番木瓜育种研究进展与展望
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摘要
番木瓜是一种药食兼用的植物,长期食用可以增强人体免疫力,达到改善体质的目的。因此提高番木瓜的产量、品质和药用价值变得十分重要。育种技术是改善番木瓜相应指标的重要途径。本综述通过介绍番木瓜传统育种、多倍体育种、辐射诱变育种、细胞工程育种、分子标记辅助育种和基因工程育种等育种手段,并根据目前育种学发展的趋势,指明今后番木瓜的育种研究方向。目前分子模块设计育种是今后番木瓜育种研究的一个新的有效途径。因此本综述通过对利用自然变异的抗病性品种、运用现代生物技术等手段筛选和培育出新的番木瓜品种的概述,以期为对番木瓜育种技术发展情况进行了较为全面的总结,并对番木瓜育种发展进行提供理论参考。
Papaya is a kind of medicinal and edible plant. Long-term consumption can enhance human immunity and achieve the goal of improving physical fitness. Therefore, it is very important to increase the yield, quality and medicinal value of papaya. Breeding technology is an important way to improve the corresponding indicators of papaya. In this review, the methods of traditional breeding, polyploid breeding, radiation mutation breeding, cell engineering breeding, molecular marker assisted breeding and genetic engineering breeding of papaya were introduced, and the future research direction of papaya breeding was pointed out according to the current trend of breeding. At present, molecular module design breeding is a new effective way for papaya breeding in the future.Therefore, this review summarized and developed new papaya varieties by using disease-resistant varieties with natural variation and using modern biotechnology, hoping to provide a comprehensive summary of the development of papaya breeding technology and theoretical reference for papaya breeding development.
Papaya is a kind of medicinal and edible plant. Long-term consumption can enhance human immunity and achieve the goal of improving physical fitness. Therefore, it is very important to increase the yield, quality and medicinal value of papaya. Breeding technology is an important way to improve the corresponding indicators of papaya. In this review, the methods of traditional breeding, polyploid breeding, radiation mutation breeding, cell engineering breeding, molecular marker assisted breeding and genetic engineering breeding of papaya were introduced, and the future research direction of papaya breeding was pointed out according to the current trend of breeding. At present, molecular module design breeding is a new effective way for papaya breeding in the future.Therefore, this review summarized and developed new papaya varieties by using disease-resistant varieties with natural variation and using modern biotechnology, hoping to provide a comprehensive summary of the development of papaya breeding technology and theoretical reference for papaya breeding development.
引文
Cai Y.Q.,and Lai Z.X.,2003,Advances in research on biotechnology of Carica papaya L.,Jiangxi Nongye Daxue Xuebao(Acta Agriculturae Universitatis Jiangxiensis),25(3):429-434(蔡英卿,赖钟雄,2003,番木瓜生物技术研究进展,江西农业大学学报,25(3):429-434)
Chen C.B.,Li X.Y.,and Zhou P.,2006,Review on breeding and selection of disease resistant lines of papaya and their tissue culture,Redai Nongye Kexue(Chinese Journal of Tropical Agriculture),28(6):47-52(陈春宝,黎小瑛,周鹏,2006,番木瓜抗病育种及其组培苗生产概述,热带农业科学,28(6):47-52)
Chen M.H.,Wang P.J.,and Maeda E.,1987,Somatic embryogenesis and plant regeneration in Carica papaya L.tissue culture derived form root explants,Plant Cell Reports,6(5)348-351
Chen Z.H.,2001,studies on the biochemical and molecular marker for papaya(Carica papya L.)sexuality,Thesis for MS.,Fujian Agricultural and forestry University,Supervisor Chen X.J.,pp.16-25(陈中海,2001,番木瓜性别的生化标记与分子标记研究,硕士学位论文,福建农业大学,导师陈晓静,pp.16-25)
DeOliveira J.G.,and Vitória A.P.,2011,Papaya:Nutritional and pharmacological characterization and quality loss duetophysiological disorders an overview,Food Research International,44(5):1306-1313
Fitch M.M.,Manshardt R.M.,Gonsalves D.,Slightom J.L.,and Sanford J.C.,1990,Stable transformation of papaya via microprojectile bombardment,Plant Cell Rep.,9(4):189-194
Gao H.,Yi J.Y.,Bi J.F.,Liu X.,Deng F.M.,and Wu X.Y.,2015Drying characteristics and quality of Carica papaya Linn under Med-and Short-wave infrared radiation and hot air drying,Xiandai Shipin Keji(Modern Food Science and Technology),31(2):190-195,172(高鹤,易建勇,毕金峰,刘璇邓放明,吴昕烨,2015,中短波红外和热风干燥对番木瓜干燥特性及品质的比较,现代食品科技,31(2):190-195172)
Huang J.C.,and Xiao Y.,2003,Biological effects of irradiation on Carica papaya seeds combined with giberrellin,Xinan Nongye Daxue Xuebao(Journal of Southwest Agricultural University),25(3):203-206(黄建昌,肖艳,2003,番木瓜种子的辐射生物学效应研究,西南农业大学学报,25(3)203-206)
Huang J.C.,Xiao Y.,Liang G.S.,Zhao C.X.,and Li H.B.,2004A preliminary study of the breeding of a prsv-resistan strain of papaya(Carica papaya L.),Xinan Nongye Daxue Xuebao(Journal of Southwest University),26(6):25-28(黄建昌,肖艳,梁关生,赵春香,李宏彬,2004,抗环斑型花叶病毒病番木瓜新品种选育研究初报,西南农业大学,26(6):25-28)
Huang J.C.,Ye K.N.,and Li B.J.,1995,Field experiment of hybrid papaya artificial seeds,Zhongshan Daxue Xuebao(Acta Scientiarum Naturalium Universitatis Sunyatseni),34(3):120-121(黄俊潮,叶克难,李宝健,1995,杂种番木瓜人工种子田间试验,中山大学学报(自然科学版),34(3):120-121)
Jia R.,Zhao H.,Huang J.,Kong H.,Zhang Y.,Guo J.,Huang Q.,Guo Y.,Wei Q.,Zuo J.,Zhu Y.J.,Peng M.,and Guo A.,2017,Publisher Correction:use of RNAi technology to develop a PRSV-resistant transgenic papaya,Sci.Rep.,7(1):16390
Kim M.S.,Moore P.H.,Zee F.,Fitch M.M.,Steiger D.L.,Manshardt R.M.,Paull R.E.,Drew R.A.,Sekioka T.,and Ming R.,2002,Genetic diversity of Carica papaya as revealed by AFLP markers,Genome,45(3):503-512
Kumar A.,Basavaraj Y.B.,Kumar P.,Mishra P.,and Singh S.K.,2018,Development of transgene construct using coat protein gene of papaya ringspot virus and its validation,Progressive Agriculture,18(1):92-97
Lin G.X.,Zhou C.Q.,You K.Z.,and Li W.H.,2005,Current research progress and prospect of papaya in China,Guongdong Nongye Kexue(Guangdong Agricultural Sciences),(4):22-24(林冠雄,周常清,游恺哲,李卫红,2005,我国番木瓜育种研究进展与展望,广东农业科学,(4):22-24)
Ming R.,Hou S.,Feng Y.,Yu Q.,Dionne-Laporte A.,Saw J.H.,Senin P.,Wang W.,Ly B.V.,Lewis K.L.,Salzberg S.L.,Feng L.,Jones M.R.,Skelton R.L.,Murray J.E.,Chen C.,Qian W.,Shen J.,Du P.,Eustice M.,Tong E.,Tang H.,Lyons E.,Paull R.E.,Michael T.P.,Wall K.,Rice D.W.,Albert H.,Wang M.L.,Zhu Y.J.,Schatz M.,Nagarajan N.,A-cob R.A.,Guan P.,Blas A.,Wai C.M.,Ackerman C.M.,Ren Y.,Liu C.,Wang J.,Wang J.,Na J.K.,Shakirov E.V.,Haas B.,Thimmapuram J.,Nelson D.,Wang X.,Bowers J.E.,Gschwend A.R.,Delcher A.L.,Singh R.,Suzuki J.Y.,Tripathi S.,Neupane K.,Wei H.,Irikura B.,Paidi M.,Jiang N.,Zhang W.,Presting G.,Windsor A.,Navajas-Pérez R.,Torres M.J.,Feltus F.A.,Porter B.,Li Y.,Burroughs A.M.,Luo M.C.,Liu L.,Christopher D.A.,Mount S.M.,Moore P.H.,Sugimura T.,Jiang J.,Schuler M.A.,Friedman V.,Mitchell-Olds T.,Shippen D.E.,dePamphilis C.W.,Palmer J.D.,Freeling M.,Paterson A.H.,Gonsalves D.,Wang L.,and Alam M.,2008,The draft genome of the transgenic tropical fruit tree papaya(Carica papaya Linnaeus),Nature,452(7190):991-996
Qu Y.,Li W.J.,Zhou L.B.,Wang Z.Z.,Dong X.C.,Yu L.X.,Liu Q.F.,and He J.Y.,2007,Research and application of mutagenic effects in plants irradiated by heavy ion beams,Yuanzihe Wuli Pinlun(Nuclear Physics Review),24(4):294-298(曲颖,李文建,周利斌,王转子,董喜存,余丽霞,刘青芳,何金玉,2007,重离子辐射植物的诱变效应研究及应用,原子核物理评论,24(4):294-298)
Rivera-Pastrana D.M.,Yahia E.M.,and González-Aguilar G.A.,2010,Phenolicand carotenoid profiles of papaya fruit(Carica papaya L.)and their contents under low temperature storage,J.Sci.Food Agric.,90(14):2358-2365
Schweiggert R.M.,Mezger D.,Schimpf F.,Steingass C.B.,and Carle R.,2012,Influence of chromoplast morphology on carotenoid bioaccessibility of carrot,mango,papaya,and tomato,Food Chemistry,135(4):2736-2742
Sheen T.F.,Wang H.L.,and Wang D.N.,1998,Control of papaya ringspot virus by cross protection and cultivation techniques Engei Gakkai Zasshi,67(6):1232-1235
Tripathi S.,Suzuki J.Y.,CarrJ B.J.,McQuate G.T.,Ferreira S.A.Manshardt R.M.,Pitz K.Y.,Wall M.M.,and Gonsalves D.2011,Nutritional composition of rainbow papaya,the firs commercialized transgenic fruit crop,Journal of Food Composition and Analysis,24(2):140-147
Urasaki N.,Tokumoto M.,Tarora K.,Ban Y.,Kayano T.,Tanaka H.,Oku H.,Chinen I.,and Terauchi R.,2002,A male and hermaphrodite specific RAPA marker for papaya(Carica papaya L.),Theor.Appl.Genet.,104(2-3):281-285
Wang F.,Cai S.K.,and Tang Y.F.,2008,Advances in studies on variety breeding and rapid propagation technique of tissue cultured seedings in Carica papaya L.,Guangdong Nongye Kexue(Guangdong Agricultural Sciences),(S):117-121(王芳,蔡时可,汤亚飞,2008,番木瓜品种选育及组培快繁技术研究进展,广东农业科学,(S):117-121)
Wang W.X.,Li C.Y.,Xiang S.Q.,Guo Q.G.,Lin X.L.,and Liang G.L.,2008,Polyploid induced from Carica papaya and morphological characteristics compared between tetraploids and diploids,Guoshu Xuebao(Journal of Fruit Science),25(1):115-118(汪卫星,李春艳,向素琼,郭启高,李晓林,梁国鲁,2008,番木瓜四倍体的诱导及形态学分析,果树学报25(1):115-118)
Xue Y.B.,Zhong K.,Han B.,Gui J.F.,Wang T.,Fu X.D.,He ZH.,Chu C.C.,Tian Z.X.,Chen Z.K.,and Lin S.Y.,2015New chapter of designer breeding in china:update on strategic program of molecular module-based designer breeding systems,Zhongguo Kexueyuan Yuankan(Proceedings of the Chinese Academy of Sciences),30(3):393-402,282(薛勇彪,种康,韩斌,桂建芳,王台,傅向东,何祖华,储成才,田志喜,程祝宽,林少扬,2015,开启中国设计育种新篇章-“分子模块设计育种创新体系”战略性先导科技专项进展,中国科学院院刊,30(3):393-402,282)
Ye K.N.,Huang J.C.,and Li B.J.,1993,Study on production of artificial seeds of hybrid papaya,Zhiwu Xuebao(Acta Botanica Sinica),35(S):83-87(叶克难,黄俊潮,李宝健,1993,杂种一代番木瓜人工种子制作的研究,植物学报,35(S):83-87)
Ye K.N.,Ma L.,and Li B.J.,1991,Somatic embryogenesis and plant regeneration from papaya suspension culture,Zhiwu Xuebao(Acta Botanica Sinica),33(7):565-568(叶克难,马蕾,李宝健,1991,番木瓜悬浮培养的体胚发生与植株再生,植物学报,33(7):565-568)
Yie S.T.,and Liaw S.I.,1977,Plant regeneration from shoottips and callus of papaya,In Vitro,13(9):564-568
Yu M.,Ye C.M.,and Li B.J.,2001,A study of transforming PRVCP gene into Carica papaya L.genome,Foshan Kexue Jishu Xueyuan Xuebao(Journal of Foshan University),19(1):59-61(于湄,叶长明,李宝健,2001,PRV外壳蛋白转化番木瓜的研究,佛山科学技术学院学报,19(1):59-61)
Zhou G.H.,Li H.P.,Zhang S.G.,and Fan H.Z.,2001b,RAPDmarkers for hermaphrodite gene in papaya,Redai Yaredai Zhiwu Xuebao(Journal of Tropical and Subtropical Botany),9(3):190-193(周国辉,李华平,张曙光,范怀忠2001b,番木瓜两性基因的RAPD标记,热带亚热带植物学报,9(3):190-193)
Zhou G.H.,Li H.P.,Zhang S.G.,Rao X.Q.,Wang Z.Z.,and Fan H.Z.,2001a,Heredity and RAPD marker of papaya mutant resistance to prsv,Zhiwu Bingli Xuebao(Journal of Plant Pathology),31(2):157-163(周国辉,李华平,张曙光,饶雪琴,王振中,范怀忠,2001a,番木瓜抗环斑病毒突变体抗性遗传及RAPD标记,植物病理学报,31(2):157-163)
Zhou P.,Shen W.T.,Yan P.,and Li X.Y.,2010,Key problemes and solve measures of carica papaya production in China,Redai Shengwu Xuebao(Journal of Tropical Biology),11(3):257-260,264(周鹏,沈文涛,言普,黎小瑛,2010,我国番木瓜产业发展的关键问题及对策,热带生物学报,11(3):257-260,264)
Zhuo R.Y.,Chen Y.T.,and Lu Z.Q.,2002,Advances in MAStechnique,Zhejiang Linye Keji(Journal of Zhejiang Forestry Science and Technology),22(3):7-13(卓仁英,陈益泰,陆志群,2002,分子标记辅助选择育种技术研究动态,浙江林业科技,22(3):7-13)
Zou Y.X.,and Guo H.S.,1992,High frequency of somatic embryogenesis and plant transference to plantlets from Carica papaya L.,Zhongshan Daxue Xuebao(Acta Scientiarum Naturalium Universitatis Sunyatseni),31(3):60-65(邹韵霞,郭惠珊,1992,番木瓜胚珠高频率体胚发生和植株再生,中山大学学报(自然科学版),31(3):60-65)
Chen C.B.,Li X.Y.,and Zhou P.,2006,Review on breeding and selection of disease resistant lines of papaya and their tissue culture,Redai Nongye Kexue(Chinese Journal of Tropical Agriculture),28(6):47-52(陈春宝,黎小瑛,周鹏,2006,番木瓜抗病育种及其组培苗生产概述,热带农业科学,28(6):47-52)
Chen M.H.,Wang P.J.,and Maeda E.,1987,Somatic embryogenesis and plant regeneration in Carica papaya L.tissue culture derived form root explants,Plant Cell Reports,6(5)348-351
Chen Z.H.,2001,studies on the biochemical and molecular marker for papaya(Carica papya L.)sexuality,Thesis for MS.,Fujian Agricultural and forestry University,Supervisor Chen X.J.,pp.16-25(陈中海,2001,番木瓜性别的生化标记与分子标记研究,硕士学位论文,福建农业大学,导师陈晓静,pp.16-25)
DeOliveira J.G.,and Vitória A.P.,2011,Papaya:Nutritional and pharmacological characterization and quality loss duetophysiological disorders an overview,Food Research International,44(5):1306-1313
Fitch M.M.,Manshardt R.M.,Gonsalves D.,Slightom J.L.,and Sanford J.C.,1990,Stable transformation of papaya via microprojectile bombardment,Plant Cell Rep.,9(4):189-194
Gao H.,Yi J.Y.,Bi J.F.,Liu X.,Deng F.M.,and Wu X.Y.,2015Drying characteristics and quality of Carica papaya Linn under Med-and Short-wave infrared radiation and hot air drying,Xiandai Shipin Keji(Modern Food Science and Technology),31(2):190-195,172(高鹤,易建勇,毕金峰,刘璇邓放明,吴昕烨,2015,中短波红外和热风干燥对番木瓜干燥特性及品质的比较,现代食品科技,31(2):190-195172)
Huang J.C.,and Xiao Y.,2003,Biological effects of irradiation on Carica papaya seeds combined with giberrellin,Xinan Nongye Daxue Xuebao(Journal of Southwest Agricultural University),25(3):203-206(黄建昌,肖艳,2003,番木瓜种子的辐射生物学效应研究,西南农业大学学报,25(3)203-206)
Huang J.C.,Xiao Y.,Liang G.S.,Zhao C.X.,and Li H.B.,2004A preliminary study of the breeding of a prsv-resistan strain of papaya(Carica papaya L.),Xinan Nongye Daxue Xuebao(Journal of Southwest University),26(6):25-28(黄建昌,肖艳,梁关生,赵春香,李宏彬,2004,抗环斑型花叶病毒病番木瓜新品种选育研究初报,西南农业大学,26(6):25-28)
Huang J.C.,Ye K.N.,and Li B.J.,1995,Field experiment of hybrid papaya artificial seeds,Zhongshan Daxue Xuebao(Acta Scientiarum Naturalium Universitatis Sunyatseni),34(3):120-121(黄俊潮,叶克难,李宝健,1995,杂种番木瓜人工种子田间试验,中山大学学报(自然科学版),34(3):120-121)
Jia R.,Zhao H.,Huang J.,Kong H.,Zhang Y.,Guo J.,Huang Q.,Guo Y.,Wei Q.,Zuo J.,Zhu Y.J.,Peng M.,and Guo A.,2017,Publisher Correction:use of RNAi technology to develop a PRSV-resistant transgenic papaya,Sci.Rep.,7(1):16390
Kim M.S.,Moore P.H.,Zee F.,Fitch M.M.,Steiger D.L.,Manshardt R.M.,Paull R.E.,Drew R.A.,Sekioka T.,and Ming R.,2002,Genetic diversity of Carica papaya as revealed by AFLP markers,Genome,45(3):503-512
Kumar A.,Basavaraj Y.B.,Kumar P.,Mishra P.,and Singh S.K.,2018,Development of transgene construct using coat protein gene of papaya ringspot virus and its validation,Progressive Agriculture,18(1):92-97
Lin G.X.,Zhou C.Q.,You K.Z.,and Li W.H.,2005,Current research progress and prospect of papaya in China,Guongdong Nongye Kexue(Guangdong Agricultural Sciences),(4):22-24(林冠雄,周常清,游恺哲,李卫红,2005,我国番木瓜育种研究进展与展望,广东农业科学,(4):22-24)
Ming R.,Hou S.,Feng Y.,Yu Q.,Dionne-Laporte A.,Saw J.H.,Senin P.,Wang W.,Ly B.V.,Lewis K.L.,Salzberg S.L.,Feng L.,Jones M.R.,Skelton R.L.,Murray J.E.,Chen C.,Qian W.,Shen J.,Du P.,Eustice M.,Tong E.,Tang H.,Lyons E.,Paull R.E.,Michael T.P.,Wall K.,Rice D.W.,Albert H.,Wang M.L.,Zhu Y.J.,Schatz M.,Nagarajan N.,A-cob R.A.,Guan P.,Blas A.,Wai C.M.,Ackerman C.M.,Ren Y.,Liu C.,Wang J.,Wang J.,Na J.K.,Shakirov E.V.,Haas B.,Thimmapuram J.,Nelson D.,Wang X.,Bowers J.E.,Gschwend A.R.,Delcher A.L.,Singh R.,Suzuki J.Y.,Tripathi S.,Neupane K.,Wei H.,Irikura B.,Paidi M.,Jiang N.,Zhang W.,Presting G.,Windsor A.,Navajas-Pérez R.,Torres M.J.,Feltus F.A.,Porter B.,Li Y.,Burroughs A.M.,Luo M.C.,Liu L.,Christopher D.A.,Mount S.M.,Moore P.H.,Sugimura T.,Jiang J.,Schuler M.A.,Friedman V.,Mitchell-Olds T.,Shippen D.E.,dePamphilis C.W.,Palmer J.D.,Freeling M.,Paterson A.H.,Gonsalves D.,Wang L.,and Alam M.,2008,The draft genome of the transgenic tropical fruit tree papaya(Carica papaya Linnaeus),Nature,452(7190):991-996
Qu Y.,Li W.J.,Zhou L.B.,Wang Z.Z.,Dong X.C.,Yu L.X.,Liu Q.F.,and He J.Y.,2007,Research and application of mutagenic effects in plants irradiated by heavy ion beams,Yuanzihe Wuli Pinlun(Nuclear Physics Review),24(4):294-298(曲颖,李文建,周利斌,王转子,董喜存,余丽霞,刘青芳,何金玉,2007,重离子辐射植物的诱变效应研究及应用,原子核物理评论,24(4):294-298)
Rivera-Pastrana D.M.,Yahia E.M.,and González-Aguilar G.A.,2010,Phenolicand carotenoid profiles of papaya fruit(Carica papaya L.)and their contents under low temperature storage,J.Sci.Food Agric.,90(14):2358-2365
Schweiggert R.M.,Mezger D.,Schimpf F.,Steingass C.B.,and Carle R.,2012,Influence of chromoplast morphology on carotenoid bioaccessibility of carrot,mango,papaya,and tomato,Food Chemistry,135(4):2736-2742
Sheen T.F.,Wang H.L.,and Wang D.N.,1998,Control of papaya ringspot virus by cross protection and cultivation techniques Engei Gakkai Zasshi,67(6):1232-1235
Tripathi S.,Suzuki J.Y.,CarrJ B.J.,McQuate G.T.,Ferreira S.A.Manshardt R.M.,Pitz K.Y.,Wall M.M.,and Gonsalves D.2011,Nutritional composition of rainbow papaya,the firs commercialized transgenic fruit crop,Journal of Food Composition and Analysis,24(2):140-147
Urasaki N.,Tokumoto M.,Tarora K.,Ban Y.,Kayano T.,Tanaka H.,Oku H.,Chinen I.,and Terauchi R.,2002,A male and hermaphrodite specific RAPA marker for papaya(Carica papaya L.),Theor.Appl.Genet.,104(2-3):281-285
Wang F.,Cai S.K.,and Tang Y.F.,2008,Advances in studies on variety breeding and rapid propagation technique of tissue cultured seedings in Carica papaya L.,Guangdong Nongye Kexue(Guangdong Agricultural Sciences),(S):117-121(王芳,蔡时可,汤亚飞,2008,番木瓜品种选育及组培快繁技术研究进展,广东农业科学,(S):117-121)
Wang W.X.,Li C.Y.,Xiang S.Q.,Guo Q.G.,Lin X.L.,and Liang G.L.,2008,Polyploid induced from Carica papaya and morphological characteristics compared between tetraploids and diploids,Guoshu Xuebao(Journal of Fruit Science),25(1):115-118(汪卫星,李春艳,向素琼,郭启高,李晓林,梁国鲁,2008,番木瓜四倍体的诱导及形态学分析,果树学报25(1):115-118)
Xue Y.B.,Zhong K.,Han B.,Gui J.F.,Wang T.,Fu X.D.,He ZH.,Chu C.C.,Tian Z.X.,Chen Z.K.,and Lin S.Y.,2015New chapter of designer breeding in china:update on strategic program of molecular module-based designer breeding systems,Zhongguo Kexueyuan Yuankan(Proceedings of the Chinese Academy of Sciences),30(3):393-402,282(薛勇彪,种康,韩斌,桂建芳,王台,傅向东,何祖华,储成才,田志喜,程祝宽,林少扬,2015,开启中国设计育种新篇章-“分子模块设计育种创新体系”战略性先导科技专项进展,中国科学院院刊,30(3):393-402,282)
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