水稻不育系‘培矮64S’空间搭载的“双低”选育与应用
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摘要
【目的】解决水稻Oryza sativa L.两系不育系‘培矮64S’所面临的直链淀粉含量偏高和不育起点温度因遗传漂移而不断上升的问题,选育新的两系不育系。【方法】通过利用‘培矮64S’种子搭载"实践八号"农业卫星、地面种植和跟踪、定向筛选,筛选到具有低直链淀粉含量的稳定不育株系,同时结合水稻光温敏不育系的提纯和原种生产方法,对不育起点温度进行加压选择与鉴定。【结果】育成具有低直链淀粉含量(w为8.69%左右)、低不育起点温度(23.0℃以下)的新型"双低"不育系,命名为‘航17S’。该不育系株高79.62 cm,剑叶长32.54 cm、宽1.82 cm,有效穗数11.4个,穗长22.01 cm,每穗总粒数约181粒。除剑叶宽以外,其他主要性状均与野生型‘培矮64S’没有显著差异。【结论】‘航17S’除了具有"双低"特点以外,其他主要农艺性状表现与野生型‘培矮64S’基本一致,而且保留了‘培矮64S’的异交特性好、配合力强等优良特点,于2015年10月通过了广东省技术鉴定。
【Objective】To solve the problems of thermo-photo-sensitive genic male sterile(PTGMS) rice ‘Peiai 64 S' caused by rising critical sterility-inducing temperature(CSIT) and higher amylose content in practical production, and breed new male sterile line.【Method】‘Peiai 64 S' seeds, which were carried by the agricultural satellite named as "practical #8", were planted, tracked and directionally selected. The steady sterile line with low amylose content was obtained. In the mean time, according to YUAN Longping's method for the purification and propagation of PTGMS lines, CSIT was selected and identified by adding pressure.【Result】The mutant plants with low amylose content(about 8.69%) and low CSIT(below 23.0 ℃) were found. One new mutant line with the dual-low characteristics was identified and named as ‘Hang 17 S'. The plant height of ‘Hang 17 S' was 79.62 cm, the flag leaf length and width were 32.54 and 1.82 cm respectively, the panicle number per plant was 11.4, the panicle length was 22.01 cm and the grain number per panicle was about 181. Except for the flag leaf width, there was no significant difference for most of the traits compared with its wild type ‘Peiai 64 S'.【Conclusion】The newly bred ‘Hang 17 S' is much like its wild type ‘Peiai 64 S' for most of the traits except for the dual-low characteristics. And it retains the characteristics of good outcrossing rate and strong combining ability of ‘Peiai 64 S'. ‘Hang 17 S' was appraised by the government committee and commercially released in 2015.
【Objective】To solve the problems of thermo-photo-sensitive genic male sterile(PTGMS) rice ‘Peiai 64 S' caused by rising critical sterility-inducing temperature(CSIT) and higher amylose content in practical production, and breed new male sterile line.【Method】‘Peiai 64 S' seeds, which were carried by the agricultural satellite named as "practical #8", were planted, tracked and directionally selected. The steady sterile line with low amylose content was obtained. In the mean time, according to YUAN Longping's method for the purification and propagation of PTGMS lines, CSIT was selected and identified by adding pressure.【Result】The mutant plants with low amylose content(about 8.69%) and low CSIT(below 23.0 ℃) were found. One new mutant line with the dual-low characteristics was identified and named as ‘Hang 17 S'. The plant height of ‘Hang 17 S' was 79.62 cm, the flag leaf length and width were 32.54 and 1.82 cm respectively, the panicle number per plant was 11.4, the panicle length was 22.01 cm and the grain number per panicle was about 181. Except for the flag leaf width, there was no significant difference for most of the traits compared with its wild type ‘Peiai 64 S'.【Conclusion】The newly bred ‘Hang 17 S' is much like its wild type ‘Peiai 64 S' for most of the traits except for the dual-low characteristics. And it retains the characteristics of good outcrossing rate and strong combining ability of ‘Peiai 64 S'. ‘Hang 17 S' was appraised by the government committee and commercially released in 2015.
引文
[1]石明松.晚粳自然两用系的选育及应用初报[J].湖北农业科学,1981(7):1-3.
[2]石明松.对光照长度敏感的隐性雄性不育水稻的发现与初步研究[J].中国农业科学,1985,18(2):44-48.
[3]袁隆平.杂交水稻的育种战略设想[J].杂交水稻,1987,2(1):1-3.
[4]王丰,李永辉,柳武革,等.水稻不育系培矮64S的空间诱变效应及后代的SSR分析[J].核农学报,2006,20(6):449-453.
[5]邓启云,符习勤.光温敏核不育水稻育性稳定性研究:Ⅲ:不育起点温度漂移及其控制技术[J].湖南农业大学学报,1998,24(1):11-16.
[6]段美娟,袁定阳,邓启云,等.光温敏核不育水稻育性稳定性研究:Ⅳ:不育起点温度漂移规律[J].杂交水稻,2003,18(2):64-66.
[7]郭涛,纪庆绵,陈志强,等.籼型水稻突变体MLA-1低直链淀粉突变基因遗传分析[C]//李东辉.2007年全国作物遗传育种学术研讨会论文集.北京:中国作物学会,2007:16-20.
[8]郭涛,纪庆绵,陈志强,等.籼型水稻突变体MLA-1低直链淀粉突变基因遗传分析[J].湖南农业大学学报(自然科学版),2007,33(S1):16-20.
[9]郭涛,韦璇,王慧,等.2个低直链淀粉含量籼稻突变体的遗传分析[J].华南农业大学学报,2009,30(1):10-13.
[10]刘永柱,陈志强,张建国,等.空间诱变水稻广谱恢复系航恢七号的选育及利用[J].核农学报,2008,22(4):439-442.
[11]夏斌,郭涛,王慧,等.2个籼稻低直链淀粉突变体淀粉合成关键酶分析[J].华南农业大学学报,2011,32(3):10-13.
[12]袁隆平.水稻光温敏不育系的提纯和原种生产[J].杂交水稻,1994,9(6):1-3.
[13]黄翠红,刘永柱,陈立凯,等.水稻培矮64S空间诱变突变株系的花培效应研究[J].核农学报,2014,28(3):386-392.
[14]赵国珍,贾育林,严宗卜,等.一种高效便捷的水稻DNA提取法及其应用[J].中国水稻科学,2012,26(4):495.
[15]张建勇,陈德全,李仕贵,等.利用分子标记鉴定水稻品种的Wx等位基因及其与直链淀粉含量的关系[J].作物学报,2005,31(4):420.
[16]LUO W L,GUO T,YANG Q Y,et al.Stacking of five favorable alleles for amylase content,fragrance and disease resistance into elite lines in rice(Oryza sativa)by using four HRM-based markers and a linked gel-based marker[J].Mol Breed,2014,34(3):805-815.
[17]罗文龙,郭涛,周丹华,等.利用基于HRM的功能标记分析水稻Wx和fgr的基因型[J].湖南农业大学学报(自然科学版),2013,39(6):597.
[18]国家质量技术监督局.优质稻谷:GB/TM891-1999[S].北京:中国标准出版社,2000.
[19]中华人民共和国农业部.水稻光、温敏雄性核不育系育性鉴定规程:NY/T 1215-2006[S].北京:中国农业出版社,2007.
[20]舒庆尧,吴殿星,夏英武,等.籼稻和粳稻中蜡质基因座位上微卫星标记的多态性及其与直链淀粉含量的关系[J].遗传学报,1999,26(4):350-358.
[21]谈移芳,张启发.水稻蜡质基因引导区两个SSR序列与直链淀粉含量的相关性[J].植物学报,2001,43(2):146-150.
[22]万向元,胡培松,王海莲,等.水稻品种直链淀粉含量、糊化温度和蛋白质含量的稳定性分析[J].中国农业科学,2005,38(1):1-6.
[23]WANG Z Y,WU Z L,XING Y Y,et al.Nucleotide sequence of rice waxy gene[J].Nucleic Acids Res,1990,18(19):5898.
[24]MIKAMI I,UWATOKO N,IKEDA Y,et al.Allelic diversification at the wx locus in landraces of Asian rice[J].Theor Appl Genet,2008,116(7):979-989.
[25]何风华,曾瑞珍,席章营,等.不同Waxy基因型水稻的遗传多样性[J].分子植物育种,2003,1(2):179-186.
[26]朱昌兰,沈文飚,翟虎渠,等.水稻低直链淀粉含量基因育种利用的研究进展[J].中国农业科学,2004,37(2):157-162.
[27]罗孝和.“培矮64S”核不育系标准[J].杂交水稻,2000,15(S2):134-135.
[28]于永红,斯华敏,胡国成,等.不同来源培矮64S育性转换光温反应特性的分析[J].中国农业科学,2006,39(5):1064-1068.
[29]程式华,孙宗修,斯华敏.人工控制条件下水稻光(温)敏核不育系育性转换的整齐度分析[J].浙江农业学报,1993,5(3):133-137.
[30]徐孟亮,周广洽,陈良碧.培矮64S育性对温度与光周期的反应[J].作物学报,1999,25(6):772-776.
[2]石明松.对光照长度敏感的隐性雄性不育水稻的发现与初步研究[J].中国农业科学,1985,18(2):44-48.
[3]袁隆平.杂交水稻的育种战略设想[J].杂交水稻,1987,2(1):1-3.
[4]王丰,李永辉,柳武革,等.水稻不育系培矮64S的空间诱变效应及后代的SSR分析[J].核农学报,2006,20(6):449-453.
[5]邓启云,符习勤.光温敏核不育水稻育性稳定性研究:Ⅲ:不育起点温度漂移及其控制技术[J].湖南农业大学学报,1998,24(1):11-16.
[6]段美娟,袁定阳,邓启云,等.光温敏核不育水稻育性稳定性研究:Ⅳ:不育起点温度漂移规律[J].杂交水稻,2003,18(2):64-66.
[7]郭涛,纪庆绵,陈志强,等.籼型水稻突变体MLA-1低直链淀粉突变基因遗传分析[C]//李东辉.2007年全国作物遗传育种学术研讨会论文集.北京:中国作物学会,2007:16-20.
[8]郭涛,纪庆绵,陈志强,等.籼型水稻突变体MLA-1低直链淀粉突变基因遗传分析[J].湖南农业大学学报(自然科学版),2007,33(S1):16-20.
[9]郭涛,韦璇,王慧,等.2个低直链淀粉含量籼稻突变体的遗传分析[J].华南农业大学学报,2009,30(1):10-13.
[10]刘永柱,陈志强,张建国,等.空间诱变水稻广谱恢复系航恢七号的选育及利用[J].核农学报,2008,22(4):439-442.
[11]夏斌,郭涛,王慧,等.2个籼稻低直链淀粉突变体淀粉合成关键酶分析[J].华南农业大学学报,2011,32(3):10-13.
[12]袁隆平.水稻光温敏不育系的提纯和原种生产[J].杂交水稻,1994,9(6):1-3.
[13]黄翠红,刘永柱,陈立凯,等.水稻培矮64S空间诱变突变株系的花培效应研究[J].核农学报,2014,28(3):386-392.
[14]赵国珍,贾育林,严宗卜,等.一种高效便捷的水稻DNA提取法及其应用[J].中国水稻科学,2012,26(4):495.
[15]张建勇,陈德全,李仕贵,等.利用分子标记鉴定水稻品种的Wx等位基因及其与直链淀粉含量的关系[J].作物学报,2005,31(4):420.
[16]LUO W L,GUO T,YANG Q Y,et al.Stacking of five favorable alleles for amylase content,fragrance and disease resistance into elite lines in rice(Oryza sativa)by using four HRM-based markers and a linked gel-based marker[J].Mol Breed,2014,34(3):805-815.
[17]罗文龙,郭涛,周丹华,等.利用基于HRM的功能标记分析水稻Wx和fgr的基因型[J].湖南农业大学学报(自然科学版),2013,39(6):597.
[18]国家质量技术监督局.优质稻谷:GB/TM891-1999[S].北京:中国标准出版社,2000.
[19]中华人民共和国农业部.水稻光、温敏雄性核不育系育性鉴定规程:NY/T 1215-2006[S].北京:中国农业出版社,2007.
[20]舒庆尧,吴殿星,夏英武,等.籼稻和粳稻中蜡质基因座位上微卫星标记的多态性及其与直链淀粉含量的关系[J].遗传学报,1999,26(4):350-358.
[21]谈移芳,张启发.水稻蜡质基因引导区两个SSR序列与直链淀粉含量的相关性[J].植物学报,2001,43(2):146-150.
[22]万向元,胡培松,王海莲,等.水稻品种直链淀粉含量、糊化温度和蛋白质含量的稳定性分析[J].中国农业科学,2005,38(1):1-6.
[23]WANG Z Y,WU Z L,XING Y Y,et al.Nucleotide sequence of rice waxy gene[J].Nucleic Acids Res,1990,18(19):5898.
[24]MIKAMI I,UWATOKO N,IKEDA Y,et al.Allelic diversification at the wx locus in landraces of Asian rice[J].Theor Appl Genet,2008,116(7):979-989.
[25]何风华,曾瑞珍,席章营,等.不同Waxy基因型水稻的遗传多样性[J].分子植物育种,2003,1(2):179-186.
[26]朱昌兰,沈文飚,翟虎渠,等.水稻低直链淀粉含量基因育种利用的研究进展[J].中国农业科学,2004,37(2):157-162.
[27]罗孝和.“培矮64S”核不育系标准[J].杂交水稻,2000,15(S2):134-135.
[28]于永红,斯华敏,胡国成,等.不同来源培矮64S育性转换光温反应特性的分析[J].中国农业科学,2006,39(5):1064-1068.
[29]程式华,孙宗修,斯华敏.人工控制条件下水稻光(温)敏核不育系育性转换的整齐度分析[J].浙江农业学报,1993,5(3):133-137.
[30]徐孟亮,周广洽,陈良碧.培矮64S育性对温度与光周期的反应[J].作物学报,1999,25(6):772-776.