雌激素受体与M2mAchR在成年雌兔背根神经节中的共存
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摘要
为检测成年雌兔背根神经节中是否存在雌激素受体(ER)与M2型乙酰胆碱受体(M2mAchR)的分布与共存,采用免疫荧光双标记染色法对ER与M2mAchR在背根神经节中的分布情况进行了研究。结果显示,在成年雌兔背根神经节中:(1) ER分布主要以神经元为主。其中,ERα主要表达于中小型神经元,而ERβ则多分布于大中型神经元。(2)相较ER而言,M2mAchR分布更为广泛,除神经元外,神经纤维等组织上也有弱阳性表达。(3)两种ER与M2mAchR均有共存现象,但共存情况存在差异,ERα/M2mAchR主要共分布于中小型细胞,ERβ/M2mAchR主要共分布于大中型神经元。结果表明,首先,雌激素具有通过影响背根神经节部分神经元的活动而实现对躯体内脏感觉活动间接调节的条件。其次,在此影响的机制中,雌激素除可以直接作用于神经元上的受体之外,还具有与共表达的M2mAchR交互作用而实现的条件。为研究雌激素影响神经系统调控活动的机制以及背根神经节的神经内分泌调节机制提供了重要的形态学依据。
Double immunofluorescence staining method were used to detect the distribution and co-expression of estrogen receptor(ER) and M2-acetylcholine receptor(M2 mAchR) in the dorsal root ganglion(DRG) of female adult rabbits.The results of immunofluorescence staining in DRG show that,firstly,ER were distributed in nerve cells mainly.Among them,small and medium-sized neurons were strong or medium positive to ERα.However,the cells with strong positive to ERβ,were observed in large and medium-sized neurons mostly,secondly,M2 mAchR immunopositivity was more widely distributtion than ER.Besides neurons,there were also weakly positive to others such as nerve fibers,finally,both types of ER and M2 mAchR were co-expressed in DRG.But the condition of these co-expressions were differenced,ERα importantly located in small and medium-sized neurons,and ERβ mostly in large and medium-sized neurons.These results suggested that estrogen may regulate the somatic and visceral sensory activity by affecting dorsal root ganglion neurons;in addition to immediately affect the ER in neurons,estrogen can also be achieved this role by acting on co-expression of M2 mAchR and ER.This result provides a morphological basis for studying the role of estrogen in regulating activity of nervous system through multiple pathways,and the neuroendocrine regulation mechanism of DRG.
Double immunofluorescence staining method were used to detect the distribution and co-expression of estrogen receptor(ER) and M2-acetylcholine receptor(M2 mAchR) in the dorsal root ganglion(DRG) of female adult rabbits.The results of immunofluorescence staining in DRG show that,firstly,ER were distributed in nerve cells mainly.Among them,small and medium-sized neurons were strong or medium positive to ERα.However,the cells with strong positive to ERβ,were observed in large and medium-sized neurons mostly,secondly,M2 mAchR immunopositivity was more widely distributtion than ER.Besides neurons,there were also weakly positive to others such as nerve fibers,finally,both types of ER and M2 mAchR were co-expressed in DRG.But the condition of these co-expressions were differenced,ERα importantly located in small and medium-sized neurons,and ERβ mostly in large and medium-sized neurons.These results suggested that estrogen may regulate the somatic and visceral sensory activity by affecting dorsal root ganglion neurons;in addition to immediately affect the ER in neurons,estrogen can also be achieved this role by acting on co-expression of M2 mAchR and ER.This result provides a morphological basis for studying the role of estrogen in regulating activity of nervous system through multiple pathways,and the neuroendocrine regulation mechanism of DRG.
引文
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[23] 滕可导,李莲军,陈耀星,等.山羊子宫内胆碱能神经分布及妊娠时的变化[J].中国兽医学报,2001,21(2):156-158.
[2] 郭晓,王志豪,吴卫妮,等.孕激素受体在雌性山羊腹腔肠系膜前神经节的分布[J].畜牧兽医学报,2013 (6):960-964.
[3] 董伟,范洁,徐永平,等.LH受体在雌性山羊肠系膜后神经节中的分布[J].西北农林科技大学学报(自然科学版),2015 (2):23-27.
[4] 孙曼.孕酮对ATP体外诱导仔兔感觉神经元内游离Ca2+浓度的变化影响[D].陕西杨凌:西北农林科技大学,2016.
[5] 董伟,黄双,徐永平,等.促黄体生成素受体在雌性山羊背根神经节中的表达分析[J].畜牧兽医学报,2014,(7):1175-1181.
[6] KWAKOWSKY A,MILNE M R,WALDVOGEL H J,et al.Effect of estradiol on neurotrophin receptors in basal forebrain cholinergic neurons:relevance for alzheimer’s disease[J].Int J Mol Sci,2016,17(12):e2122.
[7] Zhang Q G,Wang R,Tang H,et al.Brain-derived estrogen exerts anti-inflammatory and neuroprotective actions in the rat hippocampus[J].Mol Cell Endocrinol,2014,389(1):84-91.
[8] KIYAMA R,WADA-KIYAMA Y.Estrogenic endocrine disruptors:Molecular mechanisms of action[J].Environment International,2015,83:11-40.
[9] SAKIANI S,OLSEN N J,KOVACS W J,et al.Gonadal steroids and humoral immunity[J].Nat Rev Endocrinol,2013,9(1):56-62.
[10] LIMA R,WOFFORD M,RECKELHOFF J F.Hypertension in Postmenopausal Women[J].Curr Hypertens Rep,2012,14(3):254-260.
[11] 史雁暇,王志豪,李强,等.雌激素α、β受体在雌性山羊腹腔肠系膜前神经节的分布[J].西北农业学报,2014,(01):7-11.
[12] BONNER T I,BUCKLEY N J,YOUNG A C,et al.Identification of a family of muscarinic acetylcholine receptor genes[J].Science,1987,237(4814):527-532.
[13] TATA A M,VILARO M T,MENGOD G.Muscarinic receptor subtypes expression in rat and chick dorsal root ganglia[J].Mol Brain Res,2000,82(1):1-10.
[14] 唐占英,王拥军,周重建,等.不同大小背根节(DRG)神经元在痛觉调制中的作用[J].2005,(2):115-117.
[15] ROBERT E,STACIE K,TOTSCH,et al.Sex differences in pain[J].J Neuroscience Res,2017,95(6):1271-1281.
[16] DAWSON B M,GINTZLER A R.Gestational and ovarian sex steroid antinociception:synergy between spinal κ and δ opioid systems[J].Brain Re,1998,794(1):61-67.
[17] BEI M A,LI H Y,JUAN F,et al.Estrogen modulation of peripheral pain signal transduction:involvement of P2X(3) receptors[J].Purinergic Signal,2011,7(1):73-83.
[18] XIAO H S,HUANG Q H,ZHANG F X,et al.Identification of gene expression profile of dorsal root ganglion in the rat peripheral axotomy model of neuropathic pain[J].Proc Natl Acad Sci U S A,2002,99(12):8360-8365.
[19] DOUGLAS W W,RITCHIE J M.The excitatory action of acetylcholine on cutaneous non-myelinated fibres[J].J Physiol,1960,150(3):501-514.
[20] Steen K H,Reeh P W.Actions of cholinergic agonists and antagonists on sensory nerve endings in rat skin,in vitro[J].Pain,1990,70(01):397-405.
[21] HABERBERGER R,HENRICH M,COURAUD J Y,et al.Muscarinic M2-receptors in rat thoracic dorsal root ganglia[J].Neurosci Letters,1999,266(3):177-180.
[22] WESS J,DUTTAROY A,GOMEZA J,et al.Muscarinic receptor subtypes mediating central and peripheral antinociception studied with muscarinic receptor knockout mice:A review[J].Life Sci,2003,72(18):2047-2054.
[23] 滕可导,李莲军,陈耀星,等.山羊子宫内胆碱能神经分布及妊娠时的变化[J].中国兽医学报,2001,21(2):156-158.