珍稀药用植物黑蒴育苗试验
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摘要
[目的]研究黑蒴育苗方法。[方法]应用正交试验设计的方法,从播种基质、水分、播种深度和种子处理4个因素出发,探讨对黑蒴出苗率的影响。[结果]种子处理对播种育苗试验结果的影响最大,其次是播种深度影响较大,其余2个因素对播种育苗存在一定的影响,但差异性均较小;通过试验结果分析选择出最佳的播种育苗方案为:以珍珠岩与红壤土作为栽培基质,3 d浇一次水,播种深度0.3 cm,种子用自来水浸泡24 h后进行播种最好。[结论]该研究为黑蒴的育苗提供理论依据和技术支持。
[Objective] The research aimed to study the method of seedling cultivation of Melasma arvense. [Method] The orthogonal test design method was applied to explore the influence of four factors, namely, sowing media, water content, depth of seeding and seed treatment on the emergence rate of Melasma arvense seedlings. [Result] Seed treatment had the most significant effect on the seedling cultivation followed by depth of seedling. The differences of other factors were insignificant. Through an analysis of the experiment results, the optimal program for sowing and seedling cultivation was selected and shown as follows: taking perlite and red soils as the cultivation medium, water the seed covered with 0.3 cm-thick sand every three days, and the optimal timing to sow the seed was 24 hours after it was soaked in tap water. [Conclusion] This study provides theoretical basis and technical support for seedling of Melasma arvense.
[Objective] The research aimed to study the method of seedling cultivation of Melasma arvense. [Method] The orthogonal test design method was applied to explore the influence of four factors, namely, sowing media, water content, depth of seeding and seed treatment on the emergence rate of Melasma arvense seedlings. [Result] Seed treatment had the most significant effect on the seedling cultivation followed by depth of seedling. The differences of other factors were insignificant. Through an analysis of the experiment results, the optimal program for sowing and seedling cultivation was selected and shown as follows: taking perlite and red soils as the cultivation medium, water the seed covered with 0.3 cm-thick sand every three days, and the optimal timing to sow the seed was 24 hours after it was soaked in tap water. [Conclusion] This study provides theoretical basis and technical support for seedling of Melasma arvense.
引文
[1] 中国科学院《中国植物志》编辑委员会.中国植物志:第67卷第2分册[M].北京:科学出版社,1996:350.
[2] 国家医药管理局中草药情报中心站.植物药有效成分手册[M].北京:人民卫生出版社,1986:102-103.
[3] 国家中医药管理局,《中华本草》编委会.中华本草:第七部第20卷[M].上海:上海科学技术出版社,1995:353.
[4] 云南省思茅地区革命委员会文卫组.云南思茅中草药选[M].云南:云南省思茅地区革命委员会,1971:162.
[5] 徐叔云,卞如镰,陈修.药理实验方法学[M].3版.北京:人民卫生出版社,2012:1203.
[6] 陈清华,莫莉,袁肇凯,等.养心通脉方有效成分部位抗心肌缺血的实验研究[J].中华中医药学刊,2008,26(9):1910-1913.
[7] 明磊,卢丹,刘金平,等.抗心肌缺血中药有效成分研究进展[J].人参研究,2010(3):38-42.
[8] 杨仁洲,周俊,郑启泰,等.一个新奇的单萜甙——黑蒴甙[J].云南植物研究,1983,5(2):215-218.
[9] 杨仁洲,周俊.黑蒴中的单萜甙类化合物[J].云南植物研究,1987,9(1):103-107.
[10] 苏成业,杨彤,曹士宁,等.七种中草药的不同提取物对动物移植性肿瘤的影响[J].遵义医学院学报,1995,5(2):31-33,39.
[11] 李育川,房海灵,王定康,等.云南珍稀民族药黑蒴茎芽组织培养快繁体系研究[J].时珍国医国药,2016,27(11):2746-2749.
[2] 国家医药管理局中草药情报中心站.植物药有效成分手册[M].北京:人民卫生出版社,1986:102-103.
[3] 国家中医药管理局,《中华本草》编委会.中华本草:第七部第20卷[M].上海:上海科学技术出版社,1995:353.
[4] 云南省思茅地区革命委员会文卫组.云南思茅中草药选[M].云南:云南省思茅地区革命委员会,1971:162.
[5] 徐叔云,卞如镰,陈修.药理实验方法学[M].3版.北京:人民卫生出版社,2012:1203.
[6] 陈清华,莫莉,袁肇凯,等.养心通脉方有效成分部位抗心肌缺血的实验研究[J].中华中医药学刊,2008,26(9):1910-1913.
[7] 明磊,卢丹,刘金平,等.抗心肌缺血中药有效成分研究进展[J].人参研究,2010(3):38-42.
[8] 杨仁洲,周俊,郑启泰,等.一个新奇的单萜甙——黑蒴甙[J].云南植物研究,1983,5(2):215-218.
[9] 杨仁洲,周俊.黑蒴中的单萜甙类化合物[J].云南植物研究,1987,9(1):103-107.
[10] 苏成业,杨彤,曹士宁,等.七种中草药的不同提取物对动物移植性肿瘤的影响[J].遵义医学院学报,1995,5(2):31-33,39.
[11] 李育川,房海灵,王定康,等.云南珍稀民族药黑蒴茎芽组织培养快繁体系研究[J].时珍国医国药,2016,27(11):2746-2749.