甘草、茯苓对施氏鲟热应激抗氧化能力的影响
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摘要
为探究中草药对施氏鲟热应激抗氧化能力的影响,在水温22℃条件下,采用口灌给药的方法研究了甘草和茯苓两种中药单方对1+龄施氏鲟(鱼体平均质量:0.08～0.10 kg)抗氧化能力的影响。每组30尾施氏鲟,甘草和茯苓的干质量分别按0.24 g/kg、0.3 g/kg体质量给药剂量进行称取,经熬煮浓缩制成水煎剂后,每日口灌甘草、茯苓水煎剂一次,口灌等量蒸馏水作对照,连续给药21 d。给药结束后,每组取15尾鱼在高温30℃下热刺激2 h后,与22℃给药组同时采样。测定施氏鲟血清、肝脏及红细胞中的丙二醛(MDA),一氧化氮(NO)含量以及超氧化物歧化酶(SOD),一氧化氮合酶(NOS)活性的变化。结果表明:常温条件下,茯苓组肝脏中NOS活性极显著(P<0.01)高于对照组。高温条件下,甘草组红细胞中SOD活性极显著(P<0.01)高于对照组。常温和高温应激后,茯苓组肝脏中SOD活性最高,甘草组血清、肝脏中MDA含量最低。研究表明:甘草组可促进机体对高温热应激做出良好的反应,利于提高鱼体热应激耐受能力。
Juvenile Amur sturgeons Acipenser schrenckii with body weight of(0.08～0.10)kg were adminis?trated orally with Chinese herbal medicine decoction of Glycyrrhiza uralensis or Poria cocos at a dose 0.24 g/kg body weight and 0.3 g/kg body weight per day for 21 days,and the Amur sturgeons in the control group were giv?en oral administration of distilled water. After the drug administration,15 Amur sturgeons in each group were thermally stimulated at 30 ℃ for 2 h and then the blood and liver were sampled together with the experimental groups at 22 ℃.And then the activities of SOD,NOS and the contents of MDA,NO in blood and liver were deter?mined.The activity of NOS in the liver of Poria cocos was obviously higher than that in the control group exposed to normal water temperature(P<0.01).And the activity of SOD in the red blood cells of Glycyrrhiza uralensis was obviously higher than that in the high water temperature control group(P<0.01).After normal temperature and high temperature stress,the maximal SOD activity was found in the liver of the Amur sturgeons with oral ad?ministration of Poria cocos and the minimal MDA content was found in the serum or liver of the Amur sturgeons with oral administration of Glycyrrhiza uralensis.Glycyrrhiza uralensis enables the body to respond better in heat stress,which is beneficial to improve the tolerance to heat stress of Amur sturgeon.
Juvenile Amur sturgeons Acipenser schrenckii with body weight of(0.08～0.10)kg were adminis?trated orally with Chinese herbal medicine decoction of Glycyrrhiza uralensis or Poria cocos at a dose 0.24 g/kg body weight and 0.3 g/kg body weight per day for 21 days,and the Amur sturgeons in the control group were giv?en oral administration of distilled water. After the drug administration,15 Amur sturgeons in each group were thermally stimulated at 30 ℃ for 2 h and then the blood and liver were sampled together with the experimental groups at 22 ℃.And then the activities of SOD,NOS and the contents of MDA,NO in blood and liver were deter?mined.The activity of NOS in the liver of Poria cocos was obviously higher than that in the control group exposed to normal water temperature(P<0.01).And the activity of SOD in the red blood cells of Glycyrrhiza uralensis was obviously higher than that in the high water temperature control group(P<0.01).After normal temperature and high temperature stress,the maximal SOD activity was found in the liver of the Amur sturgeons with oral ad?ministration of Poria cocos and the minimal MDA content was found in the serum or liver of the Amur sturgeons with oral administration of Glycyrrhiza uralensis.Glycyrrhiza uralensis enables the body to respond better in heat stress,which is beneficial to improve the tolerance to heat stress of Amur sturgeon.
引文
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