山银花、金银花中绿原酸和总黄酮含量及抗氧化活性测定

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英文篇名：Determination of chlorogenic acid, total flavones, and anti-oxidant activity of Flos Lonicerae Japonicae and Flos Lonicerae 作者：肖作为 ; 谢梦洲 ; 甘龙 ; 方茗妍 ; 周新源 ; 周一苗 ; 贺炜 ; 陈林 ; 黄建华 英文作者：XIAO Zuo-wei;XIE Meng-zhou;GAN Long;FANG Ming-yan;ZHOU Xin-yuan;ZHOU Yi-miao;HE Wei;CHEN Lin;HUANG Jian-hua;School of Pharmacy, Hunan University of Chinese Medicine;Resgreen Bioengineering Group Co., Ltd.;Hunan Engineering Research Center of Drug and Food Homology Functional Food;Institution of Chinese Medicine; 关键词：山银花 ; 金银花 ; 抗氧化活性 ; 绿原酸 ; 总黄酮 英文关键词：Flos Lonicerae Japonicae;;Flos Lonicerae;;anti-oxidant activity;;chlorogenic acid;;total flavonoids 中文刊名：ZCYO 英文刊名：Chinese Traditional and Herbal Drugs 机构：湖南中医药大学药学院;湖南省药食同源功能性食品工程技术中心;绿之韵生物工程集团有限公司;湖南省中医药研究院; 出版日期：2019-01-12 出版单位：中草药 年：2019 期：v.50;No.636 基金：国家自然科学基金资助项目(81603400);; 长沙市杰出创新青年培养计划(kq1802017);; 湖南省中医药管理局项目(201702);; 湖南省“中药学”重点学科资助项目(湘教通[2011] 76号);; 湖南大学生创新课题(0110-0901002016,1021-0001017096);; 国家重点学科中医诊断学开放基金资助项目 语种：中文; 页：ZCYO201901031 页数：7 CN：01 ISSN：12-1108/R 分类号：213-219

摘要

目的对3个不同产地山银花和金银花中绿原酸、总黄酮含量及抗氧化活性进行分析,并比较之间的差异。方法对绿原酸提取条件进行优化,最佳提取条件为65℃下60%乙醇溶液按料液比1∶20超声提取30 min;总黄酮最佳提取条件为65℃下用60%甲醇溶液按料液比1∶10超声提取30 min。分别采用高效液相色谱法和紫外分光光度法对样品的绿原酸和总黄酮进行含量测定,并用DPPH自由基清除率来检测山银花、金银花的抗氧化活性。结果建立了良好的绿原酸和总黄酮含量分析方法,绿原酸在0.119～1.190 mg/m L内线性关系良好,r2为0.999 2(n=6);总黄酮在8.48～50.88μg/m L内线性关系良好,r2为0.999 5(n=6)。湖南产地山银花绿原酸量、总黄酮量及自由基清除率分别为3.99%、13.43%、62.41%。重庆产地山银花绿原酸量、总黄酮量及自由基清除率分别为3.29%、10.08%、51.48%。广西产地金银花绿原酸量、总黄酮量、自由基清除率分别为2.55%、7.10%、39.51%。结论提出了结合化学成分分析和抗氧化性活性来比较不同产地山银花和金银花之间区别的分析方法,从"谱-效"结合思路,为山银花和金银花质量控制及鉴别提供一种新的模式。
        Objective To determine the content of chlorogenic acid, total flavones, and anti-oxidant activity in Flos Lonicerae japonicae and Flos Lonicerae obtained from three different origins and compare their differences. Methods The optimized extraction conditions of chlorogenic acid were ultrasonic extraction 30 min in 65 ℃ with ethanol: water(60︰40) and solid-liquid ratio(1︰20). The optimized extraction conditions of total flavonoids were ultrasonic extraction 30 min at 65 ℃ with 60% methanol solution, solid-liquid ratio(1︰10). HPLC and UV spectrophotometry were used to determine the content of chlorogenic acid and total flavonoids in the samples, and then estimation of anti-oxidative activity of Flos Lonicerae japonicae and Flos Lonicerae by DPPH radical scavenging capacity method. Results A method for the analysis of chlorogenic acid and total flavonoids was established, which have a good linear relationship of chlorogenic acid in 0.119—1.190 mg/mL and R2 was 0.999 2(n = 6); A good linear relationship between 0.008 and 0.050 mg/mL and r2 was 0.999 5(n = 6) for analysis of the total flavonoids. The average content of chlorogenic acid, total flavonoids, and free radical scavenging rate of Flos Lonicerae from Hunan Province was 3.99%, 13.43%, and 62.41%, respectively. The average content of chlorogenic acid, total flavonoids and free radical scavenging rate of Flos Lonicerae from Chongqing were 3.29%, 10.08%, and 51.48% respectively. The average content of chlorogenic acid, total flavonoids, and free radical scavenging rate of Flos Lonicerae japonicae from Guangxi Province were 2.55%, 7.10%, and 39.51%, respectively. Conclusion This study proposed an analytical method combining the chemical composition analysis and anti-oxidant activity to compare the differences between the different producing areas of Flos Lonicerae japonicae and Flos Lonicerae. Combining the "spectrum-effect", it provides a new model for the quality control and identification of two plants.

引文

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