基于LC-TOF-MS分析不同品种和产地厚朴中化学成分的轮廓差异
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摘要
目的:对不同品种和不同产地厚朴的化学物质进行轮廓谱差异研究,筛选并鉴定影响该药材质量差异的特征性成分。方法:采用液相色谱-飞行时间-质谱联用技术(LC-TOF-MS)分析并获得不同品种和不同产地厚朴的色谱图数据集,运用变量统计分析方法比较不同品种和不同产地厚朴的化学轮廓差异,筛选出导致这些差异的特征性化学成分,并对其进行质谱分析和比对。结果:分析并鉴定出了11个特征峰,包括厚朴酚、和厚朴酚、石竹厚朴酚、厚朴碱、木兰花碱、鹅掌楸碱、罗默碱、海罂粟碱、阿西米洛宾、塔斯品碱、观音莲明碱。结论:LC-TOF-MS可简便、快速地对不同产地和不同品种厚朴中化学物质进行轮廓差异性研究,为后期厚朴品质评价和药效物质基础研究提供了一定的实验依据。
Objective: To study on the differences of profile spectra among chemical components in Magnoliae Officinalis Cortex from different varieties and habitats, and to screen and identify the characteristic components affecting the quality difference of this herb. Method: The chromatogram data sets of Magnoliae Officinalis Cortex from different varieties and habitats were obtained by liquid chromatography-time-of-flight-mass spectrometry( LC-TOF-MS). Principal component analysis,partial least squares-discriminant analysis and cluster analysis were used to compare the differences in chemical profiles among Magnoliae Officinalis Cortex from different varieties and habitats,and adopted to screen out the characteristic chemical constituents that resulted in these differences and to perform mass spectrometry analysis and comparison. Result: Eleven characteristic peaks were identified by LC-TOF-MS chromatographic data and reported in the literature. The use of chemical profile could distinguish different habitats of Magnoliae Officinalis Cortex,but could not completely distinguish different varieties of this herb. Conclusion: LC-TOF-MS can easily and quickly study on the profile differences of chemical substances in Magnoliae Officinalis Cortex from different varieties and different habitats,the results of this study can provide a theoretical basis for the quality evaluation and pharmacodynamic material basis of this herb.
Objective: To study on the differences of profile spectra among chemical components in Magnoliae Officinalis Cortex from different varieties and habitats, and to screen and identify the characteristic components affecting the quality difference of this herb. Method: The chromatogram data sets of Magnoliae Officinalis Cortex from different varieties and habitats were obtained by liquid chromatography-time-of-flight-mass spectrometry( LC-TOF-MS). Principal component analysis,partial least squares-discriminant analysis and cluster analysis were used to compare the differences in chemical profiles among Magnoliae Officinalis Cortex from different varieties and habitats,and adopted to screen out the characteristic chemical constituents that resulted in these differences and to perform mass spectrometry analysis and comparison. Result: Eleven characteristic peaks were identified by LC-TOF-MS chromatographic data and reported in the literature. The use of chemical profile could distinguish different habitats of Magnoliae Officinalis Cortex,but could not completely distinguish different varieties of this herb. Conclusion: LC-TOF-MS can easily and quickly study on the profile differences of chemical substances in Magnoliae Officinalis Cortex from different varieties and different habitats,the results of this study can provide a theoretical basis for the quality evaluation and pharmacodynamic material basis of this herb.
引文
[1]国家药典委员会.中华人民共和国药典.一部[M].北京:中国医药科技出版社,2015:251-252.
[2]翁德会,刘先琼,许腊英,等.不同等级厚朴饮片质量评价指标的系统聚类分析[J].中国实验方剂学杂志,2016,22(23):6-10.
[3]张春霞,杨立新,余星,等.种源、产地及采收树龄对厚朴药材质量的影响[J].中国中药杂志,2009,34(19):2431-2437.
[4]方小平,卢永书,吴琼,等.贵州省不同地区的凹叶厚朴挥发油成分GC-MS分析[J].中国实验方剂学杂志,2012,18(17):142-145.
[5]闫婕,卫莹芳,胡慧玲,等.全国不同产地厚朴药材品质评价[J].时珍国医国药,2016,27(2):472-474.
[6]董佳悦,刘美琳,任波.等.四川不同产地厚朴中挥发油及多糖含量分析[J].中药与临床,2017,8(2):22-25.
[7]胡慧玲,卫莹芳,马雪玮,等.不同加工方法对厚朴主要化学成分的影响研究[J].中成药,2011,33(5):834-837.
[8]薛珍珍,晏仁义,余盛贤,等. HPLC-DAD测定厚朴中6种活性成分的含量[J].中国实验方剂学杂志,2014,20(22):45-49.
[9]赵艳敏,刘素香,张晨曦,等.基于HPLC-Q-TOF-MS技术的甘草化学成分分析[J].中草药,2016,47(12):2061-2068.
[10]闫广利,孙晖,张爱华,等.基于中医方证代谢组学的中药质量标志物发现研究[J].中草药,2018,49(16):3729-3734.
[11] XIE G X,Plumb R,SU M M,et al. Ultra-performance LC/TOF MS analysis of medicinal Panax herbs for metabolomic research[J]. J Sep Sci,2008,31(6/7):1015-1026.
[12] KANG J H,Lee S Y,KANG S M,et al. NMR-based metabolomics approach for the differentiation of ginseng(Panax ginseng)roots from different origins[J]. Arch Pharm Res,2008,31(3):330-336.
[13] DAN M,SU M M,GAO X F,et al. Metabolite profiling of Panax notoginseng using UPLC-ESI-MS[J].Phytochemistry,2008,69(11):2237-2244.
[14] Toh D F,New L S,Koh H L,et al. Ultra-high performance liquid chromatography/time-of-flight mass spectrometry(UHPLC/TOFMS)for time-dependent profiling of raw and steamed Panax notoginseng[J]. J Pharm Biomed Anal,2010,52(1):43-50.
[15]张艳,王战国,兰轲.一种简便的用于中药质量评价的代谢组学方法[J/OL].中国科技论文在线,http://www. paper. edu. cn/index. php/default/releasepaper/content/201006-21.
[16]王安齐,王战国,杨俊毅,等.代谢组学技术在银杏叶片质量评价中的应用初探[[J/OL].中国科技论文在线,http://www. paper. edu. cn/index. php/default/releasepaper/content/201108-70.
[17] WANG Z G,HU H L,CHEN F,et al. Metabolic profiling assisted quality assessment of Rhodiola rosea extracts by high-performance liquid chromatography[J]. Planta Med,2012,78(7):740-746.
[18]高飞,傅超美,胡慧玲,等. HPLC-UV技术用于川木香煨制前后代谢物轮廓差异研究[J].中草药,2013,44(5):547-551.
[19]郭健.厚朴中生物碱成分及炮制地厚朴化学成分影响的研究[D].成都:西南交通大学,2012.
[20] HU H L,WANG Z G,HUA W,et al. Effect of chemical profiling change of processed Magnolia officinalis on the pharmacokinetic profiling of honokiol and magnolol in rats[J]. J Chromatogr Sci,2016,54(7):1201-1212.
[21] CHENG X,Harzdorf N,Khaing Z,et al. Neuronal growth promoting sesquiterpene-neolignans; syntheses and biological studies[J]. Org Biomol Chem,2012,10(2):383-393.
[22]余盛贤.基于水溶性成分分析的厚朴质量评价[D].北京:中国中医科学院,2011.
[23] WANG P,ZHU M Z,HUAGN W,et al. Lysicamine in a lotus leaves extract may be responsible for antibacterial and anti-inflammation activity[J]. AMM,2012,108:189-193.
[24] da Silva F M,de Souza A D,Koolen H H,et al.Phytochemical study of the alkaloidal fractions of Unonopsis duckei R. E. Fr. guided by electrospray ionisation ion-trap tandem mass spectrometry[J].Phytochem Anal,2014,25(1):45-49.
[25]钟圣海,付艳辉,周学明,等.瓜馥木枝叶中生物碱类化学成分研究[J].中国中药杂志,2016,41(15):2838-2842.
[26] Singh A,Bajpai V,Kumar S,et al. Analysis of isoquinoline alkaloids from Mahonia leschenaultia and Mahonia napaulensis roots using UHPLC-Orbitrap-MSn and UHPLC-QqQLIT-MS/MS[J]. J Pharm Anal,2017,7(2):77-86.
[27] De Marino S,Gala F,Zollo F,et al. Identification of minor secondary metabolites from the latex of Croton lechleri(Muell-Arg)and evaluation of their antioxidant activity[J]. Molecules,2008,13(6):1219-1229.
[28]赵慧,严颖,邹立思,等.基于LC-MS/MS和GC-MS/MS技术分析“川朴”与“温朴”的差异化学成分[J].天然产物研究与开发,2018,30(1):1-9.
[2]翁德会,刘先琼,许腊英,等.不同等级厚朴饮片质量评价指标的系统聚类分析[J].中国实验方剂学杂志,2016,22(23):6-10.
[3]张春霞,杨立新,余星,等.种源、产地及采收树龄对厚朴药材质量的影响[J].中国中药杂志,2009,34(19):2431-2437.
[4]方小平,卢永书,吴琼,等.贵州省不同地区的凹叶厚朴挥发油成分GC-MS分析[J].中国实验方剂学杂志,2012,18(17):142-145.
[5]闫婕,卫莹芳,胡慧玲,等.全国不同产地厚朴药材品质评价[J].时珍国医国药,2016,27(2):472-474.
[6]董佳悦,刘美琳,任波.等.四川不同产地厚朴中挥发油及多糖含量分析[J].中药与临床,2017,8(2):22-25.
[7]胡慧玲,卫莹芳,马雪玮,等.不同加工方法对厚朴主要化学成分的影响研究[J].中成药,2011,33(5):834-837.
[8]薛珍珍,晏仁义,余盛贤,等. HPLC-DAD测定厚朴中6种活性成分的含量[J].中国实验方剂学杂志,2014,20(22):45-49.
[9]赵艳敏,刘素香,张晨曦,等.基于HPLC-Q-TOF-MS技术的甘草化学成分分析[J].中草药,2016,47(12):2061-2068.
[10]闫广利,孙晖,张爱华,等.基于中医方证代谢组学的中药质量标志物发现研究[J].中草药,2018,49(16):3729-3734.
[11] XIE G X,Plumb R,SU M M,et al. Ultra-performance LC/TOF MS analysis of medicinal Panax herbs for metabolomic research[J]. J Sep Sci,2008,31(6/7):1015-1026.
[12] KANG J H,Lee S Y,KANG S M,et al. NMR-based metabolomics approach for the differentiation of ginseng(Panax ginseng)roots from different origins[J]. Arch Pharm Res,2008,31(3):330-336.
[13] DAN M,SU M M,GAO X F,et al. Metabolite profiling of Panax notoginseng using UPLC-ESI-MS[J].Phytochemistry,2008,69(11):2237-2244.
[14] Toh D F,New L S,Koh H L,et al. Ultra-high performance liquid chromatography/time-of-flight mass spectrometry(UHPLC/TOFMS)for time-dependent profiling of raw and steamed Panax notoginseng[J]. J Pharm Biomed Anal,2010,52(1):43-50.
[15]张艳,王战国,兰轲.一种简便的用于中药质量评价的代谢组学方法[J/OL].中国科技论文在线,http://www. paper. edu. cn/index. php/default/releasepaper/content/201006-21.
[16]王安齐,王战国,杨俊毅,等.代谢组学技术在银杏叶片质量评价中的应用初探[[J/OL].中国科技论文在线,http://www. paper. edu. cn/index. php/default/releasepaper/content/201108-70.
[17] WANG Z G,HU H L,CHEN F,et al. Metabolic profiling assisted quality assessment of Rhodiola rosea extracts by high-performance liquid chromatography[J]. Planta Med,2012,78(7):740-746.
[18]高飞,傅超美,胡慧玲,等. HPLC-UV技术用于川木香煨制前后代谢物轮廓差异研究[J].中草药,2013,44(5):547-551.
[19]郭健.厚朴中生物碱成分及炮制地厚朴化学成分影响的研究[D].成都:西南交通大学,2012.
[20] HU H L,WANG Z G,HUA W,et al. Effect of chemical profiling change of processed Magnolia officinalis on the pharmacokinetic profiling of honokiol and magnolol in rats[J]. J Chromatogr Sci,2016,54(7):1201-1212.
[21] CHENG X,Harzdorf N,Khaing Z,et al. Neuronal growth promoting sesquiterpene-neolignans; syntheses and biological studies[J]. Org Biomol Chem,2012,10(2):383-393.
[22]余盛贤.基于水溶性成分分析的厚朴质量评价[D].北京:中国中医科学院,2011.
[23] WANG P,ZHU M Z,HUAGN W,et al. Lysicamine in a lotus leaves extract may be responsible for antibacterial and anti-inflammation activity[J]. AMM,2012,108:189-193.
[24] da Silva F M,de Souza A D,Koolen H H,et al.Phytochemical study of the alkaloidal fractions of Unonopsis duckei R. E. Fr. guided by electrospray ionisation ion-trap tandem mass spectrometry[J].Phytochem Anal,2014,25(1):45-49.
[25]钟圣海,付艳辉,周学明,等.瓜馥木枝叶中生物碱类化学成分研究[J].中国中药杂志,2016,41(15):2838-2842.
[26] Singh A,Bajpai V,Kumar S,et al. Analysis of isoquinoline alkaloids from Mahonia leschenaultia and Mahonia napaulensis roots using UHPLC-Orbitrap-MSn and UHPLC-QqQLIT-MS/MS[J]. J Pharm Anal,2017,7(2):77-86.
[27] De Marino S,Gala F,Zollo F,et al. Identification of minor secondary metabolites from the latex of Croton lechleri(Muell-Arg)and evaluation of their antioxidant activity[J]. Molecules,2008,13(6):1219-1229.
[28]赵慧,严颖,邹立思,等.基于LC-MS/MS和GC-MS/MS技术分析“川朴”与“温朴”的差异化学成分[J].天然产物研究与开发,2018,30(1):1-9.
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