红树蚬与歪红树蚬对短链氯化石蜡胁迫应答的差别
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摘要
【目的】研究红树蚬Polymesoda erosa与歪红树蚬Polymesoda expansa对短链氯化石蜡(Short-chain Chlorinated Paraffins,SCCPs)胁迫应答的差别。【方法】观察两种蚬血细胞的溶酶体中性红保持时间(Neutral Red Retention Time,NRRT)、总抗氧化活力(Total Antioxidant Capacity,T-AOC)、丙二醛(MDA)含量对不同剂量-时间SCCPs的响应。【结果】在低浓度SCCPs胁迫早期,两种蚬NRRT均表现出"毒物兴奋效应",其中红树蚬表现更为明显;T-AOC值、MDA含量表明歪红树蚬对于逆境的抵抗力更强。中、高浓度SCCPs胁迫下两种蚬对SCCPs响应结果相似。【结论】红树蚬与歪红树蚬对SCCPs胁迫应答随剂量-时间变化总体趋势相似。歪红树蚬对逆境的抵抗力略强。
【Objective】To study the different responses of SCCPs(short-chain chlorinated paraffins)stress between Polymesoda erosaand Polymesoda expans.【Methods】The lysosome neutral red retention time(NRRT),total antioxidant capacity(T-AOC)and MDA content of blood of two clams were observed along with the change of the different time and dose SCCPs.【Results】In the early stage of low SCCPs concentration stress,the NRRT of two clams showed hormesis,especially for Polymesoda erosa.T-AOC and MDA content showed that Polymesoda expansa was more resistant to adversity.The responses of two clams to SCCPs were similar under middle and high concentrations of SCCPs stress. 【Conclusion 】 The responses of Polymesoda erosa and Polymesoda expansa to SCCPs stress were similar with the change of dose and time,and the resistance of Polymesoda expansato adversity was slightly stronger.
【Objective】To study the different responses of SCCPs(short-chain chlorinated paraffins)stress between Polymesoda erosaand Polymesoda expans.【Methods】The lysosome neutral red retention time(NRRT),total antioxidant capacity(T-AOC)and MDA content of blood of two clams were observed along with the change of the different time and dose SCCPs.【Results】In the early stage of low SCCPs concentration stress,the NRRT of two clams showed hormesis,especially for Polymesoda erosa.T-AOC and MDA content showed that Polymesoda expansa was more resistant to adversity.The responses of two clams to SCCPs were similar under middle and high concentrations of SCCPs stress. 【Conclusion 】 The responses of Polymesoda erosa and Polymesoda expansa to SCCPs stress were similar with the change of dose and time,and the resistance of Polymesoda expansato adversity was slightly stronger.
引文
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[2]European Community.COMMISSION REGULATION(EU)2015/2030of 13November 2015amending Regulation(EC)No 850/2004of the European Parliament and of the Council onpersistent organic pollutants as regards Annex I[R].European Community,2015.
[3]吴苹,赫巍,张海军,等.短链氯化石蜡限用对我国氯化石蜡产业的影响[J].现代化工,2013,33(1):17-21.WU P,HE W,ZHANG H J,et al.Influence of restricted utilization of short-chain chlorinated paraffins on the chlorinated paraffin industry in China[J].Modern Chemical Industry,2013,33(1):17-21.
[4]陈茹,王亚韡,王璞,等.短链氯化石蜡在台州电子拆解地区的分布特征[J].环境化学,2014,33(6):873-879.CHEN R,WANG Y W,WANG P,et al.Spatial distribution of short chain chlorinated paraffins in soils from Taizhou,an e-waste dismantling area[J].Environmental Chemistry,2014,33(6):873-879.
[5]BUCHER J R,ALISON R H,MONTGOMERY C A,et al.Comparative toxicity and carcinogenicity of two chlorinated paraffins in F344/N rats and B6C3F1 mice[J].Fundamental and Applied Toxicology,1987,9(3):454-468.
[6]SERRONE D M,BIRTLEY R D N,WEIGAND W,et al.Toxicologyof chlorinated paraffins[J].Food and Chemical Toxicology,1987,25(7):553-562.
[7]UNEP,POPs Review Committee.Consideration of draft risk profiles:Short-chained chlorinated paraffins(UN-EP/POPS/POPRC.4/10)[R].Geneva:UNEP,2008.
[8]FIRSK A T,TOMY G T,MUIR D C G.Toxicity of,and polychlorinated alkanes to Japanese medaka(Oryzias latipes)embryos[J].Environmental Toxicology and Chemistry,1999,18(12):2894-2902.
[9]刘丽华,马万里,刘丽艳,等.短链氯化石蜡C10(50.2%Cl)对斑马鱼胚胎的发育毒性[J].哈尔滨工业大学学报,2016,48(8):127-130,140.LIU L H,MA W L,LIU L Y,et al.Study on developmental toxicity of short-chain chlorinated paraffins C10(50.2%Cl)in zebrafish embryos[J].Journal of Harbin Institute of Technology,2016,48(8):127-130,140.
[10]李慧娟.短链氯化石蜡在东海近海环境中的分布及迁移转化研究[D].青岛:中国海洋大学,2013.LI H J.The distribution and migration of short chainchlorinated paraffins in the offshore regions of East China Sea[D].Qingdao:Ocean University of China,2013.
[11]THOMPSON R,MADELEY J.The acute and chronic toxicity of a chlorinated paraffin to themysid shrimp(Mysidopsisbahia)[R].Brixham Report BL/B/2373.Devon,UK:Imperial Chemical Industries PLC,1983.
[12]BROWN D,THOMPSON R S.Phthalates and the aquatic environment:PartⅡThe bioconcentration and depuration of di-2-ethyl-hexyl phthalate(DEHP)and diisodecyl phthalate(DIDP)in mussels(mytilus-edulis)[J].Chemosphere,1982,11(4):427-435.
[13]周浩郎,张俊杰,邢永泽,等.广西红树蚬的分布特征及影响因素分析[J].广西科学,2014,21(2):147-152.ZHOU H L,ZHANG J J,XING Y Z,et al.Characteristics of distribution and the influential factors of mangrove clam,Polymesoda erosa(Solander 1786),in Guangxi[J].Guangxi Sciences,2014,21(2):147-152.
[14]郝再彬,苍晶,徐仲.植物生理实验[M].哈尔滨:哈尔滨工业大学出版社,2004:106-108,113-115.HAO Z B,CANG J,XU Z.Plant physiology experiment[M].Harbin:Harbin Institute of Technology Press,2004:106-108,113-115.
[15]FERNLEY P W,MOORE M N,LOWE D M,et al.Impact of the sea Empress oil spill on lysosomal stability in mussel blood cells[J].Marine Environmental Research,2000,50(1/2/3/4/5):451-455.
[16]BENZIEI F F,STRAIN J J.The ferric reducing ability of plasma as a measure of“antioxidant power”:The FRAP assay[J].Analytical Biochemistry,1996,239(1):70-76.
[17]SMITH P K,KROHN R I,HERMANSON G T,et al.Measurement of protein using bicinchoninicacid[J].Analytical Biochemistry,1985,150(1):76-85.
[18]STEBBING A R D.Hormesis-the stimulation of growth by low levels of inhibitiors[J].Science of Total Environment,1982,22(1):213-234.
[19]HAVELAAR A C,DE GAST I L,SNIJDERS S,et al.Characterization of a heavy metal ion transporter in the lysosomal membrane[J].FEBS Letters,1998,436(2):223-227.
[20]MOORE M N,ALLEN J L,MCVEIGH A,et al.Lysosomal and autophagic reactions as predictive indicators of environmental impact in aquatic animals[J].Autophagy,2006,2(3):217-220.
[21]LOWE D M,MOORE M N,CLARKE K R.Effects of oil on digestive cells in mussels:Quantitative alterations in cellular and lysosomal structure[J].Aquatic Toxicology,1981,1(3/4):213-226.
[22]MOORE M N,CLARKE K R.Use of microstereology and quantitative cytochemistry to determine the effects of crude oil-derived aromatic hydrocarbons on lysosomal structure and function in a marine bivalve mollusk,Mytilus edulis[J].Histochemical Journal,1982,14(5):713-718.
[23]BEBIANNO M J,SERAFIM A,CAMUS L,et al.Antioxidant systems and lipid peroxidation in Bathymodiolu sazoricus from Mid-Atlantic Ridge hydrothermal vent fields[J].Aquatic Toxicology,2005,75(4):354-373.
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