嗜酸性氧化亚铁硫杆菌代谢产物的常压化学电离质谱分析
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摘要
本研究以721矿和745矿嗜酸性氧化亚铁硫杆菌为研究对象,采用常压化学电离质谱直接分析其代谢产物,分别考察了顶空采样(Headspace sampling)、界面采样(Interface sampling)和中性解吸采样(Neutral desorption sampling)3种进样方式对电离效果的影响。在优化条件下,常压化学电离质谱对微生物纯菌种和混合菌种的代谢产物均具有良好的分析能力,可根据获得的代谢产物指纹谱图结合主成分分析(PCA)方法和聚类分析(CA)方法区分2个放射性强弱不同区域共4类嗜酸性微生物样品,并对主要胺类、酯类等代谢成分进行串联质谱鉴定,为耐辐射微生物的相关研究提供了一种可借鉴的分析方法。
A method for rapid determination of acidithiobacillus ferrooxidans(At. f)metabolites by ambient corona discharge ionization mass spectrometry was established. Three injection modes were applied to study the effects such as headspace sampling,interface sampling and neutral desorption sampling. Under the optimized experimental conditions,the metabolites,such as small molecule esters and amines were detected by ambient corona discharge ionization mass spectrometry and confirmed using tandem mass spectrometry(MS / MS).Further principal component analysis(PCA)and cluster analysis(CA)of the mass spectrometric results allowed a confident discrimination of different bacterial samples. Ambient corona discharge ionization mass spectrometry has the advantages of no sample pretreatment,convenient operation,high sensitivity and high analytical speed,which will be an attractive method to rapidly identification of microorganisms.
A method for rapid determination of acidithiobacillus ferrooxidans(At. f)metabolites by ambient corona discharge ionization mass spectrometry was established. Three injection modes were applied to study the effects such as headspace sampling,interface sampling and neutral desorption sampling. Under the optimized experimental conditions,the metabolites,such as small molecule esters and amines were detected by ambient corona discharge ionization mass spectrometry and confirmed using tandem mass spectrometry(MS / MS).Further principal component analysis(PCA)and cluster analysis(CA)of the mass spectrometric results allowed a confident discrimination of different bacterial samples. Ambient corona discharge ionization mass spectrometry has the advantages of no sample pretreatment,convenient operation,high sensitivity and high analytical speed,which will be an attractive method to rapidly identification of microorganisms.
引文
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19 LUO Li-Ping,WANG Jiang,ZHANG Wen-Jun,DAI Xi-Mo,FANG Xiao-Wei,ZHANG Qian,LIU Ya-Li,CHEN HuanWen.Chinese J.Anal.Chem.,2013,41(7):1050-1056罗丽萍,王姜,章文军,戴喜末,方小伟,张茜,刘亚丽,陈焕文.分析化学,2013,41(7):1050-1056
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2 SHEN Jun-Jian.Study on Screening and Culturing of Biometallurgical Microorganisms and Their Leaching Process in Low grade Tellurium Ore.Chengdu Univ.Technol.,2013沈俊剑.低品位碲矿浸矿微生物选育及其浸出工艺研究.成都理工大学,2013
3 ZHOU Hong-Bo,MAO Feng,WANG Yu-Guang.Bull.Mineral.,Petrol.Geochem.,2015,34(2):269-276周洪波,毛峰,王玉光.矿物岩石地球化学通报,2015,34(2):269-276
4 Kelly D,Wood A.Int.J.Syst.Evol.Micr.,2000,50(2):511-516
5 Colmer A,Hinkle M.Science,1947,106(2751):253-256
6 Temple K,Colmer A.J.Bacteriol.,1951,62(5):605-611
7 QIU Guan-Zhou.Biohydrometallurgy.Central South University Press,2011邱冠周.生物湿法冶金.中南大学出版社,2011
8 Acevedo F.Electron.J.Biotechn.,2002,5(2):196-199
9 Bosecker K.Fems Microbiol.Rev.,1997,20(3):591-604
10 YANG Xian-Wan,SHEN Qing-Feng,GUO Yu-Xia.Microbial Hydrometallurgy,Metallurgical Industry Press,2003杨显万,沈庆峰,郭玉霞.微生物湿法冶金.冶金工业出版社,2003
11 LIANG Hua-Zheng,ZHANG Xie,RAO Jun,CHEN Huan-Wen.J.Chin.Biotechnol.,2008,28(1):124-133梁华正,张燮,饶军,陈焕文.中国生物工程杂志,2008,28(1):124-133
12 Chingin K,Liang J,Chen H.Rsc.Adv.,2014,4(11):5768-5781
13 Liang J,Hang Y,Chingin K,Hu L,Chen H.Rsc.Adv.,2014,4(48):25326-253269
14 YANG Shui-Ping,HU Bin,LI Jian-Qian,HAN Jing,ZHANG Xie,CHEN Huan-Wen,LIU Qing,LIU Qing-Jun,ZHENG Jian.Chinese J.Anal.Chem.,2009,37(5):691-694杨水平,胡斌,李建强,韩京,张燮,陈焕文,刘清,刘清珺,郑健.分析化学,2009,37(5):691-694
15 CHEN Huan-Wen,LAI Jin-Hu,ZHOU Yu-Feng,HUAN Yan-Fu,LI Jian-Qian,ZHANG Xie,WANG Zhi-Chang,LUO Ming-Biao.Chinese J.Anal.Chem.,2007,35(8):1233-1240陈焕文,赖劲虎,周瑜芬,郇延富,李建强,张燮,王志畅,罗明标.分析化学,2007,35(8):1233-1240
16 JIANG Guo-Fang,LE Zhang-Gao,XIE Zong-Bo.Chinese J.Spectr.Lab.,2004,21(4):782-784姜国芳,乐长高,谢宗波.光谱实验室,2004,21(4):782-784
17 WEI Yi-Ping,YAN Fei-Yan,JIA Bin,DING Jian-Hua,PENG Jin-Hua,CHEN Huan-Wen,XUN Jian-Jun.Chinese J.Exp.Surg.,2011,28(3):422-424魏益平,鄢飞燕,贾滨,丁健桦,彭金华,陈焕文,徐建军.中华实验外科杂志,2011,28(3):422-424
18 WANG Jiang,LI Qian,GU Hai-Wei,GUO Xiao-Tun,YANG Shui-Ping,WANG Zhi-Hao.Chem.J.Chinese Universities,2015,1(6):1067-1073王姜,李倩,顾海巍,郭晓暾,杨水平,王志豪.高等学校化学学报,2015,1(6):1067-1073
19 LUO Li-Ping,WANG Jiang,ZHANG Wen-Jun,DAI Xi-Mo,FANG Xiao-Wei,ZHANG Qian,LIU Ya-Li,CHEN HuanWen.Chinese J.Anal.Chem.,2013,41(7):1050-1056罗丽萍,王姜,章文军,戴喜末,方小伟,张茜,刘亚丽,陈焕文.分析化学,2013,41(7):1050-1056
20 ZHU Zhi-Qiang,YAN Jian-Ping,WANG Yu,CHEN Shun-Zong,CHEN Huan-Wen.Chinese J.Anal.Chem.,2013,41(6):905-910朱志强,闫建平,汪雨,陈瞬宗,陈焕文.分析化学,2013,41(6):905-910