基于阿基米德螺旋线圈的高变化率脉冲磁场发生器
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摘要
脉冲磁场治疗肿瘤凭借其无创性的优势具有良好的应用前景。感应电场和感应电流是脉冲磁场产生生物效应的原因之一。为了获得较大的感应电场来开展脉冲磁场治疗肿瘤的细胞实验,研制了1台基于阿基米德螺旋线圈和自击穿空气开关的高变化率脉冲磁场发生器。对阿基米德螺旋线圈周围空间的磁场和感应电场进行了计算,利用COMSOL Multiphysics软件对线圈的磁场分布特性进行了优化设计,并对研制出的磁场发生器性能进行了实测。最终,设计的阿基米德螺旋线圈为7匝、外径为21 mm,该线圈在细胞培养液中可产生的脉冲磁场参数为:磁感应强度幅值0.92 T、上升时间78 ns、脉冲宽度1.8μs,磁场的变化率达1.1×107 T/s,感应电场最大值为55.9kV/m,电场的均匀度达84%,最大的感应电流密度达1.7×104 A/m2。结果表明,此脉冲磁场发生器可以满足用于研究脉冲磁场治疗肿瘤相关的细胞实验的要求,为后续研究奠定了基础。
Pulsed magnetic field treatment with cancer has good application prospects depending on its advantages of non-invasive. One of the mechanisms for pulsed magnetic field causing biological effects is from the induced electric field and magnetic field induced current. In order to obtain a larger induced electric field to carry out the cell experiments of pulsed magnetic field treatment on tumor cells, we develop a pulsed magnetic field generator with a high-variation rate by Archimedes spiral coil and the self-breakdown air switch. And we calculate the magnetic field and the induced electric field around the Archimedes spiral coil. By using COMSOL Multiphysics software, magnetic field distribution characteristic of coil is optimizing designed. Then the performance of the generator is measured. Finally, the Archimedean spiral coil has 7 turns, and the outer diameter is 21 mm. The pulsed magnetic field parameters can be produced in the cell culture medium as follows: the magnetic induction magnitude of 0.92 T, the rise time of 78 ns, the pulse width of 1.8 μs. And rate of change of magnetic field reaches 1.1×107 T/s, induced electric field maximum is 55.9 kV/m, electric field uniform degree can achieve 84%, and the maximum of the induced current density reaches 1.7×104 A/m2. The results show that the pulsed magnetic field generator can meet the requirements of the experiment cells associated with pulsed magnetic therapy treating with tumors, which lay the foundation for future study.
Pulsed magnetic field treatment with cancer has good application prospects depending on its advantages of non-invasive. One of the mechanisms for pulsed magnetic field causing biological effects is from the induced electric field and magnetic field induced current. In order to obtain a larger induced electric field to carry out the cell experiments of pulsed magnetic field treatment on tumor cells, we develop a pulsed magnetic field generator with a high-variation rate by Archimedes spiral coil and the self-breakdown air switch. And we calculate the magnetic field and the induced electric field around the Archimedes spiral coil. By using COMSOL Multiphysics software, magnetic field distribution characteristic of coil is optimizing designed. Then the performance of the generator is measured. Finally, the Archimedean spiral coil has 7 turns, and the outer diameter is 21 mm. The pulsed magnetic field parameters can be produced in the cell culture medium as follows: the magnetic induction magnitude of 0.92 T, the rise time of 78 ns, the pulse width of 1.8 μs. And rate of change of magnetic field reaches 1.1×107 T/s, induced electric field maximum is 55.9 kV/m, electric field uniform degree can achieve 84%, and the maximum of the induced current density reaches 1.7×104 A/m2. The results show that the pulsed magnetic field generator can meet the requirements of the experiment cells associated with pulsed magnetic therapy treating with tumors, which lay the foundation for future study.
引文
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[18]米彦,储贻道,蒋春,等.基于聚焦线圈和固态开关的脉冲磁场发生器[J].仪器仪表学报,2014,35(7):1639-1645.MI Yan,CHU Yidao,JIANG Chun,et al.Pulsed magnetic field generator based on spatially focusing coil array and solid state switch[J].Chinese Journal of Scientific Instrument,2014,35(7):1639-1645.
[19]SCHOENBACH K H,HATSUKI S,STARK R H,et al.Bioelectrics-new applications for pulsed power technology[J].IEEE Transactions on Plasma Science,2002,30(1):293-300.
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[21]王剑飞.多参数可调纳秒脉冲发生器的研制及其诱导的生物医学效应研究[D].重庆:重庆大学,2010:22-26.WANG Jianfei.Development of multi-parameter adjustable nanosecond pulse generator and its biomedical effects research[D].Chongqing,China:Chongqing University,2010:22-26.
[22]Martin T,Guenther A,Kristiansen M.JC martin on pulsed power[M].New York,USA:Plenum Press,1996:297-299.
[23]姚陈果,莫登斌,孙才新,等.细胞电参数对内外膜跨膜电位影响的仿真研究[J].中国生物医学工程学报,2007,26(5):739-745.YAO Chenguo,MO Dengbin,SUN Caixin,et al.Simulation of transmembrane potentials on inner and outer membrane under different cellular electrical parameters[J].Chinese Journal of Biomedical Engineering,2007,26(5):739-745.
[2]董守龙,姚陈果,储贻道,等.不可逆电穿孔对兔组织阻抗的影响[J].高电压技术,2015,41(4):1402-1408.DONG Shoulong,YAO Chenguo,CHU Yidao,et al.Influence of irreversible electroporation on tissues resistance of rabbit[J].High Voltage Engineering,2015,41(4):1402-1408.
[3]李成祥,张睿哲,姚陈果,等.皮秒脉冲细胞实验装置的设计与仿真优化[J].高电压技术,2015,41(4):1365-1371.LI Chengxiang,ZHANG Ruizhe,YAO Chenguo,et al.Design and simulation optimization of picosecond pulsed cell experimental device[J].High Voltage Engineering,2015,41(4):1365-1371.
[4]米彦,储贻道,谭冲,等.毫秒脉冲磁场作用下细胞内带电粒子的受力分析[J].高电压技术,2015,41(4):1357-1364.MI Yan,CHU Yidao,TAN Chong,et al.Stress analysis of charged particles in cell under millisecond pulsed magnetic field[J].High Voltage Engineering,2015,41(4):1357-1364.
[5]DE SEZE R,BOUTHET C,TUFFET S,et al.Effects of time-varying uniform magnetic fields on natural killer cell activity and antibody response in mice[J].Bioelectromagnetics,1993,14(5):405-12
[6]TUFFET S,DE SEZE R,MOREAU J M,et al.Effects of a strong pulsed magnetic field on the proliferation of tumour cells in vitro[J].Bioelectrochemistry and Bioenergetics,1993,30(1):151-160.
[7]DE SEZE R,TUFFET S,MOREAU J M,et al.Effects of 100 m T time varying magnetic fields on the growth of tumors in mice[J].Bioelectromagnetics,2000,21(2):107-111.
[8]姚学玲,徐晓宙,陈景亮,等.脉冲电流磁场对鼠骨髓瘤细胞的影响[J].西安交通大学学报,2002,36(2):134-137.YAO Xueling,XU Xiaozhou,CHEN Jingliang,et al.Effect of impulse current magnetic field on the mouse marrow tumor cells[J].Journal of Xi’an Jiao Tong University,2002,36(2):134-137.
[9]胡大为,王群,范志民.脉冲强磁场对小鼠Louis肺癌杀伤作用的形态学研究[J].中华物理医学与康复杂志,1998,20(2):133-135.HU Dawei,WANG Qun,FAN Zhimin.Morphological study of injurious effect of strong impulse magnetic field on Louis lung cancer in mice[J].Chinese Journal of Physical Medical,1998,20(2):133-135.
[10]NOVICKIJ V,GRAINYS A,NOVICKIJ J,et al.Compact microsecond pulsed magnetic field generator for application in bioelectronics[J].Elektronika Irelektrotechnika,2013,19(8):25-28.
[11]DINIZ P,SOEJIMA K,ITO G.Nitric oxide mediates the effects of pulsed electromagnetic field stimulation on the osteoblast proliferation and differentiation[J].Nitric Oxide,2002,7(1):18-23.
[12]VENTURA C,MAIOLI M,PINTUS G,et al.Elf-pulsed magnetic fields modulate opioid peptide gene expression in myocardial cells[J].Cardiovascular Research,2000,45(4):1054-1064.
[13]DE SEZE R,BOUTHET C,TUFFET S,et al.Effects of time-varying uniform magnetic fields on natural killer cell activity and antibody response in mice[J].Bioelectromagnetic,1993,14(5):405-412.
[14]SEZE R D,TUFFET S,MOREAU J M,et al.Effects of 100 m T time varying magnetic fields on the growth of tumors in mice[J].Bioelectromagnetic,2000,21(1):107-111.
[15]MI Y,YAO C,JIANG C,et al.A ns-μs duration,millitesla,exponential decay pulsed magnetic fields generator for tumor treatment[J].IEEE Transactions on Dielectrics&Electrical Insulation,2011,18(4):1111-1118.
[16]席晓莉,魏明,文峻.CXLY-Ⅲ型低频脉冲磁场发生仪的研制[J].生物医学工程学杂志,1999,16(3):314-317.XI Xiaoli,WEI Ming,WEN Jun.Design of CXLY-Ⅱlow frequency magnetic fields pulse generator[J].Journal of Biomedical Engineering,1999,16(3):314-317.
[17]张沪生,邓仁清.超低频脉冲磁场诱导癌细胞凋亡机理研究[J].现代生物医学进展,2006,6(2):13-15.ZHANG Husheng,DENG Renqing.Mechanism studies on ultralow frequency pulsed magnetic field inducing apoptosis of cancer cell[J].Progress in Modern Biomedicine,2006,6(2):13-15.
[18]米彦,储贻道,蒋春,等.基于聚焦线圈和固态开关的脉冲磁场发生器[J].仪器仪表学报,2014,35(7):1639-1645.MI Yan,CHU Yidao,JIANG Chun,et al.Pulsed magnetic field generator based on spatially focusing coil array and solid state switch[J].Chinese Journal of Scientific Instrument,2014,35(7):1639-1645.
[19]SCHOENBACH K H,HATSUKI S,STARK R H,et al.Bioelectrics-new applications for pulsed power technology[J].IEEE Transactions on Plasma Science,2002,30(1):293-300.
[20]张广浩,刘兴发,魏树峰,等.用于动物长期实验的大型工频磁场曝露系统[J].高电压技术,2013,39(1):149-155.ZHANG Guanghao,LIU Xingfa,WEI Shufeng,et al.Large power frequency magnetic field exposure system for long term animal experiments[J].High Voltage Engineering,2013,39(1):149-155.
[21]王剑飞.多参数可调纳秒脉冲发生器的研制及其诱导的生物医学效应研究[D].重庆:重庆大学,2010:22-26.WANG Jianfei.Development of multi-parameter adjustable nanosecond pulse generator and its biomedical effects research[D].Chongqing,China:Chongqing University,2010:22-26.
[22]Martin T,Guenther A,Kristiansen M.JC martin on pulsed power[M].New York,USA:Plenum Press,1996:297-299.
[23]姚陈果,莫登斌,孙才新,等.细胞电参数对内外膜跨膜电位影响的仿真研究[J].中国生物医学工程学报,2007,26(5):739-745.YAO Chenguo,MO Dengbin,SUN Caixin,et al.Simulation of transmembrane potentials on inner and outer membrane under different cellular electrical parameters[J].Chinese Journal of Biomedical Engineering,2007,26(5):739-745.