库拉索芦荟凝胶的晒后修复功效研究
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摘要
通过与甘草酸二钾作对比,研究库拉索芦荟凝胶抑制透明质酸酶的效果,以及通过体外培养角质形成细胞,MTT法测定其经过UVB照射后IL-1α、IL-8分泌变化,研究了库拉索芦荟凝胶的晒后修复功效。结果发现,库拉索芦荟凝胶能够有效抑制透明质酸酶作用,也能使角质形成细胞在经过UVB照射后有效减少炎性细胞因子IL-1α、IL-8的分泌。因此,库拉索芦荟凝胶能够有效修复角质形成细胞由于UVB照射造成的损伤,具备晒后修复功效。
To investigate the after sun repair effects of Aloe Vera gel, by comparing with dipotassium glycyrrhizate, the hyaluronidase inhibition effect of Aloe Vera gel was investigated. The change of the secretion of IL-1α and IL-8 after UVB irradiation was measured by MTT assay through culturing the keratinocyte in vitro. It was found that Aloe Vera gel could effectively inhibit the action of hyaluronidase, and it can also reduce the secretion of in?ammatory cytokine IL-1α and IL-8 from keratinocytes after irradiated by UVB. Therefore, the Aloe Vera gel can effectively repair the damaged keratinocytes caused by UVB irradiation, and it has the after sun repair effects.
To investigate the after sun repair effects of Aloe Vera gel, by comparing with dipotassium glycyrrhizate, the hyaluronidase inhibition effect of Aloe Vera gel was investigated. The change of the secretion of IL-1α and IL-8 after UVB irradiation was measured by MTT assay through culturing the keratinocyte in vitro. It was found that Aloe Vera gel could effectively inhibit the action of hyaluronidase, and it can also reduce the secretion of in?ammatory cytokine IL-1α and IL-8 from keratinocytes after irradiated by UVB. Therefore, the Aloe Vera gel can effectively repair the damaged keratinocytes caused by UVB irradiation, and it has the after sun repair effects.
引文
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[18]王中成.库拉索芦荟多糖促创面愈合作用的研究[D].福州:福建医科大学,2005:38-41.
[19]李晶哲,夏芸,刘柏东,等.芦荟大黄素促进皮肤创伤修复作用机理研究[J].中国中医基础医学杂志,2011,17(11):1260-1263.
[20]王晓玉,刘飞,张莉,等.重组透明质酸酶3种活性测定方法的比较[J].食品与药品,2011,13(1):5-8.
[21]尤建军,王宝玺,曾建英,等.中波紫外线对人角质形成细胞产生白介素-1α和白介素-6的影响[J].临床皮肤科杂志,2003,32(6):332-333.
[22]孙素娇,姚露,罗雯,等.U VA照射度对人工皮肤分泌MMP-1影响的初步研究[J].中国美容医学,2010,19(3):348-350.
[23]CHUNG JH,YOUN SH,KOH WS,et al.Ultraviolet Birradiation-enhanced interleukin(IL)-6 production and mRNAexpression are mediated by IL-1 alpha in cultured human keratinocytes[J].J Invest Dermatol,1996,106(4):715-720.
[24]GRISWOLD DE,CONNOR JR,DALTON BJ,et al.Activation of the IL-1 gene in UV-irradiated mouse skin:association with inflammatory sequelae and pharmacologic intervention[J].JInvest Dermatol,1991,97(6):1019-1023.
[2]黄昭奋.芦荟的起源、分布、生物学特性及其它[J].热带作物科技,1987(6):20.
[3]李云政,秦海元,王青华.国内外芦荟应用研究进展[J].化工进展,2000,19(2):19.
[4]齐永家,李俊强.芦荟栽培与加工利用[M].长春:吉林科学技术出版社,2000:12.
[5]ESHUN K,HE Q.Aloe vera:a valuable ingredient for the food,pharmaceutical and cosmetic industries-a review[J].Crit Rev Food Sci Nutr,2004,44(2):91-96.
[6]JIA Y,ZHAO G,JIA J.Preliminary evaluation:the effects of Aloe ferox Miller and Aloe arborescens Miller on wound healing[J].JEthnopharmacol,2008,120(2):181-189.
[7]冯力,胡正海.芦荟的化学成分及药用价值研究进展[J].西北药学杂志,2008,23(5):332-333.
[8]HASHEMI S A,MADANI S A,ABEDIANKENARI S.The review on properties of Aloe vera in healing of cutaneous wounds[J].Biomed Res Int,2015:714216.
[9]RADHA M H,LAXMIPRIYA N P.Evaluation of biological properties and clinical effectiveness of Aloe vera:a systematic review[J].J Tradit Complement Med,2014,5(1):21-26.
[10]PARK M Y,KWON H J,SUNG M K.Dietary aloin,aloesin,or aloe-gel exerts anti-inflammatory activity in a rat colitis model[J].Life Sci,2011,88(11/12):486-492.
[11]SHIN E,SHIM K S,KONG H,et al.Dietary aloe improves insulin sensitivity via the suppression of obesityinduced inflammation in obese mice[J].Immune Netw,2011,11(1):59-67.
[12]YAGI A,MAKINO I,NISHIOKA,et al.Aloe mannan polysaccharides,from Aloe arborescence var nataensis[J].Planta Medica,1977,31:18.
[13]FARHAD R,CLAUDINE A,ANJANEYALU Y V.Structural studies of the D-glucomanan isolated from Aloe vahombe[J].Carbohyd Res,1983,116:166.
[14]REYNOLDS T,DWECK A C.Aloe Vera leaf gel:a review update[J].Journal of Ethnopharmacology,1999,68:3.
[15]比尔.H.麦卡纳利.制备芦荟产品的方法:美国,89100264[P],1989.
[16]王蜀秀,温远影,王雷,等.芦荟多糖的研究[J],植物学报,1989,31(5):389.
[17]郑荣珍,方仲根,叶海辉.番拉芦荟水溶性多糖的分离与鉴定[J],热带作物学报,2000,21(3):49.
[18]王中成.库拉索芦荟多糖促创面愈合作用的研究[D].福州:福建医科大学,2005:38-41.
[19]李晶哲,夏芸,刘柏东,等.芦荟大黄素促进皮肤创伤修复作用机理研究[J].中国中医基础医学杂志,2011,17(11):1260-1263.
[20]王晓玉,刘飞,张莉,等.重组透明质酸酶3种活性测定方法的比较[J].食品与药品,2011,13(1):5-8.
[21]尤建军,王宝玺,曾建英,等.中波紫外线对人角质形成细胞产生白介素-1α和白介素-6的影响[J].临床皮肤科杂志,2003,32(6):332-333.
[22]孙素娇,姚露,罗雯,等.U VA照射度对人工皮肤分泌MMP-1影响的初步研究[J].中国美容医学,2010,19(3):348-350.
[23]CHUNG JH,YOUN SH,KOH WS,et al.Ultraviolet Birradiation-enhanced interleukin(IL)-6 production and mRNAexpression are mediated by IL-1 alpha in cultured human keratinocytes[J].J Invest Dermatol,1996,106(4):715-720.
[24]GRISWOLD DE,CONNOR JR,DALTON BJ,et al.Activation of the IL-1 gene in UV-irradiated mouse skin:association with inflammatory sequelae and pharmacologic intervention[J].JInvest Dermatol,1991,97(6):1019-1023.