气滞血瘀型慢性输卵管炎症大鼠模型的建立与评价
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摘要
目的探究气滞血瘀型慢性输卵管炎模型的构建方法,考察其可行性并对该模型进行初步评价,为进一步实验研究奠定基础。方法选择SD大鼠,采用不可预知的反复慢性应激刺激(声、光、电、夹尾刺激)法及输卵管手术注菌操作来构建气滞血瘀型慢性输卵管炎症模型,并通过症候评价、表征与病理学观察及血流变学各项指标检测,对气滞血瘀型慢性输卵管炎症动物模型的建立进行初步评价。结果与模型组相比较,复合模型组症候总积分显著上升(P<0. 01),组织形态观察中输卵管80%出现严重血管肿胀,60%出现严重管壁增厚;病理学形态分析中发现复合模型组输卵管管腔内较多炎细胞浸润,粘膜上皮细胞脱落,并伴有输卵管周围大量炎细胞浸润;血流变学检测中得到复合模型组的血浆黏度、全血黏度、还原黏度、红细胞刚性指数与聚集指数升高极为显著(P<0. 01)。结论综合评价分析发现气滞血瘀型慢性输卵管炎模型大鼠基本上符合血瘀证候的变化特点及慢性炎症病理表现,与人类在该病中的发病临床表现相似,造模方法可行。
Objective To explore a method to construct a rat model with qi stagnation and blood stasis chronic salpingitis,to study its feasibility and establish a method for its primary evaluation,and to lay the foundations for future experiments. Methods SD rats were subjected to predictable repeated stress stimulation( e.g.,sound,light,electricity,and clip tail stimulation) and bacteria were injected into the oviduct to construct the model with qi stagnation and blood stasis chronic salpingitis. Symptoms,and observation,pathological,and blood rheology indexes were used to primarily evaluate the rat model. Results Compared with the control group,the combined qi stagnation and blood stasis with chronic salpingitis model group's total mark rose significantly Qi( P< 0. 01),80% of the oviduct blood vessels exhibited swelling and distension,and 60% of their walls showed thickening. Pathological analysis revealed the infiltration of inflammatory cells in the oviduct lumens of the rat model with qi stagnation and blood stasis chronic salpingitis,and epithelial mucosal ablation was accompanied by substantial inflammatory cell infiltration surrounding the oviduct. Blood rheology analyses demonstrated very significantly increased plasma viscosity,whole blood viscosity,reduction viscosity,and erythrocyte rigidity index and aggregation index in the combined rat model group( P < 0. 01). Conclusions Our comprehensiveanalysis reveals that the rat model with qi stagnation and blood stasis chronic salpingitis is roughly consistent with the dynamic characteristics of blood stasis and the pathological status of chronic inflammation,being similar to the human clinical manifestations. The method for constructing this model is feasible.
Objective To explore a method to construct a rat model with qi stagnation and blood stasis chronic salpingitis,to study its feasibility and establish a method for its primary evaluation,and to lay the foundations for future experiments. Methods SD rats were subjected to predictable repeated stress stimulation( e.g.,sound,light,electricity,and clip tail stimulation) and bacteria were injected into the oviduct to construct the model with qi stagnation and blood stasis chronic salpingitis. Symptoms,and observation,pathological,and blood rheology indexes were used to primarily evaluate the rat model. Results Compared with the control group,the combined qi stagnation and blood stasis with chronic salpingitis model group's total mark rose significantly Qi( P< 0. 01),80% of the oviduct blood vessels exhibited swelling and distension,and 60% of their walls showed thickening. Pathological analysis revealed the infiltration of inflammatory cells in the oviduct lumens of the rat model with qi stagnation and blood stasis chronic salpingitis,and epithelial mucosal ablation was accompanied by substantial inflammatory cell infiltration surrounding the oviduct. Blood rheology analyses demonstrated very significantly increased plasma viscosity,whole blood viscosity,reduction viscosity,and erythrocyte rigidity index and aggregation index in the combined rat model group( P < 0. 01). Conclusions Our comprehensiveanalysis reveals that the rat model with qi stagnation and blood stasis chronic salpingitis is roughly consistent with the dynamic characteristics of blood stasis and the pathological status of chronic inflammation,being similar to the human clinical manifestations. The method for constructing this model is feasible.
引文
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[2]赵广兴,王春田,马宝璋,等.大鼠输卵管炎性不孕症模型的建立[J].中国比较医学杂志,2004,14(1):23-26.
[3]任建勋,林成仁,王敏,等.多因素整合建立气滞血瘀动物模型研究[J].中药药理与临床,2007,23(5):210-211.
[4]王少贤,白明华,陈家旭,等.关于建立中医证候模型评价量表的思考[J].中华中医药杂志,2011,26(3):531-534.
[5]田金洲,王永炎,时晶,等.证候模型研究的思路[J].北京中医药大学学报,2005,28(5):25-27.
[6]王琨.血瘀证动物模型体表特征及生物学基础研究[D].北京:北京中医药大学,2008.
[7]朱龙.浅议“以方测证”法在中医动物模型研究中的运用[J].甘肃中医,2009,22(12):18-19.
[8]任建勋,刘建勋,林成仁,等.冠心病不稳定性心绞痛患者气滞血瘀证和气虚血瘀证的生物学基础比较分析[J].中国中西医结合杂志,2010,30(4):352-356.
[9]刘国生,王桐生,龙子江,等.盆腔炎冲剂对大鼠慢性盆腔炎模型的影响[J].中国实验方剂学杂志,2004,10(3):24-26.
[10] Von Tempelhoff GF,Niemann F,Schneider DM,et al. Blood rheology during chemotherapy in patients with ovarian cancer[J].Thromb Res,1998,90(2):73-82.
[11]向海燕,王羽伦,侯连兵,等.金刚藤分散片对大鼠慢性盆腔炎血液流变学与病理组织形态学的影响[J].現代食品與藥品雜誌,2007,17(2):38-41.
[12] Von Tempelhoff GF,Velten E,Yilmaz A,et al. Blood rheology at term in normal pregnancy and in patients with adverse outcome events[J]. Clin Hemorheol Microcirc,2009,42(2):127-139.