香蕉与B基因组相关的SCAR标记研究
|
摘要
香蕉(Musa L.)是由2个二倍体野生种Musa acuminata Colla(AA基因型)和Musa balbisiana Colla(BB基因型)种内或种间杂交进化而来,其B基因组中带有重要的优良基因。利用与香蕉B基因组相关的gypsy-IRAP分子标记,成功开发了一对SCAR引物,适用于鉴定尖叶蕉(AAw)、长梗蕉(BB)、香牙蕉(AAA)、贡蕉(AAcv)、大蕉、粉蕉(ABB)、粉大蕉(ABB)、龙牙蕉(AAB)以及四倍体香蕉(AAAB)等是否含有B基因组。
The banana cultivars are originated from intra-and interspecific hybridization of two wild diploid species:Musa acuminate Colla and Musa balbisiana Colla,contributing the A and B genomes,respectively. The B genome contains important and good genes. A sequence characterized amplified region(SCAR)marker was successfully developed from the inter-retrotransposon amplified polymorphism(IRAP)marker named gypsy-IRAP related to banana B genome,and it was found to be able to identificate B genome of banana cultivars with different genotypes such as AAw,BB,AAcv,ABB,AAB,AAAB.
The banana cultivars are originated from intra-and interspecific hybridization of two wild diploid species:Musa acuminate Colla and Musa balbisiana Colla,contributing the A and B genomes,respectively. The B genome contains important and good genes. A sequence characterized amplified region(SCAR)marker was successfully developed from the inter-retrotransposon amplified polymorphism(IRAP)marker named gypsy-IRAP related to banana B genome,and it was found to be able to identificate B genome of banana cultivars with different genotypes such as AAw,BB,AAcv,ABB,AAB,AAAB.
引文
BretóM P,Ruiz C,Pina J A,Asíns M J.2001.The diversification of citrus clementina hort.ex tan.,a vegetatively propagated crop species.Molecular Phylogenetics and Evolution,21:285-293.
Cerqueira R C,Silva S D O,Medina V M.2002.Post-harvest fruit characteristics of different banana(Musa spp.)genotypes.Revista Brasileira De Fruticultura,24(3):654-657.
ChristelováP,Valárik M,H?ibováE,de Langhe E,Dole?el J.2011.A multi gene sequence-based phylogeny of the Musaceae(banana)family.BMC Evolutionary Biology,11(1):1-13.
Creste S,Neto A T,Vencovsky R,Silva S D O,Figueira A.2004.Genetic diversity of Musa diploid and triploid accessions from the Brazilian banana breeding program estimated by microsatellite markers.Genet Resources&Crop Evolution,51(7):723-733.
Davey M W,Gudimella R,Harikrishna J A,Sin L W,Khalid N,Keulemans J.2013.A draft Musa balbisiana genome sequence for molecular genetics in polyploid,inter-and intra-specific Musa hybrids.BMC Genomics,14:683-703.
De Langhe E,Pillay M,Tenkouano A,Swennen R.2005.Integrating morphological and molecular taxonomy in Musa:the African plantains(Musa spp.AAB group).Plant Systematics and Evolution,255:225-236.
D'Hont A,Denoeud F,Aury J M,Baurens F C,Carreel F,Garsmeur O,Noel B,Bocs S,Droc G,Rouard M,Da Silva C,Jabbari K,Cardi C,Poulain J,Souquet M,Labadie K,Jourda C,LengelléJ,Rodier-Goud M,Alberti A,Bernard M,Correa M,Ayyampalayam S,Mckain M R,Leebens-Mack J,Burgess D,Freeling M,Mbéguié-A-MbéguiéD,Chabannes M,Wicker T,Panaud O,Barbosa J,Hribova E,Heslop-Harrison P,Habas R,Rivallan R,Francois P,Poiron C,Kilian A,Burthia D,Jenny C,Bakry F,Brown S,Guignon V,Kema G,Dita M,Waalwijk C,Joseph S,Dievart A,Jaillon O,Leclercq J,Argout X,Lyons E,Almeida A,Jeridi M,Dolezel J,Roux N,Risterucci A M,Weissenbach J,Ruiz M,Glaszmann J C,Quétier F,Yahiaoui N,Wincker P.2012.The banana(Musa acuminata)genome and the evolution of monocotyledonous plants.Nature,488:213-217.
Doyle J J.1991.DNA protocols for plants-CTAB total DNA isolation//Hewitt GM,Johnston A.Molecular techniques in taxonomy.Berlin,Springer:283-293.
Du Xiao-yun,Luo Zheng-rong.2006.Establishment of the inter-retrotransposon amplified polymorphism(IRAP)reaction system and construction of fingerprint in some Diospyros spp.Journal of Agricultural Biotechnology,14(6):931-936.(in Chinese)杜晓云,罗正荣.2006.部分柿属植物IRAP反应体系的建立和指纹图谱构建.农业生物技术学报,14(6):931-936.
Du Xiao-yun,Zhang Qing-lin,Li Bao,Leng Ping,Zhang Ming-de,Huang Jian-min,Luo Zheng-rong.2011.Mutations identification based on IRAPanalysis in Diospyros kaki cv.Damopan.Journal of Fruit Science,28(2):257-262.(in Chinese)杜晓云,张青林,李宝,冷平,张明德,黄建民,罗正荣.2011.基于IRAP技术的磨盘柿变异单株鉴定.果树学报,28(2):257-262.
Horry J P,Dolezel J,Dolezelova M,Lysak M A.1998.Do natural A×B tetraploid bananas exist.INFOMUSA,7:5-6.
Jenny C,Carreel F,Bakry F.1997.Revision on banana taxonomy:“Klue Tiparot”(Musa sp.)reclassified as a triploid.Fruits,52(2):89-91.
Huang Jian-chang,Xiao Yan,Zhao Chun-xiang.1999.Some physiological responses of banana to water stress.Journal of Zhongkai Agrotechnical College,12(4):40-42.(in Chinese)黄建昌,肖艳,赵春香.1999.香蕉对水分胁迫的生理反应.仲恺农业技术学院学报,12(4):40-42.
Kalendar R,Grob T,Regina M,Suoniemi A,Schulman A H.1999.IRAP and REMAP:two new retrotransposon based DNA fingerprinting techniques.Theoretical and Applied Gebetics,98:704-711.
Leigh F,Kalendar R,Lea V,Lee D,Donini P,Schulman A.2003.Comparison of the utility of barley retrotransposon families for genetic analysis by molecular marker techniques.Mol Gen Genamies,269:464-474.
Li Bao-rong,Zhang Xiang-ping.2002.Name confusions of the introduced banana varieties in China and countermeasures.Tropical Agriculture Science,22(2):35-38.(in Chinese)李宝荣,张向平.2002.当前我国香蕉引进品种的名称混乱问题及其对策.热带农业科学,22(2):35-38.
Li Weiming,Chen Jingjing,Duan Yajie,Pang Zhencai,Sun Dequan,Hu Yulin,Hu Huigang,Xie Jianghui,Chen Wenna.2018.Research advances on taxonomy,geo-distribution and molecular phylogenetics of wild banana germplasm resources.Acta Horticulturae Sinica,45(9):1675-1687.(in Chinese)李伟明,陈晶晶,段雅婕,庞振才,孙德权,胡玉林,胡会刚,谢江辉,陈文娜.2018.香蕉野生种质资源的分类、分布和分子系统发育研究进展.园艺学报,45(9):1675-1687.
LüShun,Ren Yi,Wang Fang,Hu Gui-bing,Hung Bing-zhi,Liu Wen-qing,He Jian-qi,Liu Jian-ping,Zeng Li-sha,Zhou Jian-kun,Mai Jing-yu,Zhang Ke-heng.2018.Ploidy identification of 169 Musa germplasms by flow cytometry.Journal of Fruit Science,35(6):668-684.(in Chinese)吕顺,任毅,王芳,胡桂兵,黄秉智,刘文清,何建齐,刘建平,曾莉莎,周建坤,麦景郁,张珂恒.2018.利用流式细胞术快速鉴定169份香蕉种质资源的染色体倍性.果树学报,35(6):668-684.
Mabonga L,Pillay M.2017.SCAR marker for the a genome of bananas(Musa spp.L.)supports lack of differentiation between the A and B Genomes.Journal of Agricultural Science,9(6):64-73.
Manninen O,Kalendar R,Robinson J,Schulman A H.2000.Aplication of BARE-1 retrotransposon markers to map a major resistance gene for net blotch in barley.Molecular and General Genetics,264(3):325-334.
Nair A S,Teo C H,Schwarzacher T,Harrison P H.2005.Genome classification of banana cultivars from South India using IRAP markers.Euphytica,144:285-290.
Ning S P,Xu L B,Lu Y,Huang B Z,Ge X J.2007.Genome composition and genetic diversity of Musa germplasm from China revealed by PCR-RFLP and SSR markers.Scientia Horticulturae,114(4):281-288.
Nwakanma D C,Pillay M,Okoli B E,Tenkouano A.2003.PCR-RFLP of the ribosomal DNA internal transcribed spacers(ITS)provides markers for the A and B genomes in Musa L.Theoretical and Applied Genetics,108:154-159.
Onguso J M,Kahangi E M,Ndiritu D W,Mizutani F.2004.Genetic characterization of cultivated bananas and plantains in Kenya by RAPD markers.Scientia Horticulturae,99(1):9-20.
Pachuau L,Atom A D,Thangjam R.2014.Genome classification of Musa cultivars from Northeast India as revealed by ITS and IRAP markers.Appl Biochem Biotechnol,172:3939-3948.
Paran I,Michelmore R W.1993.Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce.Theor Appl Genet,85:985-993.
Pillay M,Nwakanma D C,Tenkouano A.2000.Identification of RAPD markers linked to A and B genome sequences in Musa L.Genome,43:763-767.
Pillay M,Ogundiwin E,Tenkouano A,Dolezel J.2006.Ploidyandgenome composition of Musa germplasm at the International Institute of Tropical Agriculture(IITA).Afr J Biotechnol,5:1224-1232.
Ramadass R,Sheriff M M.1993.Effect of moisture stress on the yield and yield components in banana as influenced by their genome and ploidy.Madras Agricultural Journal,80(3):130-133.
Robinson J C.1996.Bananas and plantains:crop production science in horticulture No.5.Wallingford,UK:CAB International:1-33.
Simmonds N W,Shepherd K.1955.The taxonomy and origins of the cultivated bananas.Botanical Journal of the Linnean Society,55:302-312.
Tang Dong-lan,Hu Yan,Gong Bang-chu,Zhang Qing-lin,Luo Zheng-rong.2016.Study on the hereditary variation of Jinzaoshi persimmon(Diospyros sp.)seedling progeny and the reproduction characteristics analyses based on SCoT and IRAP markers.Journal of Fruit Science,33(8):934-942.(in Chinese)唐冬兰,胡燕,龚榜初,张青林,罗正荣.2016.基于SCoT与IRAP标记的金枣柿实生后代遗传变异分析及其生殖特性.果树学报,33(8):934-942.
Teo C H,Tan S H,Ho C L,Faridah Q Z,Othman Y R,Heslop-Harrison J S,Kalendar R,Schulman A H.2005.Genome constitution and classification using retrotransposon-based markers in the orphan crop banana.Journal of Plant Biology,48(1):96-105.
Vakili N G.1967.The experimental formation of polyploidy and its effect in the genus Musa.Am J Bot,54:24-36.
Wang J Y,Zheng L S,Huang B Z,Liu W L,Wu Y T.2010.Development,characterization,and variability analysis of microsatellites from a commercial cultivar of Musa acuminata.Genetic Resources&Crop Evolution,57(4):553-563.
Wang Zhengxun,Lin Zhaoping,Pan Kunqing.1994.Cytogenetical studies in Musa(Eumusa).Acta Genetica Sinica,21(6):453-462.(in Chinese)王正询,林兆平,潘坤清.1994.蕉类的细胞遗传学研究.遗传学报,21(6):453-462.
Wei W,Fan Z Q,Chen J Y,Kuang J F,Lu W J,Shan W.2017.A banana PHD-type transcription factor MaPHD1 represses a cell wall-degradation gene Ma XTH6 during fruit ripening.Horticultural Plant Journal,3(5):190-198.
Wong C,Kiew R G,Set O,Lee S K,Gan Y Y.2002.Assessment of validity of the sections in Musa(Musaceae)using AFLP.Annals of Botany,90(2):231-238.
Cerqueira R C,Silva S D O,Medina V M.2002.Post-harvest fruit characteristics of different banana(Musa spp.)genotypes.Revista Brasileira De Fruticultura,24(3):654-657.
ChristelováP,Valárik M,H?ibováE,de Langhe E,Dole?el J.2011.A multi gene sequence-based phylogeny of the Musaceae(banana)family.BMC Evolutionary Biology,11(1):1-13.
Creste S,Neto A T,Vencovsky R,Silva S D O,Figueira A.2004.Genetic diversity of Musa diploid and triploid accessions from the Brazilian banana breeding program estimated by microsatellite markers.Genet Resources&Crop Evolution,51(7):723-733.
Davey M W,Gudimella R,Harikrishna J A,Sin L W,Khalid N,Keulemans J.2013.A draft Musa balbisiana genome sequence for molecular genetics in polyploid,inter-and intra-specific Musa hybrids.BMC Genomics,14:683-703.
De Langhe E,Pillay M,Tenkouano A,Swennen R.2005.Integrating morphological and molecular taxonomy in Musa:the African plantains(Musa spp.AAB group).Plant Systematics and Evolution,255:225-236.
D'Hont A,Denoeud F,Aury J M,Baurens F C,Carreel F,Garsmeur O,Noel B,Bocs S,Droc G,Rouard M,Da Silva C,Jabbari K,Cardi C,Poulain J,Souquet M,Labadie K,Jourda C,LengelléJ,Rodier-Goud M,Alberti A,Bernard M,Correa M,Ayyampalayam S,Mckain M R,Leebens-Mack J,Burgess D,Freeling M,Mbéguié-A-MbéguiéD,Chabannes M,Wicker T,Panaud O,Barbosa J,Hribova E,Heslop-Harrison P,Habas R,Rivallan R,Francois P,Poiron C,Kilian A,Burthia D,Jenny C,Bakry F,Brown S,Guignon V,Kema G,Dita M,Waalwijk C,Joseph S,Dievart A,Jaillon O,Leclercq J,Argout X,Lyons E,Almeida A,Jeridi M,Dolezel J,Roux N,Risterucci A M,Weissenbach J,Ruiz M,Glaszmann J C,Quétier F,Yahiaoui N,Wincker P.2012.The banana(Musa acuminata)genome and the evolution of monocotyledonous plants.Nature,488:213-217.
Doyle J J.1991.DNA protocols for plants-CTAB total DNA isolation//Hewitt GM,Johnston A.Molecular techniques in taxonomy.Berlin,Springer:283-293.
Du Xiao-yun,Luo Zheng-rong.2006.Establishment of the inter-retrotransposon amplified polymorphism(IRAP)reaction system and construction of fingerprint in some Diospyros spp.Journal of Agricultural Biotechnology,14(6):931-936.(in Chinese)杜晓云,罗正荣.2006.部分柿属植物IRAP反应体系的建立和指纹图谱构建.农业生物技术学报,14(6):931-936.
Du Xiao-yun,Zhang Qing-lin,Li Bao,Leng Ping,Zhang Ming-de,Huang Jian-min,Luo Zheng-rong.2011.Mutations identification based on IRAPanalysis in Diospyros kaki cv.Damopan.Journal of Fruit Science,28(2):257-262.(in Chinese)杜晓云,张青林,李宝,冷平,张明德,黄建民,罗正荣.2011.基于IRAP技术的磨盘柿变异单株鉴定.果树学报,28(2):257-262.
Horry J P,Dolezel J,Dolezelova M,Lysak M A.1998.Do natural A×B tetraploid bananas exist.INFOMUSA,7:5-6.
Jenny C,Carreel F,Bakry F.1997.Revision on banana taxonomy:“Klue Tiparot”(Musa sp.)reclassified as a triploid.Fruits,52(2):89-91.
Huang Jian-chang,Xiao Yan,Zhao Chun-xiang.1999.Some physiological responses of banana to water stress.Journal of Zhongkai Agrotechnical College,12(4):40-42.(in Chinese)黄建昌,肖艳,赵春香.1999.香蕉对水分胁迫的生理反应.仲恺农业技术学院学报,12(4):40-42.
Kalendar R,Grob T,Regina M,Suoniemi A,Schulman A H.1999.IRAP and REMAP:two new retrotransposon based DNA fingerprinting techniques.Theoretical and Applied Gebetics,98:704-711.
Leigh F,Kalendar R,Lea V,Lee D,Donini P,Schulman A.2003.Comparison of the utility of barley retrotransposon families for genetic analysis by molecular marker techniques.Mol Gen Genamies,269:464-474.
Li Bao-rong,Zhang Xiang-ping.2002.Name confusions of the introduced banana varieties in China and countermeasures.Tropical Agriculture Science,22(2):35-38.(in Chinese)李宝荣,张向平.2002.当前我国香蕉引进品种的名称混乱问题及其对策.热带农业科学,22(2):35-38.
Li Weiming,Chen Jingjing,Duan Yajie,Pang Zhencai,Sun Dequan,Hu Yulin,Hu Huigang,Xie Jianghui,Chen Wenna.2018.Research advances on taxonomy,geo-distribution and molecular phylogenetics of wild banana germplasm resources.Acta Horticulturae Sinica,45(9):1675-1687.(in Chinese)李伟明,陈晶晶,段雅婕,庞振才,孙德权,胡玉林,胡会刚,谢江辉,陈文娜.2018.香蕉野生种质资源的分类、分布和分子系统发育研究进展.园艺学报,45(9):1675-1687.
LüShun,Ren Yi,Wang Fang,Hu Gui-bing,Hung Bing-zhi,Liu Wen-qing,He Jian-qi,Liu Jian-ping,Zeng Li-sha,Zhou Jian-kun,Mai Jing-yu,Zhang Ke-heng.2018.Ploidy identification of 169 Musa germplasms by flow cytometry.Journal of Fruit Science,35(6):668-684.(in Chinese)吕顺,任毅,王芳,胡桂兵,黄秉智,刘文清,何建齐,刘建平,曾莉莎,周建坤,麦景郁,张珂恒.2018.利用流式细胞术快速鉴定169份香蕉种质资源的染色体倍性.果树学报,35(6):668-684.
Mabonga L,Pillay M.2017.SCAR marker for the a genome of bananas(Musa spp.L.)supports lack of differentiation between the A and B Genomes.Journal of Agricultural Science,9(6):64-73.
Manninen O,Kalendar R,Robinson J,Schulman A H.2000.Aplication of BARE-1 retrotransposon markers to map a major resistance gene for net blotch in barley.Molecular and General Genetics,264(3):325-334.
Nair A S,Teo C H,Schwarzacher T,Harrison P H.2005.Genome classification of banana cultivars from South India using IRAP markers.Euphytica,144:285-290.
Ning S P,Xu L B,Lu Y,Huang B Z,Ge X J.2007.Genome composition and genetic diversity of Musa germplasm from China revealed by PCR-RFLP and SSR markers.Scientia Horticulturae,114(4):281-288.
Nwakanma D C,Pillay M,Okoli B E,Tenkouano A.2003.PCR-RFLP of the ribosomal DNA internal transcribed spacers(ITS)provides markers for the A and B genomes in Musa L.Theoretical and Applied Genetics,108:154-159.
Onguso J M,Kahangi E M,Ndiritu D W,Mizutani F.2004.Genetic characterization of cultivated bananas and plantains in Kenya by RAPD markers.Scientia Horticulturae,99(1):9-20.
Pachuau L,Atom A D,Thangjam R.2014.Genome classification of Musa cultivars from Northeast India as revealed by ITS and IRAP markers.Appl Biochem Biotechnol,172:3939-3948.
Paran I,Michelmore R W.1993.Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce.Theor Appl Genet,85:985-993.
Pillay M,Nwakanma D C,Tenkouano A.2000.Identification of RAPD markers linked to A and B genome sequences in Musa L.Genome,43:763-767.
Pillay M,Ogundiwin E,Tenkouano A,Dolezel J.2006.Ploidyandgenome composition of Musa germplasm at the International Institute of Tropical Agriculture(IITA).Afr J Biotechnol,5:1224-1232.
Ramadass R,Sheriff M M.1993.Effect of moisture stress on the yield and yield components in banana as influenced by their genome and ploidy.Madras Agricultural Journal,80(3):130-133.
Robinson J C.1996.Bananas and plantains:crop production science in horticulture No.5.Wallingford,UK:CAB International:1-33.
Simmonds N W,Shepherd K.1955.The taxonomy and origins of the cultivated bananas.Botanical Journal of the Linnean Society,55:302-312.
Tang Dong-lan,Hu Yan,Gong Bang-chu,Zhang Qing-lin,Luo Zheng-rong.2016.Study on the hereditary variation of Jinzaoshi persimmon(Diospyros sp.)seedling progeny and the reproduction characteristics analyses based on SCoT and IRAP markers.Journal of Fruit Science,33(8):934-942.(in Chinese)唐冬兰,胡燕,龚榜初,张青林,罗正荣.2016.基于SCoT与IRAP标记的金枣柿实生后代遗传变异分析及其生殖特性.果树学报,33(8):934-942.
Teo C H,Tan S H,Ho C L,Faridah Q Z,Othman Y R,Heslop-Harrison J S,Kalendar R,Schulman A H.2005.Genome constitution and classification using retrotransposon-based markers in the orphan crop banana.Journal of Plant Biology,48(1):96-105.
Vakili N G.1967.The experimental formation of polyploidy and its effect in the genus Musa.Am J Bot,54:24-36.
Wang J Y,Zheng L S,Huang B Z,Liu W L,Wu Y T.2010.Development,characterization,and variability analysis of microsatellites from a commercial cultivar of Musa acuminata.Genetic Resources&Crop Evolution,57(4):553-563.
Wang Zhengxun,Lin Zhaoping,Pan Kunqing.1994.Cytogenetical studies in Musa(Eumusa).Acta Genetica Sinica,21(6):453-462.(in Chinese)王正询,林兆平,潘坤清.1994.蕉类的细胞遗传学研究.遗传学报,21(6):453-462.
Wei W,Fan Z Q,Chen J Y,Kuang J F,Lu W J,Shan W.2017.A banana PHD-type transcription factor MaPHD1 represses a cell wall-degradation gene Ma XTH6 during fruit ripening.Horticultural Plant Journal,3(5):190-198.
Wong C,Kiew R G,Set O,Lee S K,Gan Y Y.2002.Assessment of validity of the sections in Musa(Musaceae)using AFLP.Annals of Botany,90(2):231-238.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |