结合组织芯片技术的人体胃管状腺癌组织太赫兹光谱检测
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摘要
太赫兹波技术在生物医学领域具有广泛应用前景,结合人体胃管状腺癌组织检测,针对太赫兹时域光谱(THz-TDS)组织检测点位与实际病理难以配准的问题,在处理石蜡包埋的胃组织的过程中引入组织芯片技术,为实验样本的精准定位检测提供了可行方法。开展了人体胃正常组织和癌变组织太赫兹检测实验,结合主成分分析方法,对比分析了组织芯片技术配准的实验组与未配准的对照组的太赫兹吸收系数和折射率谱。此外对比研究了采用支持向量机和逻辑回归的太赫兹胃管状腺癌判别方法。研究结果表明,使用组织芯片方法有助于提升组织取材的精准性从而提高癌变样本和正常样本光谱数据的区分效果。
Terahertz wave technique is very prospective in the biomedical application field,where there exists a practical problem that it is hard to register a terahertz time-domain spectroscopy(THz-TDS)detection point and its accurate corresponding position in pathology image.In this paper,complex human gastric tissue is taken as the experimental subject to search for accurate detection approach.This paper uses tissue microarray technology to process paraffin-embedded gastric tissue,and then contrasts the Terahertz absorption coefficient and refractive index spectrum of detection subjects which are divided into registered experimental group and unregistered control group using principal component analysis method.Meanwhile,this paper contrasts the gastric tubular adenocarcinoma THz-TDS discrimination methods using support vector machine and logistic regression respectively.The research result reveals that the detection accuracy brought by tissue microarray technology helps better discriminate tumor samples and normal samples.
Terahertz wave technique is very prospective in the biomedical application field,where there exists a practical problem that it is hard to register a terahertz time-domain spectroscopy(THz-TDS)detection point and its accurate corresponding position in pathology image.In this paper,complex human gastric tissue is taken as the experimental subject to search for accurate detection approach.This paper uses tissue microarray technology to process paraffin-embedded gastric tissue,and then contrasts the Terahertz absorption coefficient and refractive index spectrum of detection subjects which are divided into registered experimental group and unregistered control group using principal component analysis method.Meanwhile,this paper contrasts the gastric tubular adenocarcinoma THz-TDS discrimination methods using support vector machine and logistic regression respectively.The research result reveals that the detection accuracy brought by tissue microarray technology helps better discriminate tumor samples and normal samples.
引文
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[15] Duvillaret L,Garet F,Coutaz J L.IEEE Journal of Selected Topics in Quantum Electronics,1996,2(3):739.
[16] Dorney T D,Baraniuk R G,Mittleman D M.Journal of the Optical Society of America A-Optics Image Science and Vision,2001,18(7):1562.
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[18] Bowman T,Wu Y,Gauch J,et al.Journal of Infrared Millimeter&Terahertz Waves,2017,38(6):766.
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[22] Platt J C.Advances in Large Margin Classifiers,2000,10(4):61.