广西地区艾滋病病毒感染/艾滋病患者蓝氏贾第鞭毛虫感染情况及基因型分析
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摘要
目的了解广西地区艾滋病病毒感染/艾滋病(HIV/AIDS)患者蓝氏贾第鞭毛虫(简称贾第虫)感染情况及基因型分析。方法收集广西地区HIV/AIDS患者和健康者粪样,镜检后提取粪样中贾第虫DNA,并采用巢式PCR方法扩增贾第虫磷酸丙糖异构酶基因(triosephosphate isomerase, TPI),对阳性结果进行测序和BLAST比对分析,并构建进化树。结果共收集285例HIV/AIDS患者粪样,其中男性216例,女性69例,年龄21～85岁,其中130例曾接受抗逆转录病毒治疗。HIV/AIDS患者粪样镜检贾第虫阳性8份,阳性率为2.8%;健康者粪样303份,无贾第虫感染,两者差异有统计学意义(P <0.01)。巢式PCR扩增阳性粪样,发现530 bp左右有一条特异性条带。经测序分析,有7份贾第虫样品为人兽共患集聚体B型,其中6份与已报到的人源分离株TPI基因序列一致性为100%。另外1份为集聚体C型,且该分离株与已报到的人源贾第虫集聚体C型基因序列一致性为100%。结论广西地区部分HIV/AIDS患者感染贾第虫,其基因型主要为集聚体B型。
Objective To investigate the co-infection rate and the genotype of Giardia lamblia among HIV/AIDS patients in Guangxi Autonomous Region. Methods Fecal specimens were collected from 285 HIV/AIDS patients and 303 HIV-negative people from Guangxi Autonomous Region. DNA were extracted from the feces and triosephosphate isomerase(TPI) gene of G. lamblia was amplified by nested PCR to determine the infection of G.lamblia. The amplified TPI PCR products were sequenced and the obtained sequences were aligned and analyzed by BLAST search. Results The overall co-infection rate of G. lamblia with HIV/AIDS was 2.8%(8/285). There was no G. lamblia infection detected in people without HIV-AIDS(P < 0.01). However, no significant risk factors were found to be related to the infection of G. lamblia among HIV-AIDS patients such as age, gender, sanitation,antiretroviral treatment or clinical manifestations(diarrhea, CD4+T lymphocyte level). Seven samples were identified to be zoonotic assemblage B and 6 had 100% sequence homology with the reported human-derived isolate. The other isolate was assemblage C and sequence identical to the reported human-derived isolate in our previous study.Conclusion HIV-AIDS patients have higher co-infection rate with G. lamblia than people without HIV infection.The major genotype of infected G. lamblia was assemblage B.
Objective To investigate the co-infection rate and the genotype of Giardia lamblia among HIV/AIDS patients in Guangxi Autonomous Region. Methods Fecal specimens were collected from 285 HIV/AIDS patients and 303 HIV-negative people from Guangxi Autonomous Region. DNA were extracted from the feces and triosephosphate isomerase(TPI) gene of G. lamblia was amplified by nested PCR to determine the infection of G.lamblia. The amplified TPI PCR products were sequenced and the obtained sequences were aligned and analyzed by BLAST search. Results The overall co-infection rate of G. lamblia with HIV/AIDS was 2.8%(8/285). There was no G. lamblia infection detected in people without HIV-AIDS(P < 0.01). However, no significant risk factors were found to be related to the infection of G. lamblia among HIV-AIDS patients such as age, gender, sanitation,antiretroviral treatment or clinical manifestations(diarrhea, CD4+T lymphocyte level). Seven samples were identified to be zoonotic assemblage B and 6 had 100% sequence homology with the reported human-derived isolate. The other isolate was assemblage C and sequence identical to the reported human-derived isolate in our previous study.Conclusion HIV-AIDS patients have higher co-infection rate with G. lamblia than people without HIV infection.The major genotype of infected G. lamblia was assemblage B.
引文
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[13] Liu H,Jiang Z,Yuan Z,et al. Infection by and genotype characteristics of Enterocytozoon bieneusi in HIV/AIDS patients from Guangxi Zhuang autonomous region,China[J]. BMC Infect Dis,2017,17(1):684.
[14] Sulaiman IM,Fayer R,Bern C,et al. Triosephosphate isomerase gene characterization and potential zoonotic transmission of Giardia duodenalis[J]. Emerg Infect Dis,2003,9(11):1444-1452.
[15] Liu H,Shen Y,Yin J,et al. Prevalence and genetic characterization of Cryptosporidium,Enterocytozoon,Giardia and Cyclospora in diarrheal outpatients in China[J]. BMC Infect Dis,2014,14(1):25.
[16]滕雪娇,陈家旭,田利光. HIV/AIDS患者合并肠道原虫感染状况[J].中国寄生虫学与寄生虫病杂志,2017,35(6):607-614.
[17] Li J,Wang H,Wang R,et al. Giardia duodenalis infections in humans and other animals in china[J]. Front Microbiol,2017,8:2004.
[18] Wegayehu T,Karim MR,Li J,et al. Multilocus genotyping of Giardia duodenalis isolates from children in Oromia Special Zone,central Ethiopia[J]. BMC Microbiol,2016,16:89.
[19] Sulaiman IM,Jiang J,Singh A,et al. Distribution of Giardia duodenalis genotypes and subtyes in raw urban wastewater in Milwa ukee,Wisconsin[J]. Appl Environ Microbiol,2004,70(6):3776-2780.
[20] Berrilli F,D′Alfonso R,Giangaspero A,et al. Giardia duodenalis genotypes Cryptosporidium species in humans and domestic animals in C觝te d′Ivoire:occurrence and evidence for environment contamination[J]. Trans R Soc Trop Med Hyg,2012,106(3):191-195.
[21] Brandonisio O,Maggi P,Panaro MA,et al. Intestinal protozoa in HIV-infected patients in Apulia,South Italy[J].Epidemiol Infect,1999,123(3):457-462.
[22] Nooshadokht M,Kalantari-Khandani B,Sharifi I,et al. Stool antigen immunodetection for diagnosis of Giardia duodenalis infection in human subjects with HIV and cancer[J]. J Microbiol Methods,2017,141:35-41.
[23] Lim YA,Iqbal A,Surin J,et al. First genetic classification of Cryptosporidium and Giardia from HIV/AIDS patients in Malaysia[J]. Infect Genet Evol,2011,11(5):968-974.
[24] Homan WL,Mank TG. Human giardiasis:genotype linked differences in clinical symptomatology[J]. Int J Parasitol,2001,31:822-826.
[2]詹希美.人体寄生虫学[M].北京:人民卫生出版社,2001:61.
[3]谢毅,郭兰英,宋琳,等.贾第虫病的实验室诊断[J].中国人兽共患病杂志,2002,18(6):101-103.
[4] Feng Y,Xiao L. Zoonotic potential and molecular epidemiology of Giardia species and giardiasis[J]. Clin Microbiol Rev,2011,24(1):110-140.
[5] Plutzer J,Ongerth J,Karanis P. Giardia taxonomy,phylogeny and epidemiology:facts and open questions[J]. Int J Hyg Environ Health,2010,213(5):321-333.
[6] Liu A,Yang F,Shen Y,et al. Genetic analysis of the Gdh and Bg genes of animal-derived Giardia duodenalis isolates in northeastern China and evaluation of zoonotic transmission potential[J]. PLoS One,2014,9(4):e95291.
[7] Liu H,Shen Y,Liu A,et al. Occurrence and multilocus genotyping of Giardia duodenalis in pets and zoo animals in Shanghai,China[J]. J Infect Dev Ctrie,2016,11(6):479-486.
[8]温少芳,卢思奇.蓝氏贾第鞭毛虫遗传多样性研究进展[J].国际医学寄生虫病杂志,2006,33(1):44-48.
[9] Lim YA,Iqbal A,Surin J,et al. First genetic classification of Cryptosporidium and Giardia from HIV/AIDS patients in Malaysia[J]. Infect Genet Evol,2011,11(5):968-974.
[10] Gon觭alves AC,Gabbay YB,Mascarenhas JD,et al. Calicivirus and Giardia lamblia are associated with diarrhea in human immunodeficiency virus-seropositive patients from southeast Brazil[J]. Am J Trop Med Hyg,2009,81(3):463-466.
[11] Akgül魻,Kart Y a覨s ar K,Sapmaz B,et al. Detection of intestinal parasites with conventional and molecular methods in follow-up HIV/AIDS cases[J]. Mikrobiyol Bul,2018,52(3):273-283.
[12]王尊付,蒋智华,于冰雪,等.广西HIV/AIDS患者合并感染隐孢子虫现况及基因分型初步研究[J].中国血吸虫病防治杂志,2016,28(5):550-553.
[13] Liu H,Jiang Z,Yuan Z,et al. Infection by and genotype characteristics of Enterocytozoon bieneusi in HIV/AIDS patients from Guangxi Zhuang autonomous region,China[J]. BMC Infect Dis,2017,17(1):684.
[14] Sulaiman IM,Fayer R,Bern C,et al. Triosephosphate isomerase gene characterization and potential zoonotic transmission of Giardia duodenalis[J]. Emerg Infect Dis,2003,9(11):1444-1452.
[15] Liu H,Shen Y,Yin J,et al. Prevalence and genetic characterization of Cryptosporidium,Enterocytozoon,Giardia and Cyclospora in diarrheal outpatients in China[J]. BMC Infect Dis,2014,14(1):25.
[16]滕雪娇,陈家旭,田利光. HIV/AIDS患者合并肠道原虫感染状况[J].中国寄生虫学与寄生虫病杂志,2017,35(6):607-614.
[17] Li J,Wang H,Wang R,et al. Giardia duodenalis infections in humans and other animals in china[J]. Front Microbiol,2017,8:2004.
[18] Wegayehu T,Karim MR,Li J,et al. Multilocus genotyping of Giardia duodenalis isolates from children in Oromia Special Zone,central Ethiopia[J]. BMC Microbiol,2016,16:89.
[19] Sulaiman IM,Jiang J,Singh A,et al. Distribution of Giardia duodenalis genotypes and subtyes in raw urban wastewater in Milwa ukee,Wisconsin[J]. Appl Environ Microbiol,2004,70(6):3776-2780.
[20] Berrilli F,D′Alfonso R,Giangaspero A,et al. Giardia duodenalis genotypes Cryptosporidium species in humans and domestic animals in C觝te d′Ivoire:occurrence and evidence for environment contamination[J]. Trans R Soc Trop Med Hyg,2012,106(3):191-195.
[21] Brandonisio O,Maggi P,Panaro MA,et al. Intestinal protozoa in HIV-infected patients in Apulia,South Italy[J].Epidemiol Infect,1999,123(3):457-462.
[22] Nooshadokht M,Kalantari-Khandani B,Sharifi I,et al. Stool antigen immunodetection for diagnosis of Giardia duodenalis infection in human subjects with HIV and cancer[J]. J Microbiol Methods,2017,141:35-41.
[23] Lim YA,Iqbal A,Surin J,et al. First genetic classification of Cryptosporidium and Giardia from HIV/AIDS patients in Malaysia[J]. Infect Genet Evol,2011,11(5):968-974.
[24] Homan WL,Mank TG. Human giardiasis:genotype linked differences in clinical symptomatology[J]. Int J Parasitol,2001,31:822-826.