黑龙江省主栽水稻品种抗稻瘟病基因的分子检测与分析
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摘要
了解品种本身的抗性基因型对于水稻品种合理布局具有重要意义。为了明确稻瘟病抗性基因Pi-ta、Pi-b、Pik-m、Pi9、Pii、Pi-d3在黑龙江省水稻品种中的分布情况,选取34份主栽品种,利用这6个抗瘟基因的功能标记,对供试材料进行分子标记检测。结果表明,Pi9的分布频率最高,其次是Pi-ta和Pik-m,Pi-b和Pii抗性基因分布频率较低,Pi-d3的分布频率最低;供试的34份水稻材料中,被检测出最多含有5个抗性基因,而最少只含有1个抗性基因,含有2个抗性基因的品种所占比例最大,龙粳40和龙粳42不含有待检测基因。
Understanding the resistance genotype of the variety itself is great significant for the rational layout of rice varieties.In order to clarify the distribution of rice blast resistance genes Pi-ta,Pi-b,Pik-m,Pi9,Pii and Pi-d3 in rice varieties in Heilongjiang Province,34 main varieties were selected,and the functional markers of these 6 anti-blast genes were used to detect the molecular markers.Results showed that the distribution frequency of Pi9 was the highest,followed by Pi-ta and Pik-m,the frequency of Pi-b and Pii resistance genes was lower,and the distribution frequency of Pi-d3 was the lowest.Among the 34 rice materials tested,some varieties contained up to five resistance genes and some contained at least one resistance gene.The species with two resistance genes accounted for the largest proportion.Longjing 40 and Longjing 42 did not contain the gene to be detected.
Understanding the resistance genotype of the variety itself is great significant for the rational layout of rice varieties.In order to clarify the distribution of rice blast resistance genes Pi-ta,Pi-b,Pik-m,Pi9,Pii and Pi-d3 in rice varieties in Heilongjiang Province,34 main varieties were selected,and the functional markers of these 6 anti-blast genes were used to detect the molecular markers.Results showed that the distribution frequency of Pi9 was the highest,followed by Pi-ta and Pik-m,the frequency of Pi-b and Pii resistance genes was lower,and the distribution frequency of Pi-d3 was the lowest.Among the 34 rice materials tested,some varieties contained up to five resistance genes and some contained at least one resistance gene.The species with two resistance genes accounted for the largest proportion.Longjing 40 and Longjing 42 did not contain the gene to be detected.
引文
[1]罗曼斯.水稻抗稻瘟病候选基因的筛选及功能验证.南昌:南昌大学,2017.
[2]汤智鹏.59份水稻种质资源对稻瘟病的抗性及农艺性状评价.雅安:四川农业大学,2011.
[3]李恩宇,王悦,陈光辉.水稻稻瘟病抗性基因的定位及克隆研究进展.作物研究,2014,28(6):754-760.
[4]蔡金洋.分子标记在水稻抗稻瘟病育种中的研究进展.农业科技通讯,2018(6):4-5,9.
[5]Bryan G T,Wu K S,Farrall L,et al.tA single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pi-ta.The Plant Cell,2000,12(11):2033-2045.
[6]Wang Z X,Yano M,Yamanouchi U,et al.The Pib gene for blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal,1999,19(1):55-64.
[7]刘洋,徐培洲,张红宇,等.水稻抗稻瘟病Pib基因的分子标记辅助选择与应用.中国农业科学,2008,41(1):9-14.
[8]张羽.区域水稻品种的Pi-km基因的分析.陕西理工学院学报(自然科学版),2013,29(4):61-65.
[9]Stefano C,Yulin J.Sequence variation at the rice blast resistance gene Pi-km locus:Implications for the development of allele specific markers.Plant Science,2010,178(6):523-530.
[10]Li L Y,Wang L,Jing J X,et al.The Pikm gene,conferring stable resistance to isolates of Magnaporthe oryzae,was finely mapped in a crossover-cold region on rice chromosome 11.Molecular Breeding,2007,20(2):179-188.
[11]Qu S H,Liu G F,Zhou B.et al.The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice.Genetics,2006,172(3):1901-1914.
[12]Liu G,Lu G,Zeng L,et al.Two broad-spectrum blast resistance genes,Pi9(t)and Pi2(t),are physically linked on rice chromosome 6.Molecular Genetics and Genomics,2002,267(4):472-480.
[13]周鹏,涂诗航,董瑞霞,等.水稻抗稻瘟病基因分子检测及抗性评价.福建农业学报,2016,31(9):962-965.
[14]朱立宏.主要农作物抗病性遗传研究进展.南京:江苏科学技术出版社,1990:83-93.
[15]李志.9个抗稻瘟病基因自然抗性评价及育种应用研究.武汉:华中农业大学,2017.
[16]兰波,杨迎青,陈建,等.江西水稻主栽品种的稻瘟病抗性基因分子标记检测与分析.分子植物育种,2019,17(8):2559-2567.
[17]Wang Z,Jia Y,Rutger J N,et al.Rapid survey for presence of a blast resistance gene Pi-ta in rice cultivars using the dominant DNAmarkers derived from portions of the Pi-ta gene.Plant Breeding,2010,126(1):36-42.
[18]Robert F,Concetta A C B,Anna M,et al.Development of DNAmarkers suitable for marker assisted selection of three,genes conferring resistance to multiple,pathotypes.Crop Science,2004,44(5):1790-1798.
[19]Ashikawa I,Hayashi N,Yamane H,et al.Two adjacent nucleotidebinding site-leucine-rich repeat class genes are required to confer Pikm-specific rice blast resistance.Genetics,2008,180(4):2267-2276.
[20]张羽,冯志峰,张晗,等.陕西省水稻种质资源中Pi9基因的分布状况.四川农业大学学报,2013,31(2):115-121.
[21]Takagi H,Uemura A,Yaegashi H,et al.MutMap-Gap:wholegenome resequencing of mutant F2 progeny bulk combined with de novo assembly of gap regions identifies the rice blast resistance gene Pii,New Phytologist,2013,200(1):276-283.
[22]王亚,陈献功,尹海庆,等.河南主要水稻种质资源中抗稻瘟病基因的分子检测.分子植物育种,2018,16(10):3203-3212.
[23]时克,雷财林,程治军,等.稻瘟病抗性基因Pita和Pib在我国水稻主栽品种中的分布.植物遗传资源学报,2009,10(1):21-26.
[24]陈峰,徐建第,姜明松,等.黄淮区粳稻抗稻瘟病基因Pi-ta、Pi-b、Pi54、Pikm的分子检测.生物技术进展,2018,8(1):46-54.
[25]张银霞,张敏,田蕾,等.宁夏水稻品种抗稻瘟病基因Pi-ta、Pi-b和Pi9的检测分析.江苏农业科学,2016,44(9):35-39.
[26]王军,杨杰,杨金欢,等.Pi-ta、Pi-b基因在江苏粳稻穗颈瘟抗性育种中的价值分析.华北农学报,2012,27(6):141-145.
[27]于连鹏,张亚玲,刘殿宇,等.黑龙江省主栽水稻品种对稻瘟病的抗性评价与利用.江苏农业科学,2018,46(9):115-118.
[2]汤智鹏.59份水稻种质资源对稻瘟病的抗性及农艺性状评价.雅安:四川农业大学,2011.
[3]李恩宇,王悦,陈光辉.水稻稻瘟病抗性基因的定位及克隆研究进展.作物研究,2014,28(6):754-760.
[4]蔡金洋.分子标记在水稻抗稻瘟病育种中的研究进展.农业科技通讯,2018(6):4-5,9.
[5]Bryan G T,Wu K S,Farrall L,et al.tA single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pi-ta.The Plant Cell,2000,12(11):2033-2045.
[6]Wang Z X,Yano M,Yamanouchi U,et al.The Pib gene for blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes.The Plant Journal,1999,19(1):55-64.
[7]刘洋,徐培洲,张红宇,等.水稻抗稻瘟病Pib基因的分子标记辅助选择与应用.中国农业科学,2008,41(1):9-14.
[8]张羽.区域水稻品种的Pi-km基因的分析.陕西理工学院学报(自然科学版),2013,29(4):61-65.
[9]Stefano C,Yulin J.Sequence variation at the rice blast resistance gene Pi-km locus:Implications for the development of allele specific markers.Plant Science,2010,178(6):523-530.
[10]Li L Y,Wang L,Jing J X,et al.The Pikm gene,conferring stable resistance to isolates of Magnaporthe oryzae,was finely mapped in a crossover-cold region on rice chromosome 11.Molecular Breeding,2007,20(2):179-188.
[11]Qu S H,Liu G F,Zhou B.et al.The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice.Genetics,2006,172(3):1901-1914.
[12]Liu G,Lu G,Zeng L,et al.Two broad-spectrum blast resistance genes,Pi9(t)and Pi2(t),are physically linked on rice chromosome 6.Molecular Genetics and Genomics,2002,267(4):472-480.
[13]周鹏,涂诗航,董瑞霞,等.水稻抗稻瘟病基因分子检测及抗性评价.福建农业学报,2016,31(9):962-965.
[14]朱立宏.主要农作物抗病性遗传研究进展.南京:江苏科学技术出版社,1990:83-93.
[15]李志.9个抗稻瘟病基因自然抗性评价及育种应用研究.武汉:华中农业大学,2017.
[16]兰波,杨迎青,陈建,等.江西水稻主栽品种的稻瘟病抗性基因分子标记检测与分析.分子植物育种,2019,17(8):2559-2567.
[17]Wang Z,Jia Y,Rutger J N,et al.Rapid survey for presence of a blast resistance gene Pi-ta in rice cultivars using the dominant DNAmarkers derived from portions of the Pi-ta gene.Plant Breeding,2010,126(1):36-42.
[18]Robert F,Concetta A C B,Anna M,et al.Development of DNAmarkers suitable for marker assisted selection of three,genes conferring resistance to multiple,pathotypes.Crop Science,2004,44(5):1790-1798.
[19]Ashikawa I,Hayashi N,Yamane H,et al.Two adjacent nucleotidebinding site-leucine-rich repeat class genes are required to confer Pikm-specific rice blast resistance.Genetics,2008,180(4):2267-2276.
[20]张羽,冯志峰,张晗,等.陕西省水稻种质资源中Pi9基因的分布状况.四川农业大学学报,2013,31(2):115-121.
[21]Takagi H,Uemura A,Yaegashi H,et al.MutMap-Gap:wholegenome resequencing of mutant F2 progeny bulk combined with de novo assembly of gap regions identifies the rice blast resistance gene Pii,New Phytologist,2013,200(1):276-283.
[22]王亚,陈献功,尹海庆,等.河南主要水稻种质资源中抗稻瘟病基因的分子检测.分子植物育种,2018,16(10):3203-3212.
[23]时克,雷财林,程治军,等.稻瘟病抗性基因Pita和Pib在我国水稻主栽品种中的分布.植物遗传资源学报,2009,10(1):21-26.
[24]陈峰,徐建第,姜明松,等.黄淮区粳稻抗稻瘟病基因Pi-ta、Pi-b、Pi54、Pikm的分子检测.生物技术进展,2018,8(1):46-54.
[25]张银霞,张敏,田蕾,等.宁夏水稻品种抗稻瘟病基因Pi-ta、Pi-b和Pi9的检测分析.江苏农业科学,2016,44(9):35-39.
[26]王军,杨杰,杨金欢,等.Pi-ta、Pi-b基因在江苏粳稻穗颈瘟抗性育种中的价值分析.华北农学报,2012,27(6):141-145.
[27]于连鹏,张亚玲,刘殿宇,等.黑龙江省主栽水稻品种对稻瘟病的抗性评价与利用.江苏农业科学,2018,46(9):115-118.