血清微小RNA-21水平与糖尿病肾病患者氧化应激的相关性研究
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摘要
目的:探讨血清微小RNA-21(mi R-21)水平与糖尿病肾病(DN)患者氧化应激的相关性。方法:选择2015年1月到2017年5月我院就诊的169例DN患者作为研究对象,其中DN分期早期组95例,中晚期组74例。另选同期在我院接受健康体检者90例作为对照组,对比各组血清mi R-21水平和氧化应激指标,分析患者血清mi R-21水平与氧化应激指标的相关性。结果:各组血清mi R-21水平相比差异有统计学意义(P<0.05),中晚期DN组及早期DN组血清mi R-21水平均明显低于对照组,且中晚期DN组又明显低于早期DN组,差异均有统计学意义(P<0.05)。各组氧化应激指标相比差异有统计学意义(P<0.05),中晚期DN组及早期DN组丙二醛(MDA)、超氧化物歧化酶(SOD)、晚期氧化蛋白产物(AOPP)及NADPH氧化酶4(NOX4)均明显高于对照组,且中晚期DN组又明显高于早期DN组;而血红素氧合酶1(HO-1)明显低于对照组,且中晚期DN组又明显低于早期DN组,差异均有统计学意义(P<0.05)。依据Spearman法分析相关性发现,血清miR-21水平与MDA、SOD、AOPP及NOX4均呈负相关(P<0.05),与HO-1呈正相关(P<0.05)。结论:血清mi R-21水平与DN患者氧化应激指标均具有明显的相关性,临床上可尝试将miR-21纳入到DN患者病情监测的指标体系中,从而有助于疾病的诊治及患者的预后。
Objective: To investigate the correlation between serum microRNA-21(mi R-21) level and oxidative stress in patients with diabetic nephropathy(DN). Methods: 169 cases of DN patients in our hospital from January 2015 to May 2017 were selected as the study subjects, including 95 cases in early DN group and 74 cases in middle-late DN group. In addition, 90 healthy people who received physical examination in our hospital during the same period were selected as control group. The levels of serum mi R-21 and oxidative stress indicators were compared in each group, and the correlation between the levels of serum mi R-21 and oxidative stress indicators was analyzed. Results: The levels of serum mi R-21 in each group had significant difference(P<0.05). The levels of serum mi R-21 in middle-late DN group and early DN group were significantly lower than those in control group, and the levels in middle-late DN group were significantly lower than those in early DN group, the difference was statistically significant(P<0.05). There were significant differences in oxidative stress indicators among the groups(P<0.05), malondialdehyde(MDA), superoxide dismutase(SOD), advanced oxidative protein product(AOPP) and NADPH Oxidase 4(NOX4) in the middle-late DN group and early DN group were significantly higher than those in the control group, and those in the middle-late DN group were significantly higher than those in the early DN group, while heme oxygenase-1(HO-1) was significantly lower than that of the control group, and in the middle-late DN group was significantly lower than that of the early DN group, the difference was statistically significant(P<0.05). According to Spearman method, serum levels of mi R-21 were negatively correlated with MDA, SOD, AOPP and NOX4(P<0.05), and positively correlated with HO-1(P<0.05). Conclusion:Serum level of mi R-21 is significantly correlated with oxidative stress indicators in DN patients. In clinical practice, mi R-21 can be incorporated into the indicator system of DN patients' condition monitoring, thus contributing to the diagnosis and treatment of diseases and the prognosis of patients.
Objective: To investigate the correlation between serum microRNA-21(mi R-21) level and oxidative stress in patients with diabetic nephropathy(DN). Methods: 169 cases of DN patients in our hospital from January 2015 to May 2017 were selected as the study subjects, including 95 cases in early DN group and 74 cases in middle-late DN group. In addition, 90 healthy people who received physical examination in our hospital during the same period were selected as control group. The levels of serum mi R-21 and oxidative stress indicators were compared in each group, and the correlation between the levels of serum mi R-21 and oxidative stress indicators was analyzed. Results: The levels of serum mi R-21 in each group had significant difference(P<0.05). The levels of serum mi R-21 in middle-late DN group and early DN group were significantly lower than those in control group, and the levels in middle-late DN group were significantly lower than those in early DN group, the difference was statistically significant(P<0.05). There were significant differences in oxidative stress indicators among the groups(P<0.05), malondialdehyde(MDA), superoxide dismutase(SOD), advanced oxidative protein product(AOPP) and NADPH Oxidase 4(NOX4) in the middle-late DN group and early DN group were significantly higher than those in the control group, and those in the middle-late DN group were significantly higher than those in the early DN group, while heme oxygenase-1(HO-1) was significantly lower than that of the control group, and in the middle-late DN group was significantly lower than that of the early DN group, the difference was statistically significant(P<0.05). According to Spearman method, serum levels of mi R-21 were negatively correlated with MDA, SOD, AOPP and NOX4(P<0.05), and positively correlated with HO-1(P<0.05). Conclusion:Serum level of mi R-21 is significantly correlated with oxidative stress indicators in DN patients. In clinical practice, mi R-21 can be incorporated into the indicator system of DN patients' condition monitoring, thus contributing to the diagnosis and treatment of diseases and the prognosis of patients.
引文
[1]冯超杰,陈争跃,包蓓艳.糖尿病肾病标志物的研究进展[J].生理科学进展,2018,49(5):353-358
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[3]陈松,顾波,王汉清,等.硝苯地平控释片联合缬沙坦治疗对2型糖尿病肾病合并高血压患者血清学指标的影响[J].海南医学院学报2016,22(13):1398-1401
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[6]阮芸,姚佳琦,张珍瑛,等.糖尿病肾病患者血清Leptin、Vaspin含量与肾功能、氧化应激及炎症反应的相关性[J].海南医学院学报,2018,24(17):1562-1566
[7]李奕茜,马建,王洋,等.NLRP3炎性体在糖尿病肾病炎症机制中作用[J].辽宁中医药大学学报,2018,20(1):126-129
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[9]Wang G,Yan Y,Xu N,et al.Upregulation of micro RNA-424 relieved diabetic nephropathy by targeting Rictor through m TOR Complex2/Protein Kinase B signaling[J].J Cell Physiol,2019,234(7):11646-11653
[10]Xourgia E,Papazafiropoulou A,Melidonis A.Circulating micro R-NAs as biomarkers for diabetic neuropathy:A novel approach[J].World J Exp Med,2018,8(3):18-23
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[12]中华医学会糖尿病学分会.中国2型糖尿病防治指南(2013年版)[J].中华内分泌代谢杂志,2014,30(10):893-942
[13]李云程,应长江,李伟.2型糖尿病合并血管并发症患者纤维蛋白原水平的变化及其与血糖波动的相关性研究[J].中国糖尿病杂志,2018,26(10):830-834
[14]赵珊,张瑞霞.NLR与2型糖尿病及其慢性并发症研究进展[J].现代临床医学,2017,43(4):309-312
[15]Jin J,Shi Y,Gong J,et al.Exosome secreted from adipose-derived stem cells attenuates diabetic nephropathy by promoting autophagy flux and inhibiting apoptosis in podocyte[J].Stem Cell Res Ther,2019,10(1):95
[16]Yang M,Kan L,Wu L,et al.Effect of baicalin on renal function in patients with diabetic nephropathy and its therapeutic mechanism[J].Exp Ther Med,2019,17(3):2071-2076
[17]叶华红,游晓蓉.糖尿病肾病患者血管功能及循环血mi R指标的变化研究[J].临床和实验医学杂志,2016,15(12):1178-1180
[18]赵艾苹,魏剑芬,王建荣,等.糖尿病微血管病变患者血清25-OH维生素D3水平的变化及其影响因素[J].中国医师杂志,2019,21(1):102-104
[19]Shang J,Wang S,Jiang Y,et al.Identification of key lnc RNAs contributing to diabetic nephropathy by gene co-expression network analysis[J].Sci Rep,2019,9(1):3328
[20]陈桐,范秋灵,崔靖彬,等.糖尿病和糖尿病肾病患者血清lnc RNAGAS5/mi R-21的诊断效能[J].中华肾脏病杂志,2017,33(12):906-913
[21]王金华,王俭勤,梁耀军,等.micro RNAs与糖尿病肾病[J].生理科学进展,2015,46(3):225-229
[22]赵雯雯,涂晓文.Mi R-21及其信号通路在糖尿病肾病中的研究进展[J].中国中西医结合肾病杂志,2014,15(8):747-749
[23]Gholaminejad A,Abdul Tehrani H,Gholami Fesharaki M.Identification of candidate micro RNA biomarkers in diabetic nephropathy:a meta-analysis of profiling studies[J].J Nephrol,2018,31(6):813-831
[24]左满花,唐俊.不同分期老年糖尿病肾病患者血清氧化应激、血管新生指标以及外周血T细胞含量的评估[J].中国现代医学杂志,2015,25(31):74-77
[25]张伟开,张玲.糖尿病肾病患者SOD-Mn基因多态性与肾功能、氧化性损伤的相关性[J].海南医学院学报,2018,24(14):1311-1314,1318
[26]饶毅峰,杨林,杜京涛,等.糖尿病肾病患者尿白蛋白排泄率与肾功能、炎症反应、氧化应激反应的相关性[J].海南医学院学报,2017,23(6):769-772
[27]何建秋,王艳新,孙志新,等.糖尿病肾病患者血尿酸与氧化应激的相关性分析[J].医学临床研究,2018,35(1):106-108
[28]努尔斯曼古丽·奥斯曼,焦谊,梁小弟,等.维吾尔族糖尿病肾病患者血清微小RNA的表达及其与糖尿病肾病的相关性[J].中华糖尿病杂志,2017,9(4):231-236
[29]冯罡,李军,王晓丽,等.糖尿病肾病患者血清micro RNA-21含量及其与氧化应激指标的相关性[J].中国现代医学杂志,2017,27(1):60-63
[30]Bijkerk R,Duijs JM,Kuairoun M,et al.Circulating micro RNAs asscoiate with diabetic nephropathy and systemic microvascular samage and normalize after simultaneous pancreas-kidney thansplantation[J].Am J Transplant,2015,15(4):1081-1090
[2]沈义兰,丁涛,梅小斌.糖尿病肾病的生物治疗及研究进展[J].药学服务与研究,2018,18(6):401-405
[3]陈松,顾波,王汉清,等.硝苯地平控释片联合缬沙坦治疗对2型糖尿病肾病合并高血压患者血清学指标的影响[J].海南医学院学报2016,22(13):1398-1401
[4]Naing C,Htet NH,Basavaraj AK,et al.An association between IL-10promoter polymorphisms and diabetic nephropathy:a meta-analysis of case-control studies[J].J Diabetes Metab Disord,2018,17(2):333-343
[5]Konari N,Nagaishi K,Kikuchi S,et al.Mitochondria transfer from mesenchymal stem cells structurally and functionally repairs renal proximal tubular epithelial cells in diabetic nephropathy in vivo[J].Sci Rep,2019,9(1):5184
[6]阮芸,姚佳琦,张珍瑛,等.糖尿病肾病患者血清Leptin、Vaspin含量与肾功能、氧化应激及炎症反应的相关性[J].海南医学院学报,2018,24(17):1562-1566
[7]李奕茜,马建,王洋,等.NLRP3炎性体在糖尿病肾病炎症机制中作用[J].辽宁中医药大学学报,2018,20(1):126-129
[8]王彦珺,涂晓文.mi RNA在糖尿病肾病中的临床应用进展[J].中国中西医结合肾病杂志,2014,15(2):180-181
[9]Wang G,Yan Y,Xu N,et al.Upregulation of micro RNA-424 relieved diabetic nephropathy by targeting Rictor through m TOR Complex2/Protein Kinase B signaling[J].J Cell Physiol,2019,234(7):11646-11653
[10]Xourgia E,Papazafiropoulou A,Melidonis A.Circulating micro R-NAs as biomarkers for diabetic neuropathy:A novel approach[J].World J Exp Med,2018,8(3):18-23
[11]刘玲伶,刘丽荣,王圆圆,等.糖尿病大鼠肾组织中mi R-21和Sno N表达的变化[J].基础医学与临床,2017,37(1):32-37
[12]中华医学会糖尿病学分会.中国2型糖尿病防治指南(2013年版)[J].中华内分泌代谢杂志,2014,30(10):893-942
[13]李云程,应长江,李伟.2型糖尿病合并血管并发症患者纤维蛋白原水平的变化及其与血糖波动的相关性研究[J].中国糖尿病杂志,2018,26(10):830-834
[14]赵珊,张瑞霞.NLR与2型糖尿病及其慢性并发症研究进展[J].现代临床医学,2017,43(4):309-312
[15]Jin J,Shi Y,Gong J,et al.Exosome secreted from adipose-derived stem cells attenuates diabetic nephropathy by promoting autophagy flux and inhibiting apoptosis in podocyte[J].Stem Cell Res Ther,2019,10(1):95
[16]Yang M,Kan L,Wu L,et al.Effect of baicalin on renal function in patients with diabetic nephropathy and its therapeutic mechanism[J].Exp Ther Med,2019,17(3):2071-2076
[17]叶华红,游晓蓉.糖尿病肾病患者血管功能及循环血mi R指标的变化研究[J].临床和实验医学杂志,2016,15(12):1178-1180
[18]赵艾苹,魏剑芬,王建荣,等.糖尿病微血管病变患者血清25-OH维生素D3水平的变化及其影响因素[J].中国医师杂志,2019,21(1):102-104
[19]Shang J,Wang S,Jiang Y,et al.Identification of key lnc RNAs contributing to diabetic nephropathy by gene co-expression network analysis[J].Sci Rep,2019,9(1):3328
[20]陈桐,范秋灵,崔靖彬,等.糖尿病和糖尿病肾病患者血清lnc RNAGAS5/mi R-21的诊断效能[J].中华肾脏病杂志,2017,33(12):906-913
[21]王金华,王俭勤,梁耀军,等.micro RNAs与糖尿病肾病[J].生理科学进展,2015,46(3):225-229
[22]赵雯雯,涂晓文.Mi R-21及其信号通路在糖尿病肾病中的研究进展[J].中国中西医结合肾病杂志,2014,15(8):747-749
[23]Gholaminejad A,Abdul Tehrani H,Gholami Fesharaki M.Identification of candidate micro RNA biomarkers in diabetic nephropathy:a meta-analysis of profiling studies[J].J Nephrol,2018,31(6):813-831
[24]左满花,唐俊.不同分期老年糖尿病肾病患者血清氧化应激、血管新生指标以及外周血T细胞含量的评估[J].中国现代医学杂志,2015,25(31):74-77
[25]张伟开,张玲.糖尿病肾病患者SOD-Mn基因多态性与肾功能、氧化性损伤的相关性[J].海南医学院学报,2018,24(14):1311-1314,1318
[26]饶毅峰,杨林,杜京涛,等.糖尿病肾病患者尿白蛋白排泄率与肾功能、炎症反应、氧化应激反应的相关性[J].海南医学院学报,2017,23(6):769-772
[27]何建秋,王艳新,孙志新,等.糖尿病肾病患者血尿酸与氧化应激的相关性分析[J].医学临床研究,2018,35(1):106-108
[28]努尔斯曼古丽·奥斯曼,焦谊,梁小弟,等.维吾尔族糖尿病肾病患者血清微小RNA的表达及其与糖尿病肾病的相关性[J].中华糖尿病杂志,2017,9(4):231-236
[29]冯罡,李军,王晓丽,等.糖尿病肾病患者血清micro RNA-21含量及其与氧化应激指标的相关性[J].中国现代医学杂志,2017,27(1):60-63
[30]Bijkerk R,Duijs JM,Kuairoun M,et al.Circulating micro RNAs asscoiate with diabetic nephropathy and systemic microvascular samage and normalize after simultaneous pancreas-kidney thansplantation[J].Am J Transplant,2015,15(4):1081-1090