利用谷氨酸离交尾液发酵生产普鲁兰的研究
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摘要
对味精生产过程中产生的离交尾液进行了成分分析,尝试采用味精生产过程中产生的离交尾液作为发酵基质生产普鲁兰。培养条件为调整初始pH值到6.5,以500ml摇瓶装150ml的装液量,按10%的接种量接种,发酵温度28℃,200r/min摇床发酵培养7天。可得到粗多糖23.5g/L,多糖的转化率为47%,经活性碳过柱去色素后,再去除杂蛋白可得到普鲁兰多糖,回收率为65%。
The component of ion- exchange wastewater which can be produced in the process of manufacturing monosodium glutamate was analyzed,and the ion-exchange wastewater was used as the fermentation matrix to produce pullulan.The optimum conditions of culture are as follows:initial pH is 6.5,pullulan cells are transferred to 500ml conical flasks containing 150ml culture medium;inoculation is incubated according to 10%dosage;The culture medium is carried out in a rotary shaker incubator at 200r/min and at 28℃ for 7 days.As a result,23.5g /L Crude pullulan can be obtained,and the conversion rate is 47%.By shucking off pigment through activated carbon column and then removing protein,the recovery rate of pullulan reaches 65%.
The component of ion- exchange wastewater which can be produced in the process of manufacturing monosodium glutamate was analyzed,and the ion-exchange wastewater was used as the fermentation matrix to produce pullulan.The optimum conditions of culture are as follows:initial pH is 6.5,pullulan cells are transferred to 500ml conical flasks containing 150ml culture medium;inoculation is incubated according to 10%dosage;The culture medium is carried out in a rotary shaker incubator at 200r/min and at 28℃ for 7 days.As a result,23.5g /L Crude pullulan can be obtained,and the conversion rate is 47%.By shucking off pigment through activated carbon column and then removing protein,the recovery rate of pullulan reaches 65%.
引文
[1]Madlova A,Antosova M,Polakovic M,et al.Thermal stability of fructosyl-transferase from aureobaiaosidium pullulans.Chem Pap,2000,54(6a):339-344
[2]A LeDuy,Boa J M.Pullulan production from peat hydrolyzate.Can J Microbiol,1982,29:143-146
[3]Israilides C,Scanlon B,Smith A,et al.Characterization of pullulans produced from agro-industrial wastes.Carbohydr Pol,1994,25:203-209
[4]Israilides C J,Smith A,Harthill J E,et al.Pullulan content of the ethanol precipitate from fermented agroindustrial wastes.Appl Microbiol Biotechnol,1998,49:613-617
[5]Roukas T.Pretreatment of beet molasses to increase pullulan production.Process Biochem,1998,33:805-810
[6]殷燕芳,彭少贤.茁霉多糖结构分析和应用.湖北工学院学报,1999,14(1-2):25-28Yin Y F,Peng S X.Journal of Hubei Polytechnic University,1999,14(1-2):25-28
[7]陈洁,傅正生,达文燕,等.土豆淀粉废水发酵普鲁兰多糖的研究.农业与技术,2006,26(2):112-114Chen J,Fu Z S,Da W Y,et al.Agriculture&Technology,2006,26(1):112-114
[8]常天俊.出芽短梗霉多糖发酵工艺的研究.华中科技大学,2004.17-18Chang T J.Huanzhong University of Science and Technology,2004,17-18
[9]李如亮.生物化学实验.武汉:武汉大学出版社,1998.9-10Li R L.Biochemistry Experiment.Wuhan:Wuhan University Publishing house,1998.9-10
[2]A LeDuy,Boa J M.Pullulan production from peat hydrolyzate.Can J Microbiol,1982,29:143-146
[3]Israilides C,Scanlon B,Smith A,et al.Characterization of pullulans produced from agro-industrial wastes.Carbohydr Pol,1994,25:203-209
[4]Israilides C J,Smith A,Harthill J E,et al.Pullulan content of the ethanol precipitate from fermented agroindustrial wastes.Appl Microbiol Biotechnol,1998,49:613-617
[5]Roukas T.Pretreatment of beet molasses to increase pullulan production.Process Biochem,1998,33:805-810
[6]殷燕芳,彭少贤.茁霉多糖结构分析和应用.湖北工学院学报,1999,14(1-2):25-28Yin Y F,Peng S X.Journal of Hubei Polytechnic University,1999,14(1-2):25-28
[7]陈洁,傅正生,达文燕,等.土豆淀粉废水发酵普鲁兰多糖的研究.农业与技术,2006,26(2):112-114Chen J,Fu Z S,Da W Y,et al.Agriculture&Technology,2006,26(1):112-114
[8]常天俊.出芽短梗霉多糖发酵工艺的研究.华中科技大学,2004.17-18Chang T J.Huanzhong University of Science and Technology,2004,17-18
[9]李如亮.生物化学实验.武汉:武汉大学出版社,1998.9-10Li R L.Biochemistry Experiment.Wuhan:Wuhan University Publishing house,1998.9-10