品种间大豆脂肪氧化酶活性差异及β-胡萝卜素漂白性研究
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摘要
为了解东北地区大豆品种间大豆脂肪氧化酶(LOX)活性差异规律,整理适于东北地区种植的大豆种质资源307份(6份半野生品种,17份地方品种及4份国外品种,其余为栽培品种),统一种植,秋季收获后,测定各品种成熟籽粒脂肪氧化酶总活性。分析品种间脂肪氧化酶总活性分布规律、大豆脂肪氧化酶总活性与β-胡萝卜素漂白性之间关系。结果表明,307份大豆品种间脂肪氧化酶总活性差异极显著,平均值为21.68 U·mL~(-1),最小值为2.51 U·mL~(-1),最大值为42.38 U·mL~(-1)。根据脂肪氧化酶总活性差异,通过聚类分析将307份大豆品种分为3类。低活性品种占总量43.65%,脂肪氧化酶总活性平均值为15.25 U·mL~(-1);中等活性品种占总量43.97%,脂肪氧化酶活性平均值为24.64 U·mL~(-1);高活性品种占总量12.38%,脂肪氧化酶总活性平均值为33.87 U·mL~(-1)。脂肪氧化酶总活性与油分含量呈正相关,与蛋白含量呈负相关,与漂白性呈正相关。
In order to understand the difference in soybean lipoxygenase(LOX) activity among major soybean varieties for planting in Northeast China, 307 soybean varieties suitable to Northeast China(including six semi-wild varieties, 17 local varieties and four foreign varieties, the rest were cultivars) were planted. The LOX activity of mature seeds was measured, and the distribution of LOX activity and the relationship between soybean LOX activity and β-carotene bleaching were analyzed.The results showed that there were significant differences in the activities of LOX in 307 soybean varieties. The average LOX activity of the 307 soybean varieties studied was 21.68 U · mL~(-1), the minimum value was 2.51 U· mL~(-1), the maximum value was 42.38 U· mL~(-1), and the maximum value was10.07 times of the minimum value. According to the difference in LOX activity, 307 soybean varietieswere classified into three categories by cluster analysis. Low-activity varieties accounted for 43.65% of the total, and the average value of LOX activity was 15.25 U· mL~(-1). Medium active varieties accounted for 43.97% of the total, LOX activity averaged 24.64 U· mL~(-1); high active varieties accounted for 12.38%of the total, and LOX activity averaged 33.87 U· mL~(-1). LOX activity was positively correlated with oil content,negatively correlated with protein content, and positively correlated with β-carotene bleaching power.
In order to understand the difference in soybean lipoxygenase(LOX) activity among major soybean varieties for planting in Northeast China, 307 soybean varieties suitable to Northeast China(including six semi-wild varieties, 17 local varieties and four foreign varieties, the rest were cultivars) were planted. The LOX activity of mature seeds was measured, and the distribution of LOX activity and the relationship between soybean LOX activity and β-carotene bleaching were analyzed.The results showed that there were significant differences in the activities of LOX in 307 soybean varieties. The average LOX activity of the 307 soybean varieties studied was 21.68 U · mL~(-1), the minimum value was 2.51 U· mL~(-1), the maximum value was 42.38 U· mL~(-1), and the maximum value was10.07 times of the minimum value. According to the difference in LOX activity, 307 soybean varietieswere classified into three categories by cluster analysis. Low-activity varieties accounted for 43.65% of the total, and the average value of LOX activity was 15.25 U· mL~(-1). Medium active varieties accounted for 43.97% of the total, LOX activity averaged 24.64 U· mL~(-1); high active varieties accounted for 12.38%of the total, and LOX activity averaged 33.87 U· mL~(-1). LOX activity was positively correlated with oil content,negatively correlated with protein content, and positively correlated with β-carotene bleaching power.
引文
[1]Brash A R.Lipoxygenases:Occurrence,functions,catalysis,and acquisition of substrate[J].Journal of Biological Chemistry,1999,274(34):23679-23682.
[2]Axelrod B,Cheesbrough T M,Laakso S.Lipoxygenase from soybeans:EC1.13.11.12 Linoleate:Oxygen oxidoreductase[M].Pittsburgh:Academic Press,1981.
[3]Sessa D J,Rackis J J.Lipid-derived flavors of legume protein products[J].Journal of the American Oil Chemists'Society,1977,54(10):468-473.
[4]Moreira M A,Tavares S R,Ramos V,et al.Hexanal production and TBA number are reduced in soybean[Glycine max(L.)Merr.]seeds lacking lipoxygenase isozymes 2 and 3[J].Journal of Agricultural and Food Chemistry,1993,41(1):103-106.
[5]王若兰,李浩杰,孙中磊,等.Lox酶缺失大豆新品种耐储藏特性的研究[J].粮食储藏,2008(3):34-38.
[6]Parrish D J,Leopold A C.On the mechanism of aging in soybean seeds[J].Plant Physiology,1978,61(3):365-368.
[7]Feussner I,Kühn H,Wasternack C.Lipoxygenase-dependent degradation of storage lipids[J].Trends in Plant Science,2001,6(6):268-273.
[8]扎桑,卓嘎,徐东东,等.作物脂肪氧化酶的研究进展[J].大麦与谷类科学,2015(2):12-20.
[9]Saravitz D M,SiedowJ N.The differential expression of wound-Inducible lipoxygenase genes in soybean leaves[J].Plant Physiology,1996,110(1):287-299.
[10]Feussner I,Wasternack C.The lipoxygenase pathway[J].Annual Review of Plant Biology,2002,53(1):275-297.
[11]蔡琨,方云,夏咏梅.植物脂肪氧合酶的研究进展[J].现代化工,2003,23(S1):23-27.
[12]蔡燕,方云,孟君.大豆脂肪氧化酶催化亚油酸氢过氧化反应产物的漂白特性[J].大豆科学,2007(2):235-239.
[13]张福彦,陈锋,程仲杰,等.小麦TaLox-B等位变异对脂肪氧化酶活性和面粉色泽的影响[J].中国农业科学,2017,50(8):1370-1377.
[14]张雪丹.大豆脂肪氧化酶的鉴定、纯化及其漂白β-胡萝卜素的酶学特性研究[D].南京:南京农业大学,2008.
[15]Hildebrand D F,Hymowitz T.Two soybean genotypes lacking lipoxygen-ase-1[J].Journal of the American Oil Chemists'Society,1981,58:583-586.
[16]傅翠珍,徐文英.中国大豆脂肪氧化酶缺失种质多样性鉴定研究[J].中国农业科学,1997,30(1):44-51.
[17]Pfeiffer T W,Hildebrand D F,Tekrony D M.Agronomic performance of soybean lipoxygenase isolines[J].Crop Science,1992,32(2):357-362.
[18]谢雄泽,藕冉,王磊,等.大豆脂氧酶与7S球蛋白亚基双缺失株系的主要品质性状分析[J].中国油料作物学报,2016,38(6):764-770.
[19]陈影,张晟瑞,王岚,等.野生和栽培大豆种质油脂组成特点及其与演化的关系[J].作物学报,2019(3):1-14.
[20]宋晓昆,张颖君,闫龙,等.大豆脂肪酸组份相关、变异特点分析[J].华北农学报,2010,25(S2):68-73.
[2]Axelrod B,Cheesbrough T M,Laakso S.Lipoxygenase from soybeans:EC1.13.11.12 Linoleate:Oxygen oxidoreductase[M].Pittsburgh:Academic Press,1981.
[3]Sessa D J,Rackis J J.Lipid-derived flavors of legume protein products[J].Journal of the American Oil Chemists'Society,1977,54(10):468-473.
[4]Moreira M A,Tavares S R,Ramos V,et al.Hexanal production and TBA number are reduced in soybean[Glycine max(L.)Merr.]seeds lacking lipoxygenase isozymes 2 and 3[J].Journal of Agricultural and Food Chemistry,1993,41(1):103-106.
[5]王若兰,李浩杰,孙中磊,等.Lox酶缺失大豆新品种耐储藏特性的研究[J].粮食储藏,2008(3):34-38.
[6]Parrish D J,Leopold A C.On the mechanism of aging in soybean seeds[J].Plant Physiology,1978,61(3):365-368.
[7]Feussner I,Kühn H,Wasternack C.Lipoxygenase-dependent degradation of storage lipids[J].Trends in Plant Science,2001,6(6):268-273.
[8]扎桑,卓嘎,徐东东,等.作物脂肪氧化酶的研究进展[J].大麦与谷类科学,2015(2):12-20.
[9]Saravitz D M,SiedowJ N.The differential expression of wound-Inducible lipoxygenase genes in soybean leaves[J].Plant Physiology,1996,110(1):287-299.
[10]Feussner I,Wasternack C.The lipoxygenase pathway[J].Annual Review of Plant Biology,2002,53(1):275-297.
[11]蔡琨,方云,夏咏梅.植物脂肪氧合酶的研究进展[J].现代化工,2003,23(S1):23-27.
[12]蔡燕,方云,孟君.大豆脂肪氧化酶催化亚油酸氢过氧化反应产物的漂白特性[J].大豆科学,2007(2):235-239.
[13]张福彦,陈锋,程仲杰,等.小麦TaLox-B等位变异对脂肪氧化酶活性和面粉色泽的影响[J].中国农业科学,2017,50(8):1370-1377.
[14]张雪丹.大豆脂肪氧化酶的鉴定、纯化及其漂白β-胡萝卜素的酶学特性研究[D].南京:南京农业大学,2008.
[15]Hildebrand D F,Hymowitz T.Two soybean genotypes lacking lipoxygen-ase-1[J].Journal of the American Oil Chemists'Society,1981,58:583-586.
[16]傅翠珍,徐文英.中国大豆脂肪氧化酶缺失种质多样性鉴定研究[J].中国农业科学,1997,30(1):44-51.
[17]Pfeiffer T W,Hildebrand D F,Tekrony D M.Agronomic performance of soybean lipoxygenase isolines[J].Crop Science,1992,32(2):357-362.
[18]谢雄泽,藕冉,王磊,等.大豆脂氧酶与7S球蛋白亚基双缺失株系的主要品质性状分析[J].中国油料作物学报,2016,38(6):764-770.
[19]陈影,张晟瑞,王岚,等.野生和栽培大豆种质油脂组成特点及其与演化的关系[J].作物学报,2019(3):1-14.
[20]宋晓昆,张颖君,闫龙,等.大豆脂肪酸组份相关、变异特点分析[J].华北农学报,2010,25(S2):68-73.
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