不同因子对纤维素降解真菌分离效率的影响
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摘要
研究土样处理方法、土壤基质类型、土样稀释倍数(10~(-1)、10~(-2)、10~(-3)、10~(-4))等因子对纤维素降解真菌分离率的影响,得到提高纤维素降解真菌分离率的最佳方案。以西藏林芝的腐殖质土、沙质土为实验材料,采用稀释分离法分离纤维素降解真菌,统计不同处理下纤维素降解真菌的分离率。研究发现,将土样阴干后并研细可提高纤维素降解真菌的分离率;腐殖质土及100倍土壤稀释液分离效果好;碳源为CMC-Na,氮源为混合氮源的分离培养基分离率最佳;菌株培养温度为25℃时纤维素降解真菌的分离率最高。本试验为高效分离纤维素降解真菌提供了理论依据。
The influence of soil sample treatment method, soil matrix type, soil sample dilution times(10~(-1), 10~(-2), 10~(-3), 10~(-4)) and other factors on the separation rate of cellulose-degrading fungi were studied in this paper in order to get the best scheme to improve the separation rate of cellulose-degrading fungi. Taking the humus soil and sandy soil of Nyingchi in Tibet as experimental materials, cellulose-degrading fungi were separated by dilution separation method, and the separation rates of cellulose-degrading fungi under different treatments were statistically analyzed. It was found that drying soil samples in the shade and grinding improved the separation rates of cellulose-degrading fungi. Humus soil and 100 times soil diluent had good separation effects. The separation medium with CMC-Na as carbon source and mixed nitrogen source as nitrogen source had the best separation rate. The isolation rate of cellulose-degrading fungi was the highest when the strain culture temperature was 25℃.This experiment provided a theoretical basis for the efficient separation of cellulose-degrading fungi.
The influence of soil sample treatment method, soil matrix type, soil sample dilution times(10~(-1), 10~(-2), 10~(-3), 10~(-4)) and other factors on the separation rate of cellulose-degrading fungi were studied in this paper in order to get the best scheme to improve the separation rate of cellulose-degrading fungi. Taking the humus soil and sandy soil of Nyingchi in Tibet as experimental materials, cellulose-degrading fungi were separated by dilution separation method, and the separation rates of cellulose-degrading fungi under different treatments were statistically analyzed. It was found that drying soil samples in the shade and grinding improved the separation rates of cellulose-degrading fungi. Humus soil and 100 times soil diluent had good separation effects. The separation medium with CMC-Na as carbon source and mixed nitrogen source as nitrogen source had the best separation rate. The isolation rate of cellulose-degrading fungi was the highest when the strain culture temperature was 25℃.This experiment provided a theoretical basis for the efficient separation of cellulose-degrading fungi.
引文
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[16]白宝伟,张琴,滕立平,等.一株棉秆纤维素分解真菌的分离筛选及酶学性质研究[J].新疆农业科学,2012,49(04):701-707.
[2]颜霞,秦魏.降解纤维素真菌的分离筛选及其环境适应性初探[J].中国农学通报,2008(05):44-49.
[3]刘胜贵,严明,邹娟,等.纤维素降解真菌的筛选及其产酶条件[J].中国农学通报,2011,27(07):102-106.
[4]周俊强,邱忠平,韩云平,等.纤维素降解菌的筛选及其产酶特性[J].环境工程学报,2010,4(03):705-708.
[5]许凤华,翟珊珊,刘亭亭,等.高效纤维素降解真菌的筛选及粗酶活性[J].安徽农业科学,2015,43(21):7-10+17.
[6]邹潇潇,易子霆,孙前光,等.纤维素降解真菌DF14101的筛选与鉴定[J].微生物学杂志,2016,36(06):68-72.
[7]金伟,陈文静,姚斌,等.一株产纤维素酶的耐热烟曲霉筛选及产酶条件研究[J].中国酿造,2012,31(06):61-64.
[8]曾青兰,洪玉枝,刘子铎.纤维素降解菌Gibberella fujikuroi产酶条件的优化[J].华中农业大学学报,2008(03):391-393.
[9]刘淑霞,吴海燕,吴海文,等.黑土玉米田土壤纤维素分解真菌物种鉴定及分解能力测定[J].玉米科学,2008(03):112-116.
[10]孔雅丽.东祁连山高寒草甸分解纤维素的真菌多样性及其降解特性[D].甘肃农业大学,2014.
[11]陈静,匡成兵.纤维素降解真菌的分离筛选[J].西南大学学报(自然科学版),2016,38(08):22-26.
[12]叶姜瑜.纤维素-刚果红培养基对土壤纤维分解菌的直接计数[J].西南师范大学学报(自然科学版),1996(06):95-99.
[13]张宇昊,王颉,张伟,等.一种改进的纤维素分解菌鉴别培养基[J].纤维素科学与技术,2004(01):33-36.
[14]黄宁珍,付传明,何金祥,等.纤维素降解真菌分离筛选、产酶特性及高效水解菌系的初步构建[J].西南农业学报,2010,23(05):1489-1496.
[15]勾长龙,王雨琼,王巍,等.两株长白山地区低温纤维素降解真菌的筛选鉴定及其产酶条件优化[J].中山大学学报(自然科学版),2015,54(05):115-121+129.
[16]白宝伟,张琴,滕立平,等.一株棉秆纤维素分解真菌的分离筛选及酶学性质研究[J].新疆农业科学,2012,49(04):701-707.