促进转录因子Nrf2表达的中药文献分析——体内抗氧化药物筛选
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摘要
目的:探讨刺激转录因子Nrf2信号通路,调节肌体抗氧化、促还原的药食中药,确定药名。方法:采用中国期刊全文专题数据库(CNKI)等以关键词"转录因子Nrf2""中药筛选""文献分析"进行检索,按照纳入和排除标准筛选2004-2014年公开发表的文献,用Excel2007创建文献题录库,存储、提取数据;建立文献计量统计分析与多指标评价相结合的方法:抗氧化,影响Ⅱ相解毒酶与蛋白激酶(GST、UGT、NQO1、GSH、ERK、JNK)活性,刺激Nrf2诱导Ⅱ相解毒酶表达,比较中药刺激Nrf2的强弱。结果:纳入符合要求的文献共计57篇,包括中药28种,化合物40种,疾病16种。结论:筛选出22种与指标评价相符的药物,其中刺激Nrf2诱导Ⅱ相解毒酶表达最强的中药为姜黄、洋甘菊、红车轴草。
Objective: To explore the signal pathway of stimulate transcription factor Nrf2, regulating the body antioxidant,promoting the reduction of medicinal and edible Chinese herbal medicine, and making sure the drug name. Methods: "tran-scription factor Nrf2", "Chinese medicine screen" and "literature analysis" were used as keywords to retrieve by using China journal full-text database(CNKI), et al. According to include and exclude standards, the published literatures were selected during 2004-2014. To create literature documents, storage and extract the data using Excel 2007, and establish method through qualitative analysis combined with multi-index evaluations: oxidation,influence Ⅱ detoxifying enzymes and protein kinase(GST, UGT, NQO1, GSH, ERK and JNK) activity,stimulate Nrf2 induction Ⅱ detoxifying enzyme expression, and compare the strength of the traditional Chinese medicine to stimulate the Nrf2. Results: According to the requirement references,57 literatures were included, including 28 kinds of Chinese medicine, 40 kinds of compounds, and 16 kinds of disease. Con-clusion: There were 22 kinds of drugs screened,which were matching the index evaluation, and the strongest expression of tra-ditional Chinese medicine for the stimulus Nrf2 induction Ⅱ phase detoxifying enzyme are turmeric, chamomile and trifolium pratense.
Objective: To explore the signal pathway of stimulate transcription factor Nrf2, regulating the body antioxidant,promoting the reduction of medicinal and edible Chinese herbal medicine, and making sure the drug name. Methods: "tran-scription factor Nrf2", "Chinese medicine screen" and "literature analysis" were used as keywords to retrieve by using China journal full-text database(CNKI), et al. According to include and exclude standards, the published literatures were selected during 2004-2014. To create literature documents, storage and extract the data using Excel 2007, and establish method through qualitative analysis combined with multi-index evaluations: oxidation,influence Ⅱ detoxifying enzymes and protein kinase(GST, UGT, NQO1, GSH, ERK and JNK) activity,stimulate Nrf2 induction Ⅱ detoxifying enzyme expression, and compare the strength of the traditional Chinese medicine to stimulate the Nrf2. Results: According to the requirement references,57 literatures were included, including 28 kinds of Chinese medicine, 40 kinds of compounds, and 16 kinds of disease. Con-clusion: There were 22 kinds of drugs screened,which were matching the index evaluation, and the strongest expression of tra-ditional Chinese medicine for the stimulus Nrf2 induction Ⅱ phase detoxifying enzyme are turmeric, chamomile and trifolium pratense.
引文
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[19]侯成成,麻微微,肖荣.等.染料木黄铜对星形胶质细胞氧化损伤中Nrf2-ARE通路相关因子表达的调节作用[J].中国食品卫生杂志,2013,25(1):1-5.
[20]王素琴,汤玲君,王艳.芦丁对糖尿病肾病大鼠的保护作用及其机制探讨[J].医学研究杂志,2012,41(10):144-148.
[21]元海丹,李海燕,王瑜玲,等.芦丁对Hep G2肝癌细胞AMPK蛋白信号通路的激活作用[J].延边大学医学学报,2013,36(3):180-182.
[2]南京中医药大学.中药大辞典(上、下册)(M).南京:南京大学出版社,2006:3.
[3]梅全喜.现代中药药理与临床应用手册[M].北京:中国中医药出版社出版,2008:10.
[4]潘晓菲,谭初兵,时丽丽.等.Nrf2/ARE信号通路及其相关药物研究进展[J].药物评价研究,2013,36(1):54-58.
[5]叶社房,侯振清,钟李明.等.姜黄素对Ⅱ相解GST NQO酶活性的诱导及其机制[J].药学学报,2007,42(4):376-380.
[6]袁俊华,李延青,杨晓云.Nrf2在EGCG诱导结肠肿瘤组织UGT1A及其同工酶表达中的作用[J].基础医学与临床,2010,30(12):1257-1262.
[7]韩永龙,李丹,孙习鹏,等.中药对Ⅱ相药物代谢酶GST和UGT的影响[J].中国药房,2010,21(3):274-276.
[8]倪静,秦素娟,吴拥军,等.肺癌组织中Nrf2、Keap1和NQO1蛋白的表达[J].郑州大学学报:医学版,2012,47(4):456-459.
[9]李强,左曙光,王旭霞.等.姜黄素激活转录因子Nrf2对人肝细胞氧化应激的影响[J].胃肠病学和肝病学杂志,2010,19(2):154-156.
[10]卢永科,川岛明,崛井郁夫.等.还原型谷胱甘肽对不同药物所致肾细胞毒性的影响[J].毒理学杂志,2005,19(1):45-46.
[11]关欣,郑红光,徐爱华.胞外调节蛋白激酶在糖尿病肾病中激活机制及健脾益肾活血中药的干预作用[J].云南中医学院学报,2007,30(6):24-27.
[12]刘頔,张孟仁.JNK信号转导通路和阿尔茨海默病及中药干预研究[J].中风与神经疾病杂志,2013,30(12):1136-1137.
[13]李锐,肖燕,和心依.等.中药姜黄化学成分、生物活性及体内代谢研究进展[J].西华大学学报:自然科学版,2013,32(3):98-104.
[14]夏秋香,白红彤,孙凌臣.等.药用植物母菊的成分和功能研究进展[J].园艺学报,2012,39(9):1859-1864.
[15]兰卫,郭玉婷,耿直.等.德国洋甘菊花序中总皂苷的含量测定[J].新疆医科大学学报,2014,37(3):315-316.
[16]申永春,杨婷,文富强.等.槲皮素对香烟暴露大鼠肺组织氧化应激和Nrf2表达的影响[J].西部医学,2011,23(11):2071-2073.
[17]常平,张颍,夏开元.等.红车轴草提取物中异黄酮成分的分析[J].食品科学,2007,28(9):449-452.
[18]张晓霞,周延萌,张小敏.等.红车轴草异黄酮药理作用的研究进展[J].中国医药指南,2012,10(11):102-104.
[19]侯成成,麻微微,肖荣.等.染料木黄铜对星形胶质细胞氧化损伤中Nrf2-ARE通路相关因子表达的调节作用[J].中国食品卫生杂志,2013,25(1):1-5.
[20]王素琴,汤玲君,王艳.芦丁对糖尿病肾病大鼠的保护作用及其机制探讨[J].医学研究杂志,2012,41(10):144-148.
[21]元海丹,李海燕,王瑜玲,等.芦丁对Hep G2肝癌细胞AMPK蛋白信号通路的激活作用[J].延边大学医学学报,2013,36(3):180-182.