桑寄生繁殖技术研究
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摘要
本文从接种方法、时间、部位及接种后的栽培管理等方面对桑寄生Taxillus chinensis(DC.)Danser的繁殖技术进行比较系统的研究,旨在为将来的桑寄生栽培提供理论依据和技术指导。试验结果表明,不同接种部位、接种时间、接种方法及管理措施对桑寄生的萌芽率、寄生率有重要的影响;模拟桑寄生野生繁殖状态,3月份将种子与鸟粪以1∶5的比例拌匀后接种到桑树上部枝条并进行人工管理的萌发率和寄生率最高。本文建立了一种高效的桑寄生繁殖方法。
In this paper,in order to provide theoretical basis and technical guidance for the future parasitic cultivation of the Taxillus chinensis(DC.) Danser,the breeding techniques of marigold parasitic Taxillus chinensis(DC.) Danser were systematically studied from the inoculation method,time,sites and cultivation management after inoculation. The test results showed that different inoculation sites,inoculation time,inoculation methods and management measures had important effects on the germination rate and parasitism rate of the Taxillus chinensis(DC.) Danser. In March,guano and seeds were mixed at a ratio of 1∶5,and then inoculated into the upper branches of mulberry trees and artificially managed,so that the germination rate and parasitism rate were the highest. An efficient method for breeding mulberry parasite was established in this article.
In this paper,in order to provide theoretical basis and technical guidance for the future parasitic cultivation of the Taxillus chinensis(DC.) Danser,the breeding techniques of marigold parasitic Taxillus chinensis(DC.) Danser were systematically studied from the inoculation method,time,sites and cultivation management after inoculation. The test results showed that different inoculation sites,inoculation time,inoculation methods and management measures had important effects on the germination rate and parasitism rate of the Taxillus chinensis(DC.) Danser. In March,guano and seeds were mixed at a ratio of 1∶5,and then inoculated into the upper branches of mulberry trees and artificially managed,so that the germination rate and parasitism rate were the highest. An efficient method for breeding mulberry parasite was established in this article.
引文
[1]国家药典委员会.中华人民共和国药典:2015年版一部[M].北京:中国医药科技出版社,2015.
[2]PRITCHARD H W.Water potential and embryonic axis viability in recalcitrant seeds of quercus rubra[J].Annals of Botany,1991,67:43-49.
[3]DING B,DAI Y,HOU Y L,et al.Four new hemiterpenoid derivatives from Taxillus chinensis[J].Fitoterapia,2013,86:1-5.
[4]李永华,陈士林,卢栋,等.不同寄主植物桑寄生总黄酮含量研究[J].时珍国医国药,2009,20(12):3009-3010.
[5]张协君,朱开昕,赵明惠,等.不同寄主来源的桑寄生药材槲皮苷与槲皮素含量分析[J].时珍国医国药,2011,22(7):1604-1606.
[6]叶立新,王继红,黄华利.桑寄生对肾性高血压大鼠血浆β-内啡肽浓度影响的量效作用[J].中国临床康复,2005,9(27):84-85.
[7]肖义军,刘奋,陈元仲.红花桑寄生总黄酮提取物诱导淋巴瘤细胞株CA46凋亡及其分子机制研究[J].天然产物研究与开发,2008,20:797-802.
[8]龙启才,邱建波.威灵仙、秦艽、桑寄生醇提物体外对淋巴细胞和环氧酶的影响[J].中药药理与临床,2004,20(4):26-27.
[9]刘立章.对中药桑寄生治病方剂与药膳的临床分析[J].当代医药论丛,2004,12(16):16.
[10]鲁长虎.槲寄生的生物学特征及鸟类对其种子的传播[J].生态学报,2003,23(4):834-839.
[11]李永华,阮金兰,陈士林,等.广寄生种子结构及其萌发实验研究[J].世界科学技术:中医药现代化,2010,12(6):920-923.
[12]李永华,卢栋,朱开昕,等.桑寄生野生资源与规范化种植技术[J].广西中医药,2010,33(1):53-55.
[13]BERJAK P,PAMMENTER N W.Progress in the understanding and manipulation of desiccation-sensitivity(recalcitrant)seeds[M]//ELLIS R H,BLACK M,MUR-DOCH A J,et al.Basic and applied aspects of seed biology.Dordrecht:Kluwer Academic Publishers,1997:689-703.
[14]韦树根,付金娥,潘丽梅,等.桑寄生顽拗性种子发芽影响因素研究[J].北方园艺,2017(14):155-159.
[15]潘丽梅,马小军,付金娥,等.桑寄生种子质量分级标准研究[J].北方园艺,2017(20):144-148.
[2]PRITCHARD H W.Water potential and embryonic axis viability in recalcitrant seeds of quercus rubra[J].Annals of Botany,1991,67:43-49.
[3]DING B,DAI Y,HOU Y L,et al.Four new hemiterpenoid derivatives from Taxillus chinensis[J].Fitoterapia,2013,86:1-5.
[4]李永华,陈士林,卢栋,等.不同寄主植物桑寄生总黄酮含量研究[J].时珍国医国药,2009,20(12):3009-3010.
[5]张协君,朱开昕,赵明惠,等.不同寄主来源的桑寄生药材槲皮苷与槲皮素含量分析[J].时珍国医国药,2011,22(7):1604-1606.
[6]叶立新,王继红,黄华利.桑寄生对肾性高血压大鼠血浆β-内啡肽浓度影响的量效作用[J].中国临床康复,2005,9(27):84-85.
[7]肖义军,刘奋,陈元仲.红花桑寄生总黄酮提取物诱导淋巴瘤细胞株CA46凋亡及其分子机制研究[J].天然产物研究与开发,2008,20:797-802.
[8]龙启才,邱建波.威灵仙、秦艽、桑寄生醇提物体外对淋巴细胞和环氧酶的影响[J].中药药理与临床,2004,20(4):26-27.
[9]刘立章.对中药桑寄生治病方剂与药膳的临床分析[J].当代医药论丛,2004,12(16):16.
[10]鲁长虎.槲寄生的生物学特征及鸟类对其种子的传播[J].生态学报,2003,23(4):834-839.
[11]李永华,阮金兰,陈士林,等.广寄生种子结构及其萌发实验研究[J].世界科学技术:中医药现代化,2010,12(6):920-923.
[12]李永华,卢栋,朱开昕,等.桑寄生野生资源与规范化种植技术[J].广西中医药,2010,33(1):53-55.
[13]BERJAK P,PAMMENTER N W.Progress in the understanding and manipulation of desiccation-sensitivity(recalcitrant)seeds[M]//ELLIS R H,BLACK M,MUR-DOCH A J,et al.Basic and applied aspects of seed biology.Dordrecht:Kluwer Academic Publishers,1997:689-703.
[14]韦树根,付金娥,潘丽梅,等.桑寄生顽拗性种子发芽影响因素研究[J].北方园艺,2017(14):155-159.
[15]潘丽梅,马小军,付金娥,等.桑寄生种子质量分级标准研究[J].北方园艺,2017(20):144-148.