出芽短梗霉固态发酵啤酒糟制备阿魏酰低聚糖和膳食纤维工艺研究
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摘要
以啤酒糟为试验材料,研究固态发酵制备阿魏酰低聚糖和膳食纤维的最佳发酵工艺条件。以阿魏酰低聚糖和可溶性膳食纤维含量为评价指标,选择木聚糖、尿素、磷酸二氢钾为影响因素,通过正交试验确定最佳固态培养基配方。在接种量、发酵时间和发酵温度3个单因素试验的基础上,利用响应面法优化出芽短梗霉发酵啤酒糟的发酵工艺条件。结果表明,固态发酵的最佳培养基配方为木聚糖6%、尿素4%和磷酸二氢钾1%;最佳固态发酵条件为接种量12%、发酵时间4 d和发酵温度29℃。在此优化条件下,阿魏酰低聚糖含量和可溶性膳食纤维含量都达到最高,分别为37.67μmol/L和23.76%。
Using brewer's spent grain as raw material, the optimum fermentation process conditions for the preparation of feruloyl oligosaccharides and dietary fiber by solid-state fermentation were studied. Taking the content of feruloyl oligosaccharides and soluble dietary fiber as evaluation indexes, the xylan, urea and KH_2PO_4 as influencing factors, the optimum formula of solid medium was determined by orthogonal tests. Based on the results of three single factors including inoculum, fermentation time, and temperature, the fermentation process conditions of brewer's spent grains by Aureobasidium pullulans were optimized by response surface methodology. The results showed that the optimum formula of medium for solid-state fermentation was as follows: xylan 6%, urea 4%, and KH_2PO_4 1%. The optimum solid-state fermentation conditions were as follows: inoculum 12%,fermentation time 4 d and temperature 29 ℃. Under these optimized conditions, the content of feruloyl oligosaccharides and dietary fiber was 37.67 μmol/L and 23.76%, respectively.
Using brewer's spent grain as raw material, the optimum fermentation process conditions for the preparation of feruloyl oligosaccharides and dietary fiber by solid-state fermentation were studied. Taking the content of feruloyl oligosaccharides and soluble dietary fiber as evaluation indexes, the xylan, urea and KH_2PO_4 as influencing factors, the optimum formula of solid medium was determined by orthogonal tests. Based on the results of three single factors including inoculum, fermentation time, and temperature, the fermentation process conditions of brewer's spent grains by Aureobasidium pullulans were optimized by response surface methodology. The results showed that the optimum formula of medium for solid-state fermentation was as follows: xylan 6%, urea 4%, and KH_2PO_4 1%. The optimum solid-state fermentation conditions were as follows: inoculum 12%,fermentation time 4 d and temperature 29 ℃. Under these optimized conditions, the content of feruloyl oligosaccharides and dietary fiber was 37.67 μmol/L and 23.76%, respectively.
引文
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[19]邬建国,周帅,张晓昱,等.采用药用真菌液态发酵甘薯渣获得膳食纤维的发酵工艺研究[J].食品与发酵工业,2005,31(7):42-44.
[20]李俊杰,管轶男,管斌,等.高生物量富硒产朊假丝酵母培养基优化[J].中国酿造,2018,37(1):74-78.
[2]熊霞,施国中,李淑兰,等.啤酒糟产沼气潜力试验研究[J].中国沼气,2017,35(4):33-35.
[3]KHIDIZR K M,AGAMUTHU P,ABDULLAH N.Brewery spent grain:chemical characteristics and utilization as an enzyme substrate[J].Malays J Sci,2010,29(1):41-51.
[4]焦昆鹏,朱文学,马丽苹,等.玉米麸皮发酵法制备阿魏酰低聚糖及膳食纤维的研究[J].食品科技,2014(8):172-176.
[5]肖连冬,程爽.平菇-650液态发酵啤酒糟获取膳食纤维的培养基优化[J].中国酿造,2015,34(9):67-70.
[6]李雄.利用黑曲霉固态发酵啤酒糟生产饲料复合酶制剂及其应用的研究[D].无锡:江南大学,2009.
[7]崔堂兵,郭勇,郑穗平.出芽短梗霉的研究进展[J].工业微生物,2002,32(2):41-47.
[8]解春艳.茶薪菇发酵制备麦麸膳食纤维与阿魏酰低聚糖及其生物活性研究[D].南京:南京农业大学,2010.
[9]CHRISTOV L P,MYBURGH J,TONDER A V,et al.Hydrolysis of extracted and fibre-bound xylan with Aureobasidium pullulans enzymes[J].J Biotechnol,1997,55(1):21-29.
[10]LI H,CHI Z,WANG X,et al.Purification and characterization of extracellular amylase from the marine yeast Aureobasidium pullulans N13d and its raw potato starch digestion[J].Enzyme Microb Tech,2007,40(5):1006-1012.
[11]KUMAR C G,TAKAGI H.Microbial alkaline proteases:from a bioindustrial viewpoint[J].Biotechnol Adv,1999,17(7):561.
[12]KUDANGA T,MWENJE E.Extracellular cellulase production by tropical isolates of Aureobasidium pullulans[J].Can J Microbiol,2005,51(9):773.
[13]刘畅.出芽短梗霉的发酵条件及其糖酵解研究[D].无锡:江南大学,2012.
[14]余晓红.出芽短梗霉发酵麦麸制备阿魏酰低聚糖及其生物活性研究[D].南京:南京农业大学,2012.
[15]冯志勇,王志强,郭力刚.秀珍菇生物学特性研究[J].食用菌学报,2003,10(3):11-16.
[16]YUAN X,WANG J,YAO H.Antioxidant activity of feruloylated oligosaccharides from wheat bran[J].Food Chem,2005,90(4):759-764.
[17]PROSKY L,ASP N G,FURDA I,et al.Determination of total dietary fiber in foods and food products:collaborative study[J].J Assoc off Anal Chem,1985,68(4):677.
[18]金信江.固态发酵法制取麦麸膳食纤维技术研究[D].南京:南京农业大学,2009.
[19]邬建国,周帅,张晓昱,等.采用药用真菌液态发酵甘薯渣获得膳食纤维的发酵工艺研究[J].食品与发酵工业,2005,31(7):42-44.
[20]李俊杰,管轶男,管斌,等.高生物量富硒产朊假丝酵母培养基优化[J].中国酿造,2018,37(1):74-78.
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