社区不同年龄段女性端粒长度改变规律及其衰老分值变化趋势
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摘要
【目的】探索社区不同年龄段女性端粒长度改变规律及其衰老分值的变化特点。【方法】从南昌市区多家三甲医院和部分社区卫生服务中心健康体检人群中按年龄分层随机抽取20岁以上女性作为研究对象,进行端粒相对长度检测和分析,同时采用《生理-心理-社会三维人体衰老度量表(PPSHAS)》量表测量其衰老分值。【结果】本次研究共获得有效样本406例,不同年龄组的端粒相对长度的差异有统计学意义(P<0.001),端粒相对长度随年龄增加而缩短,端粒长度(Y)与年龄(X)的相关性分析显示呈中高度负相关(r=-0.623,<0.001),回归方程为:Y=-0.012X+1.984(P<0.001,R2=0.400);衰老分值随年龄的增长而增高,且在40～、60～岁组有明显上升趋势,端粒相对长度则有明显下降趋势,提示两者有一定的契合度,衰老分值有助于对女性衰老的深入研究。【结论】健康女性外周血白细胞端粒长度随年龄增加而缩短,端粒相对长度与年龄呈中高度负相关,各年龄组端粒长度差异不同,端粒相对长度与衰老分值在40～、60～岁组的变化趋势提示了女性在该阶段快速衰老,为女性老年问题的研究提供参考。
【Objective】To explore the changing rules of telomere length and the scores trend in different aging groups of women.【Methods】 The 20-year-old and above healthy women were randomly selected from several hospitals of Nanchang University. Peripheral blood samples were collected as test samples,relative length of telomere was detected and analyzed. Meanwhile,the aging score was measured by PPSHAS scale.【Results】A total of 406 effective samples were obtained in this study,and the difference of telomere relative length in different age groups was statistically significant(P<0.001).The results showed that the relative length of telomere shortened with age,there is a highly negative correlation between telomere length(Y)and age(X;r=-0.623,P<0.001). The regression equation is Y=-0.012 X+1.984(P<0.001,R2=0.400). Scores increased with age,and there was a distinct upward trend in 40～ and 60～ years old groups. The relative length of telomere decreased significantly,suggesting that there was a certain degree of agreement between them.Scores contributed to further research on female senescence.【Conclusion】 The telomere length of peripheral blood leukocytes in healthy women is shortened with age,and the relative length of telomere is negatively correlated with age.The change trend of telomere length and senescence score in 40～ and 60～ age groups suggests that women are rapidly aging at this stage and provide reference for the study of women′s elderly problem.
【Objective】To explore the changing rules of telomere length and the scores trend in different aging groups of women.【Methods】 The 20-year-old and above healthy women were randomly selected from several hospitals of Nanchang University. Peripheral blood samples were collected as test samples,relative length of telomere was detected and analyzed. Meanwhile,the aging score was measured by PPSHAS scale.【Results】A total of 406 effective samples were obtained in this study,and the difference of telomere relative length in different age groups was statistically significant(P<0.001).The results showed that the relative length of telomere shortened with age,there is a highly negative correlation between telomere length(Y)and age(X;r=-0.623,P<0.001). The regression equation is Y=-0.012 X+1.984(P<0.001,R2=0.400). Scores increased with age,and there was a distinct upward trend in 40～ and 60～ years old groups. The relative length of telomere decreased significantly,suggesting that there was a certain degree of agreement between them.Scores contributed to further research on female senescence.【Conclusion】 The telomere length of peripheral blood leukocytes in healthy women is shortened with age,and the relative length of telomere is negatively correlated with age.The change trend of telomere length and senescence score in 40～ and 60～ age groups suggests that women are rapidly aging at this stage and provide reference for the study of women′s elderly problem.
引文
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[8] Seger W. The rediscovery of medicine:philosophyand value of the International Classification ofFunctioning,Disability and Health(ICF)[J]. EPhys,2018,10(3):6426-6429.
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[12]刘冬岩,董联玲.女性衰老过程中不同阶段的衰老机理及其防治思路[J].中医杂志,2002,43(11):862-864.Liu DY,Dong LL. Aging mechanism and preventivemeasures in different stages of female senescence[J]. J Tradit Chin Med,2002,43(11):862-864.
[13]龙耀,周璇,邓鹏飞,等.基于改良德尔菲法的健康人生理性衰老量表构建[J].中南大学学报(医学版),2016,41(4):422-426.Long Y,Zhou X,Deng PF,et al. Construction of aphysiological aging scale for healthy people basedon a modified Delphi method[J]. J Cent South Univ(Med Sci),2016,41(4):422-426.
[14]黄河浪,刘星,李翔,等.人体衰老认识与测试指标(量表)构建的理论思维与方法[J].中华疾病控制杂志,2018,22(1):85-88.Huang HL,Liu X,Li X,et al. Theoretical thinkingand method of cognition of human aging and test(Scale)construction[J]. Chin J Dis Control Prev,2018,22(1):85-88.
[15]Ritz-Timme S,Cattaneo C,Collins MJ,et al. Ageestimation:the state of the art in relation to thespecific demands of forensic practise[J]. Int J LegalMed,2000,113(3):129-136.
[16]Friedrich U,Griese E,Schwab M,et al. Telomerelength in different tissues of elderly patients[J].Mech Ageing Dev,2000,119(3):89-99.
[17]Nielsen B R, Linneberg A, Bendix L, et al.Association between leukocyte telomere length andbone mineral density in women 25-93 years of age[J]. Exp Gerontol,2015,66:25-31.
[18]林菲,周跃平,刘伟新,等.女性衰老特点及主要影响因素的研究概况[J].中国老年学杂志,2017,37(3):757-758.Lin F,Zhou YP,Liu WX,et al. Research overviewof female aging characteristics and main influencingfactors[J]. Chin J Gerontol,2017,37(3):757-758.
[19]陈华燕. 1200例女性更年期心理状况调查及护理对策研究[J].实用临床医药杂志,2016,20(14):108-110;117.Chen HY. Psychological status and nursingcountermeasures of 1200 cases with menopause[J].J Clin Med Pract,2016,20(14):108-110;117.
[20]Kanning M, Schlicht W. A bio-psycho-socialmodel of successful aging as shown through thevariable“physical activity”[J]. Eur Rev AgingPhys A,2008,5(2):79-87.
[21]吴华裕,罗晓秋,韩焕钦,等.长寿家系的白细胞端粒长度[J].中国老年学杂志,2015,35(9):2325-2327.Wu HY,Luo XQ,Han HQ,et al. Family-basedstudy on the leukocyte telomere length of long-livedindividuals[J]. Chin J Gerontol,2015,35(9):2325-2327.
[2] Lapham K,Kvale M N,Lin J,et al. Automatedassay of telomere length measurement andinformatics for 100,000 subjects in the GeneticEpidemiology Research on Adult Health and Aging(GERA)Cohort[J]. Genetics,2015,200(4):1061-72.
[3] Gardner M,Bann D,Wiley L,et al. Gender andtelomere length:systematic review and meta-analysis[J]. Exp Gerontol,2014,51(1):15-27.
[4] Guan JZ,Maeda T,Sugano M,et al. Change in thetelomere length distribution with age in the Japanesepopulation[J]. Mol Cell Biochem,2007,304(12):35360.
[5] Unryn BM,Cook LS,Raibowol KT. Paternal age ispositively linked to telomere length of children[J].Aging Cell,2005,4(1):97101.
[6] Der G,Batty G D,Benzeval M,et al.Is telomerelength a biomarker for aging:cross-sectionalevidence from the west of Scotland?[J]. PLoS One,2012,7(9):e45166.
[7] Flores I,Blasco M A. The role of telomeres andtelomerase in stem cell aging[J]. Febs Letters,2010,584(17):3826-3830.
[8] Seger W. The rediscovery of medicine:philosophyand value of the International Classification ofFunctioning,Disability and Health(ICF)[J]. EPhys,2018,10(3):6426-6429.
[9]中华医学会老年医学分会,中华老年医学杂志编辑部.中国健康老年人标准(2013)[J].中华老年医学杂志,2013,32(8):39.Geriatrics Branch of the Chinese MedicalAssociation,Editorial Department of the ChineseJournal of Geriatrics. Standard of healthy elderly inChina(2013)[J].Chin J Geriatr,2013,32(8):39.
[10]Gorsuch RL. Factor analysis[M]. London:Routledge,1983:395-412.
[11]政晓果,鞠振宇,肖峰,等.端粒与衰老的流行病学研究进展[J].中国老年学杂志,2009,29(21):2831-2834.Zheng XG,Ju ZY,Xiao F,et al. Advances in theepidemiology of telomere and aging[J]. Chin JGerontol,2009,29(21):2831-2834.
[12]刘冬岩,董联玲.女性衰老过程中不同阶段的衰老机理及其防治思路[J].中医杂志,2002,43(11):862-864.Liu DY,Dong LL. Aging mechanism and preventivemeasures in different stages of female senescence[J]. J Tradit Chin Med,2002,43(11):862-864.
[13]龙耀,周璇,邓鹏飞,等.基于改良德尔菲法的健康人生理性衰老量表构建[J].中南大学学报(医学版),2016,41(4):422-426.Long Y,Zhou X,Deng PF,et al. Construction of aphysiological aging scale for healthy people basedon a modified Delphi method[J]. J Cent South Univ(Med Sci),2016,41(4):422-426.
[14]黄河浪,刘星,李翔,等.人体衰老认识与测试指标(量表)构建的理论思维与方法[J].中华疾病控制杂志,2018,22(1):85-88.Huang HL,Liu X,Li X,et al. Theoretical thinkingand method of cognition of human aging and test(Scale)construction[J]. Chin J Dis Control Prev,2018,22(1):85-88.
[15]Ritz-Timme S,Cattaneo C,Collins MJ,et al. Ageestimation:the state of the art in relation to thespecific demands of forensic practise[J]. Int J LegalMed,2000,113(3):129-136.
[16]Friedrich U,Griese E,Schwab M,et al. Telomerelength in different tissues of elderly patients[J].Mech Ageing Dev,2000,119(3):89-99.
[17]Nielsen B R, Linneberg A, Bendix L, et al.Association between leukocyte telomere length andbone mineral density in women 25-93 years of age[J]. Exp Gerontol,2015,66:25-31.
[18]林菲,周跃平,刘伟新,等.女性衰老特点及主要影响因素的研究概况[J].中国老年学杂志,2017,37(3):757-758.Lin F,Zhou YP,Liu WX,et al. Research overviewof female aging characteristics and main influencingfactors[J]. Chin J Gerontol,2017,37(3):757-758.
[19]陈华燕. 1200例女性更年期心理状况调查及护理对策研究[J].实用临床医药杂志,2016,20(14):108-110;117.Chen HY. Psychological status and nursingcountermeasures of 1200 cases with menopause[J].J Clin Med Pract,2016,20(14):108-110;117.
[20]Kanning M, Schlicht W. A bio-psycho-socialmodel of successful aging as shown through thevariable“physical activity”[J]. Eur Rev AgingPhys A,2008,5(2):79-87.
[21]吴华裕,罗晓秋,韩焕钦,等.长寿家系的白细胞端粒长度[J].中国老年学杂志,2015,35(9):2325-2327.Wu HY,Luo XQ,Han HQ,et al. Family-basedstudy on the leukocyte telomere length of long-livedindividuals[J]. Chin J Gerontol,2015,35(9):2325-2327.