牙周炎患者血清中内脂素和PGE2水平检测及其与牙周炎活动性的关系
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摘要
目的:检测牙周炎患者血清中内脂素、前列腺素E2(PGE2)和白细胞介素1β(IL-1β)的水平,探讨内脂素和PGE2在慢性牙周炎发病中的作用及其对于牙周炎活动性评估的临床意义。方法:选取79例慢性牙周炎患者作为慢性牙周炎组,根据慢性牙周炎活动期的诊断标准将患者分为慢性牙周炎活动期组(33例)和慢性牙周炎静止期组(46例);另选取健康志愿者25例作为健康对照组。收集各组研究对象的血清,采用ELISA法定量检测各组研究对象血清中内脂素、PGE2和IL-1β水平,采用Spearman分析和偏相关分析检测各组研究对象血清中内脂素水平与PGE2、IL-1β水平的相关性。结果:慢性牙周炎组患者血清中内脂素、PGE2和IL-1β水平均明显高于健康对照组(P<0.05);慢性牙周炎活动期组患者血清中内脂素、PGE2和IL-1β水平高于慢性牙周炎静止期组(P<0.05)。相关性分析,慢性牙周炎组患者血清中内脂素与IL-1β水平(r=0.68,P<0.05)、IL-1β与PGE2水平(r=0.79,P<0.05)、内脂素与PGE2水平(r=0.63,P<0.05)呈正相关关系;患者血清中内脂素水平越高,PGE2水平越高。慢性牙周炎活动期组患者血清中内脂素与PGE2水平呈显著正相关关系(r=0.78,P<0.05),而慢性牙周炎静止期组患者血清中内脂素与PGE2水平则呈低度正相关关系(r=0.45,P<0.05)。结论:内脂素和PGE2可能共同参与了牙周炎的发生发展过程。血清中内脂素和PGE2水平升高可能预示着牙周炎处于活动期。
Objective:To detect the levels of visfatin,prostaglandin E2(PGE2)and interleukin-1β(IL-1β)in serum of the chronic periodontitis patients,and to investigate the role of the above three factors in the pathogenesis of chronic periodontitis and their clinical significances in the assessment of activity of chronic periodontitis.Methods:A total of 79 cases of chronic periodontitis patients(including active and resting stages)were selected.The chronic periodontitis patients were divided into active stage of chronic periodontitis group(n=33)and resting stage of chronic periodontitis group(n=46)according to the diagnostic criteria for chronic periodontitis activity.A total of 25 cases of healthy volunteers were also selected as healthy control group.The serum of all cases were collected,then the serum levels of visfatin,PGE2 and IL-1βof the subjects in various groups were detected by ELISA method.The relationships between the serum levels of visfatin,PGE2 and IL-1βwere analyzed with Spearman and partial correlation analysis.Results:The levels of serum visfatin,PGE2 and IL-1βof the patients in chronic periodontitis group were significantly higher than those in healthy control group(P<0.05).The levels of serum visfatin,PGE2 and IL-1βof the patients in active stage of chronic periodontitis group were significantly higher than those in resting stage of chronic periodontitis group(P <0.05).The correlative analysis results demonstrated that the serum IL-1βlevel had positive relationships with the serum levels of visfatin and PGE2 of the patients in chronic periodontitis group(r=0.68,P<0.05;r=0.79,P<0.05).The serum visfatin level had positive relationships with the serum PGE2 level of the patients in chronic periodontitis group(r=0.63,P<0.05);the higher the serum visfatin level was,the higher the serum PGE2 level was.The serum visfatin level had significantly positive relationship with the serum PGE2 level of the patients in active stage of chronic periodontitis group(r=0.78,P<0.05).The serum levels of visfatin and PGE2 of the patients in resting stage of chronic periodontitis group had positive correlation at a low level(r=0.45,P<0.05).Conclusion:Visfatin and PGE2 may be involved in the pathogenesis of chronic periodontitis.The increasing of serum visfatin and PGE2 levels may predict the active stage of chronic periodontitis.
Objective:To detect the levels of visfatin,prostaglandin E2(PGE2)and interleukin-1β(IL-1β)in serum of the chronic periodontitis patients,and to investigate the role of the above three factors in the pathogenesis of chronic periodontitis and their clinical significances in the assessment of activity of chronic periodontitis.Methods:A total of 79 cases of chronic periodontitis patients(including active and resting stages)were selected.The chronic periodontitis patients were divided into active stage of chronic periodontitis group(n=33)and resting stage of chronic periodontitis group(n=46)according to the diagnostic criteria for chronic periodontitis activity.A total of 25 cases of healthy volunteers were also selected as healthy control group.The serum of all cases were collected,then the serum levels of visfatin,PGE2 and IL-1βof the subjects in various groups were detected by ELISA method.The relationships between the serum levels of visfatin,PGE2 and IL-1βwere analyzed with Spearman and partial correlation analysis.Results:The levels of serum visfatin,PGE2 and IL-1βof the patients in chronic periodontitis group were significantly higher than those in healthy control group(P<0.05).The levels of serum visfatin,PGE2 and IL-1βof the patients in active stage of chronic periodontitis group were significantly higher than those in resting stage of chronic periodontitis group(P <0.05).The correlative analysis results demonstrated that the serum IL-1βlevel had positive relationships with the serum levels of visfatin and PGE2 of the patients in chronic periodontitis group(r=0.68,P<0.05;r=0.79,P<0.05).The serum visfatin level had positive relationships with the serum PGE2 level of the patients in chronic periodontitis group(r=0.63,P<0.05);the higher the serum visfatin level was,the higher the serum PGE2 level was.The serum visfatin level had significantly positive relationship with the serum PGE2 level of the patients in active stage of chronic periodontitis group(r=0.78,P<0.05).The serum levels of visfatin and PGE2 of the patients in resting stage of chronic periodontitis group had positive correlation at a low level(r=0.45,P<0.05).Conclusion:Visfatin and PGE2 may be involved in the pathogenesis of chronic periodontitis.The increasing of serum visfatin and PGE2 levels may predict the active stage of chronic periodontitis.
引文
[1]Sung MS,Lee EG,Jeon HS,et al.Quercetin inhibits IL-1β-induced proliferation and production of MMPs,COX-2,and PGE2by rheumatoid synovial fibroblast[J].Inflammation,2012,35(4):1585-1594.
[2]Baser U,Oztekin G,Ademoglu E,et al.Is the severity of periodontitis related to gingival crevicular fluid and serum highsensitivity C-reactive protein concentrations?[J].Clin Lab,2014,60(10):1653-1658.
[3]Sánchez GA,Miozza VA,Delgado A,et al.Salivary IL-1βand PGE2as biomarkers of periodontal status,before and after periodontal treatment[J].J Clin Periodontol,2013,40(12):1112-1117.
[4]Raghavendra NM,Pradeep AR,Kathariya R,et al.Effect of non surgical periodontal therapy on gingival crevicular fluid and serum visfatin concentration in periodontal health and disease[J].Dis Markers,2012,32(6):383-388.
[5]Abolfazli N,Jabali S,Saber FS,et al.Effect of non-surgical periodontal therapy on serum and salivary concentrations of visfatin in patients with chronic periodontitis[J].J Dent Res Dent Clin Dent Prospects,2015,9(1):11-17.
[6]Chen Z,Zhao GH,Zhang YK,et al.Research on the correlation of diabetes mellitus complicated with osteoporosis with lipid metabolism,adipokines and inflammatory factors and its regression analysis[J].Eur Rev Med Pharmacol Sci,2017,21(17):3900-3905.
[7]Pradeep AR,Raghavendra NM,Prasad MV,et al.Gingival crevicular fluid and serum visfatin concentration:their relationship in periodontal health and disease[J].J Periodontol,2011,82(9):1314.
[8]胥丹妮,林晓萍.脂肪因子对牙周炎症组织影响机制的研究[J].口腔医学,2017,37(10):932-936.
[9]Nokhbehsaim M,Eick S,Nogueira AV,et al.Stimulation of MMP-1and CCL2by NAMPT in PDL cells[J].Mediators Inflam,2013,2013(4):437123.
[10]Moschen AR,Kaser A,Enrich B,et al.Visfatin,an adipocytokine with proinflammatory and immunomodulating properties[J].J Immunol,2007,178(3):1748-1758.
[11]戴兵,徐俐,金海东,等.橄榄苦苷对IL-1β诱导的大鼠软骨细胞损伤的作用及机制研究[J].中国病理生理杂志,2015,31(9):1667-1672.
[12]Murayama R,Kobayashi M,Takeshita A,et al.MAPKs,activator protein-1and nuclear factor-κB mediate production of interleukin-1β-stimulated cytokines,prostaglandin E2 and MMP-1in human periodontal ligament cells[J].J Periodont Res,2011,46(5):568.
[13]Gursoy UK,K9n9nen E,Pradhan-Palikhe P,et al.Salivary MMP-8,TIMP-1,and ICTP as markers of advanced periodontitis[J].J Clin Periodontol,2010,37(6):487-493.
[14]庞显伦,杨健,秦昊.人工关节炎性介质对滑膜细胞基质金属蛋白酶-9表达的影响[J].中华实验外科杂志,2016,33(4):1052-1054.
[15]Huang Q,Lan F,Wang X,et al.IL-1β-induced activation of p38 promotes metastasis in gastric adenocarcinoma via upregulation of AP-1/c-fos,MMP2 and MMP9[J].Mol Cancer,2014,13(1):18.
[16]Adya R,Tan BK,Chen J,et al.Nuclear factor-kappaB induction by visfatin in human vascular endothelial cells:Its role in MMP-2/9 production and activation[J].Diabetes Care,2008,31(4):758-760.
[17]张晔,张连阳,孙士锦,等.脂多糖作用下血管内皮钙黏蛋白经网格蛋白和微囊介导胞吞后的亚细胞分布差异[J].解放军医学杂志,2016,41(1):22-25.
[2]Baser U,Oztekin G,Ademoglu E,et al.Is the severity of periodontitis related to gingival crevicular fluid and serum highsensitivity C-reactive protein concentrations?[J].Clin Lab,2014,60(10):1653-1658.
[3]Sánchez GA,Miozza VA,Delgado A,et al.Salivary IL-1βand PGE2as biomarkers of periodontal status,before and after periodontal treatment[J].J Clin Periodontol,2013,40(12):1112-1117.
[4]Raghavendra NM,Pradeep AR,Kathariya R,et al.Effect of non surgical periodontal therapy on gingival crevicular fluid and serum visfatin concentration in periodontal health and disease[J].Dis Markers,2012,32(6):383-388.
[5]Abolfazli N,Jabali S,Saber FS,et al.Effect of non-surgical periodontal therapy on serum and salivary concentrations of visfatin in patients with chronic periodontitis[J].J Dent Res Dent Clin Dent Prospects,2015,9(1):11-17.
[6]Chen Z,Zhao GH,Zhang YK,et al.Research on the correlation of diabetes mellitus complicated with osteoporosis with lipid metabolism,adipokines and inflammatory factors and its regression analysis[J].Eur Rev Med Pharmacol Sci,2017,21(17):3900-3905.
[7]Pradeep AR,Raghavendra NM,Prasad MV,et al.Gingival crevicular fluid and serum visfatin concentration:their relationship in periodontal health and disease[J].J Periodontol,2011,82(9):1314.
[8]胥丹妮,林晓萍.脂肪因子对牙周炎症组织影响机制的研究[J].口腔医学,2017,37(10):932-936.
[9]Nokhbehsaim M,Eick S,Nogueira AV,et al.Stimulation of MMP-1and CCL2by NAMPT in PDL cells[J].Mediators Inflam,2013,2013(4):437123.
[10]Moschen AR,Kaser A,Enrich B,et al.Visfatin,an adipocytokine with proinflammatory and immunomodulating properties[J].J Immunol,2007,178(3):1748-1758.
[11]戴兵,徐俐,金海东,等.橄榄苦苷对IL-1β诱导的大鼠软骨细胞损伤的作用及机制研究[J].中国病理生理杂志,2015,31(9):1667-1672.
[12]Murayama R,Kobayashi M,Takeshita A,et al.MAPKs,activator protein-1and nuclear factor-κB mediate production of interleukin-1β-stimulated cytokines,prostaglandin E2 and MMP-1in human periodontal ligament cells[J].J Periodont Res,2011,46(5):568.
[13]Gursoy UK,K9n9nen E,Pradhan-Palikhe P,et al.Salivary MMP-8,TIMP-1,and ICTP as markers of advanced periodontitis[J].J Clin Periodontol,2010,37(6):487-493.
[14]庞显伦,杨健,秦昊.人工关节炎性介质对滑膜细胞基质金属蛋白酶-9表达的影响[J].中华实验外科杂志,2016,33(4):1052-1054.
[15]Huang Q,Lan F,Wang X,et al.IL-1β-induced activation of p38 promotes metastasis in gastric adenocarcinoma via upregulation of AP-1/c-fos,MMP2 and MMP9[J].Mol Cancer,2014,13(1):18.
[16]Adya R,Tan BK,Chen J,et al.Nuclear factor-kappaB induction by visfatin in human vascular endothelial cells:Its role in MMP-2/9 production and activation[J].Diabetes Care,2008,31(4):758-760.
[17]张晔,张连阳,孙士锦,等.脂多糖作用下血管内皮钙黏蛋白经网格蛋白和微囊介导胞吞后的亚细胞分布差异[J].解放军医学杂志,2016,41(1):22-25.