香椿种子无菌苗再生体系的建立
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摘要
为了建立香椿无性系规模化组培快繁技术,以成熟香椿种子在无菌条件下萌发的幼苗茎段为外植体,研究不同基本培养基和不同植物生长调节剂对丛芽诱导、增殖、壮苗及生根的影响。结果表明:最佳消毒处理为20%NaClO消毒20 min,萌发率为80.56%,污染率为27.27%;萌发培养基为WPM或1/2MS+3.0 mg·L~(-1)GA_3;最佳丛芽诱导培养基为MS+1.0 mg·L~(-1)6-BA+0.1 mg·L~(-1)NAA+1.0 mg·L~(-1)GA_3,增殖倍数为4.82;最佳壮苗培养基MS+0.5 mg·L~(-1)6-BA+0.05 mg·L~(-1)IAA+2.0 mg·L~(-1)GA_3;最佳生根培养基MS+2.0 mg·L~(-1)IBA,生根率为78.16%,平均生根条数为4.8。炼苗后,移栽到园土∶草炭土∶珍珠岩体积比为2∶2∶1的基质中,成活率达77.5%。本研究为香椿良种快繁提供了一条新途径,并较其他外植体(叶片、带芽茎段)为起始材料有不受取材条件限制的优点。
In order to establish the tissue culture and rapid propagation system of Toona sinensis. In this study, the seedlings of mature Toona sinensis seed germination under sterile conditions were used as explants to study the influence of different medium and different plant growth regulators on bud induction, hyperplasia, sound seedling and rhizogenesis and establish a complete system of tissue culture and rapid propagation. The results showed that the best disinfection treatment was 20% Na Cl O disinfection for 20 min, the germination rate was 80.56%, the contamination rate was 27.27%.The best germination medium was 1/2 MS or WPM+3.0 mg·L~(-1) GA_3, and the best medium for bud induction was MS+1.0 mg·L~(-1)6-BA+0.1 mg·L~(-1) NAA +1.0 mg·L~(-1) GA_3, the proliferation times was 4.82.The best Seedling medium was MS+0.5 mg·L~(-1)6-BA+0.05 mg·L~(-1) IAA+2.0 mg·L~(-1) GA_3. The best medium for rooting was MS+2.0 mg·L~(-1) IBA, the rooting rate was 78. 16%, and the average number of rooting shoots was 4.8. The survival rate was 77.5% in the medium of 2:2:1 in peat soil: peat soil: perlite.The research supplied a new way for rapid propagation of Toona sinensis.
In order to establish the tissue culture and rapid propagation system of Toona sinensis. In this study, the seedlings of mature Toona sinensis seed germination under sterile conditions were used as explants to study the influence of different medium and different plant growth regulators on bud induction, hyperplasia, sound seedling and rhizogenesis and establish a complete system of tissue culture and rapid propagation. The results showed that the best disinfection treatment was 20% Na Cl O disinfection for 20 min, the germination rate was 80.56%, the contamination rate was 27.27%.The best germination medium was 1/2 MS or WPM+3.0 mg·L~(-1) GA_3, and the best medium for bud induction was MS+1.0 mg·L~(-1)6-BA+0.1 mg·L~(-1) NAA +1.0 mg·L~(-1) GA_3, the proliferation times was 4.82.The best Seedling medium was MS+0.5 mg·L~(-1)6-BA+0.05 mg·L~(-1) IAA+2.0 mg·L~(-1) GA_3. The best medium for rooting was MS+2.0 mg·L~(-1) IBA, the rooting rate was 78. 16%, and the average number of rooting shoots was 4.8. The survival rate was 77.5% in the medium of 2:2:1 in peat soil: peat soil: perlite.The research supplied a new way for rapid propagation of Toona sinensis.
引文
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[21]晏姝,胡德活,韦如萍,等.南洋楹组培快繁技术优化研究[J].中南林业科技大学学报,2017,37(6):65-69.
[22]郭树义,陈静,王翠霞,等.乌腺金丝桃茎尖离体培养基激素配比筛选[J].经济林研究,2017,35(1):68-72.
[23]许丽琼,涂炳坤.香椿叶片组织培养和快繁技术的研究[J].湖北农业科学,2007(1):24-26.
[24]梁明勤,郭群鹏,陈世昌,等.菜用香椿组培快繁技术研究[J].园艺与种苗,2016(3):58-61.
[25]香椿T4品系组培快繁技术研究[D].杨凌:西北农林科技大学,2006.
[2]张荣,校彦赟,朱牧笛,等.滇中地区香椿萌芽特性和设施内采摘技术研究[J].西南林业大学学报,2016,36(3):60-65.
[3]蒋时姣,钟宇,张帆,等.柳杉种子无菌苗组培快繁体系的建立[J].植物生理学报,2014,50(7):1039-1044.
[4]张晓申,邢廷茂,王慧瑜,等.香椿组织培养与快速繁殖技术的研究[J].江西农业学报,2011,23(10):58-59,62.
[5]许丽琼,涂炳坤.香椿茎段组织培养和再生技术研究[J].华中农业大学学报,2007(5):697-700.
[6]吴玲利,柯镔峰,龚春,等.白木通组织培养及快速繁殖[J].植物生理学报,2015,51(6):903-908.
[7]江涛,李晨曦,陈磊,等.毛竹组织培养中成熟种子消毒方法[J].江苏农业科学,2017,45(4):103-106.
[8]李泽,谭晓风,张琳,等.油桐种胚再生体系的建立[J].经济林研究,2012,30(4):119-122.
[9]吴秀燕,张鸽香.美国流苏离体胚的组织培养与快速繁殖[J].植物生理学报,2017,53(2):227-233.
[10]吴俊长,李萌,曹福祥,等.珙桐种子快速繁殖技术的研究[J].中南林业科技大学学报,2016,36(2):66-70,95.
[11]Rehana S,Alam MS,Islam KS,Samad MA.Influence of growth regulators on shoot proliferation and plantlet production from shoot tips of banana.Prog Agric,2013,20(1-2):9-16.
[12]谈静,张英杰,郭文姣,等.外源激素对大花型红花蝴蝶兰组培增殖、促壮及生根的影响[J].分子植物育种,2018,16(3):943-947.
[13]茅汝佳,高燕.巴迪亚寿的组织培养及育苗技术[J].浙江农业科学,2018,59(3):477-481.
[14]吴秀华,张艳玲,周月,等.‘海沃德’猕猴桃叶片高频直接再生体系的建立[J].植物生理学报,2013,49(8):759~763.
[15]吴雅文,李枝林,白天,等.迷人杜鹃组培快繁技术的研究[J].种子,2015,34(3):112-116.
[16]孙英坤,胡绍庆,庞基良,等.珍稀濒危物种堇叶紫金牛高效快繁体系的建立[J].植物学报,2017,52(6):764-773.
[17]李泽,谭晓风,袁军,等.油茶良种‘华硕’的组织培养及高效生根[J].植物生理学报,2014,50(11):1721-1726.
[18]吴玲利.白木通组织培养再生体系的建立[D].长沙:中南林业科技大学,2016.
[19]田奥磊,高俊杰,李丹丹,等.武夷岩茶大红袍的组织培养与快速繁殖[J].植物生理学报,2017,53(4):619-624.
[20]陈轲.红椿组织培养技术研究[D].长沙:中南林业科技大学,2016.
[21]晏姝,胡德活,韦如萍,等.南洋楹组培快繁技术优化研究[J].中南林业科技大学学报,2017,37(6):65-69.
[22]郭树义,陈静,王翠霞,等.乌腺金丝桃茎尖离体培养基激素配比筛选[J].经济林研究,2017,35(1):68-72.
[23]许丽琼,涂炳坤.香椿叶片组织培养和快繁技术的研究[J].湖北农业科学,2007(1):24-26.
[24]梁明勤,郭群鹏,陈世昌,等.菜用香椿组培快繁技术研究[J].园艺与种苗,2016(3):58-61.
[25]香椿T4品系组培快繁技术研究[D].杨凌:西北农林科技大学,2006.