UPC~2法同时测定乳品中2种着色剂
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摘要
建立了超高效合相色谱(Ultra Performance Convergence Chromatography, UPC~2)法同时测定奶粉中β-胡萝卜素和叶黄素的方法。超高效合相色谱(UPC~2)技术集合超临界流体色谱(Supercritical Fluid Chromatography, SFC)和超高效液相色谱(Ultra Performance Liquid Chromatography, UPLC~(TM))的技术优点,流动相CO_2为主体,乙醇/乙腈(1︰1)为助溶剂。选用ACQUITY UPC~2 BEH 2-EP色谱柱,流速为0.8 mL/min,检测波长为438 nm,上述2种类胡萝卜素在0.025 6～2.636 8μg/m L范围内有良好的线性关系,相关系数R~2均大于0.998,在0.8～1.8 mg/kg范围内平均回收率在89.8%～98.7%之间,每个加标水平的6次重复试验RSD<7.8%。试验表明该方法简便、快速、准确,可同时测定乳品中的叶黄素和β-胡萝卜素含量。
A new method was developed for the determination of lutein and β-carotene in milk products by Ultra Performance Convergence Chromatography(UPC~2). The UPC~2 was mixture with Supercritical Fluid Chromatography and Ultra Performance Liquid Chromatography, mobile phase CO_2 as the main body, ethanol/acetonitrile(1︰1) as the cosolvent, methanol at a flow rate of 0.8 mL/min. Using the ACQUITY UPC~2 BEH 2-EP column, the UV detector was set at a wavelength of 438 nm. The linearities for two carotenoids were good at concentration ranges of 0.025 6-2.636 8 μg/mL, and the correlation coefficients were greater than 0.998. The average spiked recoveries for three addition levels were from 89.8% to 98.7%. The relative standard deviation of each spiked test was less than 7.8%. The method could simultaneously determine lutein and β-carotene levels in powdered milk with simpleness, speediness and accuracy.
A new method was developed for the determination of lutein and β-carotene in milk products by Ultra Performance Convergence Chromatography(UPC~2). The UPC~2 was mixture with Supercritical Fluid Chromatography and Ultra Performance Liquid Chromatography, mobile phase CO_2 as the main body, ethanol/acetonitrile(1︰1) as the cosolvent, methanol at a flow rate of 0.8 mL/min. Using the ACQUITY UPC~2 BEH 2-EP column, the UV detector was set at a wavelength of 438 nm. The linearities for two carotenoids were good at concentration ranges of 0.025 6-2.636 8 μg/mL, and the correlation coefficients were greater than 0.998. The average spiked recoveries for three addition levels were from 89.8% to 98.7%. The relative standard deviation of each spiked test was less than 7.8%. The method could simultaneously determine lutein and β-carotene levels in powdered milk with simpleness, speediness and accuracy.
引文
[1]惠伯棣.类胡萝卜素化学及生物化学[M].北京:中国轻工业出版社, 2005:2-3.
[2]韩雅珊.类胡萝卜素的功能研究进展[J].中国农业大学学报, 1999, 4(1):5-9.
[3]朱海霞,郑建仙.叶黄素(Lutein)的结构、分布、物化性质及生理功能[J].中国食品添加剂, 2005(5):48-55.
[4] KARADAS F, PAPPAS A C, SURAI P F, et al. Emobryonic development within carotenoid-enriched eggs influences the post-hatch carotenoid status of the chicken[J]. Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology, 2005, 141:244-251.
[5] GERMAIN P, CHAMBON P, EICHELE G, et al. International union of pharmacology. LXIII. RetinoidX receptors[J].Pharmacological Reviews, 2006, 58:760-772.
[6] KRINSKY N I, JOHNSON E J. Carotenoid actions and their relation to health and disease[J]. Molecular Aspects of Medicine, 2005, 26:459-516.
[7] NISHINO H, MURAKOSHI M, TOKUDA H, et al. Arch Biochem Biophy[J]. 2009, 483(2):165-168.
[8]孙玉敬,乔丽萍,钟烈洲,等.类胡萝卜素生物活性的研究进展[J].中国食品学报, 2012, 12(1):160-163.
[9] KARPPI J, KURL S, MAKIKALLIO T H, et al. Serumβ-caroteneconcentrationsandtheriskofcongestive heart failure in men:A population-based study[J]. Int J Cardio, 2013, 168(3):1841-1846.
[10]中华人民共和国卫生部.食品营养强化剂使用标准:GB14880—2012[S].北京:中国标准出版社, 2012.
[11]姚建花,黄百芬,任一平,等.高效液相色谱法同时测定果蔬中五种类胡萝卜素[J].营养学报, 2013, 34(1):282-288.
[12]王旻,钱永军,鄂来明.营养强化剂和婴儿配方奶粉中β-胡萝卜素的测定[J].中国乳品工业, 2005, 33(6):56-57.
[13] BRENDON D G, HARVEY E I. Liquid chromatographic method for the determination of lutein in milk and pediatric formulas[J]. Inter-national Dairy Journal, 2008, 18(9):894-898.
[14]黄菲菲,赵嘉胤.乳与乳制品中叶黄素的测定-高效液相色谱法[J].中国乳品工业, 2010, 38(6):54-59.
[15]涂一名,夏虹,杨洁,等.反相高效液相色谱法测定强化奶粉中β-胡萝卜素含量[J].分析测试技术与仪器, 2006,12(1):15-19.
[16] LUTEROTTI S, FRANKO M, BICANIC D, et al.Ultrasensitive determination of beta-carotene in fish oilbased supplementary drugs by HPLC-TLS[J]. Journal of Pharmceutical and Biomedical Analysis, 1999, 21(5):901-909.
[17] KELM M A, FLANAGAN V P, PAWLOSKY R J, et al.Quantitative detemination of 13C-labeled and endogenousβ-carotene, lutein, and vitamin A in human plasma[J]. Lipids,2001, 36(11):1277.
[18] WILLS R B H, RANGGA A. Determination of carotenoids in Chinese vegetables[J]. Food Chemistry, 1996, 56(4):451.
[19] GRAND-GUILLAUME PERRENOUD A, BOCCARD J, VEUTJEY J L, et al. Analysis of basic compounds by supercritical fluid chromatography:attempts to improve peak shape and maintain mass spectrometry compatibility[J]. Journal of Chromatography A, 2012, 1262:205-213.
[20]BERRYAJ,GAMESDE,PERKINSJR.Supercritical fluid chromatographic and supercritical fluid chromatographic-mass spectrometric studies of some polar compounds[J]. Journal of Chromatography A, 1986, 363(3):147-158.
[2]韩雅珊.类胡萝卜素的功能研究进展[J].中国农业大学学报, 1999, 4(1):5-9.
[3]朱海霞,郑建仙.叶黄素(Lutein)的结构、分布、物化性质及生理功能[J].中国食品添加剂, 2005(5):48-55.
[4] KARADAS F, PAPPAS A C, SURAI P F, et al. Emobryonic development within carotenoid-enriched eggs influences the post-hatch carotenoid status of the chicken[J]. Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology, 2005, 141:244-251.
[5] GERMAIN P, CHAMBON P, EICHELE G, et al. International union of pharmacology. LXIII. RetinoidX receptors[J].Pharmacological Reviews, 2006, 58:760-772.
[6] KRINSKY N I, JOHNSON E J. Carotenoid actions and their relation to health and disease[J]. Molecular Aspects of Medicine, 2005, 26:459-516.
[7] NISHINO H, MURAKOSHI M, TOKUDA H, et al. Arch Biochem Biophy[J]. 2009, 483(2):165-168.
[8]孙玉敬,乔丽萍,钟烈洲,等.类胡萝卜素生物活性的研究进展[J].中国食品学报, 2012, 12(1):160-163.
[9] KARPPI J, KURL S, MAKIKALLIO T H, et al. Serumβ-caroteneconcentrationsandtheriskofcongestive heart failure in men:A population-based study[J]. Int J Cardio, 2013, 168(3):1841-1846.
[10]中华人民共和国卫生部.食品营养强化剂使用标准:GB14880—2012[S].北京:中国标准出版社, 2012.
[11]姚建花,黄百芬,任一平,等.高效液相色谱法同时测定果蔬中五种类胡萝卜素[J].营养学报, 2013, 34(1):282-288.
[12]王旻,钱永军,鄂来明.营养强化剂和婴儿配方奶粉中β-胡萝卜素的测定[J].中国乳品工业, 2005, 33(6):56-57.
[13] BRENDON D G, HARVEY E I. Liquid chromatographic method for the determination of lutein in milk and pediatric formulas[J]. Inter-national Dairy Journal, 2008, 18(9):894-898.
[14]黄菲菲,赵嘉胤.乳与乳制品中叶黄素的测定-高效液相色谱法[J].中国乳品工业, 2010, 38(6):54-59.
[15]涂一名,夏虹,杨洁,等.反相高效液相色谱法测定强化奶粉中β-胡萝卜素含量[J].分析测试技术与仪器, 2006,12(1):15-19.
[16] LUTEROTTI S, FRANKO M, BICANIC D, et al.Ultrasensitive determination of beta-carotene in fish oilbased supplementary drugs by HPLC-TLS[J]. Journal of Pharmceutical and Biomedical Analysis, 1999, 21(5):901-909.
[17] KELM M A, FLANAGAN V P, PAWLOSKY R J, et al.Quantitative detemination of 13C-labeled and endogenousβ-carotene, lutein, and vitamin A in human plasma[J]. Lipids,2001, 36(11):1277.
[18] WILLS R B H, RANGGA A. Determination of carotenoids in Chinese vegetables[J]. Food Chemistry, 1996, 56(4):451.
[19] GRAND-GUILLAUME PERRENOUD A, BOCCARD J, VEUTJEY J L, et al. Analysis of basic compounds by supercritical fluid chromatography:attempts to improve peak shape and maintain mass spectrometry compatibility[J]. Journal of Chromatography A, 2012, 1262:205-213.
[20]BERRYAJ,GAMESDE,PERKINSJR.Supercritical fluid chromatographic and supercritical fluid chromatographic-mass spectrometric studies of some polar compounds[J]. Journal of Chromatography A, 1986, 363(3):147-158.