12份扁蓿豆种质苗期耐盐性综合评价
|
摘要
利用不同浓度NaCl(0,100,200,300mmol·L-1)溶液处理12份扁蓿豆(Medicago ruthenica)种质种子,通过砂培法研究其苗期的耐盐性。结果表明,随着NaCl浓度的增加,供试12份扁蓿豆种质的绝对株高、单株地上生物量和叶片相对含水量逐渐降低,可溶性糖含量不断上升。与对照相比,景泰种质绝对株高平均降幅最大,为16.4%;镇原种质绝对株高、单株地上生物量下降较慢,分别为30.6%、35.0%;100～300mmol·L-1 NaCl处理下陇西种质叶片相对含水量降幅均最大,分别为6.2%、21.5%、30.3%;300mmol·L-1 NaCl浓度处理景泰种质可溶性糖含量上升最快,达4.4倍。与对照相比,各材料根总长、根表面积及根体积在300mmol·L-1 NaCl处理下均呈不同程度的下降,其中天水种质下降最快,陇西下降最慢;200和300mmol·L-1浓度处理下各材料叶绿素含量均小于低浓度处理和对照,且后者显著低于对照(P<0.05)。各扁蓿豆种质净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)随盐浓度的上升总体呈下降趋势,胞间CO2浓度(Ci)除陇西、临夏、宁县、土默特扁蓿豆种质随盐浓度的增加上升外,其余8份种质均先升后降。NaCl胁迫使各扁蓿豆种质光合作用受到不同程度的抑制。应用隶属函数法综合评价得出,参试种质种子苗期耐盐性强弱依次为景泰>永昌>土默特>宁县>天水>天祝>夏河>临夏>榆中>自选8号>陇西>镇原。
Twelve germplasms of Medicago ruthenica were treated with different concentrations of NaCl(0,100,200,300 mmol·L-1),and their salt tolerance at seedling stage was studied by sand culture.The results showed that,with an increase in NaCl concentration,the absolute plant height,aboveground biomass per plant,and leaf relative water content of the 12 populations of M.ruthenica gradually decreased while the soluble sugar content increased continuously.Compared with the control(CK),the mean decline in absolute plant height of Jingtai germplasm was 16.4%.The absolute height and aboveground biomass per plant of Zhenyuan germplasm decreased slowly by 30.6% and 35.0%,respectively.Under 100,200,and 300 mmol·L-1 treatments,the highest decline in leaf relative water content was observed in Longxi germplasm(6.2%,21.5%,and 30.3%,respectively).The increase in soluble sugar content of Jingtai germplasm was the fastest at 300 mmol·L-1 treatment and was up to 4.4 times the soluble sugar content of control.Compared with CK,the root total length,root surface area,and root volume decreased to a different degree on treatment with 300 mmol·L-1 NaCl in all the germplasm materials,of which the decline in Tianshui germplasm was the fastest and that in Longxi was the slowest.Additionally,the chlorophyll contents of all the materials treated with 200 mmol·L-1 NaCl and 300 mmol·L-1 NaCl were lower than those of the materials treated with lower NaCl concentrations,and the latter was significantly lower than that of CK(P<0.05).The net photosynthetic rate(Pn),transpiration rate(Tr),and stomatal conductance(Gs)of all M.ruthenica populations decreased with an increase in salt concentration.The intercellular CO2 concentration(Ci)of Longxi,Linxia,Ningxian,and Tumete germplasm increased with an increase in salt concentration;Ciof the remaining eight germplasms increased initially but decreased afterward.Photosynthesis in all the populations of M.ruthenica was inhibited differently under NaCl stress.Salt tolerance of the tested germplasm resources at seedling stage was comprehensive evaluated by the membership function method and ranked as Jingtai> Yongchang> Tumote>Ningxian>Tianshui>Tianzhu>Xiahe>Linxia>Yuzhong>No.8 Zixuan>Longxi>Zhenyuan.
Twelve germplasms of Medicago ruthenica were treated with different concentrations of NaCl(0,100,200,300 mmol·L-1),and their salt tolerance at seedling stage was studied by sand culture.The results showed that,with an increase in NaCl concentration,the absolute plant height,aboveground biomass per plant,and leaf relative water content of the 12 populations of M.ruthenica gradually decreased while the soluble sugar content increased continuously.Compared with the control(CK),the mean decline in absolute plant height of Jingtai germplasm was 16.4%.The absolute height and aboveground biomass per plant of Zhenyuan germplasm decreased slowly by 30.6% and 35.0%,respectively.Under 100,200,and 300 mmol·L-1 treatments,the highest decline in leaf relative water content was observed in Longxi germplasm(6.2%,21.5%,and 30.3%,respectively).The increase in soluble sugar content of Jingtai germplasm was the fastest at 300 mmol·L-1 treatment and was up to 4.4 times the soluble sugar content of control.Compared with CK,the root total length,root surface area,and root volume decreased to a different degree on treatment with 300 mmol·L-1 NaCl in all the germplasm materials,of which the decline in Tianshui germplasm was the fastest and that in Longxi was the slowest.Additionally,the chlorophyll contents of all the materials treated with 200 mmol·L-1 NaCl and 300 mmol·L-1 NaCl were lower than those of the materials treated with lower NaCl concentrations,and the latter was significantly lower than that of CK(P<0.05).The net photosynthetic rate(Pn),transpiration rate(Tr),and stomatal conductance(Gs)of all M.ruthenica populations decreased with an increase in salt concentration.The intercellular CO2 concentration(Ci)of Longxi,Linxia,Ningxian,and Tumete germplasm increased with an increase in salt concentration;Ciof the remaining eight germplasms increased initially but decreased afterward.Photosynthesis in all the populations of M.ruthenica was inhibited differently under NaCl stress.Salt tolerance of the tested germplasm resources at seedling stage was comprehensive evaluated by the membership function method and ranked as Jingtai> Yongchang> Tumote>Ningxian>Tianshui>Tianzhu>Xiahe>Linxia>Yuzhong>No.8 Zixuan>Longxi>Zhenyuan.
引文
[1]中国科学院中国植物志编辑委员会.中国植物志(第42卷第二分册).北京:科学出版社,1998:318-320.Editorial Committee of Chinese Journal of plant of Chinese Academy of Sciences.Flora of China(Vol.42,No.2).Beijing:Science Press,1998:318-320.
[2]贾慎修.中国饲用植物志(第一卷).北京:农业出版社,1987:337-340.JIA S X.Forage Floras of China(Volume 1).Beijing:Agriculture Press,1987:337-340.
[3]史万光,王照兰,杜建材,胡卉芳,杨勇.扁蓿豆不同品系种子发芽期耐盐性鉴定.中国草地学报,2008,30(1):40-44.SHI W G,WANG Z L,DU J C,HU H F,YANG Y.Evaluation the salt tolerance of Melilotoides ruthenicastrains in the germination period.Chinese Journal of Grassland,2008,30(1):40-44.
[4]韩海波,师文贵,李志勇,李鸿雁,李兴酉,刘磊,王晓娜.扁蓿豆的抗性研究进展.草业科学,2011,28(4):631-635.HAN H B,SHI W G,LI Z Y,LI H Y,LI X Y,LIU L,WANG X N.Research progress of resistance of Medicago ruthenica.Partacultural Science,2011,28(4):631-635.
[5]黄迎新,周道玮,岳秀泉,杨季云.扁蓿豆研究进展.草业科学,2007,24(12):34-39.HUANG Y X,ZHOU D W,YUE X Q,YANG J Y.Research progress of Medicago ruthenica.Partacultural Science,2007,24(12):34-39.
[6]陈陆军,徐长林,鱼小军,景媛媛,杨海磊,张建文.20份扁蓿豆材料在甘肃武威地区的生产性能与品质表现.草原与草坪,2015,35(4):5-11.CHEN L J,XU C L,YU X J,JING Y Y,YANG H L,ZHANG J W.Production performance and quality of 20 Medicago ruthenica germplasms in Wuwei City,Gansu Province.Grassland and Turf,2015,35(4):5-11.
[7]李鸿雁,李志勇,李红,蔡丽艳,张静萍,邢建军.用灰色关联度法综合评价扁蓿豆生产性能.草业科学,2012,29(11):1736-1742.LI H Y,LI Z Y,LI H,CAI L Y,ZHANG J P,XING J J.Comprehensive evaluation of production performance of Medicago ruthenica by grey correlation method.Partacultural Science,2012,29(11):1736-1742.
[8]郝林峰,李俊,李鸿雁,黄帆,王成海.3个生态区野生扁蓿豆种质资源表型多样性的主成分和聚类分析.北方农业学报,2017,45(3):6-12.HAO L F,LI J,LI H Y,HUANG F,WANG C H.Principal component and cluster analysis of phenotypic diversity of wild Medicago ruthenica germplasm resources in three ecological regions of Inner Mongolia.Journal of Northern Agriculture,2017,45(3):6-12.
[9]李志勇,李鸿雁,蔡丽艳,师文贵,黄帆,刘磊,解永风.中国扁蓿豆种质资源遗传多样性的ISSR标记与生态因子相关性分析.华北农学报,2014,29(6):94-100.LI Z Y,LI H Y,CAI L Y,SHI W G,HUANG F,LIU L,XIE Y F.Genetic diversity of ISSR markers and environment factors correlation analysis of Medicago ruthenicain China.Acta Agriculturae Boreali-Sinica,2014,29(6):94-100.
[10]李鸿雁,卢新雄,辛霞,李志勇,李俊,黄帆,刘磊.采用AFLP和SSR标记对扁蓿豆遗传多样性进行比较分析(英文).华北农学报,2017,32(2):124-130.LI H Y,LU X X,XIN X,LI Z Y,LI J,HUANG F,LIU L.Comparative analysis of genetic diversity of Medicago ruthenicain China using AFLP and SSR markers.Acta Agriculturae Boreali-Sinica,2017,32(2):124-130.
[11]李鸿雁,李志勇,辛霞,卢新雄,李俊,黄帆,刘磊.49份野生扁蓿豆种质资源的AFLP遗传多样性分析.中国草地学报,2016,38(4):20-26.LI H Y,LI Z Y,XIN X,LU X X,LI J,HUANG F,LIU L.Analysis of 49 Medicago ruthenica Trautv.germplasms based on AFLP molecular markers.Chinese Journal of Grassland,2016,38(4):20-26.
[12]石凤翎,郭晓霞,李红.扁蓿豆抗旱形态解剖结构观察与分析.干旱地区农业研究,2005,23(2):115-118.SHI F L,GUO X X,LI H.Examination and analysis of drought-resisting morphology and anatomy of Melilotoides ruthenica.Agricultural Research in the Arid Areas,2005,23(2):115-118.
[13]蔡丽艳,李志勇,孙启忠,李鸿雁,杨玉平,刘蕾.扁蓿豆萌发对干旱胁迫的响应及抗旱性评价.草业科学,2012,29(10):1553-1559.CAI L Y,LI Z Y,SUN Q Z,LI H Y,YANG Y P,LIU L.Response of seed germination of Melilotoides ruthenica to drought stress and its drought resistance evaluation.Partacultural Science,2012,29(10):1553-1559.
[14]张宇,石凤翎,云娜,高霞,伊风艳,蔡丽艳.扁蓿豆冷诱导基因差异表达分析.西北植物学报,2013,33(1):60-65.ZHANG Y,SHI F L,YUN N,GAO X,YI F Y,CAI L Y.cDNA-AFLP analysis of cold-induced genes in Medicago ruthenica.Acta Botanica Boreali-Occidentalia Sinica,2013,33(1):60-65.
[15]张建文,徐长林,鱼小军,景媛媛,肖红,张玉琴.九份扁蓿豆种子萌发期耐盐性研究.中国草地学报,2014,36(5):83-88.ZHANG J W,XU C L,YU X J,JING Y Y,XIAO H,ZHANG Y Q.Salt resistance of nine wild Medicago ruthenicaaccessions during seed germination stages.Chinese Journal of Grassland,2014,36(5):83-88.
[16]徐成体,德科加.用不同方法处理后直立型扁蓿豆种子的发芽效果.青海畜牧兽医杂志,1996,26(6):4-6.XU C L,DE K J.Germination of Melilotoides ruthenicus seed treated with different ways.Chinese Qinghai Journal of Animal and Veterinary Sciences,1996,26(6):4-6.
[17]刘建新,王鑫,贾海燕,李东波.黑麦草幼苗对NaCl和NaHCO3胁迫的光合响应差异.中国沙漠,2012,32(5):1342-1348.LIU J X,WANG X,JIA H Y,LI D B.Difference in photosynthetic response of ryegrass seedling to NaCl and NaHCO3stress.Journal of Desert Research,2012,32(5):1342-1348.
[18]邹琦.植物生理学实验指导.北京:中国农业出版社,2003.ZOU Q.Plant Physiology Experiment Instructor.Beijing:China Agriculture Press,2003.
[19]孙璐,周宇飞,李丰先,肖木辑,陶冶,许文娟,黄瑞冬.盐胁迫对高粱幼苗光合作用和荧光特性的影响.中国农业科学,2012,45(16):3265-3272.SUN L,ZHOU Y F,LI F X,XIAO M J,TAO Y,XU W J,HUANG R D.Impacts of salt stress on characteristics of photosynthesis and chlorophyll fluorescence of Sorghum seedlings.Scientia Agricultura Sinica,2012,45(16):3265-3272.
[20] MUNNS R,TESTER M.Mechanisms of salinity tolerance.Annual Review of Plant Biology,2008,59(1):651-681.
[21] VICENTE O,BOSCAIU M,NARANJO M,ESTRELLES E,BELLS J M,SORIANO P.Responses to salt stress in the halophyte Plantago crassifolia(Plantaginaceae).Journal of Arid Environments,2004,58(4):463-481.
[22]朱世杨,刘庆,张小玲,罗天宽,唐征,荆赞革,裘波音,吴海涛,徐谦.盐胁迫对花椰菜苗期幼苗生物量及脯氨酸含量的影响.西南农业学报,2016,29(1):148-152.ZHU S Y,LIU Q,ZHANG X L,LUO T K,TANG Z,JING Z G,QIU B Y,WU H T,XU Q.Effect of salt stress on biomass and proline content of cauliflower seedlings.Southwest China Journal of Agricultural Sciences,2016,29(1):148-152.
[23]韩冰,徐刚,郭世荣,贺超兴,孙艳军,高文瑞,李德翠,史珑燕.不同浓度盐胁迫对黄瓜幼苗生长和生理代谢的影响.江苏农业学报,2014,30(1):172-177.HAN B,XU G,GUO S R,HE C X,SUN Y J,GAO W R,LI D C,SHI L Y.Growth and physiological metabolism of cucumber seedlings in response to different concentrations of salt stress.Jiangsu Journal of Agricultural Sciences,2014,30(1):172-177.
[24]伍国强,贾姝,刘海龙,王春梅,李善家.盐胁迫对红豆草幼苗生长和离子积累及分配的影响.草业科学,2017,34(8):1661-1668.WU G Q,JIA S,LIU H L,WANG C M,LI S J.Effect of salt stress on growth,ion accumulation,and distribution in sainfoins seedlings.Partacultural Science,2017,34(8):1661-1668.
[25] SRINIVASRAO C,BENZIONI A,ESHEL A,WAISEL Y.Effects of salinity on root morphology and nutrient acquisition by faba beans(Vicia faba L.).Journal of the Indian Society of Soil Science,2004,52(2):184-191.
[26]黄依婷,郭鹏,许涵杰.盐胁迫对紫花苜蓿根系形态特征和水分利用效率的影响.黑龙江农业科学,2016,39(4):114-120.HUANG Y T,GUO P,XU H J.Effect of salt stress on root morphological characteristics and leaf water use efficiency in alfalfa.Heilongjiang Agriculture Sciences,2016,39(4):114-120.
[27]田淑慧,陶翠霞,张锋.20种药用植物的苗期耐盐性及其评价.山东农业科学,2017,49(8):49-54.TIAN S H,TAO C X,ZHANG F.Evaluation on salt tolerance of twenty medicinal plants at seedling stage.Shandong Agricultural Sciences,2017,49(8):49-54.
[28]张昆,李明娜,曹世豪,孙彦.白颖苔草对不同浓度NaCl胁迫的响应及其耐盐阈值.草业科学,2017,34(3):479-487.ZHANG K,LI M N,CAO S H,SUN Y.Response of carex rigescens to different NaCl concentrations and its salinity threshold calculation.Partacultural Science,2017,34(3):479-487.
[29]周赟,张林平,刘艳.盐胁迫对甘草幼苗生长及其生理生化特性的影响.北方农业学报,2017,45(2):1-6.ZHOU Y,ZHANG L P,LIU Y.Influence of salt stress on glycyrrhiza seedling growth and physiological and biochemical indexes.Journal of Northern Agriculture,2017,45(2):1-6.
[30]龙聪颖,邓辉茗,苏明洁,蔡仕珍,徐小明.3种盐胁迫对蓟幼苗生长及生理生化的影响.草业科学,2017,34(12):2484-2492.LONG C Y,DENG H M,SU M J,CAI S Z,XU X M.Effect of three salt stresses on growth,physiology,and biochemistry of Cirsium japonicum.Partacultural Science,2017,34(12):2484-2492.
[31]高彩婷,刘景辉,张玉芹,徐寿军,张玉霞.短期盐胁迫下燕麦幼苗的生理响应.草地学报,2017,25(2):337-343.GAO C X,LIU J H,ZHANG Y Q,XU S J,ZHANG Y X.Physiological responses of oats seedlings to short-term salt stress.Acta Agrestia Sinica,2017,25(2):337-343.
[32]卢瑞克,杨泽茂,戴志刚,唐蜻,谢冬微,许英,程超华,粟建光.黄麻耐盐性评价及盐胁迫下的生理响应.中国麻业科学,2017,39(4):195-203.LU R K,YANG Z M,DAI Z G,TANG Q,XIE D W,XU Y,CHENG C H,SU J G.Evaluation of salt tolerance and the physiological response of jute at seedling growth stages.Plant Fiber Sciences in China,2017,39(4):195-203.
[33]徐晨,凌风楼,徐克章,武志海,刘晓龙,安久海,赵兰坡.盐胁迫对不同水稻品种光合特性和生理生化特性的影响.中国水稻科学,2013,27(3):280-286.XU C,LING F L,XU K Z,WU Z H,LIU X L,AN J H,ZHAO L P.Effect of salt stress on photosynthetic characteristics and physiological and biochemical traits of different rice varieties.Chinese Journal of Rice Science,2013,27(3):280-286.
[34]刘国红,姜超强,刘兆普,梁明祥,殷祥贞,郑青松.盐胁迫对油菜幼苗生长和光合特征的影响.生态与农村环境学报,2012,28(2):157-164.LIU G H,JIANG C Q,LIU Z P,LIANG M X,YIN X Z,ZHENG Q S.Effects of salt stress on growth and photosynthetic traits of canola seedlings.Journal of Ecology and Rural Environment,2012,28(2):157-164.
[35]张潭,唐达,李思思,李倩,张震中,王池宇,谢守忠,贺康宁.盐碱胁迫对枸杞幼苗生物量积累和光合作用的影响.西北植物学报,2017,37(12):2474-2482.ZHANG T,TANG D,LI S S,LI Q,ZHANG Z Z,WANG C Y,XIE S Z,HE K N.Responses of growth and photosynthesis of Lycium barbarum L.seedling to salt-stress and alkali-stress.Acta Botanica Boreali-Occidentalia Sinica,2017,37(12):2474-2482.
[36]范方,张玉霞,姜健,杨宝灵,高明波.盐胁迫对紫花苜蓿生长及光合生理特性的影响.中国农学通报,2013,29(17):14-18.FAN F,ZHANG Y X,JIANG J,YANG B L,GAO M B.Effects of salt stress on the growth and photosynthetic physiological characteristics of alfalfa.Chinese Agricultural Science Bulletin,2013,29(17):14-18.
[37]刘倩,高娅妮,柳旭,王佺珍.植物对盐碱胁迫的响应机制研究进展.生态学报,2017,37(16):1-13.LIU Q,GAO Y N,LIU X,WANG Q Z.Review on the mechanisms of the response to salinity-alkalinity stress in plants.Acta Ecologica Sinica,2017,37(16):1-13.
[2]贾慎修.中国饲用植物志(第一卷).北京:农业出版社,1987:337-340.JIA S X.Forage Floras of China(Volume 1).Beijing:Agriculture Press,1987:337-340.
[3]史万光,王照兰,杜建材,胡卉芳,杨勇.扁蓿豆不同品系种子发芽期耐盐性鉴定.中国草地学报,2008,30(1):40-44.SHI W G,WANG Z L,DU J C,HU H F,YANG Y.Evaluation the salt tolerance of Melilotoides ruthenicastrains in the germination period.Chinese Journal of Grassland,2008,30(1):40-44.
[4]韩海波,师文贵,李志勇,李鸿雁,李兴酉,刘磊,王晓娜.扁蓿豆的抗性研究进展.草业科学,2011,28(4):631-635.HAN H B,SHI W G,LI Z Y,LI H Y,LI X Y,LIU L,WANG X N.Research progress of resistance of Medicago ruthenica.Partacultural Science,2011,28(4):631-635.
[5]黄迎新,周道玮,岳秀泉,杨季云.扁蓿豆研究进展.草业科学,2007,24(12):34-39.HUANG Y X,ZHOU D W,YUE X Q,YANG J Y.Research progress of Medicago ruthenica.Partacultural Science,2007,24(12):34-39.
[6]陈陆军,徐长林,鱼小军,景媛媛,杨海磊,张建文.20份扁蓿豆材料在甘肃武威地区的生产性能与品质表现.草原与草坪,2015,35(4):5-11.CHEN L J,XU C L,YU X J,JING Y Y,YANG H L,ZHANG J W.Production performance and quality of 20 Medicago ruthenica germplasms in Wuwei City,Gansu Province.Grassland and Turf,2015,35(4):5-11.
[7]李鸿雁,李志勇,李红,蔡丽艳,张静萍,邢建军.用灰色关联度法综合评价扁蓿豆生产性能.草业科学,2012,29(11):1736-1742.LI H Y,LI Z Y,LI H,CAI L Y,ZHANG J P,XING J J.Comprehensive evaluation of production performance of Medicago ruthenica by grey correlation method.Partacultural Science,2012,29(11):1736-1742.
[8]郝林峰,李俊,李鸿雁,黄帆,王成海.3个生态区野生扁蓿豆种质资源表型多样性的主成分和聚类分析.北方农业学报,2017,45(3):6-12.HAO L F,LI J,LI H Y,HUANG F,WANG C H.Principal component and cluster analysis of phenotypic diversity of wild Medicago ruthenica germplasm resources in three ecological regions of Inner Mongolia.Journal of Northern Agriculture,2017,45(3):6-12.
[9]李志勇,李鸿雁,蔡丽艳,师文贵,黄帆,刘磊,解永风.中国扁蓿豆种质资源遗传多样性的ISSR标记与生态因子相关性分析.华北农学报,2014,29(6):94-100.LI Z Y,LI H Y,CAI L Y,SHI W G,HUANG F,LIU L,XIE Y F.Genetic diversity of ISSR markers and environment factors correlation analysis of Medicago ruthenicain China.Acta Agriculturae Boreali-Sinica,2014,29(6):94-100.
[10]李鸿雁,卢新雄,辛霞,李志勇,李俊,黄帆,刘磊.采用AFLP和SSR标记对扁蓿豆遗传多样性进行比较分析(英文).华北农学报,2017,32(2):124-130.LI H Y,LU X X,XIN X,LI Z Y,LI J,HUANG F,LIU L.Comparative analysis of genetic diversity of Medicago ruthenicain China using AFLP and SSR markers.Acta Agriculturae Boreali-Sinica,2017,32(2):124-130.
[11]李鸿雁,李志勇,辛霞,卢新雄,李俊,黄帆,刘磊.49份野生扁蓿豆种质资源的AFLP遗传多样性分析.中国草地学报,2016,38(4):20-26.LI H Y,LI Z Y,XIN X,LU X X,LI J,HUANG F,LIU L.Analysis of 49 Medicago ruthenica Trautv.germplasms based on AFLP molecular markers.Chinese Journal of Grassland,2016,38(4):20-26.
[12]石凤翎,郭晓霞,李红.扁蓿豆抗旱形态解剖结构观察与分析.干旱地区农业研究,2005,23(2):115-118.SHI F L,GUO X X,LI H.Examination and analysis of drought-resisting morphology and anatomy of Melilotoides ruthenica.Agricultural Research in the Arid Areas,2005,23(2):115-118.
[13]蔡丽艳,李志勇,孙启忠,李鸿雁,杨玉平,刘蕾.扁蓿豆萌发对干旱胁迫的响应及抗旱性评价.草业科学,2012,29(10):1553-1559.CAI L Y,LI Z Y,SUN Q Z,LI H Y,YANG Y P,LIU L.Response of seed germination of Melilotoides ruthenica to drought stress and its drought resistance evaluation.Partacultural Science,2012,29(10):1553-1559.
[14]张宇,石凤翎,云娜,高霞,伊风艳,蔡丽艳.扁蓿豆冷诱导基因差异表达分析.西北植物学报,2013,33(1):60-65.ZHANG Y,SHI F L,YUN N,GAO X,YI F Y,CAI L Y.cDNA-AFLP analysis of cold-induced genes in Medicago ruthenica.Acta Botanica Boreali-Occidentalia Sinica,2013,33(1):60-65.
[15]张建文,徐长林,鱼小军,景媛媛,肖红,张玉琴.九份扁蓿豆种子萌发期耐盐性研究.中国草地学报,2014,36(5):83-88.ZHANG J W,XU C L,YU X J,JING Y Y,XIAO H,ZHANG Y Q.Salt resistance of nine wild Medicago ruthenicaaccessions during seed germination stages.Chinese Journal of Grassland,2014,36(5):83-88.
[16]徐成体,德科加.用不同方法处理后直立型扁蓿豆种子的发芽效果.青海畜牧兽医杂志,1996,26(6):4-6.XU C L,DE K J.Germination of Melilotoides ruthenicus seed treated with different ways.Chinese Qinghai Journal of Animal and Veterinary Sciences,1996,26(6):4-6.
[17]刘建新,王鑫,贾海燕,李东波.黑麦草幼苗对NaCl和NaHCO3胁迫的光合响应差异.中国沙漠,2012,32(5):1342-1348.LIU J X,WANG X,JIA H Y,LI D B.Difference in photosynthetic response of ryegrass seedling to NaCl and NaHCO3stress.Journal of Desert Research,2012,32(5):1342-1348.
[18]邹琦.植物生理学实验指导.北京:中国农业出版社,2003.ZOU Q.Plant Physiology Experiment Instructor.Beijing:China Agriculture Press,2003.
[19]孙璐,周宇飞,李丰先,肖木辑,陶冶,许文娟,黄瑞冬.盐胁迫对高粱幼苗光合作用和荧光特性的影响.中国农业科学,2012,45(16):3265-3272.SUN L,ZHOU Y F,LI F X,XIAO M J,TAO Y,XU W J,HUANG R D.Impacts of salt stress on characteristics of photosynthesis and chlorophyll fluorescence of Sorghum seedlings.Scientia Agricultura Sinica,2012,45(16):3265-3272.
[20] MUNNS R,TESTER M.Mechanisms of salinity tolerance.Annual Review of Plant Biology,2008,59(1):651-681.
[21] VICENTE O,BOSCAIU M,NARANJO M,ESTRELLES E,BELLS J M,SORIANO P.Responses to salt stress in the halophyte Plantago crassifolia(Plantaginaceae).Journal of Arid Environments,2004,58(4):463-481.
[22]朱世杨,刘庆,张小玲,罗天宽,唐征,荆赞革,裘波音,吴海涛,徐谦.盐胁迫对花椰菜苗期幼苗生物量及脯氨酸含量的影响.西南农业学报,2016,29(1):148-152.ZHU S Y,LIU Q,ZHANG X L,LUO T K,TANG Z,JING Z G,QIU B Y,WU H T,XU Q.Effect of salt stress on biomass and proline content of cauliflower seedlings.Southwest China Journal of Agricultural Sciences,2016,29(1):148-152.
[23]韩冰,徐刚,郭世荣,贺超兴,孙艳军,高文瑞,李德翠,史珑燕.不同浓度盐胁迫对黄瓜幼苗生长和生理代谢的影响.江苏农业学报,2014,30(1):172-177.HAN B,XU G,GUO S R,HE C X,SUN Y J,GAO W R,LI D C,SHI L Y.Growth and physiological metabolism of cucumber seedlings in response to different concentrations of salt stress.Jiangsu Journal of Agricultural Sciences,2014,30(1):172-177.
[24]伍国强,贾姝,刘海龙,王春梅,李善家.盐胁迫对红豆草幼苗生长和离子积累及分配的影响.草业科学,2017,34(8):1661-1668.WU G Q,JIA S,LIU H L,WANG C M,LI S J.Effect of salt stress on growth,ion accumulation,and distribution in sainfoins seedlings.Partacultural Science,2017,34(8):1661-1668.
[25] SRINIVASRAO C,BENZIONI A,ESHEL A,WAISEL Y.Effects of salinity on root morphology and nutrient acquisition by faba beans(Vicia faba L.).Journal of the Indian Society of Soil Science,2004,52(2):184-191.
[26]黄依婷,郭鹏,许涵杰.盐胁迫对紫花苜蓿根系形态特征和水分利用效率的影响.黑龙江农业科学,2016,39(4):114-120.HUANG Y T,GUO P,XU H J.Effect of salt stress on root morphological characteristics and leaf water use efficiency in alfalfa.Heilongjiang Agriculture Sciences,2016,39(4):114-120.
[27]田淑慧,陶翠霞,张锋.20种药用植物的苗期耐盐性及其评价.山东农业科学,2017,49(8):49-54.TIAN S H,TAO C X,ZHANG F.Evaluation on salt tolerance of twenty medicinal plants at seedling stage.Shandong Agricultural Sciences,2017,49(8):49-54.
[28]张昆,李明娜,曹世豪,孙彦.白颖苔草对不同浓度NaCl胁迫的响应及其耐盐阈值.草业科学,2017,34(3):479-487.ZHANG K,LI M N,CAO S H,SUN Y.Response of carex rigescens to different NaCl concentrations and its salinity threshold calculation.Partacultural Science,2017,34(3):479-487.
[29]周赟,张林平,刘艳.盐胁迫对甘草幼苗生长及其生理生化特性的影响.北方农业学报,2017,45(2):1-6.ZHOU Y,ZHANG L P,LIU Y.Influence of salt stress on glycyrrhiza seedling growth and physiological and biochemical indexes.Journal of Northern Agriculture,2017,45(2):1-6.
[30]龙聪颖,邓辉茗,苏明洁,蔡仕珍,徐小明.3种盐胁迫对蓟幼苗生长及生理生化的影响.草业科学,2017,34(12):2484-2492.LONG C Y,DENG H M,SU M J,CAI S Z,XU X M.Effect of three salt stresses on growth,physiology,and biochemistry of Cirsium japonicum.Partacultural Science,2017,34(12):2484-2492.
[31]高彩婷,刘景辉,张玉芹,徐寿军,张玉霞.短期盐胁迫下燕麦幼苗的生理响应.草地学报,2017,25(2):337-343.GAO C X,LIU J H,ZHANG Y Q,XU S J,ZHANG Y X.Physiological responses of oats seedlings to short-term salt stress.Acta Agrestia Sinica,2017,25(2):337-343.
[32]卢瑞克,杨泽茂,戴志刚,唐蜻,谢冬微,许英,程超华,粟建光.黄麻耐盐性评价及盐胁迫下的生理响应.中国麻业科学,2017,39(4):195-203.LU R K,YANG Z M,DAI Z G,TANG Q,XIE D W,XU Y,CHENG C H,SU J G.Evaluation of salt tolerance and the physiological response of jute at seedling growth stages.Plant Fiber Sciences in China,2017,39(4):195-203.
[33]徐晨,凌风楼,徐克章,武志海,刘晓龙,安久海,赵兰坡.盐胁迫对不同水稻品种光合特性和生理生化特性的影响.中国水稻科学,2013,27(3):280-286.XU C,LING F L,XU K Z,WU Z H,LIU X L,AN J H,ZHAO L P.Effect of salt stress on photosynthetic characteristics and physiological and biochemical traits of different rice varieties.Chinese Journal of Rice Science,2013,27(3):280-286.
[34]刘国红,姜超强,刘兆普,梁明祥,殷祥贞,郑青松.盐胁迫对油菜幼苗生长和光合特征的影响.生态与农村环境学报,2012,28(2):157-164.LIU G H,JIANG C Q,LIU Z P,LIANG M X,YIN X Z,ZHENG Q S.Effects of salt stress on growth and photosynthetic traits of canola seedlings.Journal of Ecology and Rural Environment,2012,28(2):157-164.
[35]张潭,唐达,李思思,李倩,张震中,王池宇,谢守忠,贺康宁.盐碱胁迫对枸杞幼苗生物量积累和光合作用的影响.西北植物学报,2017,37(12):2474-2482.ZHANG T,TANG D,LI S S,LI Q,ZHANG Z Z,WANG C Y,XIE S Z,HE K N.Responses of growth and photosynthesis of Lycium barbarum L.seedling to salt-stress and alkali-stress.Acta Botanica Boreali-Occidentalia Sinica,2017,37(12):2474-2482.
[36]范方,张玉霞,姜健,杨宝灵,高明波.盐胁迫对紫花苜蓿生长及光合生理特性的影响.中国农学通报,2013,29(17):14-18.FAN F,ZHANG Y X,JIANG J,YANG B L,GAO M B.Effects of salt stress on the growth and photosynthetic physiological characteristics of alfalfa.Chinese Agricultural Science Bulletin,2013,29(17):14-18.
[37]刘倩,高娅妮,柳旭,王佺珍.植物对盐碱胁迫的响应机制研究进展.生态学报,2017,37(16):1-13.LIU Q,GAO Y N,LIU X,WANG Q Z.Review on the mechanisms of the response to salinity-alkalinity stress in plants.Acta Ecologica Sinica,2017,37(16):1-13.