药用植物中UGT家族研究进展
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摘要
尿苷二磷酸葡萄糖醛酸转移酶(UDP-glucuronosyltransferase,UGT)家族是植物体内最大的糖基转移酶家族。编码合成UGT的基因属于UGT基因家族。UGT催化的糖基化反应广泛地存在于药用植物次生代谢物质的合成过程中。作为代谢通路中的下游修饰,供体分子在UGT的催化下,将糖基连接到受体分子上。这一过程往往会改变终产物的理化及生物学性质,最终影响其实际的利用价值及利用方式。本文综述分析了药用植物UGT家族基因挖掘分析、功能验证和生产应用等方面近年来的研究进展。
UDP -glucuronosyltransferase(UGT)family is the largest one of glycosyltransferases family in plants.Genes encoding UGT belong to the UGT gene family. The glycosylation process catalyzed by UGT exists extensively in medicinal plants during the process of the synthesis of secondary metabolites. It is a downstream modification in the meta-bolic pathway catalyzed by UGT,the sugar moiety is transferred from the donor molecules to acceptor. This process of-ten changes the physical,chemical and biological properties of the final product,ultimately affects its medicinal value and the way we use it. In this paper,the UGT gene family in medicinal plants is selected as the research object. From the perspective of its gene mining,functional verification and application,the related research work in recent years is summarized.
UDP -glucuronosyltransferase(UGT)family is the largest one of glycosyltransferases family in plants.Genes encoding UGT belong to the UGT gene family. The glycosylation process catalyzed by UGT exists extensively in medicinal plants during the process of the synthesis of secondary metabolites. It is a downstream modification in the meta-bolic pathway catalyzed by UGT,the sugar moiety is transferred from the donor molecules to acceptor. This process of-ten changes the physical,chemical and biological properties of the final product,ultimately affects its medicinal value and the way we use it. In this paper,the UGT gene family in medicinal plants is selected as the research object. From the perspective of its gene mining,functional verification and application,the related research work in recent years is summarized.
引文
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[14]Qin J J,Sun C Y,Zhang M P,et al.Classification,function and evolution of plant UDP-glycosyltransferase[J].Genomics and Applied Biology,2018,37(1):440-450.秦晶晶,孙春玉,张美萍,等.植物UDP-糖基转移酶分类、功能以及进化[J].基因组学与应用生物学,2018,37(1):440-450.
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[16]Coutinho P M,Deleury E,Davies G J,et al.An evolv-ing hierarchical family classification for glycosyltransfer-ases[J].J Mol Biol,2003,328(2):307-317.
[17]Mackenzie P I,Owens I S,Burchell B,et al.The UDP glycosyltransferase gene superfamily:recommend-ed nomenclature update based on evolutionary diver-gence[J].Pharmacogenetics,1997,7(4):255-269.
[18]Ross J,Li Y,Lim E K,et al.Higher plant glycosyl-transferases[J].Genome Biol,2001,2(2):1-6.
[19]Luo Y,Liu X G,Zhou Z Q.Research progress on methods for isolating the gene of plant glycosyltransfer-ase,and its biological functions[J].Biotechnol Bull,2016,32(12):34-39.罗燕,刘小刚,周志钦.植物糖基转移酶基因的分离方法及其生物学功能研究进展[J].生物技术通报,2016,32(12):34-39.
[20]Xing L P,He L Q,Xiao J,et al.An UDP-glucosyl-transferase gene from barley confers disease resistance to Fusarium head blight[J].Plant Mol Biol Rep,2017,35(2):224-236.
[21]Ji K.ABA-Glucosyltransferase involved in fruit ripening[D].Beijing:China Agricultural University,2014.纪凯.脱落酸葡糖基转移酶参与果实成熟过程[D].北京:中国农业大学,2014.
[22]Xu H S.Preliminary study on screening,cloning and genetic transformation of P450 and UGT gene related to begonia saponins synthesis[D].Beijing:Peking Union Medical College,2013.徐浩森.北柴胡皂苷合成相关P450、UGT基因的筛选、克隆及遗传转化初步研究[D].北京:北京协和医学院,2013.
[23]Chang K.Cloning of UDPGT gene from Rhodiola crenulata and the response of hairy root after different treatments[D].Chongqing:Southwest University,2013.常凯.大花红景天UDPGT基因克隆及发根对不同诱导子的响应[D].重庆:西南大学,2013.
[24]Liang H C,Wang Q H,Gong T,et al.The basic strat-egies and research advances in the studies on glycosyl-transferases involved in ginsenoside biosynthesis[J].Acta Pharm Sin,2015,50(2):148-153.梁会超,王庆华,巩婷,等.人参皂苷生物合成相关糖基转移酶研究基本策略及进展[J].药学学报,2015,50(2):148-153.
[25]Zhang C L,Chen P,Zhong Y M,et al.Full length c DNA cloning and expression analysis of UFGT gene from Ginkgo biloba[J].Acta Horticul Sin,2012,39(10):1 903-1 912.张传丽,陈鹏,仲月明,等.银杏类黄酮糖基转移酶基因全长序列克隆及表达分析[J].园艺学报,2012,39(10):1 903-1 912.
[26]Li Y.UGT metabolism of luteolin,orientin and isoori-entin and BCRP regulation on the UGT metabolism[D].Guangzhou:Southern Medical University,2013.李烨.木犀草素、荭草苷和异荭草苷的UGT代谢机理及BCRP外排转运蛋白调控其代谢的机制研究[D].广州:南方医科大学,2013.
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