红龙草色素的提取工艺优化及其稳定性研究
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摘要
[目的]优化红龙草色素提取工艺,并研究其稳定性。[方法]以红龙草全草为材料,通过单因素试验和正交试验优化红龙草色素提取工艺,并对提取的红龙草色素稳定性进行研究。[结果]红龙草色素的最佳提取条件:超声时间30 min,料液比1∶55(g∶mL),α-淀粉酶∶纤维素酶为3∶1,即在溶液中按比例加入0.2%纤维素酶和0.6%α-淀粉酶。光照、pH、过氧化氢、亚硫酸钠、苯甲酸、山梨酸、金属离子Zn~(2+)、Cu~(2+)、Ca~(2+)、Fe~(3+)对色素稳定性具有一定的影响。[结论]该研究可为红龙草色素的开发利用提供参考。
[Objective] To optimize the extraction process of Altemanthera Ficoidea cv. Ruliginosa pigment and study its stability. [Method]By single-factor and L_9(3~4) orthogonal experiment,extraction conditions of pigment from Altemanthera Ficoidea cv. Ruliginosa were optimized,and the effects on the stability of pigment were investigated. [Result] The results showed that the optimum technological conditions of the extraction of pigment from Altemanthera Ficoidea cv. Ruliginosa were distilled water for extraction agents,30 min for extraction time,1∶ 55(g∶ mL) for the ratio of the solid and liquid,1∶ 3 for the ratio of cellulose to alpha amylase,namely adding 0. 2% cellulose and 0. 6% alpha amylase into solution. Light,pH,H_2O_2,sulfurous acid,benzoic acid,sorbic acid,metal ions including Zn~(2+),Cu~(2+),Ca~(2+)and Fe~(3+)had effects on the stability of pigment. [Conclusion] The study can provide reference for development and utilization of Altemanthera Ficoidea cv.Ruliginosa pigment.
[Objective] To optimize the extraction process of Altemanthera Ficoidea cv. Ruliginosa pigment and study its stability. [Method]By single-factor and L_9(3~4) orthogonal experiment,extraction conditions of pigment from Altemanthera Ficoidea cv. Ruliginosa were optimized,and the effects on the stability of pigment were investigated. [Result] The results showed that the optimum technological conditions of the extraction of pigment from Altemanthera Ficoidea cv. Ruliginosa were distilled water for extraction agents,30 min for extraction time,1∶ 55(g∶ mL) for the ratio of the solid and liquid,1∶ 3 for the ratio of cellulose to alpha amylase,namely adding 0. 2% cellulose and 0. 6% alpha amylase into solution. Light,pH,H_2O_2,sulfurous acid,benzoic acid,sorbic acid,metal ions including Zn~(2+),Cu~(2+),Ca~(2+)and Fe~(3+)had effects on the stability of pigment. [Conclusion] The study can provide reference for development and utilization of Altemanthera Ficoidea cv.Ruliginosa pigment.
引文
[1]刘德良.红苋草色素提取工艺及稳定性研究[J].湖北农业科学,2012,51(1):143-146.
[2]郑华,张弘,张忠和.天然动植物色素的特性及其提取技术概况[J].林业科学研究,2003,16(5):628-635.
[3]赵昶灵,陈俊愉,刘雪兰,等.理化因素对梅花‘南京红须’花色色素颜色呈现的效应[J].南京林业大学学报(自然科学版),2004,28(2):27-32.
[4]顾文秀,谢为明,夏文水,等.超声波提取乌饭树叶色素及其稳定性的研究[J].林产化学与工业,2005,25(4):74-78.
[5]李桂新,杨志孝,孙兆武.牵牛花色素色的超声提取及稳定性研究[J].山东农业大学学报(自然科学版),2004,35(2):205-207.
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[8]蔡金星,刘秀凤,李兆蒙,等.以微波-超声波法提取草莓色素及对其理化性质的研究[J].食品与发酵工业,2003,29(5):69-72.
[9]崔蕊静,李凤英,李春华.黑豆皮红色素提取及影响其稳定性因素分析[J].中国粮油学报,2004,19(1):48-51.
[10]盛锋,付蕾,李长城,等.连翘花黄色素的提取及其对光稳定性的研究[J].食品与发酵工业,2002,28(2):66-70.
[11]于加平.藿香色素的提取及稳定性研究[J].北方园艺,2011(24):35-37.
[12]吕晓玲,王玉平,周平,等.甜菜红色素主要成分抗氧化能力[J].食品研究与开发,2009,30(6):39-43.
[13]王玲,邓敏姬.紫甘薯天然红色素的提取及其稳定性研究[J].食品科技,2011,36(4):179-183.
[14]彭子模,惠寿年,李进,等.玫瑰花红色素及其稳定性研究[J].西北植物学报,1999,19(2):349-353.
[15]詹福建,巫光宏,黄卓烈,等.红龙草红色素稳定性的研究[J]`.植物资源与环境学报,2006,15(2):60-62,67.
[2]郑华,张弘,张忠和.天然动植物色素的特性及其提取技术概况[J].林业科学研究,2003,16(5):628-635.
[3]赵昶灵,陈俊愉,刘雪兰,等.理化因素对梅花‘南京红须’花色色素颜色呈现的效应[J].南京林业大学学报(自然科学版),2004,28(2):27-32.
[4]顾文秀,谢为明,夏文水,等.超声波提取乌饭树叶色素及其稳定性的研究[J].林产化学与工业,2005,25(4):74-78.
[5]李桂新,杨志孝,孙兆武.牵牛花色素色的超声提取及稳定性研究[J].山东农业大学学报(自然科学版),2004,35(2):205-207.
[6]李和生,王鸿飞.桑椹红色素的提取工艺及其稳定性研究[J].食品科技,2002(3):51-52.
[7]徐忠,薄凯.千日红花色苷的酶法提取及纯化研究[J].食品与发酵工业,2006,32(8):139-141.
[8]蔡金星,刘秀凤,李兆蒙,等.以微波-超声波法提取草莓色素及对其理化性质的研究[J].食品与发酵工业,2003,29(5):69-72.
[9]崔蕊静,李凤英,李春华.黑豆皮红色素提取及影响其稳定性因素分析[J].中国粮油学报,2004,19(1):48-51.
[10]盛锋,付蕾,李长城,等.连翘花黄色素的提取及其对光稳定性的研究[J].食品与发酵工业,2002,28(2):66-70.
[11]于加平.藿香色素的提取及稳定性研究[J].北方园艺,2011(24):35-37.
[12]吕晓玲,王玉平,周平,等.甜菜红色素主要成分抗氧化能力[J].食品研究与开发,2009,30(6):39-43.
[13]王玲,邓敏姬.紫甘薯天然红色素的提取及其稳定性研究[J].食品科技,2011,36(4):179-183.
[14]彭子模,惠寿年,李进,等.玫瑰花红色素及其稳定性研究[J].西北植物学报,1999,19(2):349-353.
[15]詹福建,巫光宏,黄卓烈,等.红龙草红色素稳定性的研究[J]`.植物资源与环境学报,2006,15(2):60-62,67.