基于中药整合药理学平台分析黄连汤治疗胃炎的作用机制
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摘要
目的:探讨黄连汤治疗胃炎的功效物质基础和可能的作用机制,为该复方的后续应用与研发提供参考。方法:利用中药整合药理学平台预测黄连汤治疗胃炎的主要活性成分和作用靶点,构建黄连汤成分靶标-疾病靶标网络,筛选关键节点进行通路富集分析,探索黄连汤多成分-多靶点-多通路治疗胃炎的可能作用机制。结果:黄连汤预测的175个活性成分与胃炎相关的538个关键靶标相互作用,通过趋化因子、雌激素和T细胞受体等信号通路参与幽门螺旋杆菌感染侵润、胃黏膜损伤、胃黏膜萎缩等胃炎发生与发展过程中病理环节的调控。结论:该研究初步揭示了黄连汤治疗胃炎的潜在活性成分及其可能的作用机制,为该方进一步的药效物质基础和作用机制实验研究提供了理论依据。
Objective:To investigate the effective substance basis and possible mechanism of Huangliantang in treatment of gastritis.Method:Integrated pharmacology platform of traditional Chinese medicine was employed to predict the main active ingredients and functional targets of Huangliantang in treatment of gastritis,network of composition target-disease target of Huangliantang was constructed,key nodes were screened for enrichment analysis of pathways,and the possible mechanism of Huangliantang in treatment of gastritis with multiple ingredients-multiple targets-multiple pathways was explored.Result:A total of 175 predicted active ingredients of Huangliantang interacted with 538 key targets about gastritis,the regulation and treatment of gastritis during its different pathological stages,such as Helicobacter pylori infection,gastric mucosal damage and gastric mucosal atrophy,were involved through chemokine,T cell receptor,estrogen and other signaling pathways.Conclusion:This research may reveal the potential active ingredients of Huangliantang in treatment of gastritis and its possible mechanism,and it also provides a theoretical basis for further experimental research of pharmacodynamic substance basis and mechanism of action.
Objective:To investigate the effective substance basis and possible mechanism of Huangliantang in treatment of gastritis.Method:Integrated pharmacology platform of traditional Chinese medicine was employed to predict the main active ingredients and functional targets of Huangliantang in treatment of gastritis,network of composition target-disease target of Huangliantang was constructed,key nodes were screened for enrichment analysis of pathways,and the possible mechanism of Huangliantang in treatment of gastritis with multiple ingredients-multiple targets-multiple pathways was explored.Result:A total of 175 predicted active ingredients of Huangliantang interacted with 538 key targets about gastritis,the regulation and treatment of gastritis during its different pathological stages,such as Helicobacter pylori infection,gastric mucosal damage and gastric mucosal atrophy,were involved through chemokine,T cell receptor,estrogen and other signaling pathways.Conclusion:This research may reveal the potential active ingredients of Huangliantang in treatment of gastritis and its possible mechanism,and it also provides a theoretical basis for further experimental research of pharmacodynamic substance basis and mechanism of action.
引文
[1]胡心康.黄连汤加减辨证治疗慢性胃炎[J].中医临床研究,2017,9(4):110-111.
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[6]王如茂.黄连汤合左金丸加味治疗胆汁反流性胃炎30例[J].中国中医药信息杂志,2008,15(6):75-76.
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[13]何甜,唐慧,郭强,等.趋化因子10在慢性非萎缩性胃炎、胃癌癌前病变及胃癌中的表达及意义[J].重庆医学,2014,43(4):388-390.
[14]田黎黎,师水生.慢性胃炎中黏膜相关上皮趋化因子28与白细胞介素-10的表达及与幽门螺杆菌的关系[J].中国药物与临床,2010,10(2):200-202.
[15]何甜,唐慧,杨慧,等.趋化因子受体4和6在慢性浅表性胃炎、癌前病变及胃癌中的表达及意义[J].中华临床医师杂志:电子版,2011,5(18):5299-5304.
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[18]Harakal J,Rival C,QIAO H,et al.Regulatory T cells control Th2-dominant murine autoimmune gastritis[J].JImmunol,2016,197(1):27-41.
[19]梁娜,孙少平,罗跃娥,等.阿魏酸的研究进展[J].黑龙江中医药,2009,38(3):39-40.
[20]蒋淑英.阿魏酸钠治疗慢性萎缩性胃炎的临床疗效[J].中国现代药物应用,2010,4(13):137-138.
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[22]蔡泳锋,连大卫,苏锐,等.氧化应激与幽门螺杆菌感染相关性胃炎[J].现代生物医学进展,2016,16(27):5397-5400.
[23]赵唯含,史瑞,杨美娟,等.黄芪甲苷、人参皂苷Rg1对慢性萎缩性胃炎大鼠Hedgehog信号通路的调控影响[J].环球中医药,2017,10(12):1428-1433.
[24]杨庆,李玉洁,陈颖,等.大枣提取物对缺铁性贫血大鼠的保护作用[J].中国实验方剂学杂志,2017,23(3):102-109.
[25]肖志彬,刘小雷,成日青,等.β-谷甾醇对阿司匹林致胃黏膜损伤副作用及其药理作用的影响[J].中国实验方剂学杂志,2016,22(1):148-152.
[26]张洋.甘草酸对BALB/c小鼠胃粘膜Hp感染的抑制作用[D].长春:吉林大学,2009.
[27]刘守义,尤春来,王义明.半夏抗溃疡作用机理的实验研究[J].辽宁中医杂志,1992,19(10):42-45.
[28]蒋苏贞,廖康.干姜醇提取物对实验性胃溃疡的影响[J].中国民族民间医药,2010,19(8):79-80.
[2]刘丰晓.黄连汤治疗慢性胃炎40例[J].中国中医药现代远程教育,2010,8(6):37.
[3]赵庆新,梁宝慧,卢燕许.黄连汤治疗幽门螺旋杆菌相关性慢性浅表性胃炎48例[J].河南中医药学刊,2000,15(4):28-29.
[4]杨光成.黄连汤治疗慢性萎缩性胃炎54例[J].福建中医药,2007,38(3):35-36.
[5]周自祥.黄连汤加味治疗慢性萎缩性胃炎53例[J].广西中医学院学报,2009,12(3):14-15.
[6]王如茂.黄连汤合左金丸加味治疗胆汁反流性胃炎30例[J].中国中医药信息杂志,2008,15(6):75-76.
[7]许海玉,刘振明,付岩,等.中药整合药理学计算平台的开发与应用[J].中国中药杂志,2017,42(18):3633-3638.
[8]de Luis D A,de la Calle H,Roy G,et al.Helicobacter pylori infection and insulin-dependent diabetes mellitus[J].Diabetes Res Clin Pract,1998,39(2):143-146.
[9]范衡,吴永梅.糖脂代谢紊乱与慢性萎缩性胃炎的相关性研究[J].新疆医学,2015,45(11):1691-1693.
[10]ZHAO W,ZHU F W,FU A,et al.Protective effects of DIDS against ethanol-induced gastric mucosal injury in rats[J].Acta Biochim Biophys Sin(Shanghai),2009,41(4):301-308.
[11]PAN J S,HE S Z,XU H Z,et al.Oxidative stress disturbs energy metabolism of mitochondria in ethanolinduced gastric mucosa injury[J].World JGastroenterol,2008,14(38):5857-5867.
[12]段德民,熊凌霜,吴军.膜外核苷酸焦磷酸酯酶/磷酸二酯酶家族的研究进展[J].生物技术通讯,2007,18(2):277-280.
[13]何甜,唐慧,郭强,等.趋化因子10在慢性非萎缩性胃炎、胃癌癌前病变及胃癌中的表达及意义[J].重庆医学,2014,43(4):388-390.
[14]田黎黎,师水生.慢性胃炎中黏膜相关上皮趋化因子28与白细胞介素-10的表达及与幽门螺杆菌的关系[J].中国药物与临床,2010,10(2):200-202.
[15]何甜,唐慧,杨慧,等.趋化因子受体4和6在慢性浅表性胃炎、癌前病变及胃癌中的表达及意义[J].中华临床医师杂志:电子版,2011,5(18):5299-5304.
[16]Sheh A,GE Z,Parry N M,et al.17β-Estradiol and tamoxifen prevent gastric cancer by modulating leukocyte recruitment and oncogenic pathways in Helicobacter pylori-infected INS-GAS male mice[J].Cancer Prev Res(Phila),2011,4(9):1426-1435.
[17]周槐娜,郑春英,黄捷平.不同类型的慢性胃炎和T淋巴细胞亚群的关系[J].中国现代医生,2007,45(23):28-29.
[18]Harakal J,Rival C,QIAO H,et al.Regulatory T cells control Th2-dominant murine autoimmune gastritis[J].JImmunol,2016,197(1):27-41.
[19]梁娜,孙少平,罗跃娥,等.阿魏酸的研究进展[J].黑龙江中医药,2009,38(3):39-40.
[20]蒋淑英.阿魏酸钠治疗慢性萎缩性胃炎的临床疗效[J].中国现代药物应用,2010,4(13):137-138.
[21]刘秋伟,梁菊,陈攀.天然抗氧化剂对ONOO-损伤的抑制效应[J].生命的化学,2010,30(1):92-95.
[22]蔡泳锋,连大卫,苏锐,等.氧化应激与幽门螺杆菌感染相关性胃炎[J].现代生物医学进展,2016,16(27):5397-5400.
[23]赵唯含,史瑞,杨美娟,等.黄芪甲苷、人参皂苷Rg1对慢性萎缩性胃炎大鼠Hedgehog信号通路的调控影响[J].环球中医药,2017,10(12):1428-1433.
[24]杨庆,李玉洁,陈颖,等.大枣提取物对缺铁性贫血大鼠的保护作用[J].中国实验方剂学杂志,2017,23(3):102-109.
[25]肖志彬,刘小雷,成日青,等.β-谷甾醇对阿司匹林致胃黏膜损伤副作用及其药理作用的影响[J].中国实验方剂学杂志,2016,22(1):148-152.
[26]张洋.甘草酸对BALB/c小鼠胃粘膜Hp感染的抑制作用[D].长春:吉林大学,2009.
[27]刘守义,尤春来,王义明.半夏抗溃疡作用机理的实验研究[J].辽宁中医杂志,1992,19(10):42-45.
[28]蒋苏贞,廖康.干姜醇提取物对实验性胃溃疡的影响[J].中国民族民间医药,2010,19(8):79-80.
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