褐环乳牛肝菌对樟子松和油松根际土壤真菌多样性的影响
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摘要
为了解褐环乳牛肝菌Suillusluteus对樟子松Pinussylvestrisvar.mongolica和油松Pinus tabulaeformis根际土壤真菌群落结构的影响,采用Illumina MiSeq高通量测序,对两种松树接种S. luteus与对照组的根际土壤真菌群落结构进行研究。结果显示,4个样品共获得原始序列347681条,归为5个真菌门。综合各土壤样品真菌Alpha多样性指数及OTUs-Venn图,发现接种S.luteus后樟子松和油松根际土壤真菌相对丰度与对照存在一定差异。群落结构分析表明,接种S.luteus提高了樟子松和油松根际土壤担子菌和壶菌的相对丰度,抑制了子囊菌门真菌。优势属由原来的Geopora转变为Suillus。通过PCA分析与NMDS分析发现接种S. luteus后樟子松和油松根际土壤真菌群落差异性增加,且接种S. luteus后樟子松根际土壤真菌群落结构相似性显著高于油松根际土壤真菌群落结构相似性。接种S.luteus的樟子松和油松土壤真菌丰富度降低、多样性增加,接种S.luteus改变了樟子松和油松根际土壤真菌群落结构和优势真菌种群。试验结果为樟子松和油松促生真菌菌种筛选和育苗工作提供了依据。
Illumina MiSeq high throughput sequencing technology was used to analyze the fungal community structure of rhizosphere soil of Pinus sylvestris var. mongolica and P. tabulaeformis inoculated with Suillus luteus. A total of 347 681 raw data were obtained from four test samples, which were classified into five phyla.Based on Alpha diversity index and OTUs-Venn map of the soil samples, it was found that the relative abundance of fungi in the rhizosphere soil of two pine trees inoculated with S. luteus were different from that uninoculated with the fungus. The community structure analysis showed that S. luteus increased the relative abundance of basidiomycetes and chytridiomycetes and inhibited ascomycotes. The dominant genus was changed from Geopora to Suillus. Suillus luteus inoculation increased the difference of fungal community in the rhizosphere soil, and the fungal community similarity in P. sylvestris var. mongolica was significantly higher than that in P. tabulaeformis as a result of PCA and NMDS analysis. S. luteus inoculation decreased the soil fungal richness and increased the soil fungal diversity, and changed the soil fungal community structure and the dominant species composition.
Illumina MiSeq high throughput sequencing technology was used to analyze the fungal community structure of rhizosphere soil of Pinus sylvestris var. mongolica and P. tabulaeformis inoculated with Suillus luteus. A total of 347 681 raw data were obtained from four test samples, which were classified into five phyla.Based on Alpha diversity index and OTUs-Venn map of the soil samples, it was found that the relative abundance of fungi in the rhizosphere soil of two pine trees inoculated with S. luteus were different from that uninoculated with the fungus. The community structure analysis showed that S. luteus increased the relative abundance of basidiomycetes and chytridiomycetes and inhibited ascomycotes. The dominant genus was changed from Geopora to Suillus. Suillus luteus inoculation increased the difference of fungal community in the rhizosphere soil, and the fungal community similarity in P. sylvestris var. mongolica was significantly higher than that in P. tabulaeformis as a result of PCA and NMDS analysis. S. luteus inoculation decreased the soil fungal richness and increased the soil fungal diversity, and changed the soil fungal community structure and the dominant species composition.
引文
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Bueis T,Turrión MB,Bravo F,Pando V,Muscolo A,2018.Factors determining enzyme activities in soils under Pinus halepensis and Pinus sylvestris plantations in Spain:a basis for establishing sustainable forest management strategies.Annals of Forest Science,75(1):34-47
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Chen C,Li Q,Huang WL,Wang J,Fu RT,Luo X,Lu DH,2017.Effects of Morchella white mold disease on soil fungal community structure.Microbiology China,44(11):2652-2659(in Chinese)
Chen C,Li Q,Wang J,Fu RT,Jin X,Xiong C,Lu DH,2018.Effects of morel(Morchella sp.)stipe rot disease occurrence on soil fungal cmmunity structure.Journal of Microbiology,38(5):39-45(in Chinese)
Chen K,Abbott RJ,Milne RI,Tian XM,Liu J,2010.Phylogeography of Pinus tabulaeformis Carr.(Pinaceae),a dominant species of coniferous forest in northern China.Molecular Ecology,17(19):4276-4288
Chen ZB,Gao X,Wang DB,Wang DK,Xu SG,2018.Effects of different biochar application rates on rhizosphere soil microbial diversity of tobacco.Acta Agriculturae Boreali-Sinica,33(1):224-232(in Chinese)
Edgar RC,2013.UPARSE:highly accurate OTU sequences from microbial amplicon reads.Nature Methods,10(10):996
Guo MS,Gao GL,Ding GD,Zhang Y,Zhao YY,Ren Y,2018.Diversity of ectomycorrhizal fungi associated with Pinus sylvestris var.mongolica in Hulunbuir Sandy Land.Mycosystema,37(9):1133-1142(in Chinese)
Guo XW,Li ZY,Qin SJ,Zhou YB,Wei YW,2017.Allocation of soil aggregates,organic carbon and microbial biomass carbon affected by agroforestry modes in Pinus sylvestris var.mongolica plantations in sandy area.Chinese Journal of Ecology,36(11):3036-3042(in Chinese)
Luo WC,Zhao WZ,Sun CP,Yan JL,2018.Changes in species composition,diversity and soil water content of Pinus sylvestris artificial sand-fixation forest along an afforestation successional gradient in Horqin.Journal of Desert Research,38(1):126-132(in Chinese)
Magoc T,Salzberg SL,2011.FLASH:fast length adjustment of short reads to improve genome assemblies.Bioinformatics,27(21):2957-2963
Qi JY,Song RQ,2018.Effects of combined inoculation with Suillus luteus and Trichoderma virens on the roots of Pinus sylvestris var.mongolica in northwest Liaoning Province.Scientia Silvae Sinicae,54(5):62-69(in Chinese)
Sav H,Rafati H,Yasemin?Z,Dalyan-Cilo B,Ener B,Mohammadi F,Ilkit M,Van Diepeningen AD,Seyedmousavi S,2018.Biofilm formation and resistance to fungicides in clinically relevant members of the fungal genus Fusarium.Journal of Fungi,4(1):16
Schloss PD,Westcott SL,Ryabin T,Hall JR,Hartmann M,Hollister EB,Lesniewski RA,Oakley BB,Parks DH,Robinson CJ,Sahl JW,Stres B,Thallinger GG,Van Horn DJ,Weber CF,2009.Introducing mothur:open-source,platform-independent,community-supported software for describing and comparing microbial communities.Applied&Environmental Microbiology,75(23):7537
Tang DL,Tu XL,Fu C,Xiong W,Wang HW,2018.Illumina Miseq Sequencing-based fungal community of rhizosphere soils along root of ramie.Southwest China Journal of Agricultural Sciences,31(10):2160-2164(in Chinese)
Wang Q,Guo LD,2010.Ectomycorrhizal community composition of Pinus tabulaeformis,assessed by ITS-RFLP and ITSsequences.Botany-Botanique,88(6):590-595
Wang SQ,Wu SH,Zhu XM,Ge YX,2018.Research progress in natural regeneration of Pinus sylvestris var.mongolica plantation.World Forestry Research,31(3):58-62(in Chinese)
Wang XB,Wang GS,Liu YS,Chen XS,Shen X,Yin CM,Mao ZQ,2018.Correlation analysis of apple replant disease and soil fungal community structure in the northwest loess plateau area.Acta Horticulturae Sinica,45(5):855-864(in Chinese)
Wu HQ,Sun LL,Liu F,Wang ZY,Cao CW,2018.Preparation of dry flowable formulations of Clonostachys rose by spray drying and application for Sclerotinia sclerotiorum control.Journal of Integrative Agriculture,17(3):613-620
Wu RTGS,Wang J,Zhao RG,Su LGW,2018.Research advances in ectomycorrhizal ecology.Journal of Anhui Agricultural Sciences,46(6):26-28(in Chinese)
Xu FM,Wang CY,Zhu HL,Tang M,2014.Research on root fungal community of Pinus tabuliformis in pine wilt disease damaged areas.Acta Botanica Boreali-Occidentalia Sinica,34(8):1627-1634(in Chinese)
Yang LB,Sui X,Zhu DG,Cui FX,Li JB,Song RQ,Ni HW,2017.Study on fungal communities’characteristics of different Larix gmelini forest types in cold temperate zone.Journal of Central South University of Forestry&Technology,37(12):76-84(in Chinese)
Yin DC,Qi JY,Deng JF,Deng X,2018.Effects of exogenous calcium on ectotrophic mycorrhiza-Pinus sylvestris var.mongolica seedlings and rhizosphere soil.Chinese Journal of Soil Science,49(3):362-366(in Chinese)
Yin DC,Qi JY,Song RQ,2018.The effect of compound inoculation of Suillus luteus and Trichoderma virens on the interannual changes of the bioactivity of Pinus sylvestris var.mongolica rhizosphere.Chinese Journal of Ecology,37(9):2637-2641(in Chinese)
Yin DC,Zhou HM,Gao CY,Wang P,Qin SJ,Xue Y,2017.The biological characteristics research of Suillus luteus.Journal of Shenyang Agricultural University,48(3):348-353(in Chinese)
Zhang H,Yu H,Tang M,2017.Prior contact of Pinus tabulaeformis with ectomycorrhizal fungi increases plant growth and survival from damping-off.New Forests,48(6):855-866
Zhou YJ,Li JH,Zhang GY,Wang N,Tan WL,Wang YP,Wang CY,Wang HF,2018.Analysis of soil fungal diversity and community composition of rubber plantations based on high-throughput sequencing.Journal of Southern Agriculture,49(9):1729-1735(in Chinese)
别格坚巴哈提,邓玉侠,尹大川,宋瑞清,2017.樟子松苗木促生长高效外生菌根菌的筛选.林业科技,42(3):31-33
陈诚,李强,黄文丽,王剑,伏荣桃,罗曦,卢代华,2017.羊肚菌白霉病发生对土壤真菌群落结构的影响.微生物学通报,44(11):2652-2659
陈诚,李强,王剑,伏荣桃,金鑫,熊川,卢代华,2018.羊肚菌烂柄病发生对土壤真菌群落结构的影响.微生物学杂志,38(5):39-45
陈泽斌,高熹,王定斌,王定康,徐胜光,2018.生物炭不同施用量对烟草根际土壤微生物多样性的影响.华北农学报,33(1):224-232
郭米山,高广磊,丁国栋,张英,赵媛媛,任悦,2018.呼伦贝尔沙地樟子松外生菌根真菌多样性.菌物学报,37(9):1133-1142
郭鑫炜,李志远,秦胜金,周永斌,魏亚伟,2017.林农复合对沙地樟子松人工林土壤团聚体、有机碳及微生物生物量碳分配的影响.生态学杂志,36(11):3036-3042
罗维成,赵文智,孙程鹏,闫加亮,2018.科尔沁沙地樟子松(Pinus sylvestris)人工固沙林演变过程中物种多样性和土壤水分特征.中国沙漠,38(1):126-132
祁金玉,宋瑞清,2018.褐环乳牛肝菌与绿木霉复合接种对辽西北地区樟子松根系的影响.林业科学,54(5):62-69
汤涤洛,涂修亮,付聪,熊伟,汪红武,2018.基于高通量测序的苎麻根际土壤真菌群落结构.西南农业学报,31(10):2160-2164
王思琪,武曙红,朱晓梅,葛玉祥,2018.我国樟子松人工林天然更新研究进展.世界林业研究,31(3):58-62
王晓宝,王功帅,刘宇松,陈学森,沈向,尹承苗,毛志泉,2018.西北黄土高原地区苹果连作障碍与土壤真菌群落结构的相关性分析.园艺学报,45(5):855-864
徐风美,王春燕,褚洪龙,唐明,2014.松萎蔫病发生区和未发生区油松根部真菌群落研究.西北植物学报,34(8):1627-1634
乌仁陶格斯,王娟,昭日格,苏楞高娃,2018.外生菌根生态学研究进展.安徽农业科学,46(6):26-28
杨立宾,隋心,朱道光,崔福星,李金博,宋瑞清,倪红伟,2017.大兴安岭兴安落叶松林土壤真菌群落特征研究.中南林业科技大学学报,37(12):76-84
尹大川,祁金玉,邓继峰,邓勋,2018.外源钙添加量对外生菌根-樟子松共生体苗木及根际土壤的影响.土壤通报,49(3):362-366
尹大川,祁金玉,宋瑞清,2018.复合接种褐环乳牛肝菌与绿木霉对樟子松根际土壤生物活性年际变化的影响.生态学杂志,37(9):2637-2641
尹大川,周洪敏,高慈元,王萍,秦世杰,薛莹,2017.褐环乳牛肝菌(Suillus luteus)的生物学特性研究.沈阳农业大学学报,48(3):348-353
周玉杰,李建华,张广宇,王宁,谭文丽,王永鹏,王春燕,王华锋,2018.基于高通量测序的橡胶林土壤真菌多样性及群落组成分析.南方农业学报,49(9):1729-1735
Biegejian B,Deng YX,Yin DC,Song RQ,2017.Screening of ectomycorrhizal fungi for promoting the growth of seedlings of Pinus sylvestris var.mongolica.Forestry Science&Technology,42(3):31-33(in Chinese)
Bueis T,Turrión MB,Bravo F,Pando V,Muscolo A,2018.Factors determining enzyme activities in soils under Pinus halepensis and Pinus sylvestris plantations in Spain:a basis for establishing sustainable forest management strategies.Annals of Forest Science,75(1):34-47
Caporaso JG,Kuczynski J,Stombaugh J,Bittinger K,Bushman FD,Costello EK,Fierer N,Pena AG,Goodrich JK,Gordon JI,Huttley GA,Kelley ST,Knights D,Koenig JE,Ley RE,Lozupone CA,McDonald D,Muegge BD,Pirrung M,Reeder J,Sevinsky JR,Tumbaugh PJ,Walters WA,Widmann J,Yatsunenko T,Zaneveld J,Knight R,2010.QIIME allows analysis of high-throughput community sequencing data.Nature Methods,7(5):335-336
Chen C,Li Q,Huang WL,Wang J,Fu RT,Luo X,Lu DH,2017.Effects of Morchella white mold disease on soil fungal community structure.Microbiology China,44(11):2652-2659(in Chinese)
Chen C,Li Q,Wang J,Fu RT,Jin X,Xiong C,Lu DH,2018.Effects of morel(Morchella sp.)stipe rot disease occurrence on soil fungal cmmunity structure.Journal of Microbiology,38(5):39-45(in Chinese)
Chen K,Abbott RJ,Milne RI,Tian XM,Liu J,2010.Phylogeography of Pinus tabulaeformis Carr.(Pinaceae),a dominant species of coniferous forest in northern China.Molecular Ecology,17(19):4276-4288
Chen ZB,Gao X,Wang DB,Wang DK,Xu SG,2018.Effects of different biochar application rates on rhizosphere soil microbial diversity of tobacco.Acta Agriculturae Boreali-Sinica,33(1):224-232(in Chinese)
Edgar RC,2013.UPARSE:highly accurate OTU sequences from microbial amplicon reads.Nature Methods,10(10):996
Guo MS,Gao GL,Ding GD,Zhang Y,Zhao YY,Ren Y,2018.Diversity of ectomycorrhizal fungi associated with Pinus sylvestris var.mongolica in Hulunbuir Sandy Land.Mycosystema,37(9):1133-1142(in Chinese)
Guo XW,Li ZY,Qin SJ,Zhou YB,Wei YW,2017.Allocation of soil aggregates,organic carbon and microbial biomass carbon affected by agroforestry modes in Pinus sylvestris var.mongolica plantations in sandy area.Chinese Journal of Ecology,36(11):3036-3042(in Chinese)
Luo WC,Zhao WZ,Sun CP,Yan JL,2018.Changes in species composition,diversity and soil water content of Pinus sylvestris artificial sand-fixation forest along an afforestation successional gradient in Horqin.Journal of Desert Research,38(1):126-132(in Chinese)
Magoc T,Salzberg SL,2011.FLASH:fast length adjustment of short reads to improve genome assemblies.Bioinformatics,27(21):2957-2963
Qi JY,Song RQ,2018.Effects of combined inoculation with Suillus luteus and Trichoderma virens on the roots of Pinus sylvestris var.mongolica in northwest Liaoning Province.Scientia Silvae Sinicae,54(5):62-69(in Chinese)
Sav H,Rafati H,Yasemin?Z,Dalyan-Cilo B,Ener B,Mohammadi F,Ilkit M,Van Diepeningen AD,Seyedmousavi S,2018.Biofilm formation and resistance to fungicides in clinically relevant members of the fungal genus Fusarium.Journal of Fungi,4(1):16
Schloss PD,Westcott SL,Ryabin T,Hall JR,Hartmann M,Hollister EB,Lesniewski RA,Oakley BB,Parks DH,Robinson CJ,Sahl JW,Stres B,Thallinger GG,Van Horn DJ,Weber CF,2009.Introducing mothur:open-source,platform-independent,community-supported software for describing and comparing microbial communities.Applied&Environmental Microbiology,75(23):7537
Tang DL,Tu XL,Fu C,Xiong W,Wang HW,2018.Illumina Miseq Sequencing-based fungal community of rhizosphere soils along root of ramie.Southwest China Journal of Agricultural Sciences,31(10):2160-2164(in Chinese)
Wang Q,Guo LD,2010.Ectomycorrhizal community composition of Pinus tabulaeformis,assessed by ITS-RFLP and ITSsequences.Botany-Botanique,88(6):590-595
Wang SQ,Wu SH,Zhu XM,Ge YX,2018.Research progress in natural regeneration of Pinus sylvestris var.mongolica plantation.World Forestry Research,31(3):58-62(in Chinese)
Wang XB,Wang GS,Liu YS,Chen XS,Shen X,Yin CM,Mao ZQ,2018.Correlation analysis of apple replant disease and soil fungal community structure in the northwest loess plateau area.Acta Horticulturae Sinica,45(5):855-864(in Chinese)
Wu HQ,Sun LL,Liu F,Wang ZY,Cao CW,2018.Preparation of dry flowable formulations of Clonostachys rose by spray drying and application for Sclerotinia sclerotiorum control.Journal of Integrative Agriculture,17(3):613-620
Wu RTGS,Wang J,Zhao RG,Su LGW,2018.Research advances in ectomycorrhizal ecology.Journal of Anhui Agricultural Sciences,46(6):26-28(in Chinese)
Xu FM,Wang CY,Zhu HL,Tang M,2014.Research on root fungal community of Pinus tabuliformis in pine wilt disease damaged areas.Acta Botanica Boreali-Occidentalia Sinica,34(8):1627-1634(in Chinese)
Yang LB,Sui X,Zhu DG,Cui FX,Li JB,Song RQ,Ni HW,2017.Study on fungal communities’characteristics of different Larix gmelini forest types in cold temperate zone.Journal of Central South University of Forestry&Technology,37(12):76-84(in Chinese)
Yin DC,Qi JY,Deng JF,Deng X,2018.Effects of exogenous calcium on ectotrophic mycorrhiza-Pinus sylvestris var.mongolica seedlings and rhizosphere soil.Chinese Journal of Soil Science,49(3):362-366(in Chinese)
Yin DC,Qi JY,Song RQ,2018.The effect of compound inoculation of Suillus luteus and Trichoderma virens on the interannual changes of the bioactivity of Pinus sylvestris var.mongolica rhizosphere.Chinese Journal of Ecology,37(9):2637-2641(in Chinese)
Yin DC,Zhou HM,Gao CY,Wang P,Qin SJ,Xue Y,2017.The biological characteristics research of Suillus luteus.Journal of Shenyang Agricultural University,48(3):348-353(in Chinese)
Zhang H,Yu H,Tang M,2017.Prior contact of Pinus tabulaeformis with ectomycorrhizal fungi increases plant growth and survival from damping-off.New Forests,48(6):855-866
Zhou YJ,Li JH,Zhang GY,Wang N,Tan WL,Wang YP,Wang CY,Wang HF,2018.Analysis of soil fungal diversity and community composition of rubber plantations based on high-throughput sequencing.Journal of Southern Agriculture,49(9):1729-1735(in Chinese)
别格坚巴哈提,邓玉侠,尹大川,宋瑞清,2017.樟子松苗木促生长高效外生菌根菌的筛选.林业科技,42(3):31-33
陈诚,李强,黄文丽,王剑,伏荣桃,罗曦,卢代华,2017.羊肚菌白霉病发生对土壤真菌群落结构的影响.微生物学通报,44(11):2652-2659
陈诚,李强,王剑,伏荣桃,金鑫,熊川,卢代华,2018.羊肚菌烂柄病发生对土壤真菌群落结构的影响.微生物学杂志,38(5):39-45
陈泽斌,高熹,王定斌,王定康,徐胜光,2018.生物炭不同施用量对烟草根际土壤微生物多样性的影响.华北农学报,33(1):224-232
郭米山,高广磊,丁国栋,张英,赵媛媛,任悦,2018.呼伦贝尔沙地樟子松外生菌根真菌多样性.菌物学报,37(9):1133-1142
郭鑫炜,李志远,秦胜金,周永斌,魏亚伟,2017.林农复合对沙地樟子松人工林土壤团聚体、有机碳及微生物生物量碳分配的影响.生态学杂志,36(11):3036-3042
罗维成,赵文智,孙程鹏,闫加亮,2018.科尔沁沙地樟子松(Pinus sylvestris)人工固沙林演变过程中物种多样性和土壤水分特征.中国沙漠,38(1):126-132
祁金玉,宋瑞清,2018.褐环乳牛肝菌与绿木霉复合接种对辽西北地区樟子松根系的影响.林业科学,54(5):62-69
汤涤洛,涂修亮,付聪,熊伟,汪红武,2018.基于高通量测序的苎麻根际土壤真菌群落结构.西南农业学报,31(10):2160-2164
王思琪,武曙红,朱晓梅,葛玉祥,2018.我国樟子松人工林天然更新研究进展.世界林业研究,31(3):58-62
王晓宝,王功帅,刘宇松,陈学森,沈向,尹承苗,毛志泉,2018.西北黄土高原地区苹果连作障碍与土壤真菌群落结构的相关性分析.园艺学报,45(5):855-864
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