培养方式对废水脱氮与沼气脱硫污泥驯化影响
|
摘要
实验研究了底物、接种污泥和微生物生长方式对猪场废水脱氮和沼气脱硫耦联污泥驯化及活性恢复的影响,以解决快速富集培养废水脱氮与沼气脱硫微生物的问题。研究发现,就脱氮脱硫均达到60%的时间而言,接种厌氧污泥反应器为9 d,比接种好氧污泥反应器(18 d)和不接种污泥加填料反应器(21 d)更短。以含氮含硫废水为底物驯化时,接种厌氧污泥更有利于脱氮脱硫污泥的驯化;而同为接种好氧污泥时,以含氮含硫废水为底物的驯化方式更有利于脱氮脱硫污泥的驯化。污泥活性恢复实验中,以含氮废水+沼气(H2S)为底物培养驯化的污泥,硫转化活性恢复所用的时间为15 d,比含氮含硫废水为底物驯化污泥的活性恢复时间更长。
The effect of substrate,inoculum and culturing mode on the acclimation of sludge of nitrogen removal from wastewater and hydrogen sulfide from biogas was investigated in the laboratory. The main research results show that the reactor inoculated with anaerobic sludge spent less time(9 d) to reach the removal rate of 60% for simultaneous removal of nitrogen and H2S than the reactor with anaerobic sludge reactor(18 d) and the reactor without sludge(21 d). Under the condition with wastewater containing nitrate and sulfide as substrate, the reactor inoculated with anaerobic sludge was more advantageous to the acclimation of sludge. Under the condition inoculated with aerobic sludge,the cultivating reacter with wastewater containing nitrate and sulfide as substrate was more advantageous to that with wastewater containing nitrate nitrogen and biogas containing H2S. In the experiment of recovering activity of sludge,recovering time of activity of the sludge acclimated from the cultivating mode with wastewater containing nitrate nitrogen and biogas containing H2S as substrate was 15 d,longer than that with wastewater containing nitrate and sulfide.
The effect of substrate,inoculum and culturing mode on the acclimation of sludge of nitrogen removal from wastewater and hydrogen sulfide from biogas was investigated in the laboratory. The main research results show that the reactor inoculated with anaerobic sludge spent less time(9 d) to reach the removal rate of 60% for simultaneous removal of nitrogen and H2S than the reactor with anaerobic sludge reactor(18 d) and the reactor without sludge(21 d). Under the condition with wastewater containing nitrate and sulfide as substrate, the reactor inoculated with anaerobic sludge was more advantageous to the acclimation of sludge. Under the condition inoculated with aerobic sludge,the cultivating reacter with wastewater containing nitrate and sulfide as substrate was more advantageous to that with wastewater containing nitrate nitrogen and biogas containing H2S. In the experiment of recovering activity of sludge,recovering time of activity of the sludge acclimated from the cultivating mode with wastewater containing nitrate nitrogen and biogas containing H2S as substrate was 15 d,longer than that with wastewater containing nitrate and sulfide.
引文
[1]Visser J.M.,Stefess G.C.,Kuenen J.G.Thiobacillus sp.w5,the dominant autotroph oxidizing sulfide to sulfur in a reator for aerobic treatment of sulfidic wastes.Antonie van Leeuwenhoek,1997,72(2):127-134
[2]Syed M.A.,Henshaw P.F.Effect of tube size on performance of a fixed-film tubular bioreactor for conversion of hydr ogen sulfide to elemental sulfur.Water Research,2003,37(8):1932-1938
[3]Waki M.,Yokoyama H.,Ogino A.,et al.Nitrogen removal from purified swine wastewater using biogas by semi-partitioned reactor.Bioresource Technology,2008,99(13):5335-5340
[4]Bernet N.,Béline F.Challenges and innovations on biological treatment of livestock effluents.Bioresource Technology,2009,100(22):5431-5436
[5]Bortone.G.Integrated anaerobic aerobic biological treatment for intensive swine production.Bioresource Technology,2009,100(22):5424-5430
[6]Yang Hong,Li Daotang,Zhu Zhangyu,et al.ABR-ICEAS system for piggery wastewater treatment.Journal of Shanghai Tongji University,2000,34(4):558-560
[7]Deng L.W.,Zheng P.,Chen Z.A.Anaerobic digestion and post-treatment of swine wastewate using IC-SBR process with bypass of raw wastewater.Process Biochemistry,2006,41(4):965-969
[8]Kim B.U.,Lee W.H.,Lee H.J.,et al.Ammonium nitrogen removal from slurry-type swine wastewater by pretreatment using struvite crystallization for nitrogen control of anaerobic digestion.Water Science and Technology,2004,49(5-6):215-222
[9]杨虹,李道棠,朱章玉,等.集约化养猪场冲栏水的达标处理.上海交通大学学报,2000,34(4):558-560Yang Hong,Li Daotang,Zhu Zhangyu,et al.ABR-ICEAS system for piggery wastewater treatment.Journal of Shanghai Jiaotong University,2000,34(4):558-560(in Chinese)
[10]Obaja D.,Mace S.,Costa J.,et al.Nitrification denitrification and biological phosphorus removal in piggery wastewater using sequencing batch reactor.Bioresource Technology,2003,87(1):103-111
[11]Deng L.W.,Chen H.J.,Chen Z.A.,et al.Process of simultaneous hydrogen sulfide removal from biogas and nitrogen removal from swine wastewater.Bioresource Technology,2009,100(23):5600-5608
[12]陈会娟,邓良伟,陈子爱.猪场废水脱氮与沼气脱硫耦联反应器的启动.环境科学学报,2008,28(8):1542-1548Chen H.J.,Deng L.W.,Chen Z.A.Start-up of a coupled reactor for nitrogen removal from swine wastewater and sulfide removal from biogas.Acta Scientiae Circumstantiae,2008,28(8):1542-1548(in Chinese)
[13]陈子爱,邓良伟,陈会娟,等.废水脱氮与沼气脱硫耦联菌株的驯化和分离.环境科学,2008,29(4):1099-1103Chen Z.A.,Deng L.W.,Chen H.J.,et al.Cultivation and isolation of bacteria coupling nitrogen removal from wastewater and hydrogen sulfide removal from biogas.Environmental Science,2008,29(4):1099-1103(in Chinese)
[14]孙竹,伍钧,蒲小东,等.高径比对猪场废水脱氮与沼气脱硫耦联反应器的影响.环境科学,2010,31(9):2214-2219Sun Z.,Wu J.,Pu X.D.,et al.Effect of the ratio of height to diameter on the performance of the simultaneous reactor for nitrogen removal from swine wastewater and sulfide removal from biogas.Environmental Science,2010,31(9):2214-2219(in Chinese)
[15]Zheng Dan,Deng Liangwei,Fan Zhanhui,et al.Influence of sand layer depth on partial nitritation as pretreatment of anaerobically digested swine wastewater prior to anammox.Bioresource Technology,2012,104:274-279
[16]王爱杰,杜大仲,任南琪.脱氮硫杆菌同步脱硫反硝化技术的关键因素研究.地球科学进展,2004,19(6):533-536Wang A.J.,Du D.Z.,Ren N.Q.Key factors affecting the process of simultaneous de-sulfide and de-nitrification.Advance in Earth Science,2004,19(6):533-536(in Chinese)
[17]国家环境保护总局.水和废水监测分析方法(第3版).北京:中国环境科学出版社,1989
[18]范彬,曲久辉,刘锁祥,等.饮用水中硝酸盐的脱除.环境污染治理技术与设备,2000,1(3):44-50Fan B.,Qu J.H.,Liu S.X.,et al.Removal of nitrate in drinking water.Techniques and Equipment for Environmental Pollution Control,2000,1(3):44-50(in Chinese)
[2]Syed M.A.,Henshaw P.F.Effect of tube size on performance of a fixed-film tubular bioreactor for conversion of hydr ogen sulfide to elemental sulfur.Water Research,2003,37(8):1932-1938
[3]Waki M.,Yokoyama H.,Ogino A.,et al.Nitrogen removal from purified swine wastewater using biogas by semi-partitioned reactor.Bioresource Technology,2008,99(13):5335-5340
[4]Bernet N.,Béline F.Challenges and innovations on biological treatment of livestock effluents.Bioresource Technology,2009,100(22):5431-5436
[5]Bortone.G.Integrated anaerobic aerobic biological treatment for intensive swine production.Bioresource Technology,2009,100(22):5424-5430
[6]Yang Hong,Li Daotang,Zhu Zhangyu,et al.ABR-ICEAS system for piggery wastewater treatment.Journal of Shanghai Tongji University,2000,34(4):558-560
[7]Deng L.W.,Zheng P.,Chen Z.A.Anaerobic digestion and post-treatment of swine wastewate using IC-SBR process with bypass of raw wastewater.Process Biochemistry,2006,41(4):965-969
[8]Kim B.U.,Lee W.H.,Lee H.J.,et al.Ammonium nitrogen removal from slurry-type swine wastewater by pretreatment using struvite crystallization for nitrogen control of anaerobic digestion.Water Science and Technology,2004,49(5-6):215-222
[9]杨虹,李道棠,朱章玉,等.集约化养猪场冲栏水的达标处理.上海交通大学学报,2000,34(4):558-560Yang Hong,Li Daotang,Zhu Zhangyu,et al.ABR-ICEAS system for piggery wastewater treatment.Journal of Shanghai Jiaotong University,2000,34(4):558-560(in Chinese)
[10]Obaja D.,Mace S.,Costa J.,et al.Nitrification denitrification and biological phosphorus removal in piggery wastewater using sequencing batch reactor.Bioresource Technology,2003,87(1):103-111
[11]Deng L.W.,Chen H.J.,Chen Z.A.,et al.Process of simultaneous hydrogen sulfide removal from biogas and nitrogen removal from swine wastewater.Bioresource Technology,2009,100(23):5600-5608
[12]陈会娟,邓良伟,陈子爱.猪场废水脱氮与沼气脱硫耦联反应器的启动.环境科学学报,2008,28(8):1542-1548Chen H.J.,Deng L.W.,Chen Z.A.Start-up of a coupled reactor for nitrogen removal from swine wastewater and sulfide removal from biogas.Acta Scientiae Circumstantiae,2008,28(8):1542-1548(in Chinese)
[13]陈子爱,邓良伟,陈会娟,等.废水脱氮与沼气脱硫耦联菌株的驯化和分离.环境科学,2008,29(4):1099-1103Chen Z.A.,Deng L.W.,Chen H.J.,et al.Cultivation and isolation of bacteria coupling nitrogen removal from wastewater and hydrogen sulfide removal from biogas.Environmental Science,2008,29(4):1099-1103(in Chinese)
[14]孙竹,伍钧,蒲小东,等.高径比对猪场废水脱氮与沼气脱硫耦联反应器的影响.环境科学,2010,31(9):2214-2219Sun Z.,Wu J.,Pu X.D.,et al.Effect of the ratio of height to diameter on the performance of the simultaneous reactor for nitrogen removal from swine wastewater and sulfide removal from biogas.Environmental Science,2010,31(9):2214-2219(in Chinese)
[15]Zheng Dan,Deng Liangwei,Fan Zhanhui,et al.Influence of sand layer depth on partial nitritation as pretreatment of anaerobically digested swine wastewater prior to anammox.Bioresource Technology,2012,104:274-279
[16]王爱杰,杜大仲,任南琪.脱氮硫杆菌同步脱硫反硝化技术的关键因素研究.地球科学进展,2004,19(6):533-536Wang A.J.,Du D.Z.,Ren N.Q.Key factors affecting the process of simultaneous de-sulfide and de-nitrification.Advance in Earth Science,2004,19(6):533-536(in Chinese)
[17]国家环境保护总局.水和废水监测分析方法(第3版).北京:中国环境科学出版社,1989
[18]范彬,曲久辉,刘锁祥,等.饮用水中硝酸盐的脱除.环境污染治理技术与设备,2000,1(3):44-50Fan B.,Qu J.H.,Liu S.X.,et al.Removal of nitrate in drinking water.Techniques and Equipment for Environmental Pollution Control,2000,1(3):44-50(in Chinese)
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |