不同价态铁处理腈纶废水过程中菌群结构分析
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摘要
利用不同反应器条件(SBBR、Fe(0)-SBBR、Fe(Ⅱ)-SBBR、Fe(Ⅲ)-SBBR)对腈纶废水进行处理,探究不同价态铁对腈纶废水处理过程及此过程中微生物群落结构变化.结果表明,Fe(0)/Fe(Ⅱ)/Fe(Ⅲ)-SBBR对腈纶废水有良好的处理效果,特别是NH_4~+-N,去除率均在90%以上;整个运行周期内Fe(0)-SBBR处理效果最好.利用Illumina Mi Seq高通量测序技术分析处理过程中微生物群落结构,结果表明,Fe(0)/Fe(Ⅱ)/Fe(Ⅲ)-SBBR优势菌在属水平上差异显著,Fe(0)-SBBR主要以Gemmata、Planctomyces、Aridibacter、Fluviicola等属为主;Fe(Ⅱ)-SBBR主要以Thermomonas、Aridibacter、Bacillus、Paracoccus等属为主;Fe(Ⅲ)-SBBR主要以Planctomyces、Bacillus、Nostocoida、Aridibacter等属为主;与对照组SBBR相比,Fe(0)-SBBR对其处于相对劣势的菌有很好的刺激生长作用;Fe(0)和Fe(Ⅲ)对微生物群落的改变大于Fe(Ⅱ).
Different reactors(SBBR, Fe(0)-SBBR, Fe(Ⅱ)-SBBR, Fe(Ⅲ)-SBBR) were employed in this paper to investigate the treating processes of acrylic fiber wastewater by different valence forms of iron as well as the variation of microbial communities during these processes. The results showed that acrylic fiber wastewater was well treated by Fe(0)/Fe(Ⅱ)/Fe(Ⅲ)-SBBR, especially the removal rate of NH_4~+-N was above 90%. And, the Fe(0)-SBBR worked best throughout the entire operating cycle. Illumina Mi Seq high throughput sequencing was also utilized to analyze the structure of microbial communities during the processes. It was found that the dominant bacteria were significantly different at the genus level between Fe(0)/Fe(Ⅱ)/Fe(Ⅲ)-SBBR systems. Gemmata、Planctomyces、Aridibacter、Fluviicola were the dominant genera in Fe(0)-SBBR. Thermomonas、Aridibacter、Bacillus、Paracoccus were the dominant genera in Fe(Ⅱ)-SBBR. Planctomyces、Bacillus、Nostocoida、Aridibacter were the dominant genera in Fe(Ⅲ)-SBBR. Compared with control group SBBR, Fe(0)-SBBR could strongly stimulate the growth of bacteria which were at a relative disadvantage. Moreover, the change of microbial community by Fe(0) and Fe(Ⅲ) were greater than that by Fe(Ⅱ).
Different reactors(SBBR, Fe(0)-SBBR, Fe(Ⅱ)-SBBR, Fe(Ⅲ)-SBBR) were employed in this paper to investigate the treating processes of acrylic fiber wastewater by different valence forms of iron as well as the variation of microbial communities during these processes. The results showed that acrylic fiber wastewater was well treated by Fe(0)/Fe(Ⅱ)/Fe(Ⅲ)-SBBR, especially the removal rate of NH_4~+-N was above 90%. And, the Fe(0)-SBBR worked best throughout the entire operating cycle. Illumina Mi Seq high throughput sequencing was also utilized to analyze the structure of microbial communities during the processes. It was found that the dominant bacteria were significantly different at the genus level between Fe(0)/Fe(Ⅱ)/Fe(Ⅲ)-SBBR systems. Gemmata、Planctomyces、Aridibacter、Fluviicola were the dominant genera in Fe(0)-SBBR. Thermomonas、Aridibacter、Bacillus、Paracoccus were the dominant genera in Fe(Ⅱ)-SBBR. Planctomyces、Bacillus、Nostocoida、Aridibacter were the dominant genera in Fe(Ⅲ)-SBBR. Compared with control group SBBR, Fe(0)-SBBR could strongly stimulate the growth of bacteria which were at a relative disadvantage. Moreover, the change of microbial community by Fe(0) and Fe(Ⅲ) were greater than that by Fe(Ⅱ).
引文
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[2]Wang S,Yu Z C,Ma D.Acrylic fiber wastewater treatment in a nested biofilm airlift suspension reactor[J].International Journal of Applied Environmental Sciences,2013,8(19):2379.
[3]Zhang C Y,Wang J L,Zhou H F,et al.Anodic treatment of acrylic fiber manufacturing wastewater with boron-doped diamond electrode:A statistical approach[J].Chemical Engineering Journal,2010,161(1/2):93-98.
[4]Li J,Luan Z K,Yu L,et al.Pretreatment of acrylic fiber manufacturing wastewater by the Fenton process[J].Desalination,2012,284:62-65.
[5]Kong Q,Ngo H H,Shu L,et al.Enhancement of aerobic granulation by zero-valent iron in sequencing batch airlift reactor[J].Journal of Hazardous Materials,2014,279:511-517.
[6]王亚娥,李杰,翟思媛,等.Fe0对SBBR工艺处理腈纶废水性能影响[J].化工学报,2013,64(8):2996-3002.
[7]Zhen B,Qiao S,Zhou J,et al.Fast start-up of anammox process with appropriate ferrous iron concentration[J].Bioresource Technology,2014,170(5):506-512.
[8]Zhang J,Zhang Y,Quan X,et al.Effects of ferric iron on the anaerobic treatment and microbial biodiversity in a coupled microbial electrolysis cell(MEC):Anaerobic reactor[J].Water Research,2013,47(15):5719-5728.
[9]汪桂芝,戴友芝,龚敏,等.不同价态铁元素对厌氧微生物降解2,4,6-三氯酚的影响[J].微生物学通报,2013,40(12):2196-2202.
[10]Philip A,Li J Z,Portia O B,et al.Dosing effect of zero valent iron(ZVI)on biomethanation and microbial community distribution as revealed by 16S r RNA highthroughput sequencing[J].International Biodeterioration&Biodegradation,2017,123:191-199.
[11]Ru W,Cheng Y,Meng Z,et al.Chemoautotrophic denitrification based on ferrous iron oxidation:Reactor performance and sludge characteristics[J].Chemical Engineering Journal,2017,313:693-701.
[12]白廷洲.腈纶废水生物强化处理研究[D].兰州:兰州交通大学,2016.
[13]方自磊.外加碳源及不同运行工艺对腈纶废水处理研究[D].兰州:兰州交通大学,2017.
[14]李杰,王亚娥.生物海绵铁的制备及其在水处理中的应用:中国,ZL2006 1 0104990.9[P].2009-04-29.
[15]豆宁龙.不同价态铁对生化处理强化作用研究[D].兰州:兰州交通大学,2014.
[16]李杰,张艳梅,王亚娥.零价铁载体填料主料结合方式对生物强化效果的影响研究[J].水处理技术,2015,41(3):41-44.
[17]权海荣,王亚娥,赵炜,等.海绵铁加量对SBSI反应器处理性能的影响[J].环境科学与技术,2017,44(4):148-151.
[18]王秀蘅,任南琪,王爱杰,等.铁锰离子对硝化反应的影响效应研究[J].哈尔滨工业大学学报,2003,35(1):122-125.
[19]Wu D L,Shen Y H,Ding A Q,et al.Effects of nanoscale zero-valent iron particles on biological nitrogen and phosphorus removal and microorganisms in activated sludge[J].Journal of Hazardous Materials,2013,262:649-655.
[20]Hu M,Wang X H,Wen X H,et al.Microbial community structures in different wastewater treatment plants as revealed by 454-pyrosequencing analysis[J].Bioresource Technology,2012,117:72-79.
[21]Ma Q,Qu Y,Shen W,et al.Bacterial community compositions of coking wastewater treatment plants in steel industry revealed by Illumina high-throughput sequencing[J].Bioresource Technology,2015,179:436-443.
[22]Wang X H,Wen X H,Yan H J,et al.Bacterial Community Dynamics in a Functionally Stable Pilot-scale Wastewater Treatment Plant[J].Bioresource Technology,2011,102(3):2352-2357.
[23]Wong M T,Mino T,Seviour R J,et al.In situidentification and characterization of the microbial community structure of full-scale enhanced biological phosphorous removal plants in Japan[J].Water Research,2005,39(13):2901-2914.
[24]Zhang T,Shao M F,Ye L.454Pyrosequencing reveals bacterial diversity of activated sludge from 14sewage treatment plants[J].ISME Journal,2012,6(6):1137-1147.
[25]张楠,初里冰,丁鹏元,等.A/O生物膜法强化处理石化废水及生物膜种群结构研究[J].中国环境科学,2015,35(1):80-86.
[26]Wang Y,Zhu G B,Erwin van der Biezen,et al.Microbial Diversity of planctomycetes and related bacteria in wetlands with different anthropogenic disturbances[J].Wet Land Science,2013,11(2):158-166.
[27]Thomas F,Hehemann J H,Rebuffet E,et al.Environmental and gut Bacteroidetes:the food connection[J].Frontiers in Microbiology,2011,2:1-16.
[28]Liu X T,Yin H,Tang S Y,et al.Effects of single and combined copper/perfluorooctane sulfonate on sequencing batch reactor process and microbial community in activated sludge[J].Bioresource Technology,2017,238:407-415.
[29]范军辉,郝瑞霞,Liu L,等.SCSC-S/Fe复合系统脱氮除磷途径及微生物群落特性[J].中国环境科学,2017,37(4):1358-1365.
[30]Foesel B U,Overmann J,Huber K J,et al.Particulate organic matter(POM)degradation in acidobacteria is species-specific[R].German:German Association for General and Applied Microbiology(VAAM),2017.
[31]Eva M M,Simone G,Bernhard S,et al.Ecophysiology and the energetic benefit of mixotrophic Fe(Ⅱ)oxidation by various strains of nitrate-reducing bacteria[J].FEMS Microbiol Ecol,2009,70:335–343.
[32]Michael W,Alexander L.Bacterial community composition and function in sewage treatment systems[J].Current opinion in biotechnology,2002,13(3):218-227.
[33]Liu J B,Zhang H B,Zhang P Y,et al.Two-stage anoxic/oxic combined membrane bioreactor system for landfill leachate treatment:Pollutant removal performances and microbial community[J].Bioresource Technology,2017,243:738-746.