结直肠癌组织中Ki-67和pAKT的表达及其临床意义
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摘要
[目的]探讨结直肠癌组织中Ki-67和pAKT的表达及其临床意义,为临床诊治结直肠癌提供依据。[方法]选取结直肠癌患者80例,患者均经病理确诊为结直肠癌。应用免疫组织化学SP法检测80例结直肠癌组织(结直肠癌组)、80例结直肠癌癌旁组织(癌旁组)、20例正常大肠组织(正常组)中Ki-67和pAKT的表达阳性率。分析结直肠癌患者癌组织中Ki-67和pAKT表达与结直肠癌转移、分期及预后的关系。采用Spearman相关分析法分析Ki-67及pAKT表达的相关性。[结果]结直肠癌组中Ki-67和pAKT的表达阳性率明显高于癌旁组和正常组的阳性率(P=0.000,0.000,0.012)(P=0.000,0.011,0.024)。有淋巴结转移的患者癌变组织中Ki-67和pAKT表达阳性率明显高于无淋巴结转移患者(P=0.013,P=0.009);Ⅰ+Ⅱ期患者癌组织中Ki-67和pAKT表达阳性率明显低于Ⅲ+Ⅳ分期(P=0.003,P=0.002);Ki-67与pAKT表达呈正相关关系(r=0.520,P=0.020);Ki-67阴性表达患者总生存率与Ki-67表达(+)、(++)及(+++)的患者总生存率比较差异有统计学意义(P=0.021,0.022,0.000);pAKT阴性表达患者总生存率与pAKT表达(+)、(++)及(+++)的患者总生存率比较差异有统计学意义(P=0.011,0.012,0.001)。[结论]Ki-67和pAKT异常表达与结直肠癌的发生发展密切相关,其表达上调参与肿瘤形成、发展、侵袭和转移,可作为判断结肠癌生物学行为的临床参考指标。
[Objective]To investigate the expressions and clinical significance of Ki-67 and serine/threonine protein kinase(pAKT)in colorectal cancer tissue,and to provide basis for diagnosis and treatment of colorectal cancer.[Methods]A total of 80 colorectal cancer patients were selected,and the patients were diagnosed as colorectal cancer by pathology.The expression rate of Ki-67 and pAKT in colorectal cancer tissues(colorectal cancer group,80cases),para-carcinoma tissues(para-carcinoma group,80cases)and normal colorectal tissues(normal group,20cases)were detected by immunohistochemical SP method.The relationship between the expressions of Ki-67 and pAKT and colorectal cancer metastasis,different stages and progosis were analyzed.The correlation between the expressions of Ki-67 and pAKT were analysis by Spearman.[Results]The psitive expression rates of pAKT and Ki-67 in colorectal cancer tissue were obviously higher than those in adjacent tissues and normal mucosal tissues(P =0.000,0.000,0.012)(P =0.000,0.011,0.024).The positive exprssion rates of pAKT and Ki-67 expression in patients with lymph node metastasis were significantly higher than those without lymph node metastasis(X~2=7.484,P =0.013;X~2=9.953,P=0.009).The positive rate of Ki-67 and pAKT in cancer tissues of patients withⅠ+Ⅱ were significantly less than those with Ⅲ+Ⅳ(X~2=12.067,P=0.003;X~2=11.542,P=0.002).Ki-67 was positively correlated with pAKT expression(r=0.520,P=0.020)(r=0.520,P=0.020);The difference in overall survival rate between patients with ki-67 negative expression and those with ki-67expression(+),(++)and(+++)was statistically significant(P=0.021,0.022,0.000).The difference in total survival rate between pAKT negative expression patients and pAKT expression(+),(++)and(+++)patients was statistically significant(P=0.011,0.012,0.001).[Conclusion]The abnormal expressions of pAKT and Ki-67 is closely related to the occurrence and development of colorectal cancer,and its up-regulated expression is involved in tumor formation,development,invasion and metastasis,and can be used as a clinical reference index for judging the biological behavior of colon cancer.
[Objective]To investigate the expressions and clinical significance of Ki-67 and serine/threonine protein kinase(pAKT)in colorectal cancer tissue,and to provide basis for diagnosis and treatment of colorectal cancer.[Methods]A total of 80 colorectal cancer patients were selected,and the patients were diagnosed as colorectal cancer by pathology.The expression rate of Ki-67 and pAKT in colorectal cancer tissues(colorectal cancer group,80cases),para-carcinoma tissues(para-carcinoma group,80cases)and normal colorectal tissues(normal group,20cases)were detected by immunohistochemical SP method.The relationship between the expressions of Ki-67 and pAKT and colorectal cancer metastasis,different stages and progosis were analyzed.The correlation between the expressions of Ki-67 and pAKT were analysis by Spearman.[Results]The psitive expression rates of pAKT and Ki-67 in colorectal cancer tissue were obviously higher than those in adjacent tissues and normal mucosal tissues(P =0.000,0.000,0.012)(P =0.000,0.011,0.024).The positive exprssion rates of pAKT and Ki-67 expression in patients with lymph node metastasis were significantly higher than those without lymph node metastasis(X~2=7.484,P =0.013;X~2=9.953,P=0.009).The positive rate of Ki-67 and pAKT in cancer tissues of patients withⅠ+Ⅱ were significantly less than those with Ⅲ+Ⅳ(X~2=12.067,P=0.003;X~2=11.542,P=0.002).Ki-67 was positively correlated with pAKT expression(r=0.520,P=0.020)(r=0.520,P=0.020);The difference in overall survival rate between patients with ki-67 negative expression and those with ki-67expression(+),(++)and(+++)was statistically significant(P=0.021,0.022,0.000).The difference in total survival rate between pAKT negative expression patients and pAKT expression(+),(++)and(+++)patients was statistically significant(P=0.011,0.012,0.001).[Conclusion]The abnormal expressions of pAKT and Ki-67 is closely related to the occurrence and development of colorectal cancer,and its up-regulated expression is involved in tumor formation,development,invasion and metastasis,and can be used as a clinical reference index for judging the biological behavior of colon cancer.
引文
[1]胡兵,安红梅,郑佳露,等.藤龙补中汤对大肠癌RKO细胞转移相关基因表达影响[J].中国中西医结合消化杂志,2018,26(5):416-419.
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[3] Alexopoulou AN,Ho-Yen CM,Papalazarou V,et al.Tumour associated endothelial-FAK correlated with molecular sub-type and prognostic factors in invasive breast cancer[J].BMC Cancer,2014,14(3):237.
[4]李建,周怀君.PI3K/Akt信号通路在肿瘤研究中的进展[J].东南大学学报(医学版),2014,33(3):392-395.
[5]刁存启,毕经旺,王宝成.Akt信号分子和结直肠癌的关系[J].中国肿瘤生物治疗杂志,2014,21(5):584-588.
[6]邓海霞,陈更新,周奥强,等.养正散结汤对裸鼠胃癌移植瘤生长及let-7a/c-Myc表达的影响[J].中国中西医结合消化杂志,2017,25(10):738-741.
[7]徐璐,石晶,张凌云,等.FAK及pAKT在乳腺癌中的表达及预后分析[J].中国医科大学学报,2015,44(8):673-678.
[8]郝鑫,张刚,李中.乳腺浸润性导管癌组织中p-AKT的表达[J].中国老年学杂志,2017,37(16):3938-3940.
[9] Machado VA,Peixoto D,Queiroz MJ,et al.Antiangiogenic 1-Aryl-3-[3-(thieno[3,2-b]pyridin-7-ylthio)phenyl]ureas Inhibit MCF-7and MDA-MB-231 human breast cancer cell lines through PI3 K/Akt and MAPK/Erk pathways[J].J Cell Biochem,2016,117(12):2791-2799.
[10]杨大运,邢亚威,张月花,等.老年非小细胞肺癌中Raf激酶抑制蛋白与p-AKT的表达及意义[J].中国老年学杂志,2016,36(3):644-645.
[11]Avan A,Narayan R,Giovannetti E,et al.Role of Akt signaling in resistance to DNA-targeted therapy[J].World J Clin Oncol,2016,7(5):352-369.
[12]Majumder M,Landman E,Liu L,et al.pAKT Elevates Oncogenic mi R-526bin Breast Cancer by EP4Activation[J].Mol Cancer Res,2015,13(6):1022-1033.
[13]史汉蒙,司君利,崔伟丽,等.大肠癌组织中肿瘤相关巨噬细胞计数与MMP-2、Ki-67表达的相关性研究[J].中国现代普通外科进展,2015,18(2):94-98.
[14]李伟,张庚,王海伦,等.细胞周期蛋白E、细胞核增殖抗原、基质金属蛋白酶-9在大肠癌组织的表达及临床意义[J].中华实验外科杂志,2017,34(1):27-30.
[15]樊子双,孟杰,贝雪艳,等.Ki-67蛋白在大肠癌中的研究进展[J].河北医学,2016,22(7):1225-1226.
[16]任丽文,薛玲珑,贾静.Ki-67、survivin在伴鸡皮样改变大肠腺瘤中表达的临床意义[J].中华临床医师杂志(电子版),2017,11(1):17-21.
[17]章亿刚,阮利霞.Ki-67和pAKT在结肠癌组织中的表达[J].临床荟萃,2014,29(2):182-185.
[18]蓝显明,曾彬,唐淑妮.结直肠癌淋巴结转移与PTEN、pAKT表达的相关性[J].实用癌症杂志,2015,30(9):1296-1298.
[19]黄芳,钱国伟.刘俊杰,等.ALDHl、EpCAM及Ki67mRNA在结直肠癌患者循外肿瘤细胞中的表达及意义[J].中国肿瘤外科杂志,2013,5(4):235-238.
[20]李,陈志英.NF-κB及pAKT在结直肠癌中表达相关性[J].四川医学,2013,34(8):1102-1104.
[2] Annika G,Asting A,Farivar BM,et al.EGF receptor and COX-1/COX-2enzyme proteins as related to corresponding mRNAs in human per-operative biopsies of colorectal cancer[J].BMC Cancer,2015,13(4):511-512.
[3] Alexopoulou AN,Ho-Yen CM,Papalazarou V,et al.Tumour associated endothelial-FAK correlated with molecular sub-type and prognostic factors in invasive breast cancer[J].BMC Cancer,2014,14(3):237.
[4]李建,周怀君.PI3K/Akt信号通路在肿瘤研究中的进展[J].东南大学学报(医学版),2014,33(3):392-395.
[5]刁存启,毕经旺,王宝成.Akt信号分子和结直肠癌的关系[J].中国肿瘤生物治疗杂志,2014,21(5):584-588.
[6]邓海霞,陈更新,周奥强,等.养正散结汤对裸鼠胃癌移植瘤生长及let-7a/c-Myc表达的影响[J].中国中西医结合消化杂志,2017,25(10):738-741.
[7]徐璐,石晶,张凌云,等.FAK及pAKT在乳腺癌中的表达及预后分析[J].中国医科大学学报,2015,44(8):673-678.
[8]郝鑫,张刚,李中.乳腺浸润性导管癌组织中p-AKT的表达[J].中国老年学杂志,2017,37(16):3938-3940.
[9] Machado VA,Peixoto D,Queiroz MJ,et al.Antiangiogenic 1-Aryl-3-[3-(thieno[3,2-b]pyridin-7-ylthio)phenyl]ureas Inhibit MCF-7and MDA-MB-231 human breast cancer cell lines through PI3 K/Akt and MAPK/Erk pathways[J].J Cell Biochem,2016,117(12):2791-2799.
[10]杨大运,邢亚威,张月花,等.老年非小细胞肺癌中Raf激酶抑制蛋白与p-AKT的表达及意义[J].中国老年学杂志,2016,36(3):644-645.
[11]Avan A,Narayan R,Giovannetti E,et al.Role of Akt signaling in resistance to DNA-targeted therapy[J].World J Clin Oncol,2016,7(5):352-369.
[12]Majumder M,Landman E,Liu L,et al.pAKT Elevates Oncogenic mi R-526bin Breast Cancer by EP4Activation[J].Mol Cancer Res,2015,13(6):1022-1033.
[13]史汉蒙,司君利,崔伟丽,等.大肠癌组织中肿瘤相关巨噬细胞计数与MMP-2、Ki-67表达的相关性研究[J].中国现代普通外科进展,2015,18(2):94-98.
[14]李伟,张庚,王海伦,等.细胞周期蛋白E、细胞核增殖抗原、基质金属蛋白酶-9在大肠癌组织的表达及临床意义[J].中华实验外科杂志,2017,34(1):27-30.
[15]樊子双,孟杰,贝雪艳,等.Ki-67蛋白在大肠癌中的研究进展[J].河北医学,2016,22(7):1225-1226.
[16]任丽文,薛玲珑,贾静.Ki-67、survivin在伴鸡皮样改变大肠腺瘤中表达的临床意义[J].中华临床医师杂志(电子版),2017,11(1):17-21.
[17]章亿刚,阮利霞.Ki-67和pAKT在结肠癌组织中的表达[J].临床荟萃,2014,29(2):182-185.
[18]蓝显明,曾彬,唐淑妮.结直肠癌淋巴结转移与PTEN、pAKT表达的相关性[J].实用癌症杂志,2015,30(9):1296-1298.
[19]黄芳,钱国伟.刘俊杰,等.ALDHl、EpCAM及Ki67mRNA在结直肠癌患者循外肿瘤细胞中的表达及意义[J].中国肿瘤外科杂志,2013,5(4):235-238.
[20]李,陈志英.NF-κB及pAKT在结直肠癌中表达相关性[J].四川医学,2013,34(8):1102-1104.