蒋雪薇,边佳为,等.玉米芯载体吸附法固定化米根霉发酵L-乳酸的工艺条件[J].食品科学,2013(7):201-205.
[39]ESPINOSA-ORTIZ E J,RENE E R,PAKSHIRAJAN K,et al.Fungal pelleted reactors in wastewater treatment:Applications and perspectives[J].Chemical Engineering Journal,2016,283:553-571.
[40]YU Mei-ching,WANG R C,WANG Chung-yih,et al.Enhanced production of L(+)-lactic acid by floc-form culture of Rhizopus oryzae[J].Journal of the Chinese Institute of Chemieal Engineers,2007,38(3/4):223-228.
[41]蒋雪薇,罗晓明,盛灿梅,等.米根霉L-乳酸发酵菌丝球形成条件的研究[J].食品科学,2010(15):216-220.
[42]YANG Jun,JIAO Rui-hua,YAOA Ling-yun,et al.Control of fungal morphology for improved production of a novel antimicrobial alkaloid by marine-derived fungus Curvularia sp.IFBZ10under submerged fermentation[J].Process Biochemistry,2016,51:185-194.
[2]MYER V.Genetic engineering of filamentous fungi-progress,obstacles and future trends[J].Biotechnol Adv,2008,26:177-185.
[3]WUCHERPFENNIG T,KIEP K A,DRIOUCH H,et al.Chapter 4-morphology and rheology in filamentous cultivations[J].Advances in Applied Microbiology,2010,72:89-136.
[4]OLSVIK E,KRISTIANSEN B.Rheology of filamentous fermentations[J].Biotechnology Advances,1994,12(1):1-39.
[5]CALVO A M,WILSON R A,BOK J W,et al.Relationship between secondary metabolism and fungal development[J].American Society for Microbilogy,2002,66(3):447-459.
[6]PAPAGIANNI M,JOSHI N,MOO-YOUNG M.Comparative studies on extracellular protease secretion and glucoamylase production by free and immobilized Aspergillus niger culture[J].Journal of Industrial Microbiology&Biotechnolgy,2002,29(5):259-263.
[7]RINAS U,EL-ENSHASY H,EMMLER M,et al.Modelbasedperdiction of substrate conversion and protein synthesis andexcretion in recombinant iopellets[J].Chem.Eng.Sci.,2005,60:2 729-2 739.
[8]WANG L P,RIDGWAY D,GU T Y,et al.Bioprecessing strategies to improve heterologous protein production in filamentous fungal fermentations[J].Biotechnol Adv,2005,23(2):115-129.
[9]METWLLY M.Glucoamylase production in continuous culture of Aspergillus niger with special emphasis on growth parameters[J].Microbilogy&Biotechnolgy,1998,14(1):113-118.
[10]RIMS U,EL-ENSHASY H,EMMLER M,et al.Model-based perdiction of substrate conversion and protein synthesis and excretion in recombinant iopellets[J].Chem.Eng.Sci.,2005,60(10):2 729-2 739.
[11]BARRY D J,CHAN C,WILLIAMS G A.Morphological quantification of filamentous fungal development using membrane immobilization and automatic image analysis.Journal of Industrial Microbiology&Biotechnology,2009,36:787.
[12]DU J X,CAO N J,GONG C S,et al.Production of L-Lactic acid by phizopus oryzae in a bubble column ferm entor[J].Appl Biochem Biotechnol,1998,70(72):323-329.
[13]VILLENA G K,GUTIRREZ-CORREA M.Morphological patterns of Aspetgillus niger biofilms and pellets related to lignocellulolytic enzyme productivities[J].Applied Microbiology,2007,45(3):231-237.
[14]LU Fei,PING Kang-kang,WEN Ling,et al.Enhancing gluconic acid production by controlling the morphology of Aspergillus niger in submerged fermentation[J].Process Biochemistry,2015,50(9):1 342-1 348.
[15]LIU Yan,LIAO Wei,CHEN Shu-lin.Study of pellet formation of filamentous fungi Rhizopus oryzae using a multiple logistic regression model[J].Biotechnology and Bioengineering,2008,99(1):117-128.
[16]BRAAKSMA Machtelt,SMILDE Age K,VAN-DER-WERF M J,et al.The effect of environmental conditions on extracellular protease activity in controlled fermentations of Aspergillusniger[J].Microbilogy,2009,155(10):3 430-3 439.
[17]DOMINGUES F C,QUEIROZ J A,CABRAL J M S,et al.The influence of culture conditions on mucelial structure and cellulose production by Trichoderma reesi Rut C-30[J].Enzyme and Microbial Technology,2000,26(5/6):394-401.
[18]ADAMS H L,THOMAS C R.The use of image analysis for morphological measurements on filamentous microorganisms[J].Biotechnology and Bioengineering,1988,32(5):707-712.
[19]RILEY G L,TUCKER K G,PAUL G C,et al.Effect of biomass concentration and mycelial morphology on fermentation broth rheology[J].Biotechnol Bioeng,2000,68(2):160-172.
[20]BRAUN S,VECHT-LIFSHIT Z S E.Mycelial morphology and metabolite production[J].TIBTECH,1991,9(1):63-68.
[21]WARGENAU A,FLEINER André,BOLTEN C J,et al.On the origin of the electrostatic surface potential of Aspergillus niger spores in acidic environments[J].Research in Microbiology,2011,162(10):1 011-1 017.
[22]METZ B,KOSSEN N W F.The growth of molds in the form of pellets:A literature review[J].Biotechnol and Bioeng,1997,110(6):781-799.
[23]LIN P J,GRIMM L H,WULKOW M,et al.Population balance modeling of the conidial aggregation of Aspergillus niger[J].Biotechnology and Bioengineering,2008,99(2):341-350.
[24]LI Z J,SHUKLA V,FORDYCE A P,et al.Fungal morphology and fragmentation behavior in a fed-batch Aspergillus oryzae fermentation at the production scale[J].Biotechnol Bioeng,2000,70(3):300-312.
[25]BARRY D J WILLIAMS G A.Microscopic characterization of filamentous microbes:towards fully automated morphological quantification through image analysis[J].J.Microsc.,2011,244(1):1-20.
[26]唐文俊,夏建业,储炬,等.黑曲霉发酵过程中菌体形态的分析方法建立及应用[J].生物工程学报,2015(2):291-299.
[27]REICHL U,KING R,GILLES E D.Characterization of pellet morphology during submerged growth of Streptomyces tendae by image analysis[J].Biotechnol.and Bioeng.,1992,39(2):164-170.
[28]TRESKATIS S K,ORGELDINGER V,WOLF H,et al.Morphological characterization of filamentous microorganisms in submerged cultures by on-line digital image analysis and pattern recognition[J].Biotechnol.and Bioeng.,1997,53(2):191-201.
[29]YU L,CHAO Y,WENSEL P,et al.Hydrodynamic and kinetic study of cellulase production by Trichoderma reesei with pellet morphology[J].Biotechnol Bioeng,2012,109(7):1 755-1 768.
[30]FENG Kuo-ching,ROU Tzyy-min,LIU Bing-lan,et al.Effect of fungal pellet size on the high yield production of destruxin B by Metarhizium anisopliae[J].Enzyme and Microbial Technology,2004,34(1):22-25.
[31]ANURADHA K,NAGAPADMA P,VENKATESHWAR S,et al.Effect of physical factors on pellet morphology of Aspergillus awamori MTCC 9166and polygalacturonase production[J].Biocatalysis and Agricultural Biotechnology,2014,3(4):271-274.
[32]LIN P J,SCHOLZ Alexandra,KRULL Rainer.Effect of volumetric power input by aeration and agitation on pellet morphology and product formation of Aspergillus niger[J].Biochemical Engineering Journal,2010,49(2):213-220.
[33]RODRGUEZ PORCEL E M,CASAS LPEZ J L,SNCHEZ PERE J A,et al.Effects of pellet morphology on broth rheology in fermentations of Aspergillus terreus[J].Biochemical Engineering Journal,2005,26(2/3):139-144.
[34]CASAS LPEZ J L,SNCHEZ PERE J A,FERNNDEZ SEVILLA J M,et al.Pellet morphology,culture rheology and lovastatin production in cultures of Aspergillus terreus[J].Journal of Biotechnology,2005,116(1):139-144.
[35]赵龙,蒋雪薇,盛灿梅,等.玉米芯载体固定化米根霉发酵L-乳酸工艺的研究[J].食品与机械,2012,28(3):226-229,239.
[36]FENG Kuo-ching,ROU Tzyy-min,LIU Bing-lan,et al.Effect of fungal pellet size on the high yield production of destruxin B by Metarhizium anisopliae[J].Enzyme and Microbial Technology,2004,34(1):22-25.
[37]VILLENA G K,GUTIRREZ-CORREA M.Morphological patterns of Aspetgillus niger biofilms and pellets related to lignocellulolytic enzyme productivities[J].Applied Microbiology,2007,45(3):231-237.
[38]赵龙,蒋雪薇,边佳为,等.玉米芯载体吸附法固定化米根霉发酵L-乳酸的工艺条件[J].食品科学,2013(7):201-205.
[39]ESPINOSA-ORTIZ E J,RENE E R,PAKSHIRAJAN K,et al.Fungal pelleted reactors in wastewater treatment:Applications and perspectives[J].Chemical Engineering Journal,2016,283:553-571.
[40]YU Mei-ching,WANG R C,WANG Chung-yih,et al.Enhanced production of L(+)-lactic acid by floc-form culture of Rhizopus oryzae[J].Journal of the Chinese Institute of Chemieal Engineers,2007,38(3/4):223-228.
[41]蒋雪薇,罗晓明,盛灿梅,等.米根霉L-乳酸发酵菌丝球形成条件的研究[J].食品科学,2010(15):216-220.
[42]YANG Jun,JIAO Rui-hua,YAOA Ling-yun,et al.Control of fungal morphology for improved production of a novel antimicrobial alkaloid by marine-derived fungus Curvularia sp.IFBZ10under submerged fermentation[J].Process Biochemistry,2016,51:185-194.