QuEChERS-超高效液相色谱法测定水样中吡虫啉和虫螨腈
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摘要
[目的]建立一种QuEChERS方法前处理结合超高效液相色谱同时检测水中吡虫啉和虫螨腈含量的分析方法。[方法]乙腈作为提取剂,PSA进行净化,通过超高效液相色谱,以乙腈-水(体积比85∶15)为流动相,使用C18色谱柱,在270 nm波长下对吡虫啉和虫螨腈进行定量分析。[结果]吡虫啉和虫螨腈的线性相关系数分别为1.0000和0.9997;2种药在水中的添加回收率为91.3%～95.1%,相对标准偏差为2.6%～4.7%。[结论]该方法精密度、准确度高,适用于吡虫啉和虫螨腈的定量分析。
[Aims] A ultra performance liquid chromatography(UPLC) method for determination of imidacloprid and chlorfenapyr was established by optimization of QuEChERS pretreatment method. [Methods] The water was extracted with acetonitrile, purified by PSA and determination was carried out on a C_(18) column with mobile phase of acetonitrile-water(85∶15, by vol) at 270 nm. [Results ] The results showed that the linear correlation coefficients of imidacloprid and chlorfenapyr were 1.0000 and 0.9997, the recovery of the two pesticides in water was 91.3-95.1%,and the relative standard deviation was 2.6-4.7%. [Conclusions] The methods has high precision and accuracy, which is suitable for quantitative analysis of imidacloprid and chlorfenapyr.
[Aims] A ultra performance liquid chromatography(UPLC) method for determination of imidacloprid and chlorfenapyr was established by optimization of QuEChERS pretreatment method. [Methods] The water was extracted with acetonitrile, purified by PSA and determination was carried out on a C_(18) column with mobile phase of acetonitrile-water(85∶15, by vol) at 270 nm. [Results ] The results showed that the linear correlation coefficients of imidacloprid and chlorfenapyr were 1.0000 and 0.9997, the recovery of the two pesticides in water was 91.3-95.1%,and the relative standard deviation was 2.6-4.7%. [Conclusions] The methods has high precision and accuracy, which is suitable for quantitative analysis of imidacloprid and chlorfenapyr.
引文
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[8]叶倩,邓义才,路大海,等.气相色谱法测定蔬菜水果中氟虫腈等6种农药残留[J].农药, 2016, 55(1):51-54.
[9]王兵,李斌,张媛媛,等. QuEChERS-高效液相色谱法测定绿茶中虫螨腈残留[J].中国食品学报, 2017, 17(1):241-245.
[10]张晟,瑞潘艳,李重九,等.气相色谱-负化学电离质谱法(GC-NCIMS)对虫螨腈在茄子和土壤中残留的分析[J].分析测试学报, 2008, 27(S1):134-136.
[2]张雪辉,杜雨倩,徐芳,等.高效液相色谱法检测蔬菜中的吡虫啉残留[J].现代食品科技, 2012, 4(28):466-468.
[3]王孝辉,宛晓春,侯如燕.茶叶中吡虫啉农药残留的液相色谱检测方法[J].茶业科学, 2012, 32(3):203-209.
[4]谢文,丁慧瑛,蒋晓英,等.液相色谱串联质谱检测蔬菜和茶叶中吡虫啉的残留量[J].色谱, 2006, 24(6):633-635.
[5]李广领,姜金庆,王自良,等.气相色谱法检测卷心菜中啶虫脒和吡虫啉残留[J].西北农业学报, 2010(9):71-74.
[6]陈迎丽,林绍霞,宋光林,等. HPLC-MS/MS法分析18%吡虫啉·氟酰脲悬浮剂含量[J].农药, 2016, 55(12):898-900.
[7]陈九星,曹永松,王跃龙,等.溴虫腈在甘蓝及土壤中的残留检测及降解动态[J].环境科学学报, 2005(10):1373-1377.
[8]叶倩,邓义才,路大海,等.气相色谱法测定蔬菜水果中氟虫腈等6种农药残留[J].农药, 2016, 55(1):51-54.
[9]王兵,李斌,张媛媛,等. QuEChERS-高效液相色谱法测定绿茶中虫螨腈残留[J].中国食品学报, 2017, 17(1):241-245.
[10]张晟,瑞潘艳,李重九,等.气相色谱-负化学电离质谱法(GC-NCIMS)对虫螨腈在茄子和土壤中残留的分析[J].分析测试学报, 2008, 27(S1):134-136.