新鲜椰衣纤维表面半纤维素含量的测定及去除
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摘要
新鲜椰衣纤维表面粘附有一定量结构疏松的椰糠,椰糠的主要成分为半纤维素和木聚糖类,既不利于椰衣纤维的改性,也不利于用做固定化酶的载体材料,必须将其除去。采用稀硫酸加热水解,使新鲜椰衣纤维表面粘附的半纤维素转化为可溶的木糖和低聚木糖,以木糖为标准,3,5-二硝基水杨酸(DNS)为显色剂,采用分光光度法间接测定新鲜椰衣纤维中半纤维的含量,以考察椰衣纤维表面半纤维去除的效果。结果表明,当硫酸溶液浓度1.3 mol/L、水解温度102℃、水解时间2 h,固液比为1∶50时,半纤维素的去除效果最佳,新鲜椰衣纤维表面粘附的半纤维素量为14.58%,通过红外光谱和扫描电镜等可观察椰衣纤维表面的变化,重现性试验及标准回收试验证明该方法效果良好。
Fresh coconut fiber has a certain amount of loose coconut bran on its surface. The main components of coco coir are hemicellulose and pentosan, not conducive to modifying coir fiber, must be removed. Dilute sulphuric acid was used to remove the hemicellulose bran on the surface of fresh coconut fiber, and the content of hemicellulose was determined by spectrophotometry. The effect of hemicellulose removal on coir fiber was investigated. The results showed the highest removal of hemicellulose could be achieved when hydrolyzed for 2 h in 1.3 mol/L sulfuric acid with a solid-liquid ratio 1 ∶ 5 under 102 ℃. Under the above conditions the hemicellulose adhesion to the surface of semi fresh coconut fiber was 14.58%. Reproducibility test and standard recovery test confirmed the method was effective. The change of the surface of coconut fiber was observed under infrared scanning electron microscope.
Fresh coconut fiber has a certain amount of loose coconut bran on its surface. The main components of coco coir are hemicellulose and pentosan, not conducive to modifying coir fiber, must be removed. Dilute sulphuric acid was used to remove the hemicellulose bran on the surface of fresh coconut fiber, and the content of hemicellulose was determined by spectrophotometry. The effect of hemicellulose removal on coir fiber was investigated. The results showed the highest removal of hemicellulose could be achieved when hydrolyzed for 2 h in 1.3 mol/L sulfuric acid with a solid-liquid ratio 1 ∶ 5 under 102 ℃. Under the above conditions the hemicellulose adhesion to the surface of semi fresh coconut fiber was 14.58%. Reproducibility test and standard recovery test confirmed the method was effective. The change of the surface of coconut fiber was observed under infrared scanning electron microscope.
引文
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[2]廖双泉,马凤国,邵自强,等.椰衣纤维的蒸汽爆破处理技术[J].热带作物学报,2003,3(1):18-20.
[3]肖红,易美华.椰子的开发利用[J].海南大学学报(自然科学版),2003,21(2):183-188.
[4]林尤河,谢国干,张海林,等.椰子的生态特性与生产基地的选择[J].海南大学学报(自然科学版),2000,18(2):155-158.
[5]李志合.生物质组分及温度对闪速热解挥发分的影响[J].燃料化学学报,2005,33(4):502-505.
[6]刘国明.玉米芯半纤维素水解液的制备及利用木糖发酵产乙醇的研究[D].哈尔滨:黑龙江大学,2009.
[7]Azhar S,Henriksson G,Theliander H,et al.Extraction of hemicelluloses from fiberized spruce wood[J].Carbohydr Polym,2015(117):19-24.
[8]Pino G H,Mesquita L M S D,Torem M L,et al.Biosorption of cadmium by green coconut shell powder[J].Minerals Engineering,2006,19(5):380-387.
[9]彭锋.农林生物质半纤维素分离纯化、结构表征及化学改性的研究[D].广州:华南理工大学,2010.
[10]李玲.椰壳半纤维素的提取及表征[D].海口:海南大学,2012.
[11]王政,黄广民,赵斌,等.椰衣半纤维素的提取及其结构表征[J].食品科学,2017,38(14):245-249.
[12]李环,陆佳平,王登进.DNS法测定山楂片中还原糖含量的研究[J].食品工业科技,2013,34(18):75-77.
[13]王俊刚,张树珍,杨本鹏,等.3,5-二硝基水杨酸(DNS)法测定甘蔗茎节总糖和还原糖含量[J].甘蔗糖业,2008(5):45-49.
[14]顾春菊,马敬红,李兰.傅里叶变换红外光谱法定量分析氧化纤维素中的羧基含量[J].分析仪器,2005(3):39-41.
[15]杨瑞林,杨艳萍.扫描电镜粉末样品的制备[J].山西师范大学学报(自然科学版),2002,16(4):44-46.