川陈皮素对高血压大鼠的血压调节作用及机制
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摘要
目的探讨川陈皮素对高血压大鼠的血压调节作用及分子机制。方法使用L-NNA制造内皮损伤型高血压大鼠模型,给予不同浓度的川陈皮素,分别在造模前、造模后、给药14天及给药28天时测量大鼠血压、心率和体重的改变。处死给药28天大鼠,取动脉血,使用ELISA法测量NO、EDHF、ANG-2和PGI2含量。结果川陈皮素给药14天及28天后,高血压大鼠血压明显下调,且ELISA结果显示高血压大鼠动脉血中NO和PGI2的含量增加,但EDHF和ANG-2含量无影响。结论川陈皮素可影响血管内皮细胞功能,提高NO和PGI2的含量,使高血压大鼠血压下降。
Objective To investigate role of nobiletin on the blood pressure regulation in hypertension rats and its mechanism. Methods The hypertensive rat model with endothelial injury was prepared by L-NNA and then was injected by the different contents of nobiletin. Changes of artery pressure, heart rate, and weight before and after model establishment at 14 days and 28 days after model establishment were detected. All of rats that had been injected for 28 days were killed and their artery plasma were collected. The contents of NO, EDHF, ANG-2 and PGI2 were measured. Results The blood pressure decreased obviously after treated with nobiletin for 14 days and 28 days. And ELISA results showed that the total NO and PGI2 contents in artery blood increased, but the PGI2 contents had no change. Conclusion Nobiletin may have an important effect on the function of the vascular endothelium and increase the PGI2 and NO contents, causing to lower artery pressure.
Objective To investigate role of nobiletin on the blood pressure regulation in hypertension rats and its mechanism. Methods The hypertensive rat model with endothelial injury was prepared by L-NNA and then was injected by the different contents of nobiletin. Changes of artery pressure, heart rate, and weight before and after model establishment at 14 days and 28 days after model establishment were detected. All of rats that had been injected for 28 days were killed and their artery plasma were collected. The contents of NO, EDHF, ANG-2 and PGI2 were measured. Results The blood pressure decreased obviously after treated with nobiletin for 14 days and 28 days. And ELISA results showed that the total NO and PGI2 contents in artery blood increased, but the PGI2 contents had no change. Conclusion Nobiletin may have an important effect on the function of the vascular endothelium and increase the PGI2 and NO contents, causing to lower artery pressure.
引文
[1] 吴兆苏,霍勇,王文,等.中国高血压患者教育指南[J].中国医学前沿杂志(电子版),2014,6(3):78-110.
[2] Sp N,Kang DY,Joung Y,et al.Nobiletin inhibits angiogenesis by regulating Src/FAK/STAT3-Mediated signaling through PXN in ER(+) breast cancer cells[J].Int J Mol Sci,2017,18(5):935-948.
[3] Moon JY,Cho SK.Nobiletin induces protective autophagy accompanied by ER-Stress mediated apoptosis in human gastric cancer SNU-16 cells[J].Molecules,2016,21(7):914-927.
[4] Zhang X,Zheng K,Li C,et al.Nobiletin inhibits invasion via inhibiting AKT/GSK3beta/beta-catenin signaling pathway in Slug-expressing glioma cells[J].Oncol Rep,2017,37(5):2847-2856.
[5] Chien SY,Hsieh MJ.Nobiletin inhibits invasion and migration of human nasopharyngeal carcinoma cell lines by involving ERK1/2 and transcriptional inhibition of MMP-2[J].Expert Opin Ther Targets,2015,19(3):307-320.
[6] Lu WJ,Lin KC,Liu CP,et al.Prevention of arterial thrombosis by nobiletin:in vitro and in vivo studies[J].J Nutr Biochem,2016,28(10):1-8.
[7] Parkar NA,Bhatt LK,Addepalli V.Efficacy of nobiletin,a citrus flavonoid,in the treatment of the cardiovascular dysfunction of diabetes in rats[J].Food Funct,2016,7(7):3121-3129.
[8] Chan YC,Leung FP,Yao X,et al.Raloxifene modulates pulmonary vascular reactivity in spontaneously hypertensive rats[J].J Cardiovasc Pharmacol,2007,49(6):355-361.
[9] Hermann M,Camici G,Fratton A,et al.Differential effects of selective cyclooxygenase-2 inhibitors on endothelial function in salt-induced hypertension[J].Circulation,2009,17(5):S243.
[10] Jimenez R,Lopez-Sepulveda R,Kadmiri M,et al.Polyphenols restore endothelial function in DOCA-salt hypertension:role of endothelin-1 and NADPH oxidase[J].Free Radic Biol Med,2007,43(3):462-473.
[11] Johnson FK,Johnson RA,Peyton KJ,et al.Arginase inhibition restores arteriolar endothelial function in Dahl rats with salt-induced hypertension[J].AJP Regulatory Integrative and Comparative Physiology,2005,288(4):R1057-R1062.
[12] Nitenberg A.Hypertension,endothelial dysfunction and cardiovascular risk[J].Archives Des Maladies Du Coeur Et Des Vaisseaux,2006,99(10):915-921.
[13] Filip M,Maciag J,Nosalski,et al.Endothelial dysfunction related to oxidative stress and inflammation in perivascular adipose tissue[J].Postepy Biochem,2012,58(2):186-194.
[14] Wallace SML,Yasmin,Mceniery CM,et al.Isolated systolic hypertension is characterized by increased aortic stiffness and endothelial dysfunction[J].Hypertension,2007,50(1):228-233.
[2] Sp N,Kang DY,Joung Y,et al.Nobiletin inhibits angiogenesis by regulating Src/FAK/STAT3-Mediated signaling through PXN in ER(+) breast cancer cells[J].Int J Mol Sci,2017,18(5):935-948.
[3] Moon JY,Cho SK.Nobiletin induces protective autophagy accompanied by ER-Stress mediated apoptosis in human gastric cancer SNU-16 cells[J].Molecules,2016,21(7):914-927.
[4] Zhang X,Zheng K,Li C,et al.Nobiletin inhibits invasion via inhibiting AKT/GSK3beta/beta-catenin signaling pathway in Slug-expressing glioma cells[J].Oncol Rep,2017,37(5):2847-2856.
[5] Chien SY,Hsieh MJ.Nobiletin inhibits invasion and migration of human nasopharyngeal carcinoma cell lines by involving ERK1/2 and transcriptional inhibition of MMP-2[J].Expert Opin Ther Targets,2015,19(3):307-320.
[6] Lu WJ,Lin KC,Liu CP,et al.Prevention of arterial thrombosis by nobiletin:in vitro and in vivo studies[J].J Nutr Biochem,2016,28(10):1-8.
[7] Parkar NA,Bhatt LK,Addepalli V.Efficacy of nobiletin,a citrus flavonoid,in the treatment of the cardiovascular dysfunction of diabetes in rats[J].Food Funct,2016,7(7):3121-3129.
[8] Chan YC,Leung FP,Yao X,et al.Raloxifene modulates pulmonary vascular reactivity in spontaneously hypertensive rats[J].J Cardiovasc Pharmacol,2007,49(6):355-361.
[9] Hermann M,Camici G,Fratton A,et al.Differential effects of selective cyclooxygenase-2 inhibitors on endothelial function in salt-induced hypertension[J].Circulation,2009,17(5):S243.
[10] Jimenez R,Lopez-Sepulveda R,Kadmiri M,et al.Polyphenols restore endothelial function in DOCA-salt hypertension:role of endothelin-1 and NADPH oxidase[J].Free Radic Biol Med,2007,43(3):462-473.
[11] Johnson FK,Johnson RA,Peyton KJ,et al.Arginase inhibition restores arteriolar endothelial function in Dahl rats with salt-induced hypertension[J].AJP Regulatory Integrative and Comparative Physiology,2005,288(4):R1057-R1062.
[12] Nitenberg A.Hypertension,endothelial dysfunction and cardiovascular risk[J].Archives Des Maladies Du Coeur Et Des Vaisseaux,2006,99(10):915-921.
[13] Filip M,Maciag J,Nosalski,et al.Endothelial dysfunction related to oxidative stress and inflammation in perivascular adipose tissue[J].Postepy Biochem,2012,58(2):186-194.
[14] Wallace SML,Yasmin,Mceniery CM,et al.Isolated systolic hypertension is characterized by increased aortic stiffness and endothelial dysfunction[J].Hypertension,2007,50(1):228-233.