亲和分离技术中亲和配基的研究进展
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摘要
亲和分离技术在蛋白质的分离纯化过程中发挥着越来越重要的作用,开发合适的配基是亲和分离的关键。按生物大分子亲和配基、小分子亲和配基、金属亲和配基三大类对亲和配基的研究情况进行综述,重点介绍其发展现状、作用机理以及目前存在的主要问题,并对亲和配基未来发展进行展望,旨为将其更好地应用于实际生产。
Affinity separation technology plays an increasingly important role in the proteins separation and purification field.Development of an appropriate ligand is the critical factor for affinity separation technology. In this work,the current research progress of theaffinity ligand is reviewed according to three categories i.e. biological macromolecules affinity ligands,small molecule affinity ligands and metaliron affinity ligand. The current progress of each kind of ligand,its action mechanism and its deficiency are highlighted. Also,the prospect fordevelopment of affinity ligands is also given.
Affinity separation technology plays an increasingly important role in the proteins separation and purification field.Development of an appropriate ligand is the critical factor for affinity separation technology. In this work,the current research progress of theaffinity ligand is reviewed according to three categories i.e. biological macromolecules affinity ligands,small molecule affinity ligands and metaliron affinity ligand. The current progress of each kind of ligand,its action mechanism and its deficiency are highlighted. Also,the prospect fordevelopment of affinity ligands is also given.
引文
[1]胡永红,李凤珠,韩曜平.生物分离工程[M].武汉:华中科技大学出版社,2015.
[2]李杨,韩梅.亲和层析技术在生物科学中的应用及发展[J].生命科学研究,2006,10(1):13-17.
[3]吴璞强.金黄色葡萄球菌蛋白A亲和配基的分子改造与应用[D].无锡:江南大学,2013.
[4]唐思远.人免疫球蛋白G的Fab和Fc片段制备及层析分离研究[D].杭州:浙江大学,2013.
[5]黄波.抗体亲和肽配基的理性设计和色谱表征[D].天津:天津大学,2011.
[6]EI Khoury G,Lowe CR.A biomimetic Protein G affinity adsorbent:an Ugi ligand for immunoglobulins and Fab fragments based on thethird Ig G-binding domain of Protein G[J].Journal of MolecularRecognition,2013,26(4):190-200.
[7]Mei L,Cao B,Yang H,et al.Development of an immunoaffinitychromatography column for selective extraction of a new agonistphenylethylamine A from feed,meat and liver samples[J].JChromatogr B Analyt Technol Biomed Life Sci,2014,945-946:178-84.
[8]王明永,张丽芸,张雅妮,等.联合应用配体和单克隆抗体亲和层析纯化人血浆天然MBL蛋白[J].免疫学杂志,2008(2):119-122.
[9]Hu SD,Liu H,Qiao SY,et al.Development of immunoaffinitychromatographic method for isolating glycinin(11S)from soybeanproteins[J].J Agric Food Chem,2013,61(18):4406-4410.
[10]Ruiz-May E,CataláC,Rose JK,Rose K.N-glycoprotein enrichmentby lectin affinity chromatography[J].Methods Mol Biol,2014,1072:633-643.
[11]杨彦杰,杨军英,张亚磊,等.Con A型差异糖蛋白在大鼠肝再生中的作用研究[J].西北师范大学学报,2013(4):76-82.
[12]王志凤.小黑杨花芽和叶芽糖基化蛋白质组学研究[D].哈尔滨:东北林业大学,2014.
[13]路垚,林华娟,杨基住.凝集素亲和层析技术分离金花茶多糖的研究[J].农业研究与应用,2014(4):1-8.
[14]时照梅,范超,黄俊杰.氧化石墨烯固定化凝集素的制备及在糖蛋白/糖肽富集中的应用[J].色谱,2015(33):116-122.
[15]童贤涛,钟璇,何小云,等.纤维素膜的制备及性能研究[J].高分子通报,2013(10):36-49.
[16]王蕊.疏水色谱膜对混合蛋白的分离性能研究[D].北京:北京化工大学,2015.
[17]符瑞.超支化固定金属离子亲和膜的制备及其吸附性能研究[D].西安:西北大学,2015.
[18]Zeng XF,Ruckenstein E.Cross-linked macroporous chitosananion-exchange membranes for protein separations[J].Journalof Membrane Science,1998(148):195-205.
[19]王苍.高密度糖基化聚丙烯微孔亲和膜的点击化学制备及其蛋白质分分离研究[D].杭州:浙江大学,2011.
[20]朱俐燕.多肽配基定向化固定抗体的研究[D].天津:天津大学,2013.
[21]王荣柱.以四肽为配基的仿生层析和抗体的分离纯化[D].杭州:浙江大学,2015.
[22]赵勇山,郑清川,张红星,等.人类丝氨酸消旋酶的同源模建及其与多肽抑制剂的分子对接[J].物理化学学报,2009,25(3):417-422.
[23]卢慧丽,林东强,姚善泾.亲和仿生层析及在抗体纯化中的应用[J].化工学报,2016,67(9):3523-3535.
[24]董晓燕,曹俊,俞海青,等.多肽亲和配基的固相合成及其亲和作用[J].天津大学学报,2006,39(1):29-33.
[25]Tong HF,Zhang QL,Lin DQ,et al.Molecular recognition of Fcspecific ligands binding onto the consensus binding site of Ig G:insights from molecular simulation[J].Journal of MolecularRecognition,2014(27):501-509.
[26]Chen TX,Nie HL,Shu B.Comparison:Adsorption of papain usingimmobilized dye ligands on affinity membranes[J].Colloids andSurfaces B:Biointerfaces,2009(72):25-31.
[27]Nie HL,Zhu LM.Adsorption of papain with Cibcron BlueF3GA carrying chitosan-coated nylon affinity membranes[J].International Journal of Biological Macromolecule,2007(40):261-267.
[28]Nie HL,Chen TX,Zhu LM.Adsorption of papain on dye affinitymembranes:Isotherm,kinetic,and thermodynamic analysis[J].Separation and Purification Technology,2007(57):121-125.
[29]Li ZH.Affinity adsorption of lysozyme with reactive red 120modified magnetic chitosan microspheres[J].Food Chemistry,2014(145):749-755.
[30]白金山.精氨酸亲和色谱分离纯化超螺旋质粒DNA[D].天津:天津大学,2013.
[31]鞠佳.亲和膜配基的结构和密度对胆红素吸附的影响[J].2013(64):303-310.
[32]徐堃.聚偏氟乙烯接枝氨基酸亲和膜机器内毒素脱出研究[D].杭州:浙江大学,2010.
[33]李琼.固定化金属离子螯合琼脂糖凝胶的制备及其纯化组氨酸标签蛋白的研究[D].南京:南京理工大学,2012.
[34]Ke YM,Chen CI,Chen H,et al.Exploring the complex effects ofmetal ions on D-hydantoinase purification with an immobilizedmetal affinity membrane[J].Journal of the Taiwan Institute ofChemical Engineers,2011(42):735-740.
[35]Eldin MSM.Novel immobilized Cu2+ion grafted cellophanemembranes for affinity separation of His-Tag Chitinase[J].Arabian Journal of Chemistry,2014(45):500-506.
[36]Wu CY,Suen SY,Chen SC,et al.Analysis of protein adsorptionon regenerated cellulose-based immobilized copper ion affinitymembranes[J].Journal of Chromatography A,2003(996):53-70.
[37]Pina AS.Challenges and opportunities in the purification ofrecombinant tagged proteins[J].Biotechnology Advances,2014(32):366-381.
[2]李杨,韩梅.亲和层析技术在生物科学中的应用及发展[J].生命科学研究,2006,10(1):13-17.
[3]吴璞强.金黄色葡萄球菌蛋白A亲和配基的分子改造与应用[D].无锡:江南大学,2013.
[4]唐思远.人免疫球蛋白G的Fab和Fc片段制备及层析分离研究[D].杭州:浙江大学,2013.
[5]黄波.抗体亲和肽配基的理性设计和色谱表征[D].天津:天津大学,2011.
[6]EI Khoury G,Lowe CR.A biomimetic Protein G affinity adsorbent:an Ugi ligand for immunoglobulins and Fab fragments based on thethird Ig G-binding domain of Protein G[J].Journal of MolecularRecognition,2013,26(4):190-200.
[7]Mei L,Cao B,Yang H,et al.Development of an immunoaffinitychromatography column for selective extraction of a new agonistphenylethylamine A from feed,meat and liver samples[J].JChromatogr B Analyt Technol Biomed Life Sci,2014,945-946:178-84.
[8]王明永,张丽芸,张雅妮,等.联合应用配体和单克隆抗体亲和层析纯化人血浆天然MBL蛋白[J].免疫学杂志,2008(2):119-122.
[9]Hu SD,Liu H,Qiao SY,et al.Development of immunoaffinitychromatographic method for isolating glycinin(11S)from soybeanproteins[J].J Agric Food Chem,2013,61(18):4406-4410.
[10]Ruiz-May E,CataláC,Rose JK,Rose K.N-glycoprotein enrichmentby lectin affinity chromatography[J].Methods Mol Biol,2014,1072:633-643.
[11]杨彦杰,杨军英,张亚磊,等.Con A型差异糖蛋白在大鼠肝再生中的作用研究[J].西北师范大学学报,2013(4):76-82.
[12]王志凤.小黑杨花芽和叶芽糖基化蛋白质组学研究[D].哈尔滨:东北林业大学,2014.
[13]路垚,林华娟,杨基住.凝集素亲和层析技术分离金花茶多糖的研究[J].农业研究与应用,2014(4):1-8.
[14]时照梅,范超,黄俊杰.氧化石墨烯固定化凝集素的制备及在糖蛋白/糖肽富集中的应用[J].色谱,2015(33):116-122.
[15]童贤涛,钟璇,何小云,等.纤维素膜的制备及性能研究[J].高分子通报,2013(10):36-49.
[16]王蕊.疏水色谱膜对混合蛋白的分离性能研究[D].北京:北京化工大学,2015.
[17]符瑞.超支化固定金属离子亲和膜的制备及其吸附性能研究[D].西安:西北大学,2015.
[18]Zeng XF,Ruckenstein E.Cross-linked macroporous chitosananion-exchange membranes for protein separations[J].Journalof Membrane Science,1998(148):195-205.
[19]王苍.高密度糖基化聚丙烯微孔亲和膜的点击化学制备及其蛋白质分分离研究[D].杭州:浙江大学,2011.
[20]朱俐燕.多肽配基定向化固定抗体的研究[D].天津:天津大学,2013.
[21]王荣柱.以四肽为配基的仿生层析和抗体的分离纯化[D].杭州:浙江大学,2015.
[22]赵勇山,郑清川,张红星,等.人类丝氨酸消旋酶的同源模建及其与多肽抑制剂的分子对接[J].物理化学学报,2009,25(3):417-422.
[23]卢慧丽,林东强,姚善泾.亲和仿生层析及在抗体纯化中的应用[J].化工学报,2016,67(9):3523-3535.
[24]董晓燕,曹俊,俞海青,等.多肽亲和配基的固相合成及其亲和作用[J].天津大学学报,2006,39(1):29-33.
[25]Tong HF,Zhang QL,Lin DQ,et al.Molecular recognition of Fcspecific ligands binding onto the consensus binding site of Ig G:insights from molecular simulation[J].Journal of MolecularRecognition,2014(27):501-509.
[26]Chen TX,Nie HL,Shu B.Comparison:Adsorption of papain usingimmobilized dye ligands on affinity membranes[J].Colloids andSurfaces B:Biointerfaces,2009(72):25-31.
[27]Nie HL,Zhu LM.Adsorption of papain with Cibcron BlueF3GA carrying chitosan-coated nylon affinity membranes[J].International Journal of Biological Macromolecule,2007(40):261-267.
[28]Nie HL,Chen TX,Zhu LM.Adsorption of papain on dye affinitymembranes:Isotherm,kinetic,and thermodynamic analysis[J].Separation and Purification Technology,2007(57):121-125.
[29]Li ZH.Affinity adsorption of lysozyme with reactive red 120modified magnetic chitosan microspheres[J].Food Chemistry,2014(145):749-755.
[30]白金山.精氨酸亲和色谱分离纯化超螺旋质粒DNA[D].天津:天津大学,2013.
[31]鞠佳.亲和膜配基的结构和密度对胆红素吸附的影响[J].2013(64):303-310.
[32]徐堃.聚偏氟乙烯接枝氨基酸亲和膜机器内毒素脱出研究[D].杭州:浙江大学,2010.
[33]李琼.固定化金属离子螯合琼脂糖凝胶的制备及其纯化组氨酸标签蛋白的研究[D].南京:南京理工大学,2012.
[34]Ke YM,Chen CI,Chen H,et al.Exploring the complex effects ofmetal ions on D-hydantoinase purification with an immobilizedmetal affinity membrane[J].Journal of the Taiwan Institute ofChemical Engineers,2011(42):735-740.
[35]Eldin MSM.Novel immobilized Cu2+ion grafted cellophanemembranes for affinity separation of His-Tag Chitinase[J].Arabian Journal of Chemistry,2014(45):500-506.
[36]Wu CY,Suen SY,Chen SC,et al.Analysis of protein adsorptionon regenerated cellulose-based immobilized copper ion affinitymembranes[J].Journal of Chromatography A,2003(996):53-70.
[37]Pina AS.Challenges and opportunities in the purification ofrecombinant tagged proteins[J].Biotechnology Advances,2014(32):366-381.
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