食品中合成色素检测方法的改进
|
摘要
通过高效液相色谱法(high performance liquid chromatography,HPLC)测定食品合成着色剂的条件的研究,得出高效液相色谱法同时测定9种合成着色剂的最佳条件。试验发现,利用固相萃取柱快速检测食品中的合成色素,可同时测定9种色素(新红、柠檬黄、苋菜红、靛蓝、胭脂红、日落黄、亮蓝、赤藓红、诱惑红),经过甲醇和0.02 mol/L乙酸铵梯度洗脱,流速1.0 mL/min时,采用紫外检测器,波长254 nm下进行检测。通过加标回收,计算回收率、相对标准偏差,各组分浓度与峰面积呈现良好的线性关系。通过对多种类型食品的实际检测,验证该方法可作为食品检测9种合成色素的检测方法。
It cloud get the best conditions for simultaneous determination of 9 synthetic colorants,by the research of high performance liquid chromatography(HPLC)determining synthetic colorants. The experiment showed it cloud determinate 9 kinds of pigment(red,tartrazine,amaranth,indigo,carmine,sunset yellow,bright blue,erythrosine,temptation red),using solid phase extraction column rapid detection of synthetic pigment in food,with methanol and 0.02 mol/L ammonium acetate gradient elution,and the flow rate of1.0 mL/min,with ultraviolet detector,detection under the wavelength of 254 nm. Through standard recovery,recovery rate and relative standard deviation,the concentration of each component had a good linear relationship with the peak area. Through the actual detection of various types of food,it verified that this method can be used as the detection method for 9 kinds of synthetic pigments in food detection.
It cloud get the best conditions for simultaneous determination of 9 synthetic colorants,by the research of high performance liquid chromatography(HPLC)determining synthetic colorants. The experiment showed it cloud determinate 9 kinds of pigment(red,tartrazine,amaranth,indigo,carmine,sunset yellow,bright blue,erythrosine,temptation red),using solid phase extraction column rapid detection of synthetic pigment in food,with methanol and 0.02 mol/L ammonium acetate gradient elution,and the flow rate of1.0 mL/min,with ultraviolet detector,detection under the wavelength of 254 nm. Through standard recovery,recovery rate and relative standard deviation,the concentration of each component had a good linear relationship with the peak area. Through the actual detection of various types of food,it verified that this method can be used as the detection method for 9 kinds of synthetic pigments in food detection.
引文
[1]戚平,刘佳,毛新武,等.食品中色素检测的研究进展[J].食品与机械, 2018,34(11):167-173
[2]中华人民共和国卫生和计划生育委员会.食品安全国家标准食品中合成着色剂的测定:GB 5009.35-2016[S].北京:中国标准出版社,2016
[3]中华人民公共和国出入境检验检疫行业标准.食品中诱惑红、酸性红、亮蓝、日落黄的含量检测高效液相色谱法:SN/T 1743-2006[S].北京:中国标准出版社,2006
[4]李小云,赵喜玲,李琪,等.食品中10种色素的高效液相色谱同时检测方法[J].四川师范大学学报(自然科学版), 2016,39(6):895-899
[5]夏宗艳,徐文远,徐豪,等.超高压液相色谱同时检测食品中8种人工合成色素[J].化工技术与开发,2019,48(1):52-56
[6]林芳,李涛,王一欣,等.固相萃取-超高效液相色谱法同时测定蜜饯中9种合成色素[J].食品安全质量检测学报,2016,7(2):567-574
[7]黎星,江丰,王会霞,等.固相萃取-超高效液相色谱法测定食品中10种合成色素[J].食品工业, 2018, 39(10):325-328
[8]戴玉婷,杨晋青,葛淑丽,等.食品与发酵科技,固相萃取-高效液相色谱法测定饮料中的9种合成着色剂[J].食品与发酵科技,2019,55(2):81-84
[9]付大友,袁东,张小芳.高效液相色谱同时测定葡萄酒中的合成色素[J].食品研究与开发, 2016,37(22):134-138
[10]中华人民共和国国家卫生和计划生育委员会.食品安全国家标准食品添加剂使用标准:GB 2760-2014[S].北京:中国标准出版社,2014
[2]中华人民共和国卫生和计划生育委员会.食品安全国家标准食品中合成着色剂的测定:GB 5009.35-2016[S].北京:中国标准出版社,2016
[3]中华人民公共和国出入境检验检疫行业标准.食品中诱惑红、酸性红、亮蓝、日落黄的含量检测高效液相色谱法:SN/T 1743-2006[S].北京:中国标准出版社,2006
[4]李小云,赵喜玲,李琪,等.食品中10种色素的高效液相色谱同时检测方法[J].四川师范大学学报(自然科学版), 2016,39(6):895-899
[5]夏宗艳,徐文远,徐豪,等.超高压液相色谱同时检测食品中8种人工合成色素[J].化工技术与开发,2019,48(1):52-56
[6]林芳,李涛,王一欣,等.固相萃取-超高效液相色谱法同时测定蜜饯中9种合成色素[J].食品安全质量检测学报,2016,7(2):567-574
[7]黎星,江丰,王会霞,等.固相萃取-超高效液相色谱法测定食品中10种合成色素[J].食品工业, 2018, 39(10):325-328
[8]戴玉婷,杨晋青,葛淑丽,等.食品与发酵科技,固相萃取-高效液相色谱法测定饮料中的9种合成着色剂[J].食品与发酵科技,2019,55(2):81-84
[9]付大友,袁东,张小芳.高效液相色谱同时测定葡萄酒中的合成色素[J].食品研究与开发, 2016,37(22):134-138
[10]中华人民共和国国家卫生和计划生育委员会.食品安全国家标准食品添加剂使用标准:GB 2760-2014[S].北京:中国标准出版社,2014